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Fusion Protein:MAP2K4-PRR11 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: MAP2K4-PRR11 | FusionPDB ID: 51214 | FusionGDB2.0 ID: 51214 | Hgene | Tgene | Gene symbol | MAP2K4 | PRR11 | Gene ID | 6416 | 55771 |
| Gene name | mitogen-activated protein kinase kinase 4 | proline rich 11 | |
| Synonyms | JNKK|JNKK1|MAPKK4|MEK4|MKK4|PRKMK4|SAPKK-1|SAPKK1|SEK1|SERK1|SKK1 | - | |
| Cytomap | 17p12 | 17q22 | |
| Type of gene | protein-coding | protein-coding | |
| Description | dual specificity mitogen-activated protein kinase kinase 4JNK-activated kinase 1JNK-activating kinase 1MAP kinase kinase 4MAPK/ERK kinase 4MAPKK 4MEK 4SAPK/ERK kinase 1c-Jun N-terminal kinase kinase 1stress-activated protein kinase kinase 1 | proline-rich protein 11transcription repressor of MHCII | |
| Modification date | 20200329 | 20200313 | |
| UniProtAcc | P45985 | . | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000353533, ENST00000415385, ENST00000581941, | ENST00000262293, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 10 X 4 X 7=280 | 7 X 5 X 5=175 |
| # samples | 13 | 8 | |
| ** MAII score | log2(13/280*10)=-1.10691520391651 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(8/175*10)=-1.12928301694497 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: MAP2K4 [Title/Abstract] AND PRR11 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | MAP2K4(11958308)-PRR11(57262414), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | MAP2K4-PRR11 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MAP2K4-PRR11 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MAP2K4-PRR11 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MAP2K4-PRR11 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | PRR11 | GO:0051726 | regulation of cell cycle | 23246489 |
| Fusion gene breakpoints across MAP2K4 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across PRR11 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | ESCA | TCGA-L5-A4OU | MAP2K4 | chr17 | 11958308 | + | PRR11 | chr17 | 57262414 | + |
| ChimerDB4 | ESCA | TCGA-L5-A4OU | MAP2K4 | chr17 | 11958308 | + | PRR11 | chr17 | 57262415 | + |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000353533 | MAP2K4 | chr17 | 11958308 | + | ENST00000262293 | PRR11 | chr17 | 57262414 | + | 2173 | 281 | 63 | 1235 | 390 |
| ENST00000415385 | MAP2K4 | chr17 | 11958308 | + | ENST00000262293 | PRR11 | chr17 | 57262414 | + | 2196 | 304 | 53 | 1258 | 401 |
| ENST00000353533 | MAP2K4 | chr17 | 11958308 | + | ENST00000262293 | PRR11 | chr17 | 57262415 | + | 2173 | 281 | 63 | 1235 | 390 |
| ENST00000415385 | MAP2K4 | chr17 | 11958308 | + | ENST00000262293 | PRR11 | chr17 | 57262415 | + | 2196 | 304 | 53 | 1258 | 401 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000353533 | ENST00000262293 | MAP2K4 | chr17 | 11958308 | + | PRR11 | chr17 | 57262414 | + | 0.060400706 | 0.9395993 |
| ENST00000415385 | ENST00000262293 | MAP2K4 | chr17 | 11958308 | + | PRR11 | chr17 | 57262414 | + | 0.037580583 | 0.9624194 |
| ENST00000353533 | ENST00000262293 | MAP2K4 | chr17 | 11958308 | + | PRR11 | chr17 | 57262415 | + | 0.060400706 | 0.9395993 |
| ENST00000415385 | ENST00000262293 | MAP2K4 | chr17 | 11958308 | + | PRR11 | chr17 | 57262415 | + | 0.037580583 | 0.9624194 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >51214_51214_1_MAP2K4-PRR11_MAP2K4_chr17_11958308_ENST00000353533_PRR11_chr17_57262414_ENST00000262293_length(amino acids)=390AA_BP=73 MAAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIVGISSIDISQSRSWLTS SWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKDTIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISESSSCPSCGQTCH MSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPLAPVLLRKPSLAKALQAGPLKKDGPMQITVKDLLTVKLKKT QSLDEKRKLIPSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLRKVDVERSPGGTPLTNKENMETGTGLTPVMT -------------------------------------------------------------- >51214_51214_2_MAP2K4-PRR11_MAP2K4_chr17_11958308_ENST00000353533_PRR11_chr17_57262415_ENST00000262293_length(amino acids)=390AA_BP=73 MAAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIVGISSIDISQSRSWLTS SWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKDTIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISESSSCPSCGQTCH MSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPLAPVLLRKPSLAKALQAGPLKKDGPMQITVKDLLTVKLKKT QSLDEKRKLIPSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLRKVDVERSPGGTPLTNKENMETGTGLTPVMT -------------------------------------------------------------- >51214_51214_3_MAP2K4-PRR11_MAP2K4_chr17_11958308_ENST00000415385_PRR11_chr17_57262414_ENST00000262293_length(amino acids)=401AA_BP=84 MAAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGFQINFCEKAQSKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIVGISSI DISQSRSWLTSSWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKDTIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISES SSCPSCGQTCHMSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPLAPVLLRKPSLAKALQAGPLKKDGPMQITV KDLLTVKLKKTQSLDEKRKLIPSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLRKVDVERSPGGTPLTNKENM -------------------------------------------------------------- >51214_51214_4_MAP2K4-PRR11_MAP2K4_chr17_11958308_ENST00000415385_PRR11_chr17_57262415_ENST00000262293_length(amino acids)=401AA_BP=84 MAAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGFQINFCEKAQSKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIVGISSI DISQSRSWLTSSWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKDTIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISES SSCPSCGQTCHMSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPLAPVLLRKPSLAKALQAGPLKKDGPMQITV KDLLTVKLKKTQSLDEKRKLIPSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLRKVDVERSPGGTPLTNKENM -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:11958308/chr17:57262414) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| MAP2K4 | . |
| FUNCTION: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. {ECO:0000269|PubMed:7716521}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000353533 | + | 2 | 11 | 5_19 | 72.66666666666667 | 400.0 | Compositional bias | Note=Gly/Ser-rich |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000415385 | + | 3 | 12 | 5_19 | 83.66666666666667 | 411.0 | Compositional bias | Note=Gly/Ser-rich |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000353533 | + | 2 | 11 | 5_19 | 72.66666666666667 | 400.0 | Compositional bias | Note=Gly/Ser-rich |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000415385 | + | 3 | 12 | 5_19 | 83.66666666666667 | 411.0 | Compositional bias | Note=Gly/Ser-rich |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000353533 | + | 2 | 11 | 37_52 | 72.66666666666667 | 400.0 | Region | Note=D domain |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000415385 | + | 3 | 12 | 37_52 | 83.66666666666667 | 411.0 | Region | Note=D domain |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000353533 | + | 2 | 11 | 37_52 | 72.66666666666667 | 400.0 | Region | Note=D domain |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000415385 | + | 3 | 12 | 37_52 | 83.66666666666667 | 411.0 | Region | Note=D domain |
| Tgene | PRR11 | chr17:11958308 | chr17:57262414 | ENST00000262293 | 1 | 10 | 174_202 | 42.666666666666664 | 361.0 | Compositional bias | Note=Pro-rich | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262415 | ENST00000262293 | 1 | 10 | 174_202 | 42.666666666666664 | 361.0 | Compositional bias | Note=Pro-rich | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262414 | ENST00000262293 | 1 | 10 | 285_291 | 42.666666666666664 | 361.0 | Motif | Note=Phosphodegron | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262414 | ENST00000262293 | 1 | 10 | 296_304 | 42.666666666666664 | 361.0 | Motif | Note=D-box | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262414 | ENST00000262293 | 1 | 10 | 316_318 | 42.666666666666664 | 361.0 | Motif | Note=KEN box | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262414 | ENST00000262293 | 1 | 10 | 325_330 | 42.666666666666664 | 361.0 | Motif | Note=Phosphodegron | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262415 | ENST00000262293 | 1 | 10 | 285_291 | 42.666666666666664 | 361.0 | Motif | Note=Phosphodegron | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262415 | ENST00000262293 | 1 | 10 | 296_304 | 42.666666666666664 | 361.0 | Motif | Note=D-box | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262415 | ENST00000262293 | 1 | 10 | 316_318 | 42.666666666666664 | 361.0 | Motif | Note=KEN box | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262415 | ENST00000262293 | 1 | 10 | 325_330 | 42.666666666666664 | 361.0 | Motif | Note=Phosphodegron |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000353533 | + | 2 | 11 | 102_367 | 72.66666666666667 | 400.0 | Domain | Protein kinase |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000415385 | + | 3 | 12 | 102_367 | 83.66666666666667 | 411.0 | Domain | Protein kinase |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000353533 | + | 2 | 11 | 102_367 | 72.66666666666667 | 400.0 | Domain | Protein kinase |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000415385 | + | 3 | 12 | 102_367 | 83.66666666666667 | 411.0 | Domain | Protein kinase |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000353533 | + | 2 | 11 | 108_116 | 72.66666666666667 | 400.0 | Nucleotide binding | ATP |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000415385 | + | 3 | 12 | 108_116 | 83.66666666666667 | 411.0 | Nucleotide binding | ATP |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000353533 | + | 2 | 11 | 108_116 | 72.66666666666667 | 400.0 | Nucleotide binding | ATP |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000415385 | + | 3 | 12 | 108_116 | 83.66666666666667 | 411.0 | Nucleotide binding | ATP |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000353533 | + | 2 | 11 | 364_387 | 72.66666666666667 | 400.0 | Region | Note=DVD domain |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262414 | ENST00000415385 | + | 3 | 12 | 364_387 | 83.66666666666667 | 411.0 | Region | Note=DVD domain |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000353533 | + | 2 | 11 | 364_387 | 72.66666666666667 | 400.0 | Region | Note=DVD domain |
| Hgene | MAP2K4 | chr17:11958308 | chr17:57262415 | ENST00000415385 | + | 3 | 12 | 364_387 | 83.66666666666667 | 411.0 | Region | Note=DVD domain |
| Tgene | PRR11 | chr17:11958308 | chr17:57262414 | ENST00000262293 | 1 | 10 | 34_41 | 42.666666666666664 | 361.0 | Compositional bias | Note=Poly-Pro | |
| Tgene | PRR11 | chr17:11958308 | chr17:57262415 | ENST00000262293 | 1 | 10 | 34_41 | 42.666666666666664 | 361.0 | Compositional bias | Note=Poly-Pro |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| MAP2K4 | |
| PRR11 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to MAP2K4-PRR11 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to MAP2K4-PRR11 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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