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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ANLN-EEPD1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ANLN-EEPD1
	FusionPDB ID: 4833
	FusionGDB2.0 ID: 4833
		Hgene	Tgene
	Gene symbol	ANLN	EEPD1
	Gene ID	54443	80820
	Gene name	anillin actin binding protein	endonuclease/exonuclease/phosphatase family domain containing 1
	Synonyms	FSGS8\|Scraps\|scra	HSPC107
	Cytomap	7p14.2	7p14.2
	Type of gene	protein-coding	protein-coding
	Description	anillin	endonuclease/exonuclease/phosphatase family domain-containing protein 1
	Modification date	20200313	20200313
	UniProtAcc	Q9NQW6	Q7L9B9
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000265748, ENST00000396068, ENST00000495714,	ENST00000242108, ENST00000534978,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 8 X 7=336	6 X 7 X 5=210
	# samples	10	9
	** MAII score	log2(10/336*10)=-1.74846123300404 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/210*10)=-1.22239242133645 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ANLN [Title/Abstract] AND EEPD1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ANLN(36459088)-EEPD1(36320723), # samples:2 ANLN(36436028)-EEPD1(36336602), # samples:2 EEPD1(36327386)-ANLN(36435875), # samples:1
Anticipated loss of major functional domain due to fusion event.	ANLN-EEPD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ANLN-EEPD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ANLN-EEPD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ANLN-EEPD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EEPD1-ANLN seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ANLN	GO:0000281	mitotic cytokinesis	10931866

Fusion gene breakpoints across ANLN (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across EEPD1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUAD	TCGA-44-6778-01A	ANLN	chr7	36436028	-	EEPD1	chr7	36336602	+
ChimerDB4	LUAD	TCGA-44-6778-01A	ANLN	chr7	36436028	+	EEPD1	chr7	36336602	+
ChimerDB4	LUAD	TCGA-44-6778	ANLN	chr7	36436028	+	EEPD1	chr7	36336601	+
ChimerDB4	OV	TCGA-24-2023-01A	ANLN	chr7	36459088	+	EEPD1	chr7	36320724	+
ChimerDB4	OV	TCGA-24-2023	ANLN	chr7	36459088	+	EEPD1	chr7	36320723	+
ChimerDB4	READ	TCGA-AG-A036	ANLN	chr7	36459088	+	EEPD1	chr7	36320723	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000265748	ANLN	chr7	36459088	+	ENST00000242108	EEPD1	chr7	36320723	+	5207	2090	221	2869	882
ENST00000265748	ANLN	chr7	36459088	+	ENST00000534978	EEPD1	chr7	36320723	+	3389	2090	221	2869	882
ENST00000396068	ANLN	chr7	36459088	+	ENST00000242108	EEPD1	chr7	36320723	+	5081	1964	206	2743	845
ENST00000396068	ANLN	chr7	36459088	+	ENST00000534978	EEPD1	chr7	36320723	+	3263	1964	206	2743	845
ENST00000265748	ANLN	chr7	36459088	+	ENST00000242108	EEPD1	chr7	36320724	+	5207	2090	221	2869	882
ENST00000265748	ANLN	chr7	36459088	+	ENST00000534978	EEPD1	chr7	36320724	+	3389	2090	221	2869	882
ENST00000396068	ANLN	chr7	36459088	+	ENST00000242108	EEPD1	chr7	36320724	+	5081	1964	206	2743	845
ENST00000396068	ANLN	chr7	36459088	+	ENST00000534978	EEPD1	chr7	36320724	+	3263	1964	206	2743	845

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000265748	ENST00000242108	ANLN	chr7	36459088	+	EEPD1	chr7	36320723	+	0.001522356	0.99847764
ENST00000265748	ENST00000534978	ANLN	chr7	36459088	+	EEPD1	chr7	36320723	+	0.003116228	0.9968837
ENST00000396068	ENST00000242108	ANLN	chr7	36459088	+	EEPD1	chr7	36320723	+	0.002077828	0.99792224
ENST00000396068	ENST00000534978	ANLN	chr7	36459088	+	EEPD1	chr7	36320723	+	0.004457626	0.99554235
ENST00000265748	ENST00000242108	ANLN	chr7	36459088	+	EEPD1	chr7	36320724	+	0.001522356	0.99847764
ENST00000265748	ENST00000534978	ANLN	chr7	36459088	+	EEPD1	chr7	36320724	+	0.003116228	0.9968837
ENST00000396068	ENST00000242108	ANLN	chr7	36459088	+	EEPD1	chr7	36320724	+	0.002077828	0.99792224
ENST00000396068	ENST00000534978	ANLN	chr7	36459088	+	EEPD1	chr7	36320724	+	0.004457626	0.99554235

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>4833_4833_1_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000265748_EEPD1_chr7_36320723_ENST00000242108_length(amino acids)=882AA_BP=623
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKV
EQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQ
PTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGFLWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAA
LTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVIILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKS

--------------------------------------------------------------

>4833_4833_2_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000265748_EEPD1_chr7_36320723_ENST00000534978_length(amino acids)=882AA_BP=623
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKV
EQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQ
PTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGFLWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAA
LTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVIILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKS

--------------------------------------------------------------

>4833_4833_3_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000265748_EEPD1_chr7_36320724_ENST00000242108_length(amino acids)=882AA_BP=623
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKV
EQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQ
PTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGFLWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAA
LTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVIILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKS

--------------------------------------------------------------

>4833_4833_4_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000265748_EEPD1_chr7_36320724_ENST00000534978_length(amino acids)=882AA_BP=623
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKV
EQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQ
PTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGFLWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAA
LTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVIILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKS

--------------------------------------------------------------

>4833_4833_5_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000396068_EEPD1_chr7_36320723_ENST00000242108_length(amino acids)=845AA_BP=586
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKEVIREIEMSVDDDDINSSKVINDLFSDVLEEGE
LDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQPTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGF
LWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAALTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVI
ILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKSLKKVFTGHWAVVREGLTNPWIPDNWSWGGVASEHCPV

--------------------------------------------------------------

>4833_4833_6_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000396068_EEPD1_chr7_36320723_ENST00000534978_length(amino acids)=845AA_BP=586
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKEVIREIEMSVDDDDINSSKVINDLFSDVLEEGE
LDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQPTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGF
LWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAALTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVI
ILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKSLKKVFTGHWAVVREGLTNPWIPDNWSWGGVASEHCPV

--------------------------------------------------------------

>4833_4833_7_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000396068_EEPD1_chr7_36320724_ENST00000242108_length(amino acids)=845AA_BP=586
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKEVIREIEMSVDDDDINSSKVINDLFSDVLEEGE
LDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQPTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGF
LWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAALTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVI
ILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKSLKKVFTGHWAVVREGLTNPWIPDNWSWGGVASEHCPV

--------------------------------------------------------------

>4833_4833_8_ANLN-EEPD1_ANLN_chr7_36459088_ENST00000396068_EEPD1_chr7_36320724_ENST00000534978_length(amino acids)=845AA_BP=586
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES
TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA
ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN
KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD
KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA
EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKEVIREIEMSVDDDDINSSKVINDLFSDVLEEGE
LDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQPTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGF
LWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAALTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVI
ILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKSLKKVFTGHWAVVREGLTNPWIPDNWSWGGVASEHCPV

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:36459088/chr7:36320723)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ANLN Q9NQW6	EEPD1 Q7L9B9
FUNCTION: Required for cytokinesis (PubMed:16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed:23870127). May play a significant role in podocyte cell migration (PubMed:24676636). {ECO:0000269\|PubMed:10931866, ECO:0000269\|PubMed:12479805, ECO:0000269\|PubMed:15496454, ECO:0000269\|PubMed:16040610, ECO:0000269\|PubMed:16357138, ECO:0000269\|PubMed:23870127, ECO:0000269\|PubMed:24676636}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000265748

569_604

623.0

1125.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000265748

569_604

623.0

1125.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000265748

1_45

57.333333333333336

1125.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000396068

1_45

57.333333333333336

1088.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000265748

1_45

57.333333333333336

1125.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000396068

1_45

57.333333333333336

1088.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000265748

1_230

623.0

1125.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000265748

1_45

623.0

1125.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000396068

1_230

586.0

1088.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000396068

1_45

586.0

1088.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000265748

1_230

623.0

1125.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000265748

1_45

623.0

1125.0

Region

Note=Required for ubiquitination

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000396068

1_230

586.0

1088.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000396068

1_45

586.0

1088.0

Region

Note=Required for ubiquitination

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000265748

569_604

57.333333333333336

1125.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000396068

569_604

57.333333333333336

1088.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000265748

569_604

57.333333333333336

1125.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000396068

569_604

57.333333333333336

1088.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000396068

569_604

586.0

1088.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000396068

569_604

586.0

1088.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000265748

983_1107

57.333333333333336

1125.0

Domain

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000396068

983_1107

57.333333333333336

1088.0

Domain

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000265748

983_1107

57.333333333333336

1125.0

Domain

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000396068

983_1107

57.333333333333336

1088.0

Domain

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000265748

983_1107

623.0

1125.0

Domain

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000396068

983_1107

586.0

1088.0

Domain

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000265748

983_1107

623.0

1125.0

Domain

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000396068

983_1107

586.0

1088.0

Domain

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000265748

1_230

57.333333333333336

1125.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000265748

730_1124

57.333333333333336

1125.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000396068

1_230

57.333333333333336

1088.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36436028

chr7:36336601

ENST00000396068

730_1124

57.333333333333336

1088.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000265748

1_230

57.333333333333336

1125.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000265748

730_1124

57.333333333333336

1125.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000396068

1_230

57.333333333333336

1088.0

Region

Note=Nuclear localization

Hgene

ANLN

chr7:36436028

chr7:36336602

ENST00000396068

730_1124

57.333333333333336

1088.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000265748

730_1124

623.0

1125.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36459088

chr7:36320723

ENST00000396068

730_1124

586.0

1088.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000265748

730_1124

623.0

1125.0

Region

Note=Localization to the cleavage furrow

Hgene

ANLN

chr7:36459088

chr7:36320724

ENST00000396068

730_1124

586.0

1088.0

Region

Note=Localization to the cleavage furrow

Tgene

EEPD1

chr7:36436028

chr7:36336601

ENST00000242108

38_67

438.3333333333333

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36436028

chr7:36336601

ENST00000534978

38_67

438.3333333333333

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36436028

chr7:36336602

ENST00000242108

38_67

438.3333333333333

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36436028

chr7:36336602

ENST00000534978

38_67

438.3333333333333

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36459088

chr7:36320723

ENST00000242108

38_67

310.0

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36459088

chr7:36320723

ENST00000534978

38_67

310.0

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36459088

chr7:36320724

ENST00000242108

38_67

310.0

570.0

Domain

Note=HhH

Tgene

EEPD1

chr7:36459088

chr7:36320724

ENST00000534978

38_67

310.0

570.0

Domain

Note=HhH

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1670_ANLN_36459088_EEPD1_36320723_ranked_0.pdb	ANLN	36459088	36459088	ENST00000534978	EEPD1	chr7	36320723	+	MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVES TSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKA ASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLN KALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKD KSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSA EKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKV EQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPEFCTELNQ PTLPNIRKWKGPRGCWKAVVAEKPSSQLQKGAGYAGFLWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAA LTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVIILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKS	882

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ANLN_pLDDT.png

EEPD1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ANLN
EEPD1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	ANLN	chr7:36436028	chr7:36336601	ENST00000265748	+	2	24	1_155	57.333333333333336	1125.0	CD2AP
Hgene	ANLN	chr7:36436028	chr7:36336601	ENST00000396068	+	2	23	1_155	57.333333333333336	1088.0	CD2AP
Hgene	ANLN	chr7:36436028	chr7:36336602	ENST00000265748	+	2	24	1_155	57.333333333333336	1125.0	CD2AP
Hgene	ANLN	chr7:36436028	chr7:36336602	ENST00000396068	+	2	23	1_155	57.333333333333336	1088.0	CD2AP
Hgene	ANLN	chr7:36436028	chr7:36336601	ENST00000265748	+	2	24	231_676	57.333333333333336	1125.0	F-actin
Hgene	ANLN	chr7:36436028	chr7:36336601	ENST00000396068	+	2	23	231_676	57.333333333333336	1088.0	F-actin
Hgene	ANLN	chr7:36436028	chr7:36336602	ENST00000265748	+	2	24	231_676	57.333333333333336	1125.0	F-actin
Hgene	ANLN	chr7:36436028	chr7:36336602	ENST00000396068	+	2	23	231_676	57.333333333333336	1088.0	F-actin
Hgene	ANLN	chr7:36459088	chr7:36320723	ENST00000265748	+	10	24	231_676	623.0	1125.0	F-actin
Hgene	ANLN	chr7:36459088	chr7:36320723	ENST00000396068	+	9	23	231_676	586.0	1088.0	F-actin
Hgene	ANLN	chr7:36459088	chr7:36320724	ENST00000265748	+	10	24	231_676	623.0	1125.0	F-actin
Hgene	ANLN	chr7:36459088	chr7:36320724	ENST00000396068	+	9	23	231_676	586.0	1088.0	F-actin

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Related Drugs to ANLN-EEPD1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to ANLN-EEPD1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source