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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MET-CAPZA2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MET-CAPZA2
	FusionPDB ID: 53019
	FusionGDB2.0 ID: 53019
		Hgene	Tgene
	Gene symbol	MET	CAPZA2
	Gene ID	8731	830
	Gene name	RNA guanine-7 methyltransferase	capping actin protein of muscle Z-line subunit alpha 2
	Synonyms	CMT1\|CMT1c\|MET\|Met\|RG7MT1\|cm1p\|hCMT1\|hMet	CAPPA2\|CAPZ
	Cytomap	18p11.21	7q31.2
	Type of gene	protein-coding	protein-coding
	Description	mRNA cap guanine-N7 methyltransferaseRNA (guanine-7-) methyltransferasehcm1pmRNA (guanine-7-)methyltransferasemRNA (guanine-N(7)-)-methyltransferasemRNA cap methyltransferase	F-actin-capping protein subunit alpha-2F-actin capping protein alpha-2 subunitcapZ alpha-2capping actin protein of muscle Z-line alpha subunit 2capping protein (actin filament) muscle Z-line, alpha 2epididymis secretory sperm binding protein
	Modification date	20200329	20200313
	UniProtAcc	Q9H1A3	P47755
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000318493, ENST00000397752, ENST00000436117, ENST00000495962, ENST00000539704,	ENST00000466023, ENST00000361183, ENST00000458284, ENST00000490693,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	11 X 10 X 9=990	8 X 5 X 7=280
	# samples	16	8
	** MAII score	log2(16/990*10)=-2.62935662007961 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/280*10)=-1.8073549220576 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MET [Title/Abstract] AND CAPZA2 [Title/Abstract] AND fusion [Title/Abstract] Engineering and Functional Characterization of Fusion Genes Identifies Novel Oncogenic Drivers of Cancer (pmid: 28512244)
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	CAPZA2(116550365)-MET(116409699), # samples:2 CAPZA2(116528244)-MET(116395409), # samples:2 CAPZA2(116528244)-MET(116414935), # samples:2 MET(116399544)-CAPZA2(116528181), # samples:2
Anticipated loss of major functional domain due to fusion event.	CAPZA2-MET seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CAPZA2-MET seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MET-CAPZA2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MET-CAPZA2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MET-CAPZA2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MET-CAPZA2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CAPZA2-MET seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. CAPZA2-MET seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. CAPZA2-MET seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CAPZA2-MET seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MET	GO:0006370	7-methylguanosine mRNA capping	27422871

Fusion gene breakpoints across MET (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CAPZA2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	UCEC	TCGA-BK-A139-01A	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+
ChimerDB4	UCEC	TCGA-BK-A139-02A	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000397752	MET	chr7	116399544	+	ENST00000361183	CAPZA2	chr7	116528181	+	7568	2564	200	3385	1061
ENST00000397752	MET	chr7	116399544	+	ENST00000458284	CAPZA2	chr7	116528181	+	3895	2564	200	3043	947
ENST00000397752	MET	chr7	116399544	+	ENST00000490693	CAPZA2	chr7	116528181	+	3322	2564	200	2965	921
ENST00000318493	MET	chr7	116399544	+	ENST00000361183	CAPZA2	chr7	116528181	+	7609	2605	187	3426	1079
ENST00000318493	MET	chr7	116399544	+	ENST00000458284	CAPZA2	chr7	116528181	+	3936	2605	187	3084	965
ENST00000318493	MET	chr7	116399544	+	ENST00000490693	CAPZA2	chr7	116528181	+	3363	2605	187	3006	939

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000397752	ENST00000361183	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+	8.52E-05	0.99991477
ENST00000397752	ENST00000458284	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+	0.000121268	0.99987876
ENST00000397752	ENST00000490693	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+	0.000137402	0.99986255
ENST00000318493	ENST00000361183	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+	0.000217308	0.99978274
ENST00000318493	ENST00000458284	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+	0.000106795	0.9998932
ENST00000318493	ENST00000490693	MET	chr7	116399544	+	CAPZA2	chr7	116528181	+	0.000113002	0.999887

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>53019_53019_1_MET-CAPZA2_MET_chr7_116399544_ENST00000318493_CAPZA2_chr7_116528181_ENST00000361183_length(amino acids)=1079AA_BP=0
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL
EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL
GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD
AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS
AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL
TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW
CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM
NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF
AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISTWWKEPLNIVSFLFCFASGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIA
AKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATD
PRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVH

--------------------------------------------------------------

>53019_53019_2_MET-CAPZA2_MET_chr7_116399544_ENST00000318493_CAPZA2_chr7_116528181_ENST00000458284_length(amino acids)=965AA_BP=0
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL
EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL
GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD
AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS
AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL
TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW
CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM
NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF
AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISTWWKEPLNIVSFLFCFASGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIA
AKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATD

--------------------------------------------------------------

>53019_53019_3_MET-CAPZA2_MET_chr7_116399544_ENST00000318493_CAPZA2_chr7_116528181_ENST00000490693_length(amino acids)=939AA_BP=0
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL
EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL
GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD
AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS
AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL
TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW
CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM
NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF
AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISTWWKEPLNIVSFLFCFASGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIA
AKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATD

--------------------------------------------------------------

>53019_53019_4_MET-CAPZA2_MET_chr7_116399544_ENST00000397752_CAPZA2_chr7_116528181_ENST00000361183_length(amino acids)=1061AA_BP=0
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL
EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL
GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD
AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS
AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL
TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW
CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM
NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF
AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIAAKFIIHAPPGEFNEVFND
VRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVE
TALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDS

--------------------------------------------------------------

>53019_53019_5_MET-CAPZA2_MET_chr7_116399544_ENST00000397752_CAPZA2_chr7_116528181_ENST00000458284_length(amino acids)=947AA_BP=0
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL
EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL
GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD
AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS
AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL
TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW
CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM
NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF
AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIAAKFIIHAPPGEFNEVFND
VRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVE

--------------------------------------------------------------

>53019_53019_6_MET-CAPZA2_MET_chr7_116399544_ENST00000397752_CAPZA2_chr7_116528181_ENST00000490693_length(amino acids)=921AA_BP=0
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL
EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL
GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD
AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS
AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL
TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW
CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM
NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF
AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIAAKFIIHAPPGEFNEVFND
VRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVE

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:116550365/chr7:116409699)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MET Q9H1A3	CAPZA2 P47755
	FUNCTION: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

27_515

806.0

1409.0

Domain

Sema

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

563_655

806.0

1409.0

Domain

Note=IPT/TIG 1

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

657_739

806.0

1409.0

Domain

Note=IPT/TIG 2

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

27_515

788.0

1391.0

Domain

Sema

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

563_655

788.0

1391.0

Domain

Note=IPT/TIG 1

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

657_739

788.0

1391.0

Domain

Note=IPT/TIG 2

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

1078_1345

806.0

1409.0

Domain

Protein kinase

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

742_836

806.0

1409.0

Domain

Note=IPT/TIG 3

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

1078_1345

788.0

1391.0

Domain

Protein kinase

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

742_836

788.0

1391.0

Domain

Note=IPT/TIG 3

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

1078_1345

0.0

765.0

Domain

Protein kinase

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

27_515

0.0

765.0

Domain

Sema

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

563_655

0.0

765.0

Domain

Note=IPT/TIG 1

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

657_739

0.0

765.0

Domain

Note=IPT/TIG 2

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

742_836

0.0

765.0

Domain

Note=IPT/TIG 3

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

1084_1092

806.0

1409.0

Nucleotide binding

ATP

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

1084_1092

788.0

1391.0

Nucleotide binding

ATP

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

1084_1092

0.0

765.0

Nucleotide binding

ATP

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

25_932

806.0

1409.0

Topological domain

Extracellular

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

956_1390

806.0

1409.0

Topological domain

Cytoplasmic

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

25_932

788.0

1391.0

Topological domain

Extracellular

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

956_1390

788.0

1391.0

Topological domain

Cytoplasmic

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

25_932

0.0

765.0

Topological domain

Extracellular

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

956_1390

0.0

765.0

Topological domain

Cytoplasmic

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000318493

933_955

806.0

1409.0

Transmembrane

Helical

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000397752

933_955

788.0

1391.0

Transmembrane

Helical

Hgene

MET

chr7:116399544

chr7:116528181

ENST00000436117

933_955

0.0

765.0

Transmembrane

Helical

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1848_MET_116399544_CAPZA2_116528181_ranked_0.pdb	MET	116399544	116399544	ENST00000490693	CAPZA2	chr7	116528181	+	MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVL EHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSAL GAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLD AQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRS AMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDL TIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGW CHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAM NKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEF AVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISTWWKEPLNIVSFLFCFASGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVVRIA AKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATD PRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVH	1079

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

MET_pLDDT.png

CAPZA2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MET
CAPZA2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	MET	chr7:116399544	chr7:116528181	ENST00000318493	+	10	21	1320_1359	806.0	1409.0	MUC20
Hgene	MET	chr7:116399544	chr7:116528181	ENST00000397752	+	10	21	1320_1359	788.0	1391.0	MUC20
Hgene	MET	chr7:116399544	chr7:116528181	ENST00000436117	+	1	9	1320_1359	0.0	765.0	MUC20
Hgene	MET	chr7:116399544	chr7:116528181	ENST00000318493	+	10	21	1212_1390	806.0	1409.0	RANBP9
Hgene	MET	chr7:116399544	chr7:116528181	ENST00000397752	+	10	21	1212_1390	788.0	1391.0	RANBP9
Hgene	MET	chr7:116399544	chr7:116528181	ENST00000436117	+	1	9	1212_1390	0.0	765.0	RANBP9

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Related Drugs to MET-CAPZA2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to MET-CAPZA2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source