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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MPDU1-GUCD1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MPDU1-GUCD1
	FusionPDB ID: 54664
	FusionGDB2.0 ID: 54664
		Hgene	Tgene
	Gene symbol	MPDU1	GUCD1
	Gene ID	9526	83606
	Gene name	mannose-P-dolichol utilization defect 1	guanylyl cyclase domain containing 1
	Synonyms	CDGIF\|HBEBP2BPA\|Lec35\|My008\|PP3958\|PQLC5\|SL15\|SLC66A5	C22orf13\|LLN4
	Cytomap	17p13.1	22q11.23
	Type of gene	protein-coding	protein-coding
	Description	mannose-P-dolichol utilization defect 1 proteinHBeAg-binding protein 2 binding protein Asuppressor of Lec15 and Lec35 glycosylation mutation homolog	protein GUCD1CG13760 gene product [Drosophila melanogaster] homologguanylyl cyclase domain-containing protein 1
	Modification date	20200320	20200313
	UniProtAcc	O75352	Q96NT3
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000250124, ENST00000396501, ENST00000423172, ENST00000582151,	ENST00000402766, ENST00000404664, ENST00000447813, ENST00000490922, ENST00000407471, ENST00000435822,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	7 X 4 X 4=112	2 X 3 X 2=12
	# samples	8	2
	** MAII score	log2(8/112*10)=-0.485426827170242 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(2/12*10)=0.736965594166206 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MPDU1 [Title/Abstract] AND GUCD1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MPDU1(7487283)-GUCD1(24944969), # samples:2
Anticipated loss of major functional domain due to fusion event.	MPDU1-GUCD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MPDU1-GUCD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MPDU1-GUCD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. MPDU1-GUCD1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MPDU1	GO:0009312	oligosaccharide biosynthetic process	11179430

Fusion gene breakpoints across MPDU1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across GUCD1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BLCA	TCGA-GV-A3JW-01A	MPDU1	chr17	7487283	+	GUCD1	chr22	24944041	-
ChimerDB4	BLCA	TCGA-GV-A3JW-01A	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000582151	MPDU1	chr17	7487283	+	ENST00000407471	GUCD1	chr22	24944969	-	3539	291	1525	278	415
ENST00000582151	MPDU1	chr17	7487283	+	ENST00000435822	GUCD1	chr22	24944969	-	3536	291	1522	278	414
ENST00000250124	MPDU1	chr17	7487283	+	ENST00000407471	GUCD1	chr22	24944969	-	3567	319	1553	267	428
ENST00000250124	MPDU1	chr17	7487283	+	ENST00000435822	GUCD1	chr22	24944969	-	3564	319	1550	267	427
ENST00000396501	MPDU1	chr17	7487283	+	ENST00000407471	GUCD1	chr22	24944969	-	3386	138	1372	86	428
ENST00000396501	MPDU1	chr17	7487283	+	ENST00000435822	GUCD1	chr22	24944969	-	3383	138	1369	86	427
ENST00000423172	MPDU1	chr17	7487283	+	ENST00000407471	GUCD1	chr22	24944969	-	3361	113	1347	61	428
ENST00000423172	MPDU1	chr17	7487283	+	ENST00000435822	GUCD1	chr22	24944969	-	3358	113	1344	61	427

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000582151	ENST00000407471	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.007964289	0.9920357
ENST00000582151	ENST00000435822	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.011210577	0.98878944
ENST00000250124	ENST00000407471	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.021405196	0.97859484
ENST00000250124	ENST00000435822	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.018826764	0.9811732
ENST00000396501	ENST00000407471	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.02296421	0.9770358
ENST00000396501	ENST00000435822	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.020281222	0.9797188
ENST00000423172	ENST00000407471	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.022506075	0.977494
ENST00000423172	ENST00000435822	MPDU1	chr17	7487283	+	GUCD1	chr22	24944969	-	0.019856574	0.98014337

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>54664_54664_1_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000250124_GUCD1_chr22_24944969_ENST00000407471_length(amino acids)=428AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSWVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVT
VQHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGP

--------------------------------------------------------------

>54664_54664_2_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000250124_GUCD1_chr22_24944969_ENST00000435822_length(amino acids)=427AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVTV
QHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGPD

--------------------------------------------------------------

>54664_54664_3_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000396501_GUCD1_chr22_24944969_ENST00000407471_length(amino acids)=428AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSWVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVT
VQHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGP

--------------------------------------------------------------

>54664_54664_4_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000396501_GUCD1_chr22_24944969_ENST00000435822_length(amino acids)=427AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVTV
QHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGPD

--------------------------------------------------------------

>54664_54664_5_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000423172_GUCD1_chr22_24944969_ENST00000407471_length(amino acids)=428AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSWVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVT
VQHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGP

--------------------------------------------------------------

>54664_54664_6_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000423172_GUCD1_chr22_24944969_ENST00000435822_length(amino acids)=427AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVTV
QHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGPD

--------------------------------------------------------------

>54664_54664_7_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000582151_GUCD1_chr22_24944969_ENST00000407471_length(amino acids)=415AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSWVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVT
VQHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGP

--------------------------------------------------------------

>54664_54664_8_MPDU1-GUCD1_MPDU1_chr17_7487283_ENST00000582151_GUCD1_chr22_24944969_ENST00000435822_length(amino acids)=414AA_BP=0
MSHRNLGPWVGRWWWYHQPLGTGAYAALPGERGLEGSSALGDWGQLCCLQTLTYCPDKWHPSSNFSLQAQSCGLWGDHCHGSGSKATRAL
SGLDFLWAQLESRLGVLGYASSSQQVLGVSDMTTHGTGRQSSCASLRVPGTLPDGLRLQFHIPTPAAPSLGQGILSGPGWGGDGVRGPRR
TRLLLSAVQVDKEDVLICAIAGSGLLKVTDAGAAHSVGIGWVVVEDAASGPVVATQHDEVALVVRSAAEAVVATGGEAAVLDRAGAQVTV
QHPRVHQHDGHMALSQVRLDVLHTHCAFLHQHLAGLCLCKQLIHPGLFCVKVLPVEGLVLVALVDTQGLGTEAVPHAKVVHQVGQVDGPD

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:7487283/chr22:24944969)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MPDU1 O75352	GUCD1 Q96NT3
FUNCTION: Required for normal utilization of mannose-dolichol phosphate (Dol-P-Man) in the synthesis of N-linked and O-linked oligosaccharides and GPI anchors. {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

159_216

34.333333333333336

248.0

Domain

Note=PQ-loop 2

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

39_105

34.333333333333336

248.0

Domain

Note=PQ-loop 1

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

100_120

34.333333333333336

248.0

Transmembrane

Helical

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

128_145

34.333333333333336

248.0

Transmembrane

Helical

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

151_171

34.333333333333336

248.0

Transmembrane

Helical

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

185_205

34.333333333333336

248.0

Transmembrane

Helical

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

213_233

34.333333333333336

248.0

Transmembrane

Helical

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

37_57

34.333333333333336

248.0

Transmembrane

Helical

Hgene

MPDU1

chr17:7487283

chr22:24944969

ENST00000250124

74_94

34.333333333333336

248.0

Transmembrane

Helical

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MPDU1
GUCD1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to MPDU1-GUCD1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to MPDU1-GUCD1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source