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Center for Computational Systems Medicine level3
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Potential Active Site Information

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Potentially Interacting Small Molecules through Virtual Screening

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Biochemical Features of Small Molecules with ADME

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Drug Toxicity Information

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:MKRN1-BRAF

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: MKRN1-BRAF
FusionPDB ID: 54112
FusionGDB2.0 ID: 54112
HgeneTgene
Gene symbol

MKRN1

BRAF

Gene ID

23608

673

Gene namemakorin ring finger protein 1B-Raf proto-oncogene, serine/threonine kinase
SynonymsRNF61B-RAF1|B-raf|BRAF1|NS7|RAFB1
Cytomap

7q34

7q34

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase makorin-1RING finger protein 61RING-type E3 ubiquitin transferase makorin-1serine/threonine-protein kinase B-raf94 kDa B-raf proteinB-Raf proto-oncogene serine/threonine-protein kinase (p94)B-Raf serine/threonine-proteinmurine sarcoma viral (v-raf) oncogene homolog B1proto-oncogene B-Rafv-raf murine sarcoma viral oncogene
Modification date2020031320200329
UniProtAcc

Q9UHC7

P15056

Ensembl transtripts involved in fusion geneENST idsENST00000255977, ENST00000443720, 
ENST00000474576, ENST00000437223, 
ENST00000480552, ENST00000481705, 
ENST00000288602, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score10 X 11 X 9=99048 X 58 X 16=44544
# samples 1569
** MAII scorelog2(15/990*10)=-2.72246602447109
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(69/44544*10)=-6.0124909441832
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: MKRN1 [Title/Abstract] AND BRAF [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)MKRN1(140158807)-BRAF(140487384), # samples:3
Anticipated loss of major functional domain due to fusion event.MKRN1-BRAF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
MKRN1-BRAF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
MKRN1-BRAF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
MKRN1-BRAF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMKRN1

GO:0000209

protein polyubiquitination

19536131

TgeneBRAF

GO:0000186

activation of MAPKK activity

29433126

TgeneBRAF

GO:0006468

protein phosphorylation

17563371

TgeneBRAF

GO:0010828

positive regulation of glucose transmembrane transport

23010278

TgeneBRAF

GO:0033138

positive regulation of peptidyl-serine phosphorylation

19667065

TgeneBRAF

GO:0043066

negative regulation of apoptotic process

19667065

TgeneBRAF

GO:0070374

positive regulation of ERK1 and ERK2 cascade

22065586

TgeneBRAF

GO:0071277

cellular response to calcium ion

18567582

TgeneBRAF

GO:0090150

establishment of protein localization to membrane

23010278


check buttonFusion gene breakpoints across MKRN1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across BRAF (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4HNSCTCGA-DQ-7590-01AMKRN1chr7

140158807

-BRAFchr7

140481493

-
ChimerDB4THCATCGA-J8-A3O1-01AMKRN1chr7

140158807

-BRAFchr7

140487384

-
ChimerDB4THCATCGA-J8-A3O1MKRN1chr7

140158806

-BRAFchr7

140487384

-
ChimerKB3..MKRN1chr7

140158806

-BRAFchr7

140481493

-
ChimerKB3..MKRN1chr7

140158807

-BRAFchr7

140487384

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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000255977MKRN1chr7140158806-ENST00000288602BRAFchr7140481493-21019962251982585
ENST00000474576MKRN1chr7140158806-ENST00000288602BRAFchr7140481493-18397341551720521
ENST00000443720MKRN1chr7140158806-ENST00000288602BRAFchr7140481493-20319261551912585
ENST00000255977MKRN1chr7140158807-ENST00000288602BRAFchr7140487384-22759962252156643
ENST00000474576MKRN1chr7140158807-ENST00000288602BRAFchr7140487384-20137341551894579
ENST00000443720MKRN1chr7140158807-ENST00000288602BRAFchr7140487384-22059261552086643
ENST00000255977MKRN1chr7140158807-ENST00000288602BRAFchr7140481493-21019962251982585
ENST00000474576MKRN1chr7140158807-ENST00000288602BRAFchr7140481493-18397341551720521
ENST00000443720MKRN1chr7140158807-ENST00000288602BRAFchr7140481493-20319261551912585
ENST00000255977MKRN1chr7140158807-ENST00000288602BRAFchr7140487384-22759962252156643
ENST00000474576MKRN1chr7140158807-ENST00000288602BRAFchr7140487384-20137341551894579
ENST00000443720MKRN1chr7140158807-ENST00000288602BRAFchr7140487384-22059261552086643
ENST00000255977MKRN1chr7140158806-ENST00000288602BRAFchr7140487384-22759962252156643
ENST00000474576MKRN1chr7140158806-ENST00000288602BRAFchr7140487384-20137341551894579
ENST00000443720MKRN1chr7140158806-ENST00000288602BRAFchr7140487384-22059261552086643

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000255977ENST00000288602MKRN1chr7140158807-BRAFchr7140481493-0.0070150290.99298495
ENST00000474576ENST00000288602MKRN1chr7140158807-BRAFchr7140481493-0.0044977240.9955023
ENST00000443720ENST00000288602MKRN1chr7140158807-BRAFchr7140481493-0.0074885760.9925114
ENST00000255977ENST00000288602MKRN1chr7140158807-BRAFchr7140487384-0.0070206490.99297935
ENST00000474576ENST00000288602MKRN1chr7140158807-BRAFchr7140487384-0.0064385420.9935615
ENST00000443720ENST00000288602MKRN1chr7140158807-BRAFchr7140487384-0.0076943330.9923057
ENST00000255977ENST00000288602MKRN1chr7140158806-BRAFchr7140487384-0.0070206490.99297935
ENST00000474576ENST00000288602MKRN1chr7140158806-BRAFchr7140487384-0.0064385420.9935615
ENST00000443720ENST00000288602MKRN1chr7140158806-BRAFchr7140487384-0.0076943330.9923057

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>54112_54112_1_MKRN1-BRAF_MKRN1_chr7_140158806_ENST00000255977_BRAF_chr7_140481493_ENST00000288602_length(amino acids)=585AA_BP=255
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKKTLGRRDSSDDWE
IPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHL
HIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKN
PYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSL

--------------------------------------------------------------

>54112_54112_2_MKRN1-BRAF_MKRN1_chr7_140158806_ENST00000255977_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=643AA_BP=257
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKDLIRDQGFRGDGG
STTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVK
MLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSII
HRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKT

--------------------------------------------------------------

>54112_54112_3_MKRN1-BRAF_MKRN1_chr7_140158806_ENST00000443720_BRAF_chr7_140481493_ENST00000288602_length(amino acids)=585AA_BP=255
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKKTLGRRDSSDDWE
IPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHL
HIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKN
PYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSL

--------------------------------------------------------------

>54112_54112_4_MKRN1-BRAF_MKRN1_chr7_140158806_ENST00000443720_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=643AA_BP=257
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKDLIRDQGFRGDGG
STTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVK
MLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSII
HRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKT

--------------------------------------------------------------

>54112_54112_5_MKRN1-BRAF_MKRN1_chr7_140158806_ENST00000474576_BRAF_chr7_140481493_ENST00000288602_length(amino acids)=521AA_BP=191
MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNS
NFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVL
HPMDAAQRSQHIKKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILL
FMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWS
GSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAE

--------------------------------------------------------------

>54112_54112_6_MKRN1-BRAF_MKRN1_chr7_140158806_ENST00000474576_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=579AA_BP=193
MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNS
NFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVL
HPMDAAQRSQHIKDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQI
TVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETK
FEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQS
DVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRS

--------------------------------------------------------------

>54112_54112_7_MKRN1-BRAF_MKRN1_chr7_140158807_ENST00000255977_BRAF_chr7_140481493_ENST00000288602_length(amino acids)=585AA_BP=255
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKKTLGRRDSSDDWE
IPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHL
HIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKN
PYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSL

--------------------------------------------------------------

>54112_54112_8_MKRN1-BRAF_MKRN1_chr7_140158807_ENST00000255977_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=643AA_BP=257
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKDLIRDQGFRGDGG
STTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVK
MLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSII
HRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKT

--------------------------------------------------------------

>54112_54112_9_MKRN1-BRAF_MKRN1_chr7_140158807_ENST00000443720_BRAF_chr7_140481493_ENST00000288602_length(amino acids)=585AA_BP=255
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKKTLGRRDSSDDWE
IPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHL
HIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKN
PYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSL

--------------------------------------------------------------

>54112_54112_10_MKRN1-BRAF_MKRN1_chr7_140158807_ENST00000443720_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=643AA_BP=257
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVC
KYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKDLIRDQGFRGDGG
STTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVK
MLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSII
HRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKT

--------------------------------------------------------------

>54112_54112_11_MKRN1-BRAF_MKRN1_chr7_140158807_ENST00000474576_BRAF_chr7_140481493_ENST00000288602_length(amino acids)=521AA_BP=191
MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNS
NFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVL
HPMDAAQRSQHIKKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILL
FMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWS
GSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAE

--------------------------------------------------------------

>54112_54112_12_MKRN1-BRAF_MKRN1_chr7_140158807_ENST00000474576_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=579AA_BP=193
MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNS
NFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVL
HPMDAAQRSQHIKDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQI
TVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETK
FEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQS
DVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRS

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:140158807/chr7:140487384)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
MKRN1

Q9UHC7

BRAF

P15056

FUNCTION: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. {ECO:0000269|PubMed:16785614, ECO:0000269|PubMed:19536131}.FUNCTION: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179). {ECO:0000269|PubMed:1508179, ECO:0000269|PubMed:21441910, ECO:0000269|PubMed:29433126, ECO:0000305}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneMKRN1chr7:140158806chr7:140487384ENST00000255977-48208_235257.0483.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158806chr7:140487384ENST00000255977-4855_82257.0483.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158806chr7:140487384ENST00000255977-4884_111257.0483.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158806chr7:140487384ENST00000443720-45208_235257.0330.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158806chr7:140487384ENST00000443720-4555_82257.0330.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158806chr7:140487384ENST00000443720-4584_111257.0330.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158806chr7:140487384ENST00000474576-4855_82193.0419.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158806chr7:140487384ENST00000474576-4884_111193.0419.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158807chr7:140481493ENST00000255977-48208_235257.0483.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158807chr7:140481493ENST00000255977-4855_82257.0483.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158807chr7:140481493ENST00000255977-4884_111257.0483.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158807chr7:140481493ENST00000443720-45208_235257.0330.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158807chr7:140481493ENST00000443720-4555_82257.0330.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158807chr7:140481493ENST00000443720-4584_111257.0330.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158807chr7:140481493ENST00000474576-4855_82193.0419.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158807chr7:140481493ENST00000474576-4884_111193.0419.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158807chr7:140487384ENST00000255977-48208_235257.0483.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158807chr7:140487384ENST00000255977-4855_82257.0483.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158807chr7:140487384ENST00000255977-4884_111257.0483.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158807chr7:140487384ENST00000443720-45208_235257.0330.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158807chr7:140487384ENST00000443720-4555_82257.0330.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158807chr7:140487384ENST00000443720-4584_111257.0330.0Zinc fingerC3H1-type 2
HgeneMKRN1chr7:140158807chr7:140487384ENST00000474576-4855_82193.0419.0Zinc fingerC3H1-type 1
HgeneMKRN1chr7:140158807chr7:140487384ENST00000474576-4884_111193.0419.0Zinc fingerC3H1-type 2
TgeneBRAFchr7:140158806chr7:140487384ENST00000288602718428_432380.0767.0Compositional biasNote=Poly-Ser
TgeneBRAFchr7:140158807chr7:140487384ENST00000288602718428_432380.0767.0Compositional biasNote=Poly-Ser
TgeneBRAFchr7:140158806chr7:140487384ENST00000288602718457_717380.0767.0DomainProtein kinase
TgeneBRAFchr7:140158807chr7:140481493ENST00000288602918457_717438.0767.0DomainProtein kinase
TgeneBRAFchr7:140158807chr7:140487384ENST00000288602718457_717380.0767.0DomainProtein kinase
TgeneBRAFchr7:140158806chr7:140487384ENST00000288602718463_471380.0767.0Nucleotide bindingATP
TgeneBRAFchr7:140158807chr7:140481493ENST00000288602918463_471438.0767.0Nucleotide bindingATP
TgeneBRAFchr7:140158807chr7:140487384ENST00000288602718463_471380.0767.0Nucleotide bindingATP

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneMKRN1chr7:140158806chr7:140487384ENST00000255977-48236_263257.0483.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158806chr7:140487384ENST00000443720-45236_263257.0330.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158806chr7:140487384ENST00000474576-48236_263193.0419.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158807chr7:140481493ENST00000255977-48236_263257.0483.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158807chr7:140481493ENST00000443720-45236_263257.0330.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158807chr7:140481493ENST00000474576-48236_263193.0419.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158807chr7:140487384ENST00000255977-48236_263257.0483.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158807chr7:140487384ENST00000443720-45236_263257.0330.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158807chr7:140487384ENST00000474576-48236_263193.0419.0RegionNote=Makorin-type Cys-His
HgeneMKRN1chr7:140158806chr7:140487384ENST00000255977-48281_335257.0483.0Zinc fingerRING-type
HgeneMKRN1chr7:140158806chr7:140487384ENST00000255977-48364_393257.0483.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158806chr7:140487384ENST00000443720-45281_335257.0330.0Zinc fingerRING-type
HgeneMKRN1chr7:140158806chr7:140487384ENST00000443720-45364_393257.0330.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158806chr7:140487384ENST00000474576-48208_235193.0419.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158806chr7:140487384ENST00000474576-48281_335193.0419.0Zinc fingerRING-type
HgeneMKRN1chr7:140158806chr7:140487384ENST00000474576-48364_393193.0419.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158807chr7:140481493ENST00000255977-48281_335257.0483.0Zinc fingerRING-type
HgeneMKRN1chr7:140158807chr7:140481493ENST00000255977-48364_393257.0483.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158807chr7:140481493ENST00000443720-45281_335257.0330.0Zinc fingerRING-type
HgeneMKRN1chr7:140158807chr7:140481493ENST00000443720-45364_393257.0330.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158807chr7:140481493ENST00000474576-48208_235193.0419.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158807chr7:140481493ENST00000474576-48281_335193.0419.0Zinc fingerRING-type
HgeneMKRN1chr7:140158807chr7:140481493ENST00000474576-48364_393193.0419.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158807chr7:140487384ENST00000255977-48281_335257.0483.0Zinc fingerRING-type
HgeneMKRN1chr7:140158807chr7:140487384ENST00000255977-48364_393257.0483.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158807chr7:140487384ENST00000443720-45281_335257.0330.0Zinc fingerRING-type
HgeneMKRN1chr7:140158807chr7:140487384ENST00000443720-45364_393257.0330.0Zinc fingerC3H1-type 4
HgeneMKRN1chr7:140158807chr7:140487384ENST00000474576-48208_235193.0419.0Zinc fingerC3H1-type 3
HgeneMKRN1chr7:140158807chr7:140487384ENST00000474576-48281_335193.0419.0Zinc fingerRING-type
HgeneMKRN1chr7:140158807chr7:140487384ENST00000474576-48364_393193.0419.0Zinc fingerC3H1-type 4
TgeneBRAFchr7:140158806chr7:140487384ENST00000288602718122_129380.0767.0Compositional biasNote=Poly-Ser
TgeneBRAFchr7:140158806chr7:140487384ENST000002886027186_11380.0767.0Compositional biasNote=Poly-Gly
TgeneBRAFchr7:140158807chr7:140481493ENST00000288602918122_129438.0767.0Compositional biasNote=Poly-Ser
TgeneBRAFchr7:140158807chr7:140481493ENST00000288602918428_432438.0767.0Compositional biasNote=Poly-Ser
TgeneBRAFchr7:140158807chr7:140481493ENST000002886029186_11438.0767.0Compositional biasNote=Poly-Gly
TgeneBRAFchr7:140158807chr7:140487384ENST00000288602718122_129380.0767.0Compositional biasNote=Poly-Ser
TgeneBRAFchr7:140158807chr7:140487384ENST000002886027186_11380.0767.0Compositional biasNote=Poly-Gly
TgeneBRAFchr7:140158806chr7:140487384ENST00000288602718155_227380.0767.0DomainRBD
TgeneBRAFchr7:140158807chr7:140481493ENST00000288602918155_227438.0767.0DomainRBD
TgeneBRAFchr7:140158807chr7:140487384ENST00000288602718155_227380.0767.0DomainRBD
TgeneBRAFchr7:140158806chr7:140487384ENST00000288602718234_280380.0767.0Zinc fingerPhorbol-ester/DAG-type
TgeneBRAFchr7:140158807chr7:140481493ENST00000288602918234_280438.0767.0Zinc fingerPhorbol-ester/DAG-type
TgeneBRAFchr7:140158807chr7:140487384ENST00000288602718234_280380.0767.0Zinc fingerPhorbol-ester/DAG-type


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file (338) >>>338.pdbFusion protein BP residue: 191
CIF file (338) >>>338.cif		MKRN1chr7140158806-BRAFchr7140481493-
MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQ
EEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSE
DWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLC
PYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKKTLGRRD
SSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQL
QAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIE
TKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDF
GLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIV
LYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAE
CLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSL
521
3D view using mol* of 338 (AA BP:191)
PDB file (444) >>>444.pdbFusion protein BP residue: 193
CIF file (444) >>>444.cif		MKRN1chr7140158807-BRAFchr7140487384-
MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQ
EEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSE
DWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLC
PYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKDLIRDQG
FRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNR
MKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNV
TAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSL
YHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHED
LTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQS
DVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPK
AMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAG
579
3D view using mol* of 444 (AA BP:193)
PDB file (449) >>>449.pdbFusion protein BP residue: 255
CIF file (449) >>>449.cif		MKRN1chr7140158806-BRAFchr7140481493-
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDG
SGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIY
GDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAE
SRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEES
EKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAA
QRSQHIKKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVA
VKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQW
CEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNN
IFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKN
PYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKV
RSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEP
585
3D view using mol* of 449 (AA BP:255)
PDB file (514) >>>514.pdbFusion protein BP residue: 257
CIF file (514) >>>514.cif		MKRN1chr7140158807-BRAFchr7140487384-
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDG
SGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIY
GDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAE
SRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEES
EKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAA
QRSQHIKDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQ
RERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYK
GKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKP
QLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSII
HRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPE
VIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGY
LSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPK
643
3D view using mol* of 514 (AA BP:257)


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
MKRN1_pLDDT.png
all structure
all structure
BRAF_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
MKRN1_BRAF_338_pLDDT.png (AA BP:191)
all structure
MKRN1_BRAF_338_pLDDT_and_active_sites.png (AA BP:191)
all structure
MKRN1_BRAF_338_violinplot.png (AA BP:191)
all structure
MKRN1_BRAF_444_pLDDT.png (AA BP:193)
all structure
MKRN1_BRAF_444_pLDDT_and_active_sites.png (AA BP:193)
all structure
MKRN1_BRAF_444_violinplot.png (AA BP:193)
all structure
MKRN1_BRAF_449_pLDDT.png (AA BP:255)
all structure
MKRN1_BRAF_449_pLDDT_and_active_sites.png (AA BP:255)
all structure
MKRN1_BRAF_449_violinplot.png (AA BP:255)
all structure
MKRN1_BRAF_514_pLDDT.png (AA BP:257)
all structure
MKRN1_BRAF_514_pLDDT_and_active_sites.png (AA BP:257)
all structure
MKRN1_BRAF_514_violinplot.png (AA BP:257)
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots
MKRN1_BRAF_338.png
all structure
MKRN1_BRAF_444.png
all structure
MKRN1_BRAF_449.png
all structure
MKRN1_BRAF_514.png
all structure

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Potential Active Site Information


check button The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.
Fusion AA seq ID in FusionPDBSite scoreSizeD scoreVolumeExposureEnclosureContactPhobicPhilicBalanceDon/AccResidues
3381.0363511.0841138.4170.5920.6920.8960.9770.8331.1730.843Chain A: 35,36,39,99,100,101,102,103,105,106,107,1
08,110,111,112,113,114,170,172,173,174,175,179,180
,181,182,188,191,192,194,195,196,198,199,200,201,2
02,204,205,251,254,255,257,258,259,261,262,263,264
,265,266,268,270,274,313,317,320,321,323,324,325,3
46,390,461,462,463,466,467,470
4441.0655571.0991295.5110.5250.7550.9640.8360.9040.9250.984Chain A: 33,100,101,102,104,105,108,109,110,111,11
3,152,153,156,157,158,159,160,161,168,169,170,171,
176,177,178,275,276,277,278,279,280,281,282,283,28
4,294,296,299,300,314,318,327,329,340,342,343,344,
345,347,348,349,351,387,388,389,390,391,393,394,39
6,406,407,408,409,410,411,412,414,415,416,417,418,
420,421,422,423,424,425,426,427,428,429,432,433,43
4,437,440,442,443,444,445,446,450,451,454,475
4491.085051.1211222.7950.4820.7610.9860.9960.8451.1790.942Chain A: 17,18,19,20,21,22,23,282,283,284,285,286,
287,288,290,300,302,304,305,306,313,324,333,335,34
6,348,349,350,351,352,353,354,355,357,358,393,394,
395,396,397,399,400,402,412,413,414,416,417,418,42
0,421,422,423,424,425,426,427,428,429,430,431,432,
433,434,435,438,439,440,443,446,448,449,450,451,45
2,456,457,460
5141.065041.081091.0830.4930.7740.9870.8250.9970.8271.058Chain A: 170,171,172,173,174,175,176,177,178,179,3
38,339,340,341,342,343,344,345,348,358,360,362,363
,382,391,393,404,406,407,408,409,410,411,412,413,4
15,416,420,452,453,454,455,457,458,460,461,462,470
,471,472,474,475,477,478,479,481,482,486,487,488,4
89,490,491,492,493,496,497,498,501,504,506,507,509
,510,514,515,518

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Potentially Interacting Small Molecules through Virtual Screening


check button The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.
Fusion AA seq ID in FusionPDBZINC IDDrugBank IDDrug nameDocking scoreGlide gscore

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check button Drug information from DrugBank of the top 20 interacting small molecules.
ZINC IDDrugBank IDDrug nameDrug typeSMILESDrug group

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Biochemical Features of Small Molecules


check button ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)
ZINC IDmol_MWdipoleSASAFOSAFISAPISAWPSAvolumedonorHBaccptHBIPHuman Oral AbsorptionPercent Human Oral AbsorptionRule Of FiveRule Of Three


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Drug Toxicity Information


check button Toxicity information of individual drugs using eToxPred
ZINC IDSmileSurface AccessibilityToxicity


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
BRAFYWHAB, YWHAG, YWHAQ, YWHAZ, SFN, HRAS, AKT1, MAPK3, RAP1GAP, RAF1, MRAS, RAP1A, PAK2, TERF1, CCDC88A, NEDD4L, Nedd4, MAP2K1, RNF149, KSR1, BRAP, PRKCE, RPS6KB2, HSP90AA1, BRAF, YWHAE, HSPA5, MAP2K2, HSPA1A, HSPA8, YWHAH, HSPA9, ARAF, CDC37, HSP90AB1, PHKB, LIMK1, IQGAP1, MAPK1, BAD, LIPF, MUS81, Vps4b, FBXW7, FGFR2, BRCA2, VHL, FNTA, HDAC2, PIK3CA, EGFR, PTEN, FNIP1, FNIP2, RAB3GAP1, KRAS, KIAA0141, FKBPL, ARMCX3, KCNC4, RPTOR, CYLD, NRAS, HSPA4, DNAJB6, PDCD11, PIP5K1A, DNAJC15, VANGL1, DNAJC11, FKBP5, HSPA4L, HSP90B1, GNAI2, DNAJC13, GNAS, PPP2CB, HSD11B2, DNAJB11, PLD2, RAP1B, WDR6, CPNE3, MYOF, COPA, UBLCP1, PPP1CA, RAD50, PIP4K2C, PHB, VIM, PGAM1, DNAJA1, MAP2K7, SPRY2, ALDOA, AP2B1, ATP5A1, SSB, IGF1R, JUP, PPP6C, PARP1, HSPB1, NME2, PRDX2, CCT7, RAB1A, FARSA, FASN, EPRS, TRAF2, REST, KIAA1429, NANOG, ITCH, SMURF2, WWP1, WWP2, PPP2CA, PPP2R2A, AURKA, LATS2, MAP2K3, MAP2K6, RASSF1, STK11, TERT, PEBP1, CRBN, FAR1, PSMC4, UBA52, UBB, UBC, RPS27A, HSPA6, HSP90AB3P, DSP, ATAD3A, ATAD3B, P4HB, SDF2L1, SLC25A22, SPTBN4, TMEM33, CTSB, NCL, HPX, TXNDC12, SLC25A11, NDUFA4, CTSV, FBP1, HSD17B3, ZNF189, ZNF510, KIF14, SRC, TRAP1, JTB, S100P, USP28,
MKRN1TERT, CSNK1G1, DUSP23, MAP1LC3B, UBE2D3, UBE2W, FADD, TP53, CDKN1A, EXOSC8, TRA2A, LUC7L, LUC7L2, SNIP1, IGF2BP1, ELAVL1, Igf2bp1, Hnrnpf, Hnrnpa3, Caprin1, Hnrnpa1, Hnrnpu, Ybx1, Syncrip, L1td1, Pabpc1, Rbmx, Elavl1, Hnrnpa2b1, Larp1, G3bp2, Hnrnpab, Atad3a, Nufip2, Igf2bp3, Mkrn1, Dhx9, Hnrnpd, G3bp1, Lin28a, Pabpn1, Atxn2l, Matr3, Upf1, Hnrnpl, Eif4g1, Usp10, Ddx1, Larp4, Larp4b, 2700060E02Rik, Fip1l1, Ilf2, Prrc2c, Hadha, Cpsf6, Fam98a, Ago2, Ddx3x, Mov10, Casc3, Fxr1, Srrt, PABPC1, SNRNP70, GLTSCR2, RRS1, CELF3, RBFOX1, ESR2, APC, AXIN1, SLC13A1, UBE2D1, UBE2D4, OTUB2, NAA10, BYSL, RAD23B, SDCBP, UBASH3A, FAM161A, RAD23A, MTURN, YOD1, DAXX, E7, ESR1, ASH2L, MKRN2, RPS9, RPL10, RPL17, HNRNPM, STAU2, HNRNPU, SPG7, ATXN2, RPL23A, IGSF9B, RPL31, SYNCRIP, HNRNPD, RPL26, IGF2BP2, TNRC6B, RPS2, HELZ, RPS11, IGF2BP3, AKAP8, U2AF1, GSPT1, YBX3, EIF2AK2, U2AF2, RPL3, RPL35, PSMD4, VCP, RPS8, RPS13, RPS4X, RPS26, YBX1, PURA, ILF3, TRIM25, C17orf85, NUFIP2, ATXN2L, LARP4B, STRBP, AGO2, LARP1, MOV10, PABPC4, PUM1, TIAL1, UPF1, MKRN1, RPS10, AIMP1, BCAS2, BRIX1, CDC5L, CKAP5, DARS, DDX24, DHX30, DRG1, EPRS, HUWE1, IARS, KARS, KHDRBS1, KRI1, LARS, MDC1, MKI67, MRPL13, NAT10, PES1, PHIP, PRPF19, PRPF8, KIAA0020, QARS, RARS, RPF2, RPL5, RRP1B, SF3B1, SFPQ, SMARCA5, SMC1A, SMC3, SMCHD1, SON, SUPT16H, THRAP3, TOP2A, MKRN3, FZR1, TSC2, PAIP2B, SRSF3, HNRNPUL2, PRR3, PURG, FAM120A, PABPC5, HNRNPCL1, NQO1, C11orf31, ZNF346, GAPDHS, YBX2, NUPL2, LIN28B, RSL1D1, RBMS2, HNRNPC, PAIP1, APOBEC3D, GSPT2, KCNH1,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
MKRN1all structure
BRAFall structure


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to MKRN1-BRAF


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to MKRN1-BRAF


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID
MKRN1BRAFThyroid Gland Papillary CarcinomaMyCancerGenome
MKRN1BRAFColon AdenocarcinomaMyCancerGenome
MKRN1BRAFLow-Grade GliomaMyCancerGenome
MKRN1BRAFNosMyCancerGenome
MKRN1BRAFPancreatic AdenocarcinomaMyCancerGenome
MKRN1BRAFPilocytic AstrocytomaMyCancerGenome

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneBRAFC0025202melanoma24CGI;CTD_human;UNIPROT
TgeneBRAFC1275081Cardio-facio-cutaneous syndrome14CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneBRAFC0009402Colorectal Carcinoma8CTD_human;UNIPROT
TgeneBRAFC0028326Noonan Syndrome8CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET
TgeneBRAFC0238463Papillary thyroid carcinoma8CTD_human;ORPHANET
TgeneBRAFC0040136Thyroid Neoplasm6CGI;CTD_human
TgeneBRAFC0151468Thyroid Gland Follicular Adenoma6CTD_human
TgeneBRAFC0175704LEOPARD Syndrome6CLINGEN;GENOMICS_ENGLAND
TgeneBRAFC0549473Thyroid carcinoma6CGI;CTD_human
TgeneBRAFC3150970NOONAN SYNDROME 75CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneBRAFC0009404Colorectal Neoplasms4CTD_human
TgeneBRAFC3150971LEOPARD SYNDROME 34CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneBRAFC1519086Pilomyxoid astrocytoma3ORPHANET
TgeneBRAFC0004565Melanoma, B162CTD_human
TgeneBRAFC0009075Melanoma, Cloudman S912CTD_human
TgeneBRAFC0018598Melanoma, Harding-Passey2CTD_human
TgeneBRAFC0023443Hairy Cell Leukemia2CGI;ORPHANET
TgeneBRAFC0025205Melanoma, Experimental2CTD_human
TgeneBRAFC0033578Prostatic Neoplasms2CTD_human
TgeneBRAFC0152013Adenocarcinoma of lung (disorder)2CGI;CTD_human
TgeneBRAFC0376358Malignant neoplasm of prostate2CTD_human
TgeneBRAFC0587248Costello syndrome (disorder)2CLINGEN;CTD_human
TgeneBRAFC3501843Nonmedullary Thyroid Carcinoma2CTD_human
TgeneBRAFC3501844Familial Nonmedullary Thyroid Cancer2CTD_human
TgeneBRAFC0002448Ameloblastoma1CTD_human
TgeneBRAFC0004114Astrocytoma1CTD_human
TgeneBRAFC0010276Craniopharyngioma1CTD_human;ORPHANET
TgeneBRAFC0011860Diabetes Mellitus, Non-Insulin-Dependent1CTD_human
TgeneBRAFC0017638Glioma1CGI;CTD_human
TgeneBRAFC0019621Histiocytosis, Langerhans-Cell1CGI;ORPHANET
TgeneBRAFC0022665Kidney Neoplasm1CTD_human
TgeneBRAFC0023903Liver neoplasms1CTD_human
TgeneBRAFC0024232Lymphatic Metastasis1CTD_human
TgeneBRAFC0024694Mandibular Neoplasms1CTD_human
TgeneBRAFC0027659Neoplasms, Experimental1CTD_human
TgeneBRAFC0027962Melanocytic nevus1GENOMICS_ENGLAND
TgeneBRAFC0036920Sezary Syndrome1CTD_human
TgeneBRAFC0041409Turner Syndrome, Male1CTD_human
TgeneBRAFC0079773Lymphoma, T-Cell, Cutaneous1CTD_human
TgeneBRAFC0205768Subependymal Giant Cell Astrocytoma1CTD_human
TgeneBRAFC0206686Adrenocortical carcinoma1CTD_human
TgeneBRAFC0206754Neuroendocrine Tumors1CTD_human
TgeneBRAFC0259783mixed gliomas1CTD_human
TgeneBRAFC0278875Adult Craniopharyngioma1CTD_human
TgeneBRAFC0280783Juvenile Pilocytic Astrocytoma1CTD_human
TgeneBRAFC0280785Diffuse Astrocytoma1CTD_human
TgeneBRAFC0334579Anaplastic astrocytoma1CGI;CTD_human
TgeneBRAFC0334580Protoplasmic astrocytoma1CTD_human
TgeneBRAFC0334581Gemistocytic astrocytoma1CTD_human
TgeneBRAFC0334582Fibrillary Astrocytoma1CTD_human
TgeneBRAFC0334583Pilocytic Astrocytoma1CGI;CTD_human
TgeneBRAFC0338070Childhood Cerebral Astrocytoma1CTD_human
TgeneBRAFC0345904Malignant neoplasm of liver1CTD_human
TgeneBRAFC0376407Granulomatous Slack Skin1CTD_human
TgeneBRAFC0406803Syringocystadenoma Papilliferum1GENOMICS_ENGLAND
TgeneBRAFC0431128Papillary craniopharyngioma1CTD_human
TgeneBRAFC0431129Adamantinous Craniopharyngioma1CTD_human
TgeneBRAFC0547065Mixed oligoastrocytoma1CTD_human
TgeneBRAFC0555198Malignant Glioma1CTD_human
TgeneBRAFC0596263Carcinogenesis1CTD_human
TgeneBRAFC0684249Carcinoma of lung1CGI;UNIPROT
TgeneBRAFC0740457Malignant neoplasm of kidney1CTD_human
TgeneBRAFC0750935Cerebral Astrocytoma1CTD_human
TgeneBRAFC0750936Intracranial Astrocytoma1CTD_human
TgeneBRAFC0751061Craniopharyngioma, Child1CTD_human
TgeneBRAFC0920269Microsatellite Instability1CTD_human
TgeneBRAFC1527404Female Pseudo-Turner Syndrome1CTD_human
TgeneBRAFC1704230Grade I Astrocytoma1CTD_human
TgeneBRAFC1721098Replication Error Phenotype1CTD_human
TgeneBRAFC2239176Liver carcinoma1CTD_human
TgeneBRAFC4551484Leopard Syndrome 11GENOMICS_ENGLAND
TgeneBRAFC4551602Noonan Syndrome 11CTD_human
TgeneBRAFC4721532Lymphoma, Non-Hodgkin, Familial1UNIPROT
TgeneBRAFC4733333familial non-medullary thyroid cancer1GENOMICS_ENGLAND