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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:APC-DTWD2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: APC-DTWD2
	FusionPDB ID: 5380
	FusionGDB2.0 ID: 5380
		Hgene	Tgene
	Gene symbol	APC	DTWD2
	Gene ID	5624	285605
	Gene name	protein C, inactivator of coagulation factors Va and VIIIa	DTW domain containing 2
	Synonyms	APC\|PC\|PROC1\|THPH3\|THPH4	-
	Cytomap	2q14.3	5q23.1
	Type of gene	protein-coding	protein-coding
	Description	vitamin K-dependent protein CProtein C-Nagoyaactivated protein Canticoagulant protein Cautoprothrombin IIAblood coagulation factor XIVprepro-protein Ctype I protein C	DTW domain-containing protein 2
	Modification date	20200313	20200313
	UniProtAcc	P02743	Q8NBA8
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000257430, ENST00000457016, ENST00000508376, ENST00000505350,	ENST00000304058, ENST00000510708, ENST00000515439,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	14 X 10 X 8=1120	9 X 7 X 5=315
	# samples	14	10
	** MAII score	log2(14/1120*10)=-3 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(10/315*10)=-1.65535182861255 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: APC [Title/Abstract] AND DTWD2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	APC(112164669)-DTWD2(118183913), # samples:3
Anticipated loss of major functional domain due to fusion event.	APC-DTWD2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. APC-DTWD2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across APC (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across DTWD2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-EQ-A4SO-01A	APC	chr5	112164669	+	DTWD2	chr5	118183913	-
ChimerDB4	STAD	TCGA-EQ-A4SO	APC	chr5	112164669	+	DTWD2	chr5	118183913	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000457016	APC	chr5	112164669	+	ENST00000304058	DTWD2	chr5	118183913	-	6018	2123	380	2422	680
ENST00000457016	APC	chr5	112164669	+	ENST00000510708	DTWD2	chr5	118183913	-	3799	2123	380	2422	680
ENST00000457016	APC	chr5	112164669	+	ENST00000515439	DTWD2	chr5	118183913	-	3061	2123	380	2422	680
ENST00000257430	APC	chr5	112164669	+	ENST00000304058	DTWD2	chr5	118183913	-	5694	1799	56	2098	680
ENST00000257430	APC	chr5	112164669	+	ENST00000510708	DTWD2	chr5	118183913	-	3475	1799	56	2098	680
ENST00000257430	APC	chr5	112164669	+	ENST00000515439	DTWD2	chr5	118183913	-	2737	1799	56	2098	680
ENST00000508376	APC	chr5	112164669	+	ENST00000304058	DTWD2	chr5	118183913	-	5795	1900	157	2199	680
ENST00000508376	APC	chr5	112164669	+	ENST00000510708	DTWD2	chr5	118183913	-	3576	1900	157	2199	680
ENST00000508376	APC	chr5	112164669	+	ENST00000515439	DTWD2	chr5	118183913	-	2838	1900	157	2199	680

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000457016	ENST00000304058	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.000316756	0.99968326
ENST00000457016	ENST00000510708	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.000786154	0.9992138
ENST00000457016	ENST00000515439	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.002060664	0.9979393
ENST00000257430	ENST00000304058	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.000285115	0.9997149
ENST00000257430	ENST00000510708	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.000601048	0.99939895
ENST00000257430	ENST00000515439	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.001391467	0.9986085
ENST00000508376	ENST00000304058	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.000302792	0.9996972
ENST00000508376	ENST00000510708	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.000658958	0.9993411
ENST00000508376	ENST00000515439	APC	chr5	112164669	+	DTWD2	chr5	118183913	-	0.001543182	0.99845684

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>5380_5380_1_APC-DTWD2_APC_chr5_112164669_ENST00000257430_DTWD2_chr5_118183913_ENST00000304058_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_2_APC-DTWD2_APC_chr5_112164669_ENST00000257430_DTWD2_chr5_118183913_ENST00000510708_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_3_APC-DTWD2_APC_chr5_112164669_ENST00000257430_DTWD2_chr5_118183913_ENST00000515439_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_4_APC-DTWD2_APC_chr5_112164669_ENST00000457016_DTWD2_chr5_118183913_ENST00000304058_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_5_APC-DTWD2_APC_chr5_112164669_ENST00000457016_DTWD2_chr5_118183913_ENST00000510708_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_6_APC-DTWD2_APC_chr5_112164669_ENST00000457016_DTWD2_chr5_118183913_ENST00000515439_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_7_APC-DTWD2_APC_chr5_112164669_ENST00000508376_DTWD2_chr5_118183913_ENST00000304058_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_8_APC-DTWD2_APC_chr5_112164669_ENST00000508376_DTWD2_chr5_118183913_ENST00000510708_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

>5380_5380_9_APC-DTWD2_APC_chr5_112164669_ENST00000508376_DTWD2_chr5_118183913_ENST00000515439_length(amino acids)=680AA_BP=581
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK
MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL
QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN
MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL
HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV
CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL
QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:112164669/chr5:118183913)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
APC P02743	DTWD2 Q8NBA8
FUNCTION: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.	FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20a in the D-loop of several cytoplasmic tRNAs (acp3U(20a)) (PubMed:31804502). Also has a weak activity to form acp3U at position 20 in the D-loop of tRNAs (acp3U(20)) (PubMed:31804502). {ECO:0000269\|PubMed:31804502}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

127_248

581.0

2844.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

2_61

581.0

2844.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

127_248

581.0

2844.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

2_61

581.0

2844.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

127_248

581.0

2844.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

2_61

581.0

2844.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

453_495

581.0

2844.0

Repeat

Note=ARM 1

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

505_547

581.0

2844.0

Repeat

Note=ARM 2

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

453_495

581.0

2844.0

Repeat

Note=ARM 1

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

505_547

581.0

2844.0

Repeat

Note=ARM 2

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

453_495

581.0

2844.0

Repeat

Note=ARM 1

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

505_547

581.0

2844.0

Repeat

Note=ARM 2

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

1131_1156

581.0

2844.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

1558_1577

581.0

2844.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

1_730

581.0

2844.0

Compositional bias

Note=Leu-rich

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

731_2832

581.0

2844.0

Compositional bias

Note=Ser-rich

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

1131_1156

581.0

2844.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

1558_1577

581.0

2844.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

1_730

581.0

2844.0

Compositional bias

Note=Leu-rich

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

731_2832

581.0

2844.0

Compositional bias

Note=Ser-rich

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

1131_1156

581.0

2844.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

1558_1577

581.0

2844.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

1_730

581.0

2844.0

Compositional bias

Note=Leu-rich

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

731_2832

581.0

2844.0

Compositional bias

Note=Ser-rich

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

2803_2806

581.0

2844.0

Motif

Note=Microtubule tip localization signal

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

2841_2843

581.0

2844.0

Motif

Note=PDZ-binding

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

2803_2806

581.0

2844.0

Motif

Note=Microtubule tip localization signal

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

2841_2843

581.0

2844.0

Motif

Note=PDZ-binding

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

2803_2806

581.0

2844.0

Motif

Note=Microtubule tip localization signal

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

2841_2843

581.0

2844.0

Motif

Note=PDZ-binding

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

1866_1893

581.0

2844.0

Region

Note=Highly charged

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

960_1337

581.0

2844.0

Region

Note=Responsible for down-regulation through a process mediated by direct ubiquitination

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

1866_1893

581.0

2844.0

Region

Note=Highly charged

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

960_1337

581.0

2844.0

Region

Note=Responsible for down-regulation through a process mediated by direct ubiquitination

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

1866_1893

581.0

2844.0

Region

Note=Highly charged

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

960_1337

581.0

2844.0

Region

Note=Responsible for down-regulation through a process mediated by direct ubiquitination

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

548_591

581.0

2844.0

Repeat

Note=ARM 3

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

592_638

581.0

2844.0

Repeat

Note=ARM 4

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

639_683

581.0

2844.0

Repeat

Note=ARM 5

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

684_725

581.0

2844.0

Repeat

Note=ARM 6

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000257430

726_767

581.0

2844.0

Repeat

Note=ARM 7

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

548_591

581.0

2844.0

Repeat

Note=ARM 3

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

592_638

581.0

2844.0

Repeat

Note=ARM 4

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

639_683

581.0

2844.0

Repeat

Note=ARM 5

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

684_725

581.0

2844.0

Repeat

Note=ARM 6

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000457016

726_767

581.0

2844.0

Repeat

Note=ARM 7

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

548_591

581.0

2844.0

Repeat

Note=ARM 3

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

592_638

581.0

2844.0

Repeat

Note=ARM 4

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

639_683

581.0

2844.0

Repeat

Note=ARM 5

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

684_725

581.0

2844.0

Repeat

Note=ARM 6

Hgene

APC

chr5:112164669

chr5:118183913

ENST00000508376

726_767

581.0

2844.0

Repeat

Note=ARM 7

Tgene

DTWD2

chr5:112164669

chr5:118183913

ENST00000510708

178_181

199.0

299.0

Motif

Note=DXTW

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1400_APC_112164669_DTWD2_118183913_ranked_0.pdb	APC	112164669	112164669	ENST00000515439	DTWD2	chr5	118183913	-	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSK MSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSL QTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEIN MATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLL HGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAV CVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDL QQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKVQLKTSISSQYVIRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPL	680

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

APC_pLDDT.png

DTWD2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
APC
DTWD2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to APC-DTWD2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to APC-DTWD2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source