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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MINK1-USO1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MINK1-USO1
	FusionPDB ID: 53696
	FusionGDB2.0 ID: 53696
		Hgene	Tgene
	Gene symbol	MINK1	USO1
	Gene ID	50488	8615
	Gene name	misshapen like kinase 1	USO1 vesicle transport factor
	Synonyms	B55\|MAP4K6\|MINK\|YSK2\|ZC3	P115\|TAP\|VDP
	Cytomap	17p13.2	4q21.1
	Type of gene	protein-coding	protein-coding
	Description	misshapen-like kinase 1GCK family kinase MINKMAPK/ERK kinase kinase kinase 6MEK kinase kinase 6MEKKK 6misshapen/NIK-related kinasemitogen-activated protein kinase kinase kinase kinase 6	general vesicular transport factor p115USO1 vesicle docking protein homologtranscytosis associated proteinvesicle docking protein p115
	Modification date	20200320	20200313
	UniProtAcc	Q8N4C8	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000347992, ENST00000355280, ENST00000453408,	ENST00000514213, ENST00000538159,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	13 X 8 X 5=520	4 X 5 X 3=60
	# samples	17	5
	** MAII score	log2(17/520*10)=-1.61297687689075 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(5/60*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MINK1 [Title/Abstract] AND USO1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MINK1(4762623)-USO1(76720775), # samples:1 MINK1(4736935)-USO1(76720775), # samples:1
Anticipated loss of major functional domain due to fusion event.	MINK1-USO1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MINK1-USO1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MINK1	GO:0046777	protein autophosphorylation	15469942

Fusion gene breakpoints across MINK1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across USO1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUAD	TCGA-38-4632-01A	MINK1	chr17	4736935	-	USO1	chr4	76720775	+
ChimerDB4	LUAD	TCGA-38-4632-01A	MINK1	chr17	4762623	+	USO1	chr4	76720775	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000355280	MINK1	chr17	4736935	-	ENST00000538159	USO1	chr4	76720775	+	1690	253	196	1689	498
ENST00000355280	MINK1	chr17	4736935	-	ENST00000514213	USO1	chr4	76720775	+	1690	253	196	1689	498
ENST00000347992	MINK1	chr17	4736935	-	ENST00000538159	USO1	chr4	76720775	+	1678	241	184	1677	498
ENST00000347992	MINK1	chr17	4736935	-	ENST00000514213	USO1	chr4	76720775	+	1678	241	184	1677	498
ENST00000453408	MINK1	chr17	4736935	-	ENST00000538159	USO1	chr4	76720775	+	1494	57	0	1493	497
ENST00000453408	MINK1	chr17	4736935	-	ENST00000514213	USO1	chr4	76720775	+	1494	57	0	1493	497

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000355280	ENST00000538159	MINK1	chr17	4736935	-	USO1	chr4	76720775	+	0.004097383	0.9959026
ENST00000355280	ENST00000514213	MINK1	chr17	4736935	-	USO1	chr4	76720775	+	0.004097383	0.9959026
ENST00000347992	ENST00000538159	MINK1	chr17	4736935	-	USO1	chr4	76720775	+	0.004056113	0.99594384
ENST00000347992	ENST00000514213	MINK1	chr17	4736935	-	USO1	chr4	76720775	+	0.004056113	0.99594384
ENST00000453408	ENST00000538159	MINK1	chr17	4736935	-	USO1	chr4	76720775	+	0.005910256	0.9940897
ENST00000453408	ENST00000514213	MINK1	chr17	4736935	-	USO1	chr4	76720775	+	0.005910256	0.9940897

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>53696_53696_1_MINK1-USO1_MINK1_chr17_4736935_ENST00000347992_USO1_chr4_76720775_ENST00000514213_length(amino acids)=498AA_BP=17
MGDPAPARSLDDIDLSALRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNS
LESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTL
EQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRL
REEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAF
AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD

--------------------------------------------------------------

>53696_53696_2_MINK1-USO1_MINK1_chr17_4736935_ENST00000347992_USO1_chr4_76720775_ENST00000538159_length(amino acids)=498AA_BP=17
MGDPAPARSLDDIDLSALRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNS
LESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTL
EQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRL
REEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAF
AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD

--------------------------------------------------------------

>53696_53696_3_MINK1-USO1_MINK1_chr17_4736935_ENST00000355280_USO1_chr4_76720775_ENST00000514213_length(amino acids)=498AA_BP=17
MGDPAPARSLDDIDLSALRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNS
LESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTL
EQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRL
REEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAF
AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD

--------------------------------------------------------------

>53696_53696_4_MINK1-USO1_MINK1_chr17_4736935_ENST00000355280_USO1_chr4_76720775_ENST00000538159_length(amino acids)=498AA_BP=17
MGDPAPARSLDDIDLSALRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNS
LESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTL
EQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRL
REEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAF
AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD

--------------------------------------------------------------

>53696_53696_5_MINK1-USO1_MINK1_chr17_4736935_ENST00000453408_USO1_chr4_76720775_ENST00000514213_length(amino acids)=497AA_BP=17
MGDPAPARSLDDIDLSALRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNS
LESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTL
EQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRL
REEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAF
AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD

--------------------------------------------------------------

>53696_53696_6_MINK1-USO1_MINK1_chr17_4736935_ENST00000453408_USO1_chr4_76720775_ENST00000538159_length(amino acids)=497AA_BP=17
MGDPAPARSLDDIDLSALRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNS
LESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTL
EQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRL
REEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAF
AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLAD

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:4762623/chr4:76720775)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MINK1 Q8N4C8	.
FUNCTION: Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.; FUNCTION: Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000347992

359_495

19.0

1304.0

Compositional bias

Note=Gln-rich

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000347992

542_729

19.0

1304.0

Compositional bias

Note=Pro-rich

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000355280

359_495

19.0

1333.0

Compositional bias

Note=Gln-rich

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000355280

542_729

19.0

1333.0

Compositional bias

Note=Pro-rich

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000453408

359_495

19.0

1313.0

Compositional bias

Note=Gln-rich

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000453408

542_729

19.0

1313.0

Compositional bias

Note=Pro-rich

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000347992

1019_1306

19.0

1304.0

Domain

CNH

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000347992

25_289

19.0

1304.0

Domain

Protein kinase

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000355280

1019_1306

19.0

1333.0

Domain

CNH

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000355280

25_289

19.0

1333.0

Domain

Protein kinase

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000453408

1019_1306

19.0

1313.0

Domain

CNH

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000453408

25_289

19.0

1313.0

Domain

Protein kinase

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000347992

31_39

19.0

1304.0

Nucleotide binding

ATP

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000355280

31_39

19.0

1333.0

Nucleotide binding

ATP

Hgene

MINK1

chr17:4736935

chr4:76720775

ENST00000453408

31_39

19.0

1313.0

Nucleotide binding

ATP

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MINK1
USO1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to MINK1-USO1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to MINK1-USO1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source