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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MCC-DBN1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MCC-DBN1
	FusionPDB ID: 52152
	FusionGDB2.0 ID: 52152
		Hgene	Tgene
	Gene symbol	MCC	DBN1
	Gene ID	4163	1627
	Gene name	MCC regulator of WNT signaling pathway	drebrin 1
	Synonyms	MCC1	D0S117E
	Cytomap	5q22.2	5q35.3
	Type of gene	protein-coding	protein-coding
	Description	colorectal mutant cancer proteinMCC, WNT signaling pathway regulatormutated in colorectal cancers	drebrindevelopmentally-regulated brain proteindrebrin Adrebrin Edrebrin E2
	Modification date	20200313	20200320
	UniProtAcc	Q9HCC0	Q16643
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000302475, ENST00000514701, ENST00000408903, ENST00000515367,	ENST00000393563, ENST00000512501, ENST00000292385, ENST00000309007, ENST00000393565,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	11 X 8 X 5=440	10 X 9 X 7=630
	# samples	12	10
	** MAII score	log2(12/440*10)=-1.87446911791614 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(10/630*10)=-2.65535182861255 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MCC [Title/Abstract] AND DBN1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MCC(112630026)-DBN1(176887704), # samples:3
Anticipated loss of major functional domain due to fusion event.	MCC-DBN1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MCC-DBN1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MCC	GO:0045184	establishment of protein localization	18591935
Hgene	MCC	GO:0050680	negative regulation of epithelial cell proliferation	18591935
Hgene	MCC	GO:0090090	negative regulation of canonical Wnt signaling pathway	18591935
Tgene	DBN1	GO:0051220	cytoplasmic sequestering of protein	28966017

Fusion gene breakpoints across MCC (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across DBN1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LIHC	TCGA-BC-A10T-01A	MCC	chr5	112630026	-	DBN1	chr5	176887556	-
ChimerDB4	LIHC	TCGA-BC-A10T-01A	MCC	chr5	112630026	-	DBN1	chr5	176887704	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000302475	MCC	chr5	112630026	-	ENST00000309007	DBN1	chr5	176887704	-	2625	621	564	1799	411
ENST00000302475	MCC	chr5	112630026	-	ENST00000292385	DBN1	chr5	176887704	-	2306	621	564	1799	411
ENST00000302475	MCC	chr5	112630026	-	ENST00000393565	DBN1	chr5	176887704	-	2435	621	564	1937	457
ENST00000302475	MCC	chr5	112630026	-	ENST00000309007	DBN1	chr5	176887556	-	2565	621	564	1739	391
ENST00000302475	MCC	chr5	112630026	-	ENST00000292385	DBN1	chr5	176887556	-	2246	621	564	1739	391
ENST00000302475	MCC	chr5	112630026	-	ENST00000393565	DBN1	chr5	176887556	-	2375	621	564	1877	437
ENST00000302475	MCC	chr5	112630026	-	ENST00000393563	DBN1	chr5	176887556	-	1806	621	564	1739	391
ENST00000302475	MCC	chr5	112630026	-	ENST00000512501	DBN1	chr5	176887556	-	2257	621	564	1769	401

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000302475	ENST00000309007	MCC	chr5	112630026	-	DBN1	chr5	176887704	-	0.00876442	0.9912356
ENST00000302475	ENST00000292385	MCC	chr5	112630026	-	DBN1	chr5	176887704	-	0.010533752	0.98946625
ENST00000302475	ENST00000393565	MCC	chr5	112630026	-	DBN1	chr5	176887704	-	0.012428736	0.98757124
ENST00000302475	ENST00000309007	MCC	chr5	112630026	-	DBN1	chr5	176887556	-	0.011445856	0.9885542
ENST00000302475	ENST00000292385	MCC	chr5	112630026	-	DBN1	chr5	176887556	-	0.013888639	0.9861114
ENST00000302475	ENST00000393565	MCC	chr5	112630026	-	DBN1	chr5	176887556	-	0.025791978	0.974208
ENST00000302475	ENST00000393563	MCC	chr5	112630026	-	DBN1	chr5	176887556	-	0.014480311	0.98551977
ENST00000302475	ENST00000512501	MCC	chr5	112630026	-	DBN1	chr5	176887556	-	0.046184443	0.9538155

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>52152_52152_1_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887556_ENST00000292385_length(amino acids)=391AA_BP=19
MNSGVAMKYGNDSSAELSEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPV
AEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEP
ATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCA
TLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPP

--------------------------------------------------------------

>52152_52152_2_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887556_ENST00000309007_length(amino acids)=391AA_BP=19
MNSGVAMKYGNDSSAELSEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPV
AEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEP
ATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCA
TLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPP

--------------------------------------------------------------

>52152_52152_3_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887556_ENST00000393563_length(amino acids)=391AA_BP=19
MNSGVAMKYGNDSSAELSEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPV
AEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEP
ATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCA
TLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPP

--------------------------------------------------------------

>52152_52152_4_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887556_ENST00000393565_length(amino acids)=437AA_BP=19
MNSGVAMKYGNDSSAELSEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPF
PYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAA
APQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGN
GEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQE

--------------------------------------------------------------

>52152_52152_5_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887556_ENST00000512501_length(amino acids)=401AA_BP=19
MNSGVAMKYGNDSSAELSEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPV
AEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEP
ATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCA
TLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQVRQGLPGGRDGREWGLSRVLALHSSC

--------------------------------------------------------------

>52152_52152_6_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887704_ENST00000292385_length(amino acids)=411AA_BP=19
MNSGVAMKYGNDSSAELSEGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRM
APTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPA
EDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAE
VPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYF

--------------------------------------------------------------

>52152_52152_7_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887704_ENST00000309007_length(amino acids)=411AA_BP=19
MNSGVAMKYGNDSSAELSEGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRM
APTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPA
EDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAE
VPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYF

--------------------------------------------------------------

>52152_52152_8_MCC-DBN1_MCC_chr5_112630026_ENST00000302475_DBN1_chr5_176887704_ENST00000393565_length(amino acids)=457AA_BP=19
MNSGVAMKYGNDSSAELSEGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGRPYCPFIKA
SDSGPSSSSSSSSSPPRTPFPYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPP
PAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADT
TVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGE
SLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDITCWDADPVPEEE

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:112630026/chr5:176887704)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MCC Q9HCC0	DBN1 Q16643
FUNCTION: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism. {ECO:0000269\|PubMed:17360195}.	FUNCTION: Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed:20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed:20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity). {ECO:0000250\|UniProtKB:Q9QXS6, ECO:0000269\|PubMed:20215400}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MCC

chr5:112630026

chr5:176887556

ENST00000302475

766_782

19.0

830.0

Motif

Nuclear localization signal

Hgene

MCC

chr5:112630026

chr5:176887556

ENST00000302475

826_829

19.0

830.0

Motif

Note=PDZ-binding

Hgene

MCC

chr5:112630026

chr5:176887556

ENST00000408903

766_782

1020.0

Motif

Nuclear localization signal

Hgene

MCC

chr5:112630026

chr5:176887556

ENST00000408903

826_829

1020.0

Motif

Note=PDZ-binding

Hgene

MCC

chr5:112630026

chr5:176887704

ENST00000302475

766_782

19.0

830.0

Motif

Nuclear localization signal

Hgene

MCC

chr5:112630026

chr5:176887704

ENST00000302475

826_829

19.0

830.0

Motif

Note=PDZ-binding

Hgene

MCC

chr5:112630026

chr5:176887704

ENST00000408903

766_782

1020.0

Motif

Nuclear localization signal

Hgene

MCC

chr5:112630026

chr5:176887704

ENST00000408903

826_829

1020.0

Motif

Note=PDZ-binding

Tgene

DBN1

chr5:112630026

chr5:176887556

ENST00000292385

3_134

279.0

652.0

Domain

ADF-H

Tgene

DBN1

chr5:112630026

chr5:176887556

ENST00000309007

3_134

277.0

650.0

Domain

ADF-H

Tgene

DBN1

chr5:112630026

chr5:176887556

ENST00000393565

3_134

277.0

696.0

Domain

ADF-H

Tgene

DBN1

chr5:112630026

chr5:176887704

ENST00000292385

3_134

259.0

652.0

Domain

ADF-H

Tgene

DBN1

chr5:112630026

chr5:176887704

ENST00000309007

3_134

257.0

650.0

Domain

ADF-H

Tgene

DBN1

chr5:112630026

chr5:176887704

ENST00000393565

3_134

257.0

696.0

Domain

ADF-H

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MCC
DBN1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to MCC-DBN1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to MCC-DBN1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source