| UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY |
|
|||||||
|
Fusion Protein:MIB1-HLTF |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: MIB1-HLTF | FusionPDB ID: 53489 | FusionGDB2.0 ID: 53489 | Hgene | Tgene | Gene symbol | MIB1 | HLTF | Gene ID | 57534 | 6596 |
| Gene name | mindbomb E3 ubiquitin protein ligase 1 | helicase like transcription factor | |
| Synonyms | DIP-1|DIP1|LVNC7|MIB|ZZANK2|ZZZ6 | HIP116|HIP116A|HLTF1|RNF80|SMARCA3|SNF2L3|ZBU1 | |
| Cytomap | 18q11.2 | 3q24 | |
| Type of gene | protein-coding | protein-coding | |
| Description | E3 ubiquitin-protein ligase MIB1DAPK-interacting protein 1RING-type E3 ubiquitin transferase MIB1ubiquitin ligase mind bombzinc finger ZZ type with ankyrin repeat domain protein 2 | helicase-like transcription factorDNA-binding protein/plasminogen activator inhibitor-1 regulatorRING finger protein 80RING-type E3 ubiquitin transferase HLTFSNF2-like 3SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfa | |
| Modification date | 20200313 | 20200322 | |
| UniProtAcc | Q86YT6 | Q14527 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000578646, ENST00000261537, | ENST00000310053, ENST00000392912, ENST00000465259, ENST00000494055, ENST00000481663, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 11 X 12 X 7=924 | 5 X 5 X 3=75 |
| # samples | 14 | 5 | |
| ** MAII score | log2(14/924*10)=-2.72246602447109 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(5/75*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: MIB1 [Title/Abstract] AND HLTF [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | MIB1(19353689)-HLTF(148802676), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | MIB1-HLTF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MIB1-HLTF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MIB1-HLTF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MIB1-HLTF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MIB1-HLTF seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. MIB1-HLTF seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | MIB1 | GO:0006511 | ubiquitin-dependent protein catabolic process | 23615451 |
| Fusion gene breakpoints across MIB1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
| Fusion gene breakpoints across HLTF (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
Top |
Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | SARC | TCGA-SI-A71Q-01A | MIB1 | chr18 | 19353689 | - | HLTF | chr3 | 148802676 | - |
Top |
Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000261537 | MIB1 | chr18 | 19353689 | - | ENST00000392912 | HLTF | chr3 | 148802676 | - | 4608 | 900 | 844 | 3909 | 1021 |
| ENST00000261537 | MIB1 | chr18 | 19353689 | - | ENST00000494055 | HLTF | chr3 | 148802676 | - | 4295 | 900 | 844 | 3909 | 1021 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000261537 | ENST00000392912 | MIB1 | chr18 | 19353689 | - | HLTF | chr3 | 148802676 | - | 0.00036007 | 0.99963987 |
| ENST00000261537 | ENST00000494055 | MIB1 | chr18 | 19353689 | - | HLTF | chr3 | 148802676 | - | 0.000387855 | 0.99961215 |
Top |
Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >53489_53489_1_MIB1-HLTF_MIB1_chr18_19353689_ENST00000261537_HLTF_chr3_148802676_ENST00000392912_length(amino acids)=1021AA_BP=19 MSSGIMVLRTFTELALRAWDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVN NNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQLKKHG FKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSK ELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSE KADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDV AFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVL TTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTI QRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGL LLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCP LCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQK KRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKR -------------------------------------------------------------- >53489_53489_2_MIB1-HLTF_MIB1_chr18_19353689_ENST00000261537_HLTF_chr3_148802676_ENST00000494055_length(amino acids)=1021AA_BP=19 MSSGIMVLRTFTELALRAWDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVN NNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQLKKHG FKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSK ELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSE KADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDV AFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVL TTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTI QRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGL LLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCP LCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQK KRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKR -------------------------------------------------------------- |
Top |
Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr18:19353689/chr3:148802676) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
 |
| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| MIB1 | HLTF |
| FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. {ECO:0000250, ECO:0000269|PubMed:24121310}. | FUNCTION: Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. {ECO:0000250, ECO:0000269|PubMed:10391891, ECO:0000269|PubMed:18316726, ECO:0000269|PubMed:18719106, ECO:0000269|PubMed:7876228, ECO:0000269|PubMed:8672239, ECO:0000269|PubMed:9126292}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 6_74 | 212.0 | 1007.0 | Domain | MIB/HERC2 1 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 79_126 | 212.0 | 1007.0 | Zinc finger | ZZ-type |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000310053 | 0 | 25 | 38_287 | 6.666666666666667 | 1010.0 | DNA binding | . | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000392912 | 0 | 26 | 38_287 | 6.666666666666667 | 1161.6666666666667 | DNA binding | . | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000465259 | 0 | 25 | 38_287 | 6.666666666666667 | 1009.0 | DNA binding | . | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000494055 | 0 | 26 | 38_287 | 6.666666666666667 | 1040.6666666666667 | DNA binding | . | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000310053 | 0 | 25 | 435_606 | 6.666666666666667 | 1010.0 | Domain | Helicase ATP-binding | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000310053 | 0 | 25 | 837_996 | 6.666666666666667 | 1010.0 | Domain | Helicase C-terminal | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000392912 | 0 | 26 | 435_606 | 6.666666666666667 | 1161.6666666666667 | Domain | Helicase ATP-binding | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000392912 | 0 | 26 | 837_996 | 6.666666666666667 | 1161.6666666666667 | Domain | Helicase C-terminal | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000465259 | 0 | 25 | 435_606 | 6.666666666666667 | 1009.0 | Domain | Helicase ATP-binding | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000465259 | 0 | 25 | 837_996 | 6.666666666666667 | 1009.0 | Domain | Helicase C-terminal | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000494055 | 0 | 26 | 435_606 | 6.666666666666667 | 1040.6666666666667 | Domain | Helicase ATP-binding | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000494055 | 0 | 26 | 837_996 | 6.666666666666667 | 1040.6666666666667 | Domain | Helicase C-terminal | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000310053 | 0 | 25 | 557_560 | 6.666666666666667 | 1010.0 | Motif | Note=DEGH box | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000392912 | 0 | 26 | 557_560 | 6.666666666666667 | 1161.6666666666667 | Motif | Note=DEGH box | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000465259 | 0 | 25 | 557_560 | 6.666666666666667 | 1009.0 | Motif | Note=DEGH box | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000494055 | 0 | 26 | 557_560 | 6.666666666666667 | 1040.6666666666667 | Motif | Note=DEGH box | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000310053 | 0 | 25 | 294_301 | 6.666666666666667 | 1010.0 | Nucleotide binding | ATP | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000392912 | 0 | 26 | 294_301 | 6.666666666666667 | 1161.6666666666667 | Nucleotide binding | ATP | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000465259 | 0 | 25 | 294_301 | 6.666666666666667 | 1009.0 | Nucleotide binding | ATP | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000494055 | 0 | 26 | 294_301 | 6.666666666666667 | 1040.6666666666667 | Nucleotide binding | ATP | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000310053 | 0 | 25 | 760_801 | 6.666666666666667 | 1010.0 | Zinc finger | RING-type | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000392912 | 0 | 26 | 760_801 | 6.666666666666667 | 1161.6666666666667 | Zinc finger | RING-type | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000465259 | 0 | 25 | 760_801 | 6.666666666666667 | 1009.0 | Zinc finger | RING-type | |
| Tgene | HLTF | chr18:19353689 | chr3:148802676 | ENST00000494055 | 0 | 26 | 760_801 | 6.666666666666667 | 1040.6666666666667 | Zinc finger | RING-type |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 935_962 | 212.0 | 1007.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 143_221 | 212.0 | 1007.0 | Domain | MIB/HERC2 2 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 430_460 | 212.0 | 1007.0 | Repeat | Note=ANK 1 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 463_492 | 212.0 | 1007.0 | Repeat | Note=ANK 2 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 496_525 | 212.0 | 1007.0 | Repeat | Note=ANK 3 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 529_558 | 212.0 | 1007.0 | Repeat | Note=ANK 4 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 562_591 | 212.0 | 1007.0 | Repeat | Note=ANK 5 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 595_627 | 212.0 | 1007.0 | Repeat | Note=ANK 6 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 631_661 | 212.0 | 1007.0 | Repeat | Note=ANK 7 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 665_694 | 212.0 | 1007.0 | Repeat | Note=ANK 8 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 698_729 | 212.0 | 1007.0 | Repeat | Note=ANK 9 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 819_854 | 212.0 | 1007.0 | Zinc finger | RING-type 1 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 866_901 | 212.0 | 1007.0 | Zinc finger | RING-type 2 |
| Hgene | MIB1 | chr18:19353689 | chr3:148802676 | ENST00000261537 | - | 4 | 21 | 963_996 | 212.0 | 1007.0 | Zinc finger | RING-type 3 |
Top |
Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| MIB1 | |
| HLTF |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
Top |
Related Drugs to MIB1-HLTF |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
Top |
Related Diseases to MIB1-HLTF |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Copyright 2021-Present -The University of Texas Health Science Center at Houston (UTHealth)
Web File Viewing | Emergency Information |Campus Carry|Site Policies