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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ANKLE2-NCOR2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ANKLE2-NCOR2
	FusionPDB ID: 4499
	FusionGDB2.0 ID: 4499
		Hgene	Tgene
	Gene symbol	ANKLE2	NCOR2
	Gene ID	23141	9612
	Gene name	ankyrin repeat and LEM domain containing 2	nuclear receptor corepressor 2
	Synonyms	KIAA0692\|LEMD7\|Lem4\|MCPH16	CTG26\|N-CoR2\|SMAP270\|SMRT\|SMRTE\|SMRTE-tau\|TNRC14\|TRAC\|TRAC-1\|TRAC1
	Cytomap	12q24.33	12q24.31
	Type of gene	protein-coding	protein-coding
	Description	ankyrin repeat and LEM domain-containing protein 2LEM domain containing 7LEM domain-containing protein 4	nuclear receptor corepressor 2CTG repeat protein 26T3 receptor-associating factorsilencing mediator for retinoid and thyroid hormone receptorsthyroid-, retinoic-acid-receptor-associated corepressor
	Modification date	20200313	20200313
	UniProtAcc	Q86XL3	Q9Y618
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000337516, ENST00000357997, ENST00000539605, ENST00000542282, ENST00000542374, ENST00000542657,	ENST00000356219, ENST00000397355, ENST00000404121, ENST00000404621, ENST00000405201, ENST00000429285,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	8 X 7 X 7=392	19 X 18 X 11=3762
	# samples	9	19
	** MAII score	log2(9/392*10)=-2.12285674778553 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(19/3762*10)=-4.30742852519225 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ANKLE2 [Title/Abstract] AND NCOR2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ANKLE2(133327229)-NCOR2(124812179), # samples:3
Anticipated loss of major functional domain due to fusion event.	ANKLE2-NCOR2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ANKLE2-NCOR2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ANKLE2-NCOR2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ANKLE2-NCOR2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ANKLE2	GO:0007417	central nervous system development	25259927
Hgene	ANKLE2	GO:0035307	positive regulation of protein dephosphorylation	22770216
Hgene	ANKLE2	GO:0042326	negative regulation of phosphorylation	22770216
Hgene	ANKLE2	GO:0043066	negative regulation of apoptotic process	25259927

Fusion gene breakpoints across ANKLE2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across NCOR2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LGG	TCGA-DU-8167-01A	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-
ChimerDB4	LGG	TCGA-DU-8167	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000539605	ANKLE2	chr12	133327229	-	ENST00000405201	NCOR2	chr12	124812179	-	8920	7346	6670	7932	420
ENST00000539605	ANKLE2	chr12	133327229	-	ENST00000404621	NCOR2	chr12	124812179	-	8782	7346	6670	7794	374
ENST00000539605	ANKLE2	chr12	133327229	-	ENST00000404121	NCOR2	chr12	124812179	-	8919	7346	6670	7932	420
ENST00000539605	ANKLE2	chr12	133327229	-	ENST00000356219	NCOR2	chr12	124812179	-	8919	7346	6670	7932	420
ENST00000539605	ANKLE2	chr12	133327229	-	ENST00000397355	NCOR2	chr12	124812179	-	8781	7346	6670	7794	374
ENST00000539605	ANKLE2	chr12	133327229	-	ENST00000429285	NCOR2	chr12	124812179	-	8737	7346	6670	7932	420
ENST00000357997	ANKLE2	chr12	133327229	-	ENST00000405201	NCOR2	chr12	124812179	-	2511	937	90	1523	477
ENST00000357997	ANKLE2	chr12	133327229	-	ENST00000404621	NCOR2	chr12	124812179	-	2373	937	90	1385	431
ENST00000357997	ANKLE2	chr12	133327229	-	ENST00000404121	NCOR2	chr12	124812179	-	2510	937	90	1523	477
ENST00000357997	ANKLE2	chr12	133327229	-	ENST00000356219	NCOR2	chr12	124812179	-	2510	937	90	1523	477
ENST00000357997	ANKLE2	chr12	133327229	-	ENST00000397355	NCOR2	chr12	124812179	-	2372	937	90	1385	431
ENST00000357997	ANKLE2	chr12	133327229	-	ENST00000429285	NCOR2	chr12	124812179	-	2328	937	90	1523	477
ENST00000337516	ANKLE2	chr12	133327229	-	ENST00000405201	NCOR2	chr12	124812179	-	2488	914	67	1500	477
ENST00000337516	ANKLE2	chr12	133327229	-	ENST00000404621	NCOR2	chr12	124812179	-	2350	914	67	1362	431
ENST00000337516	ANKLE2	chr12	133327229	-	ENST00000404121	NCOR2	chr12	124812179	-	2487	914	67	1500	477
ENST00000337516	ANKLE2	chr12	133327229	-	ENST00000356219	NCOR2	chr12	124812179	-	2487	914	67	1500	477
ENST00000337516	ANKLE2	chr12	133327229	-	ENST00000397355	NCOR2	chr12	124812179	-	2349	914	67	1362	431
ENST00000337516	ANKLE2	chr12	133327229	-	ENST00000429285	NCOR2	chr12	124812179	-	2305	914	67	1500	477

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000539605	ENST00000405201	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.002898205	0.9971017
ENST00000539605	ENST00000404621	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.002659046	0.997341
ENST00000539605	ENST00000404121	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.003074395	0.99692565
ENST00000539605	ENST00000356219	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.003074395	0.99692565
ENST00000539605	ENST00000397355	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.002684328	0.9973157
ENST00000539605	ENST00000429285	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.002995546	0.99700445
ENST00000357997	ENST00000405201	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.01402758	0.9859724
ENST00000357997	ENST00000404621	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.016634079	0.98336595
ENST00000357997	ENST00000404121	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.014043653	0.9859563
ENST00000357997	ENST00000356219	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.014043653	0.9859563
ENST00000357997	ENST00000397355	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.016634546	0.9833655
ENST00000357997	ENST00000429285	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.01654744	0.9834525
ENST00000337516	ENST00000405201	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.013951171	0.9860488
ENST00000337516	ENST00000404621	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.016896749	0.9831033
ENST00000337516	ENST00000404121	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.013972684	0.98602736
ENST00000337516	ENST00000356219	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.013972684	0.98602736
ENST00000337516	ENST00000397355	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.016899824	0.9831002
ENST00000337516	ENST00000429285	ANKLE2	chr12	133327229	-	NCOR2	chr12	124812179	-	0.016480653	0.9835194

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>4499_4499_1_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000337516_NCOR2_chr12_124812179_ENST00000356219_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_2_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000337516_NCOR2_chr12_124812179_ENST00000397355_length(amino acids)=431AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLT

--------------------------------------------------------------

>4499_4499_3_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000337516_NCOR2_chr12_124812179_ENST00000404121_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_4_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000337516_NCOR2_chr12_124812179_ENST00000404621_length(amino acids)=431AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLT

--------------------------------------------------------------

>4499_4499_5_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000337516_NCOR2_chr12_124812179_ENST00000405201_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_6_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000337516_NCOR2_chr12_124812179_ENST00000429285_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_7_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000357997_NCOR2_chr12_124812179_ENST00000356219_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_8_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000357997_NCOR2_chr12_124812179_ENST00000397355_length(amino acids)=431AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLT

--------------------------------------------------------------

>4499_4499_9_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000357997_NCOR2_chr12_124812179_ENST00000404121_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_10_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000357997_NCOR2_chr12_124812179_ENST00000404621_length(amino acids)=431AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLT

--------------------------------------------------------------

>4499_4499_11_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000357997_NCOR2_chr12_124812179_ENST00000405201_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_12_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000357997_NCOR2_chr12_124812179_ENST00000429285_length(amino acids)=477AA_BP=280
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK
CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP
SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV
KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG
GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP

--------------------------------------------------------------

>4499_4499_13_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000539605_NCOR2_chr12_124812179_ENST00000356219_length(amino acids)=420AA_BP=223
MGLGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTD
QAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRF
KAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANA
FNPLNASASLPAAMPITAADGRSDHTLTSPGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSA

--------------------------------------------------------------

>4499_4499_14_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000539605_NCOR2_chr12_124812179_ENST00000397355_length(amino acids)=374AA_BP=223
MGLGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTD
QAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRF
KAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGGGGKAKVSGRPSSRK
AKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGPLAGPHHAWDEEPK

--------------------------------------------------------------

>4499_4499_15_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000539605_NCOR2_chr12_124812179_ENST00000404121_length(amino acids)=420AA_BP=223
MGLGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTD
QAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRF
KAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANA
FNPLNASASLPAAMPITAADGRSDHTLTSPGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSA

--------------------------------------------------------------

>4499_4499_16_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000539605_NCOR2_chr12_124812179_ENST00000404621_length(amino acids)=374AA_BP=223
MGLGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTD
QAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRF
KAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGGGGKAKVSGRPSSRK
AKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGPLAGPHHAWDEEPK

--------------------------------------------------------------

>4499_4499_17_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000539605_NCOR2_chr12_124812179_ENST00000405201_length(amino acids)=420AA_BP=223
MGLGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTD
QAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRF
KAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANA
FNPLNASASLPAAMPITAADGRSDHTLTSPGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSA

--------------------------------------------------------------

>4499_4499_18_ANKLE2-NCOR2_ANKLE2_chr12_133327229_ENST00000539605_NCOR2_chr12_124812179_ENST00000429285_length(amino acids)=420AA_BP=223
MGLGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTD
QAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRF
KAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANA
FNPLNASASLPAAMPITAADGRSDHTLTSPGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSA

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:133327229/chr12:124812179)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ANKLE2 Q86XL3	NCOR2 Q9Y618
FUNCTION: Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit (PubMed:22770216). Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly (PubMed:22770216). May regulate nuclear localization of VRK1 in non-dividing cells (PubMed:31735666). It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (PubMed:22770216). Involved in brain development (PubMed:25259927). {ECO:0000269\|PubMed:22770216, ECO:0000269\|PubMed:25259927, ECO:0000269\|PubMed:31735666}.	FUNCTION: Transcriptional corepressor (PubMed:20812024). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). {ECO:0000269\|PubMed:18212045, ECO:0000269\|PubMed:20812024, ECO:0000269\|PubMed:23911289}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000337516

69_113

282.3333333333333

649.0

Domain

LEM

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000357997

69_113

282.3333333333333

939.0

Domain

LEM

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000337516

1_12

282.3333333333333

649.0

Topological domain

Lumenal

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000357997

1_12

282.3333333333333

939.0

Topological domain

Lumenal

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000337516

13_32

282.3333333333333

649.0

Transmembrane

Helical%3B Signal-anchor for type III membrane protein

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000357997

13_32

282.3333333333333

939.0

Transmembrane

Helical%3B Signal-anchor for type III membrane protein

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

2476_2479

2326.3333333333335

2522.0

Compositional bias

Note=Poly-Pro

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

2339_2343

2326.3333333333335

2522.0

Motif

Note=CORNR box of ID2

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542282

69_113

294.0

Domain

LEM

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542657

69_113

294.0

Domain

LEM

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000337516

411_440

282.3333333333333

649.0

Repeat

Note=ANK

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000357997

411_440

282.3333333333333

939.0

Repeat

Note=ANK

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542282

411_440

294.0

Repeat

Note=ANK

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542657

411_440

294.0

Repeat

Note=ANK

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000337516

33_938

282.3333333333333

649.0

Topological domain

Cytoplasmic

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000357997

33_938

282.3333333333333

939.0

Topological domain

Cytoplasmic

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542282

1_12

294.0

Topological domain

Lumenal

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542282

33_938

294.0

Topological domain

Cytoplasmic

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542657

1_12

294.0

Topological domain

Lumenal

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542657

33_938

294.0

Topological domain

Cytoplasmic

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542282

13_32

294.0

Transmembrane

Helical%3B Signal-anchor for type III membrane protein

Hgene

ANKLE2

chr12:133327229

chr12:124812179

ENST00000542657

13_32

294.0

Transmembrane

Helical%3B Signal-anchor for type III membrane protein

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

174_215

2326.3333333333335

2522.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

522_561

2326.3333333333335

2522.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

1384_1389

2326.3333333333335

2522.0

Compositional bias

Note=Poly-Pro

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

1839_1843

2326.3333333333335

2522.0

Compositional bias

Note=Poly-Gly

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

494_510

2326.3333333333335

2522.0

Compositional bias

Note=Poly-Gln

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

682_685

2326.3333333333335

2522.0

Compositional bias

Note=Poly-Lys

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

778_820

2326.3333333333335

2522.0

Compositional bias

Note=Pro-rich

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

995_1003

2326.3333333333335

2522.0

Compositional bias

Note=Poly-Pro

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

427_478

2326.3333333333335

2522.0

Domain

SANT 1

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

610_661

2326.3333333333335

2522.0

Domain

SANT 2

Tgene

NCOR2

chr12:133327229

chr12:124812179

ENST00000356219

2136_2140

2326.3333333333335

2522.0

Motif

Note=CORNR box of ID1

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>999_ANKLE2_133327229_NCOR2_124812179_999_ANKLE2_133327229_NCOR2_124812179_ranked_0.pdb	ANKLE2	133327229	133327229	ENST00000429285	NCOR2	chr12	124812179	-	MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLK CGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPV KTAPLFSNDRLKGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGG GKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGP	477

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ANKLE2_pLDDT.png

NCOR2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ANKLE2
NCOR2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	NCOR2	chr12:133327229	chr12:124812179	ENST00000356219		44	48	254_312	2326.3333333333335	2522.0	SIN3A/B

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Related Drugs to ANKLE2-NCOR2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to ANKLE2-NCOR2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source