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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MLLT4-EXOC6B

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MLLT4-EXOC6B
	FusionPDB ID: 54305
	FusionGDB2.0 ID: 54305
		Hgene	Tgene
	Gene symbol	MLLT4	EXOC6B
	Gene ID	4301	23233
	Gene name	afadin, adherens junction formation factor	exocyst complex component 6B
	Synonyms	AF6\|MLL-AF6\|MLLT4\|l-afadin	SEC15B\|SEC15L2\|SEMDJL3
	Cytomap	6q27	2p13.2
	Type of gene	protein-coding	protein-coding
	Description	afadinALL1-fused gene from chromosome 6 proteinmyeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4protein AF-6	exocyst complex component 6BSEC15 homolog BSEC15-like protein 2exocyst complex component Sec15B
	Modification date	20200322	20200313
	UniProtAcc	.	Q9Y2D4
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000344191, ENST00000351017, ENST00000366806, ENST00000392108, ENST00000392112, ENST00000400822, ENST00000447894, ENST00000507679,	ENST00000410104, ENST00000490919, ENST00000272427,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	15 X 7 X 10=1050	10 X 8 X 6=480
	# samples	16	11
	** MAII score	log2(16/1050*10)=-2.71424551766612 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(11/480*10)=-2.12553088208386 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MLLT4 [Title/Abstract] AND EXOC6B [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MLLT4(168303066)-EXOC6B(72802797), # samples:2
Anticipated loss of major functional domain due to fusion event.	MLLT4-EXOC6B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MLLT4-EXOC6B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MLLT4-EXOC6B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MLLT4-EXOC6B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across MLLT4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across EXOC6B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-EJ-A7NF-01A	MLLT4	chr6	168303066	-	EXOC6B	chr2	72802797	-
ChimerDB4	PRAD	TCGA-EJ-A7NF	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000344191	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6940	1822	172	3588	1138
ENST00000351017	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6940	1822	172	3588	1138
ENST00000392108	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6910	1792	142	3558	1138
ENST00000392112	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6862	1744	142	3510	1122
ENST00000366806	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6910	1792	142	3558	1138
ENST00000447894	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6768	1650	0	3416	1138
ENST00000400822	MLLT4	chr6	168303066	+	ENST00000272427	EXOC6B	chr2	72802797	-	6765	1647	0	3413	1137

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000344191	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.000482824	0.9995172
ENST00000351017	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.000482824	0.9995172
ENST00000392108	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.000459358	0.9995407
ENST00000392112	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.000657964	0.999342
ENST00000366806	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.000459358	0.9995407
ENST00000447894	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.000375545	0.9996245
ENST00000400822	ENST00000272427	MLLT4	chr6	168303066	+	EXOC6B	chr2	72802797	-	0.00037506	0.99962497

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>54305_54305_1_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000344191_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1138AA_BP=548
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA
SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLST
TDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE
GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI
HSGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYS
VLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCS
DPNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQP
FPKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYL
EEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKV
AQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPN

--------------------------------------------------------------

>54305_54305_2_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000351017_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1138AA_BP=548
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA
SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLST
TDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE
GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI
HSGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYS
VLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCS
DPNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQP
FPKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYL
EEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKV
AQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPN

--------------------------------------------------------------

>54305_54305_3_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000366806_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1138AA_BP=548
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA
SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLST
TDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE
GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI
HSGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYS
VLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCS
DPNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQP
FPKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYL
EEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKV
AQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPN

--------------------------------------------------------------

>54305_54305_4_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000392108_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1138AA_BP=548
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA
SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLST
TDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE
GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI
HSGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYS
VLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCS
DPNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQP
FPKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYL
EEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKV
AQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPN

--------------------------------------------------------------

>54305_54305_5_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000392112_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1122AA_BP=532
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQAS
DKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTT
DPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEG
KTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSM
DAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKAQQQRN
LDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYSVLGARETFENYYRKQR
RKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCSDPNLVLDLKNLIVLFA
DTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQPFPKKFPFSEFVPKVYN
QIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYLEEFITNITNVLPETVH
TTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKVAQTACMSACKHLATSL
MQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPNCKYLRVNPVTALTLLE

--------------------------------------------------------------

>54305_54305_6_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000400822_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1137AA_BP=547
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQAS
DKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTT
DPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEG
KTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCD
LTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIH
SGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYSV
LGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCSD
PNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQPF
PKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYLE
EFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKVA
QTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPNC

--------------------------------------------------------------

>54305_54305_7_MLLT4-EXOC6B_MLLT4_chr6_168303066_ENST00000447894_EXOC6B_chr2_72802797_ENST00000272427_length(amino acids)=1138AA_BP=548
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK
YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA
SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLST
TDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE
GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI
HSGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYS
VLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCS
DPNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQP
FPKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYL
EEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKV
AQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPN

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:168303066/chr2:72802797)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	EXOC6B Q9Y2D4
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

146_185

550.0

1652.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

146_185

549.0

1835.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

146_185

550.0

1825.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

163_175

550.0

1652.0

Compositional bias

Note=Glu/Lys-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

163_175

549.0

1835.0

Compositional bias

Note=Glu/Lys-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

163_175

550.0

1825.0

Compositional bias

Note=Glu/Lys-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

246_348

550.0

1652.0

Domain

Ras-associating 2

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

39_133

550.0

1652.0

Domain

Ras-associating 1

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

426_492

550.0

1652.0

Domain

Note=FHA

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

246_348

549.0

1835.0

Domain

Ras-associating 2

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

39_133

549.0

1835.0

Domain

Ras-associating 1

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

426_492

549.0

1835.0

Domain

Note=FHA

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

246_348

550.0

1825.0

Domain

Ras-associating 2

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

39_133

550.0

1825.0

Domain

Ras-associating 1

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

426_492

550.0

1825.0

Domain

Note=FHA

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

1408_1448

550.0

1652.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

1523_1667

550.0

1652.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

1408_1448

549.0

1835.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

1523_1667

549.0

1835.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

1408_1448

550.0

1825.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

1523_1667

550.0

1825.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

1346_1392

550.0

1652.0

Compositional bias

Note=Pro-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

1578_1588

550.0

1652.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

1678_1708

550.0

1652.0

Compositional bias

Note=Pro-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

1346_1392

549.0

1835.0

Compositional bias

Note=Pro-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

1578_1588

549.0

1835.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

1678_1708

549.0

1835.0

Compositional bias

Note=Pro-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

1346_1392

550.0

1825.0

Compositional bias

Note=Pro-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

1578_1588

550.0

1825.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

1678_1708

550.0

1825.0

Compositional bias

Note=Pro-rich

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

1007_1093

550.0

1652.0

Domain

PDZ

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000392108

668_908

550.0

1652.0

Domain

Dilute

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

1007_1093

549.0

1835.0

Domain

PDZ

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000400822

668_908

549.0

1835.0

Domain

Dilute

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

1007_1093

550.0

1825.0

Domain

PDZ

Hgene

MLLT4

chr6:168303066

chr2:72802797

ENST00000447894

668_908

550.0

1825.0

Domain

Dilute

Tgene

EXOC6B

chr6:168303066

chr2:72802797

ENST00000272427

50_119

223.0

812.0

Coiled coil

Ontology_term=ECO:0000255

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1883_MLLT4_168303066_EXOC6B_72802797_ranked_0.pdb	MLLT4	168303066	168303066	ENST00000272427	EXOC6B	chr2	72802797	-	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPK YSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLST TDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI HSGTALPTSKAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDDEEVPGAQDLVDFSPVYRCLHIYS VLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFFVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCS DPNLVLDLKNLIVLFADTLQVYGFPVNQLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQP FPKKFPFSEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIIINTTHLEKSCKYL EEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMTGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKV AQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNLDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPN	1138

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EXOC6B_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MLLT4
EXOC6B

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to MLLT4-EXOC6B

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to MLLT4-EXOC6B

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source