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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NFATC3-SPATA33

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NFATC3-SPATA33
	FusionPDB ID: 58810
	FusionGDB2.0 ID: 58810
		Hgene	Tgene
	Gene symbol	NFATC3	SPATA33
	Gene ID	4775	124045
	Gene name	nuclear factor of activated T cells 3	spermatogenesis associated 33
	Synonyms	NF-AT4c\|NFAT4\|NFATX	C16orf55
	Cytomap	16q22.1	16q24.3
	Type of gene	protein-coding	protein-coding
	Description	nuclear factor of activated T-cells, cytoplasmic 3NF-ATc3T cell transcription factor NFAT4nuclear factor of activated T-cells c3 isoform IE-Xanuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3	spermatogenesis-associated protein 33
	Modification date	20200313	20200313
	UniProtAcc	Q12968	Q96N06
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000535127, ENST00000329524, ENST00000346183, ENST00000349223, ENST00000575270,	ENST00000301031, ENST00000579310, ENST00000566857, ENST00000568929,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	9 X 4 X 9=324	3 X 1 X 3=9
	# samples	11	3
	** MAII score	log2(11/324*10)=-1.55849028935997 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(3/9*10)=1.73696559416621 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NFATC3 [Title/Abstract] AND SPATA33 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NFATC3(68225678)-SPATA33(89735694), # samples:2
Anticipated loss of major functional domain due to fusion event.	NFATC3-SPATA33 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFATC3-SPATA33 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFATC3-SPATA33 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NFATC3-SPATA33 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NFATC3-SPATA33 seems lost the major protein functional domain in Hgene partner, which is a transcription factor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	NFATC3	GO:0045944	positive regulation of transcription by RNA polymerase II	18815128
Hgene	NFATC3	GO:1905064	negative regulation of vascular smooth muscle cell differentiation	23853098

Fusion gene breakpoints across NFATC3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across SPATA33 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-M7-A724-01A	NFATC3	chr16	68225678	+	SPATA33	chr16	89735694	+
ChimerDB4	PRAD	TCGA-M7-A724	NFATC3	chr16	68225678	+	SPATA33	chr16	89735694	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000575270

NFATC3

chr16

68225678

ENST00000579310

SPATA33

chr16

89735694

4456

3444

338

3655

1105

ENST00000349223

NFATC3

chr16

68225678

ENST00000579310

SPATA33

chr16

89735694

4342

3330

224

3541

1105

ENST00000329524

NFATC3

chr16

68225678

ENST00000579310

SPATA33

chr16

89735694

4142

3130

3341

1105

ENST00000346183

NFATC3

chr16

68225678

ENST00000579310

SPATA33

chr16

89735694

4142

3130

3341

1105

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000575270	ENST00000579310	NFATC3	chr16	68225678	+	SPATA33	chr16	89735694	+	0.002443733	0.9975562
ENST00000349223	ENST00000579310	NFATC3	chr16	68225678	+	SPATA33	chr16	89735694	+	0.002253652	0.9977463
ENST00000329524	ENST00000579310	NFATC3	chr16	68225678	+	SPATA33	chr16	89735694	+	0.001715232	0.99828476
ENST00000346183	ENST00000579310	NFATC3	chr16	68225678	+	SPATA33	chr16	89735694	+	0.001715232	0.99828476

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>58810_58810_1_NFATC3-SPATA33_NFATC3_chr16_68225678_ENST00000329524_SPATA33_chr16_89735694_ENST00000579310_length(amino acids)=1105AA_BP=1035
MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTC
EIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDAS
SCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSP
CGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSD
DQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETE
GSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGI
LKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESK
IIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPV
PHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVV
YNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVAD
QITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPS
SLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDEKPDVKQKSSRKKVVVPQIIITRASNETLVSCSSSGSDQQRTIRE

--------------------------------------------------------------

>58810_58810_2_NFATC3-SPATA33_NFATC3_chr16_68225678_ENST00000346183_SPATA33_chr16_89735694_ENST00000579310_length(amino acids)=1105AA_BP=1035
MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTC
EIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDAS
SCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSP
CGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSD
DQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETE
GSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGI
LKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESK
IIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPV
PHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVV
YNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVAD
QITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPS
SLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDEKPDVKQKSSRKKVVVPQIIITRASNETLVSCSSSGSDQQRTIRE

--------------------------------------------------------------

>58810_58810_3_NFATC3-SPATA33_NFATC3_chr16_68225678_ENST00000349223_SPATA33_chr16_89735694_ENST00000579310_length(amino acids)=1105AA_BP=1035
MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTC
EIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDAS
SCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSP
CGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSD
DQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETE
GSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGI
LKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESK
IIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPV
PHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVV
YNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVAD
QITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPS
SLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDEKPDVKQKSSRKKVVVPQIIITRASNETLVSCSSSGSDQQRTIRE

--------------------------------------------------------------

>58810_58810_4_NFATC3-SPATA33_NFATC3_chr16_68225678_ENST00000575270_SPATA33_chr16_89735694_ENST00000579310_length(amino acids)=1105AA_BP=1035
MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTC
EIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDAS
SCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSP
CGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSD
DQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETE
GSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGI
LKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESK
IIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPV
PHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVV
YNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVAD
QITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPS
SLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDEKPDVKQKSSRKKVVVPQIIITRASNETLVSCSSSGSDQQRTIRE

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:68225678/chr16:89735694)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NFATC3 Q12968	SPATA33 Q96N06
FUNCTION: Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 (PubMed:18815128). Along with NFATC4, involved in embryonic heart development (By similarity). {ECO:0000250\|UniProtKB:P97305, ECO:0000269\|PubMed:18815128}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

24_29

1035.3333333333333

1996.6666666666667

Compositional bias

Note=Poly-Pro

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

24_29

1035.3333333333333

1076.0

Compositional bias

Note=Poly-Pro

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

24_29

1035.3333333333333

1066.0

Compositional bias

Note=Poly-Pro

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

444_451

1035.3333333333333

1996.6666666666667

DNA binding

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

444_451

1035.3333333333333

1076.0

DNA binding

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

444_451

1035.3333333333333

1066.0

DNA binding

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

415_596

1035.3333333333333

1996.6666666666667

Domain

RHD

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

415_596

1035.3333333333333

1076.0

Domain

RHD

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

415_596

1035.3333333333333

1066.0

Domain

RHD

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

273_275

1035.3333333333333

1996.6666666666667

Motif

Note=Nuclear localization signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

686_688

1035.3333333333333

1996.6666666666667

Motif

Note=Nuclear localization signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

273_275

1035.3333333333333

1076.0

Motif

Note=Nuclear localization signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

686_688

1035.3333333333333

1076.0

Motif

Note=Nuclear localization signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

273_275

1035.3333333333333

1066.0

Motif

Note=Nuclear localization signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

686_688

1035.3333333333333

1066.0

Motif

Note=Nuclear localization signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

109_114

1035.3333333333333

1996.6666666666667

Region

Note=Calcineurin-binding

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

207_308

1035.3333333333333

1996.6666666666667

Region

Note=3 X SP repeats

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

109_114

1035.3333333333333

1076.0

Region

Note=Calcineurin-binding

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

207_308

1035.3333333333333

1076.0

Region

Note=3 X SP repeats

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

109_114

1035.3333333333333

1066.0

Region

Note=Calcineurin-binding

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

207_308

1035.3333333333333

1066.0

Region

Note=3 X SP repeats

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

207_223

1035.3333333333333

1996.6666666666667

Repeat

Note=1

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

236_252

1035.3333333333333

1996.6666666666667

Repeat

Note=2

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

292_308

1035.3333333333333

1996.6666666666667

Repeat

Note=3

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

207_223

1035.3333333333333

1076.0

Repeat

Note=1

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

236_252

1035.3333333333333

1076.0

Repeat

Note=2

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

292_308

1035.3333333333333

1076.0

Repeat

Note=3

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

207_223

1035.3333333333333

1066.0

Repeat

Note=1

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

236_252

1035.3333333333333

1066.0

Repeat

Note=2

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

292_308

1035.3333333333333

1066.0

Repeat

Note=3

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000329524

1032_1041

1035.3333333333333

1996.6666666666667

Motif

Note=Nuclear export signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000346183

1032_1041

1035.3333333333333

1076.0

Motif

Note=Nuclear export signal

Hgene

NFATC3

chr16:68225678

chr16:89735694

ENST00000349223

1032_1041

1035.3333333333333

1066.0

Motif

Note=Nuclear export signal

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1866_NFATC3_68225678_SPATA33_89735694_ranked_0.pdb	NFATC3	68225678	68225678	ENST00000579310	SPATA33	chr16	89735694	+	MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTC EIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDAS SCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSP CGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSD DQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETE GSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGI LKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESK IIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPV PHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVV YNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVAD QITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPS SLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDEKPDVKQKSSRKKVVVPQIIITRASNETLVSCSSSGSDQQRTIRE	1105

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NFATC3_pLDDT.png

SPATA33_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NFATC3
SPATA33

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to NFATC3-SPATA33

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NFATC3-SPATA33

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source