UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:API5-FOLH1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: API5-FOLH1
	FusionPDB ID: 5420
	FusionGDB2.0 ID: 5420
		Hgene	Tgene
	Gene symbol	API5	FOLH1
	Gene ID	8539	2346
	Gene name	apoptosis inhibitor 5	folate hydrolase 1
	Synonyms	AAC-11\|AAC11	FGCP\|FOLH\|GCP2\|GCPII\|NAALAD1\|NAALAdase\|PSM\|PSMA\|mGCP
	Cytomap	11p12	11p11.12
	Type of gene	protein-coding	protein-coding
	Description	apoptosis inhibitor 5FIFantiapoptosis clone 11 proteincell migration-inducing gene 8 proteinfibroblast growth factor 2-interacting factor 2migration-inducing protein MIG8	glutamate carboxypeptidase 2N-acetylated alpha-linked acidic dipeptidase 1N-acetylated-alpha-linked acidic dipeptidase INAALADase Icell growth-inhibiting gene 27 proteinfolylpoly-gamma-glutamate carboxypeptidaseglutamate carboxylase IIglutamate car
	Modification date	20200313	20200313
	UniProtAcc	Q9BZZ5	Q9HBA9
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000529334, ENST00000378852, ENST00000420461, ENST00000455725, ENST00000531273, ENST00000534600, ENST00000534695,	ENST00000256999, ENST00000340334, ENST00000343844, ENST00000356696, ENST00000533034, ENST00000525629,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	7 X 5 X 5=175	7 X 6 X 5=210
	# samples	7	7
	** MAII score	log2(7/175*10)=-1.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/210*10)=-1.58496250072116 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: API5 [Title/Abstract] AND FOLH1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	API5(43357544)-FOLH1(49168497), # samples:2
Anticipated loss of major functional domain due to fusion event.	API5-FOLH1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. API5-FOLH1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. API5-FOLH1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. API5-FOLH1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. API5-FOLH1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. API5-FOLH1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	FOLH1	GO:0006508	proteolysis	12949938
Tgene	FOLH1	GO:0035609	C-terminal protein deglutamylation	12949938\|17241121\|24863754

Fusion gene breakpoints across API5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across FOLH1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUSC	TCGA-39-5016-01A	API5	chr11	43357544	+	FOLH1	chr11	49168497	-
ChimerDB4	LUSC	TCGA-39-5016	API5	chr11	43357544	+	FOLH1	chr11	49168497	-

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000455725	API5	chr11	43357544	+	ENST00000343844	FOLH1	chr11	49168497	-	2014	1704	245	1825	526
ENST00000531273	API5	chr11	43357544	+	ENST00000343844	FOLH1	chr11	49168497	-	1941	1631	121	1752	543
ENST00000420461	API5	chr11	43357544	+	ENST00000343844	FOLH1	chr11	49168497	-	1770	1460	112	1581	489
ENST00000378852	API5	chr11	43357544	+	ENST00000343844	FOLH1	chr11	49168497	-	1927	1617	107	1738	543
ENST00000534600	API5	chr11	43357544	+	ENST00000340334	FOLH1	chr11	49168497	-	1933	1623	14	1390	458
ENST00000534600	API5	chr11	43357544	+	ENST00000533034	FOLH1	chr11	49168497	-	1818	1623	14	1390	458

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000455725	ENST00000343844	API5	chr11	43357544	+	FOLH1	chr11	49168497	-	0.002748607	0.99725133
ENST00000531273	ENST00000343844	API5	chr11	43357544	+	FOLH1	chr11	49168497	-	0.001128636	0.9988714
ENST00000420461	ENST00000343844	API5	chr11	43357544	+	FOLH1	chr11	49168497	-	0.001245286	0.99875474
ENST00000378852	ENST00000343844	API5	chr11	43357544	+	FOLH1	chr11	49168497	-	0.001146797	0.9988532
ENST00000534600	ENST00000340334	API5	chr11	43357544	+	FOLH1	chr11	49168497	-	0.000448475	0.9995515
ENST00000534600	ENST00000533034	API5	chr11	43357544	+	FOLH1	chr11	49168497	-	0.00059217	0.9994079

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>5420_5420_1_API5-FOLH1_API5_chr11_43357544_ENST00000378852_FOLH1_chr11_49168497_ENST00000343844_length(amino acids)=543AA_BP=503
MGLSLTMPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIK
ELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEEL
ILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE
QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLG
RKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWK
PVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYGMSSMLQAATTSMQGSHSQEFMMLCLILKAKWTLPRP

--------------------------------------------------------------

>5420_5420_2_API5-FOLH1_API5_chr11_43357544_ENST00000420461_FOLH1_chr11_49168497_ENST00000343844_length(amino acids)=489AA_BP=449
MGLSLTMPTVEELYRNYGILADATEQVGQIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFS
QILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFN
PSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCEQVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYM
PLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEEN
KIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYG

--------------------------------------------------------------

>5420_5420_3_API5-FOLH1_API5_chr11_43357544_ENST00000455725_FOLH1_chr11_49168497_ENST00000343844_length(amino acids)=526AA_BP=486
MKQRWRENGIGKHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIKELPQFATGENLPRVADI
LTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEF
VLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCEQVLPNLGTLTTPVEGLD
IQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLK
DFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTT

--------------------------------------------------------------

>5420_5420_4_API5-FOLH1_API5_chr11_43357544_ENST00000531273_FOLH1_chr11_49168497_ENST00000343844_length(amino acids)=543AA_BP=503
MGLSLTMPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIK
ELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEEL
ILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE
QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLG
RKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWK
PVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYGMSSMLQAATTSMQGSHSQEFMMLCLILKAKWTLPRP

--------------------------------------------------------------

>5420_5420_5_API5-FOLH1_API5_chr11_43357544_ENST00000534600_FOLH1_chr11_49168497_ENST00000340334_length(amino acids)=458AA_BP=
MGLSLTMPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIK
ELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEEL
ILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE
QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLG
RKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWK

--------------------------------------------------------------

>5420_5420_6_API5-FOLH1_API5_chr11_43357544_ENST00000534600_FOLH1_chr11_49168497_ENST00000533034_length(amino acids)=458AA_BP=
MGLSLTMPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIK
ELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEEL
ILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE
QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLG
RKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWK

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:43357544/chr11:49168497)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
API5 Q9BZZ5	FOLH1 Q9HBA9
FUNCTION: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. {ECO:0000269\|PubMed:10780674, ECO:0000269\|PubMed:17112319, ECO:0000269\|PubMed:19387494}.	FUNCTION: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. {ECO:0000250}.; FUNCTION: Exhibits a dipeptidyl-peptidase IV type activity. {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000378852

454_475

497.3333333333333

505.0

Motif

Nuclear localization signal

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000531273

454_475

497.3333333333333

525.0

Motif

Nuclear localization signal

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000378852

2_360

497.3333333333333

505.0

Region

Note=ARM-like and Heat-like helical repeats

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000378852

370_391

497.3333333333333

505.0

Region

Note=Leucine-zipper

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000420461

2_360

443.3333333333333

451.0

Region

Note=ARM-like and Heat-like helical repeats

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000420461

370_391

443.3333333333333

451.0

Region

Note=Leucine-zipper

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000531273

2_360

497.3333333333333

525.0

Region

Note=ARM-like and Heat-like helical repeats

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000531273

370_391

497.3333333333333

525.0

Region

Note=Leucine-zipper

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

699_700

687.6666666666666

751.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

699_700

672.6666666666666

736.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

517_518

379.6666666666667

443.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

534_536

379.6666666666667

443.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

552_553

379.6666666666667

443.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

699_700

379.6666666666667

443.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

699_700

656.6666666666666

720.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

699_700

641.6666666666666

705.0

Region

Substrate binding

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

API5

chr11:43357544

chr11:49168497

ENST00000420461

454_475

443.3333333333333

451.0

Motif

Nuclear localization signal

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

146_149

687.6666666666666

751.0

Compositional bias

Poly-Pro

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

146_149

672.6666666666666

736.0

Compositional bias

Poly-Pro

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

146_149

379.6666666666667

443.0

Compositional bias

Poly-Pro

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

146_149

656.6666666666666

720.0

Compositional bias

Poly-Pro

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

146_149

641.6666666666666

705.0

Compositional bias

Poly-Pro

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

274_587

687.6666666666666

751.0

Region

Note=NAALADase

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

517_518

687.6666666666666

751.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

534_536

687.6666666666666

751.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

552_553

687.6666666666666

751.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

274_587

672.6666666666666

736.0

Region

Note=NAALADase

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

517_518

672.6666666666666

736.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

534_536

672.6666666666666

736.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

552_553

672.6666666666666

736.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

274_587

379.6666666666667

443.0

Region

Note=NAALADase

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

274_587

656.6666666666666

720.0

Region

Note=NAALADase

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

517_518

656.6666666666666

720.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

534_536

656.6666666666666

720.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

552_553

656.6666666666666

720.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

274_587

641.6666666666666

705.0

Region

Note=NAALADase

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

517_518

641.6666666666666

705.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

534_536

641.6666666666666

705.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

552_553

641.6666666666666

705.0

Region

Substrate binding

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

1_19

687.6666666666666

751.0

Topological domain

Cytoplasmic

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

44_750

687.6666666666666

751.0

Topological domain

Extracellular

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

1_19

672.6666666666666

736.0

Topological domain

Cytoplasmic

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

44_750

672.6666666666666

736.0

Topological domain

Extracellular

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

1_19

379.6666666666667

443.0

Topological domain

Cytoplasmic

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

44_750

379.6666666666667

443.0

Topological domain

Extracellular

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

1_19

656.6666666666666

720.0

Topological domain

Cytoplasmic

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

44_750

656.6666666666666

720.0

Topological domain

Extracellular

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

1_19

641.6666666666666

705.0

Topological domain

Cytoplasmic

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

44_750

641.6666666666666

705.0

Topological domain

Extracellular

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000256999

20_43

687.6666666666666

751.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000340334

20_43

672.6666666666666

736.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000343844

20_43

379.6666666666667

443.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000356696

20_43

656.6666666666666

720.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

FOLH1

chr11:43357544

chr11:49168497

ENST00000533034

20_43

641.6666666666666

705.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1161_API5_43357544_FOLH1_49168497_1161_API5_43357544_FOLH1_49168497_ranked_0.pdb	API5	43357544	43357544	ENST00000533034	FOLH1	chr11	49168497	-	MGLSLTMPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIK ELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEEL ILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLG RKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWK PVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYGMSSMLQAATTSMQGSHSQEFMMLCLILKAKWTLPRP	543

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

API5_pLDDT.png

FOLH1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
API5
FOLH1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to API5-FOLH1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to API5-FOLH1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source