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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Potential Active Site Information
	Potentially Interacting Small Molecules through Virtual Screening
	Biochemical Features of Small Molecules with ADME
	Drug Toxicity Information
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NPM1-RARA

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NPM1-RARA
	FusionPDB ID: 59987
	FusionGDB2.0 ID: 59987
		Hgene	Tgene
	Gene symbol	NPM1	RARA
	Gene ID	4869	5914
	Gene name	nucleophosmin 1	retinoic acid receptor alpha
	Synonyms	B23\|NPM	NR1B1\|RAR
	Cytomap	5q35.1	17q21.2
	Type of gene	protein-coding	protein-coding
	Description	nucleophosminnucleolar protein NO38nucleophosmin (nucleolar phosphoprotein B23, numatrin)nucleophosmin/nucleoplasmin family, member 1testicular tissue protein Li 128	retinoic acid receptor alphaRAR-alphanuclear receptor subfamily 1 group B member 1nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long formretinoic acid nuclear receptor alpha variant 1retinoic acid nuclear receptor alpha variant 2
	Modification date	20200329	20200327
	UniProtAcc	P06748	P10276
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000296930, ENST00000351986, ENST00000393820, ENST00000517671,	ENST00000394081, ENST00000394086, ENST00000420042, ENST00000425707, ENST00000254066, ENST00000394089,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	21 X 25 X 6=3150	29 X 39 X 17=19227
	# samples	32	55
	** MAII score	log2(32/3150*10)=-3.29920801838728 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(55/19227*10)=-5.12755824682814 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NPM1 [Title/Abstract] AND RARA [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NPM1(170818803)-RARA(38504565), # samples:2
Anticipated loss of major functional domain due to fusion event.	NPM1-RARA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NPM1-RARA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NPM1-RARA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NPM1-RARA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	NPM1	GO:0006281	DNA repair	19188445
Hgene	NPM1	GO:0006334	nucleosome assembly	11602260
Hgene	NPM1	GO:0006913	nucleocytoplasmic transport	16041368
Hgene	NPM1	GO:0008104	protein localization	18420587
Hgene	NPM1	GO:0008284	positive regulation of cell proliferation	22528486
Hgene	NPM1	GO:0032071	regulation of endodeoxyribonuclease activity	19188445
Hgene	NPM1	GO:0034644	cellular response to UV	19160485
Hgene	NPM1	GO:0043066	negative regulation of apoptotic process	12882984
Hgene	NPM1	GO:0044387	negative regulation of protein kinase activity by regulation of protein phosphorylation	12882984
Hgene	NPM1	GO:0045727	positive regulation of translation	12882984
Hgene	NPM1	GO:0045893	positive regulation of transcription, DNA-templated	22528486
Hgene	NPM1	GO:0045944	positive regulation of transcription by RNA polymerase II	19160485
Hgene	NPM1	GO:0060699	regulation of endoribonuclease activity	19188445
Hgene	NPM1	GO:0060735	regulation of eIF2 alpha phosphorylation by dsRNA	12882984
Hgene	NPM1	GO:1902751	positive regulation of cell cycle G2/M phase transition	22528486
Tgene	RARA	GO:0007165	signal transduction	2825025
Tgene	RARA	GO:0030853	negative regulation of granulocyte differentiation	19917671
Tgene	RARA	GO:0032689	negative regulation of interferon-gamma production	18416830
Tgene	RARA	GO:0032720	negative regulation of tumor necrosis factor production	18416830
Tgene	RARA	GO:0032736	positive regulation of interleukin-13 production	18416830
Tgene	RARA	GO:0032753	positive regulation of interleukin-4 production	18416830
Tgene	RARA	GO:0032754	positive regulation of interleukin-5 production	18416830
Tgene	RARA	GO:0045630	positive regulation of T-helper 2 cell differentiation	18416830
Tgene	RARA	GO:0045892	negative regulation of transcription, DNA-templated	20080953
Tgene	RARA	GO:0045893	positive regulation of transcription, DNA-templated	18845237\|19850744\|20080953
Tgene	RARA	GO:0045944	positive regulation of transcription by RNA polymerase II	19850744\|21131358
Tgene	RARA	GO:0071300	cellular response to retinoic acid	19917671
Tgene	RARA	GO:0071391	cellular response to estrogen stimulus	20080953

Fusion gene breakpoints across NPM1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across RARA (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	acute non lymphocytic leukemia	U41743	NPM1	chr5	170818803		RARA	chr17	38504565
ChimerKB3	.	.	NPM1	chr5	170818803	+	RARA	chr17	38504567	+
ChiTaRS5.0	N/A	U41743	NPM1	chr5	170818803	+	RARA	chr17	38504565	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000517671	NPM1	chr5	170818803	+	ENST00000254066	RARA	chr17	38504567	+	2268	487	42	1697	551
ENST00000517671	NPM1	chr5	170818803	+	ENST00000394089	RARA	chr17	38504567	+	1803	487	42	1697	551
ENST00000296930	NPM1	chr5	170818803	+	ENST00000254066	RARA	chr17	38504567	+	2434	653	70	1863	597
ENST00000296930	NPM1	chr5	170818803	+	ENST00000394089	RARA	chr17	38504567	+	1969	653	70	1863	597
ENST00000351986	NPM1	chr5	170818803	+	ENST00000254066	RARA	chr17	38504567	+	2253	472	42	1682	546
ENST00000351986	NPM1	chr5	170818803	+	ENST00000394089	RARA	chr17	38504567	+	1788	472	42	1682	546
ENST00000393820	NPM1	chr5	170818803	+	ENST00000254066	RARA	chr17	38504567	+	2231	450	20	1660	546
ENST00000393820	NPM1	chr5	170818803	+	ENST00000394089	RARA	chr17	38504567	+	1766	450	20	1660	546
ENST00000517671	NPM1	chr5	170818803		ENST00000254066	RARA	chr17	38504565		2268	487	42	1697	551
ENST00000517671	NPM1	chr5	170818803		ENST00000394089	RARA	chr17	38504565		1803	487	42	1697	551
ENST00000296930	NPM1	chr5	170818803		ENST00000254066	RARA	chr17	38504565		2434	653	70	1863	597
ENST00000296930	NPM1	chr5	170818803		ENST00000394089	RARA	chr17	38504565		1969	653	70	1863	597
ENST00000351986	NPM1	chr5	170818803		ENST00000254066	RARA	chr17	38504565		2253	472	42	1682	546
ENST00000351986	NPM1	chr5	170818803		ENST00000394089	RARA	chr17	38504565		1788	472	42	1682	546
ENST00000393820	NPM1	chr5	170818803		ENST00000254066	RARA	chr17	38504565		2231	450	20	1660	546
ENST00000393820	NPM1	chr5	170818803		ENST00000394089	RARA	chr17	38504565		1766	450	20	1660	546

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000517671	ENST00000254066	NPM1	chr5	170818803		RARA	chr17	38504565		0.010368201	0.9896318
ENST00000517671	ENST00000394089	NPM1	chr5	170818803		RARA	chr17	38504565		0.015312034	0.984688
ENST00000296930	ENST00000254066	NPM1	chr5	170818803		RARA	chr17	38504565		0.007049596	0.99295044
ENST00000296930	ENST00000394089	NPM1	chr5	170818803		RARA	chr17	38504565		0.009892804	0.9901072
ENST00000351986	ENST00000254066	NPM1	chr5	170818803		RARA	chr17	38504565		0.010542763	0.9894573
ENST00000351986	ENST00000394089	NPM1	chr5	170818803		RARA	chr17	38504565		0.015873574	0.98412645
ENST00000393820	ENST00000254066	NPM1	chr5	170818803		RARA	chr17	38504565		0.010890275	0.98910975
ENST00000393820	ENST00000394089	NPM1	chr5	170818803		RARA	chr17	38504565		0.016287688	0.9837123

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>59987_59987_1_NPM1-RARA_NPM1_chr5_170818803_ENST00000296930_RARA_chr17_38504565_ENST00000254066_length(amino acids)=597AA_BP=194
MKSACAGRLRYGGGRASEHARGGGTWEALARSSGSIYKRGEPASFPWCDSVLRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLR
PQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLK
CGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKV
TRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLW
DKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQ
LLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGS

--------------------------------------------------------------

>59987_59987_2_NPM1-RARA_NPM1_chr5_170818803_ENST00000296930_RARA_chr17_38504565_ENST00000394089_length(amino acids)=597AA_BP=194
MKSACAGRLRYGGGRASEHARGGGTWEALARSSGSIYKRGEPASFPWCDSVLRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLR
PQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLK
CGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKV
TRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLW
DKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQ
LLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGS

--------------------------------------------------------------

>59987_59987_3_NPM1-RARA_NPM1_chr5_170818803_ENST00000296930_RARA_chr17_38504567_ENST00000254066_length(amino acids)=597AA_BP=194
MKSACAGRLRYGGGRASEHARGGGTWEALARSSGSIYKRGEPASFPWCDSVLRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLR
PQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLK
CGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKV
TRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLW
DKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQ
LLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGS

--------------------------------------------------------------

>59987_59987_4_NPM1-RARA_NPM1_chr5_170818803_ENST00000296930_RARA_chr17_38504567_ENST00000394089_length(amino acids)=597AA_BP=194
MKSACAGRLRYGGGRASEHARGGGTWEALARSSGSIYKRGEPASFPWCDSVLRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLR
PQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLK
CGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKV
TRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLW
DKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQ
LLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGS

--------------------------------------------------------------

>59987_59987_5_NPM1-RARA_NPM1_chr5_170818803_ENST00000351986_RARA_chr17_38504565_ENST00000254066_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_6_NPM1-RARA_NPM1_chr5_170818803_ENST00000351986_RARA_chr17_38504565_ENST00000394089_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_7_NPM1-RARA_NPM1_chr5_170818803_ENST00000351986_RARA_chr17_38504567_ENST00000254066_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_8_NPM1-RARA_NPM1_chr5_170818803_ENST00000351986_RARA_chr17_38504567_ENST00000394089_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_9_NPM1-RARA_NPM1_chr5_170818803_ENST00000393820_RARA_chr17_38504565_ENST00000254066_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_10_NPM1-RARA_NPM1_chr5_170818803_ENST00000393820_RARA_chr17_38504565_ENST00000394089_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_11_NPM1-RARA_NPM1_chr5_170818803_ENST00000393820_RARA_chr17_38504567_ENST00000254066_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_12_NPM1-RARA_NPM1_chr5_170818803_ENST00000393820_RARA_chr17_38504567_ENST00000394089_length(amino acids)=546AA_BP=143
MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEA
MNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSS
GYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLTPEVGE
LIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTR
YTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR
RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSS

--------------------------------------------------------------

>59987_59987_13_NPM1-RARA_NPM1_chr5_170818803_ENST00000517671_RARA_chr17_38504565_ENST00000254066_length(amino acids)=551AA_BP=148
MAPKLRKDKDFGDVFSGRTELKNKVPACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHI
VEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVC
QDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLT
PEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILIL
RICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKV
YVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPS

--------------------------------------------------------------

>59987_59987_14_NPM1-RARA_NPM1_chr5_170818803_ENST00000517671_RARA_chr17_38504565_ENST00000394089_length(amino acids)=551AA_BP=148
MAPKLRKDKDFGDVFSGRTELKNKVPACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHI
VEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVC
QDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLT
PEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILIL
RICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKV
YVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPS

--------------------------------------------------------------

>59987_59987_15_NPM1-RARA_NPM1_chr5_170818803_ENST00000517671_RARA_chr17_38504567_ENST00000254066_length(amino acids)=551AA_BP=148
MAPKLRKDKDFGDVFSGRTELKNKVPACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHI
VEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVC
QDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLT
PEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILIL
RICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKV
YVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPS

--------------------------------------------------------------

>59987_59987_16_NPM1-RARA_NPM1_chr5_170818803_ENST00000517671_RARA_chr17_38504567_ENST00000394089_length(amino acids)=551AA_BP=148
MAPKLRKDKDFGDVFSGRTELKNKVPACRHPMEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHI
VEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSSSSEEIVPSPPSPPPLPRIYKPCFVC
QDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEVPKPECSESYTLT
PEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILIL
RICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKV
YVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPS

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:170818803/chr17:38504565)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NPM1 P06748	RARA P10276
FUNCTION: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029). {ECO:0000269\|PubMed:12882984, ECO:0000269\|PubMed:16107701, ECO:0000269\|PubMed:17015463, ECO:0000269\|PubMed:18809582, ECO:0000269\|PubMed:19188445, ECO:0000269\|PubMed:20352051, ECO:0000269\|PubMed:21084279, ECO:0000269\|PubMed:22002061, ECO:0000269\|PubMed:22528486, ECO:0000269\|PubMed:25956029}.	FUNCTION: Receptor for retinoic acid (PubMed:19850744, PubMed:16417524, PubMed:20215566). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PubMed:28167758). The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (PubMed:28167758, PubMed:19398580). In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression (PubMed:16417524). On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation (PubMed:9267036, PubMed:19850744, PubMed:20215566). Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758). Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758). RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis (By similarity). Has a role in the survival of early spermatocytes at the beginning prophase of meiosis (By similarity). In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes (By similarity). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Together with RXRA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells (PubMed:28167758). In association with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and thereby promotes the inflammatory response (PubMed:28167758). {ECO:0000250\|UniProtKB:P11416, ECO:0000269\|PubMed:16417524, ECO:0000269\|PubMed:19398580, ECO:0000269\|PubMed:19850744, ECO:0000269\|PubMed:20215566, ECO:0000269\|PubMed:28167758, ECO:0000269\|PubMed:9267036}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000296930

1_9

117.33333333333333

295.0

Compositional bias

Note=Met-rich

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000351986

1_9

117.33333333333333

266.0

Compositional bias

Note=Met-rich

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000393820

1_9

117.33333333333333

260.0

Compositional bias

Note=Met-rich

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000517671

1_9

117.33333333333333

295.0

Compositional bias

Note=Met-rich

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

88_153

59.333333333333336

463.0

DNA binding

Nuclear receptor

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

88_153

54.333333333333336

458.0

DNA binding

Nuclear receptor

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

88_153

59.333333333333336

463.0

DNA binding

Nuclear receptor

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

88_153

366.0

DNA binding

Nuclear receptor

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

183_417

59.333333333333336

463.0

Domain

NR LBD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

183_417

54.333333333333336

458.0

Domain

NR LBD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

183_417

59.333333333333336

463.0

Domain

NR LBD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

183_417

366.0

Domain

NR LBD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

408_416

59.333333333333336

463.0

Motif

9aaTAD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

408_416

54.333333333333336

458.0

Motif

9aaTAD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

408_416

59.333333333333336

463.0

Motif

9aaTAD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

408_416

366.0

Motif

9aaTAD

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

154_182

59.333333333333336

463.0

Region

Note=Hinge

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

404_419

59.333333333333336

463.0

Region

Note=Required for binding corepressor NCOR1

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

154_182

54.333333333333336

458.0

Region

Note=Hinge

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

404_419

54.333333333333336

458.0

Region

Note=Required for binding corepressor NCOR1

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

154_182

59.333333333333336

463.0

Region

Note=Hinge

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

404_419

59.333333333333336

463.0

Region

Note=Required for binding corepressor NCOR1

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

154_182

366.0

Region

Note=Hinge

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

1_87

366.0

Region

Note=Modulating

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

404_419

366.0

Region

Note=Required for binding corepressor NCOR1

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

124_148

59.333333333333336

463.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

88_108

59.333333333333336

463.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

124_148

54.333333333333336

458.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

88_108

54.333333333333336

458.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

124_148

59.333333333333336

463.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

88_108

59.333333333333336

463.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

124_148

366.0

Zinc finger

NR C4-type

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000425707

88_108

366.0

Zinc finger

NR C4-type

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000296930

120_132

117.33333333333333

295.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000296930

161_188

117.33333333333333

295.0

Compositional bias

Note=Asp/Glu-rich (highly acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000351986

120_132

117.33333333333333

266.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000351986

161_188

117.33333333333333

266.0

Compositional bias

Note=Asp/Glu-rich (highly acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000393820

120_132

117.33333333333333

260.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000393820

161_188

117.33333333333333

260.0

Compositional bias

Note=Asp/Glu-rich (highly acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000517671

120_132

117.33333333333333

295.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000517671

161_188

117.33333333333333

295.0

Compositional bias

Note=Asp/Glu-rich (highly acidic)

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000296930

152_157

117.33333333333333

295.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000296930

191_197

117.33333333333333

295.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000351986

152_157

117.33333333333333

266.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000351986

191_197

117.33333333333333

266.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000393820

152_157

117.33333333333333

260.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000393820

191_197

117.33333333333333

260.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000517671

152_157

117.33333333333333

295.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000517671

191_197

117.33333333333333

295.0

Motif

Nuclear localization signal

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000296930

243_294

117.33333333333333

295.0

Region

Note=Required for nucleolar localization

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000351986

243_294

117.33333333333333

266.0

Region

Note=Required for nucleolar localization

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000393820

243_294

117.33333333333333

260.0

Region

Note=Required for nucleolar localization

Hgene

NPM1

chr5:170818803

chr17:38504565

ENST00000517671

243_294

117.33333333333333

295.0

Region

Note=Required for nucleolar localization

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000254066

1_87

59.333333333333336

463.0

Region

Note=Modulating

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394081

1_87

54.333333333333336

458.0

Region

Note=Modulating

Tgene

RARA

chr5:170818803

chr17:38504565

ENST00000394089

1_87

59.333333333333336

463.0

Region

Note=Modulating

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)

PDB file (386) >>>386.pdbFusion protein BP residue: 143 CIF file (386) >>>386.cif	NPM1	chr5	170818803	+	RARA	chr17	38504567	+	MRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCELK ADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKV TLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQSS SSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSI QKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKK KEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQ RVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACL DILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQL LPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKR RPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLEN	546
3D view using mol* of 386 (AA BP:143)


PDB file (395) >>>395.pdbFusion protein BP residue: 148 CIF file (395) >>>395.cif	NPM1	chr5	170818803	+	RARA	chr17	38504567	+	MAPKLRKDKDFGDVFSGRTELKNKVPACRHPMEDSMDMDMSPLRPQNYLF GCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEG SPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAI ETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGF FRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRND RNKKKKEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTN NSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLL KAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFA FANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKV YVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQ EMLENSEGLDTLSGQPGGGGRDGGGLAPPPGSCSPSLSPSSNRSSPATHS	551
3D view using mol* of 395 (AA BP:148)


PDB file (469) >>>469.pdbFusion protein BP residue: 194 CIF file (469) >>>469.cif	NPM1	chr5	170818803	+	RARA	chr17	38504567	+	MKSACAGRLRYGGGRASEHARGGGTWEALARSSGSIYKRGEPASFPWCDS VLRGCSLEQRSFISVRLLSYLSACRHPMEDSMDMDMSPLRPQNYLFGCEL KADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIK VTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAIETQS SSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRS IQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKK KKEVPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSE QRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAAC LDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQ LLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRK RRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLE	597
3D view using mol* of 469 (AA BP:194)

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NPM1_pLDDT.png

RARA_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NPM1_RARA_386_pLDDT.png (AA BP:143)

NPM1_RARA_386_pLDDT_and_active_sites.png (AA BP:143)

NPM1_RARA_386_violinplot.png (AA BP:143)

NPM1_RARA_395_pLDDT.png (AA BP:148)

NPM1_RARA_395_pLDDT_and_active_sites.png (AA BP:148)

NPM1_RARA_395_violinplot.png (AA BP:148)

NPM1_RARA_469_pLDDT.png (AA BP:194)

NPM1_RARA_469_pLDDT_and_active_sites.png (AA BP:194)

NPM1_RARA_469_violinplot.png (AA BP:194)

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

NPM1_RARA_386.png

NPM1_RARA_395.png

NPM1_RARA_469.png

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Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Fusion AA seq ID in FusionPDB	Site score	Size	D score	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
386	1.314	141	1.408	166.012	0.242	0.968	1.261	4.553	0.347	13.123	1.341	Chain A: 283,309,312,313,315,316,318,319,350,353,3 54,356,357,360,369,370,371,385,386,389,475,478,479 ,482,490,494,498
395	0.932	289	1.068	116.62	0.866	0.392	0.288	0.229	0.329	0.696	1.718	Chain A: 43,45,47,49,51,52,70,72,74,76,77,78,79,80 ,88,107,108,109,110,111,114,115,116,117,118,119,12 0,121,122,123,124,141,142,143,144,145,147,148,149, 461,464,465,467,468
469	1.234	149	1.3255	225.351	0.3713	0.8679	1.1508	3.5081	0.4151	8.4522	1.1663	Chain A: 333,334,335,360,363,366,367,369,370,372,3 73,401,404,405,407,408,411,421,422,436,437,440,444 ,526,529,530,533,541,545,549

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Potentially Interacting Small Molecules through Virtual Screening

The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.

Fusion AA seq ID in FusionPDB	ZINC ID	DrugBank ID	Drug name	Docking score	Glide gscore
386	ZINC000000121541	DB00643	Mebendazole	-10.051	-10.7092
386	ZINC000000968328	DB00412	Rosiglitazone	-9.03752	-10.372
386	ZINC000001552174	DB01166	Cilostazol	-10.0524	-10.0524
386	ZINC000007997897	DB00735	Naftifine	-9.73581	-9.81531
386	ZINC000000018635	DB00998	Frovatriptan	-9.63682	-9.63732
386	ZINC000006467621	DB11115	Ensulizole	-9.0783	-9.4892
386	ZINC000001530977	DB00735	Naftifine	-9.39614	-9.47564
386	ZINC000000968327	DB01132	Pioglitazone	-7.54035	-9.45865
386	ZINC000035653009	DB06209	Prasugrel	-8.74098	-9.42098
386	ZINC000252679615	DB01219	Dantrolene	-9.33732	-9.40972
386	ZINC000007997966	DB01219	Dantrolene	-9.33732	-9.40972
386	ZINC000052716421	DB04908	Flibanserin	-9.37958	-9.39048
386	ZINC000000403010	DB00598	Labetalol	-9.34547	-9.37307
386	ZINC000035653007	DB06209	Prasugrel	-8.66475	-9.34475
386	ZINC000000527386	DB00715	Paroxetine	-9.32635	-9.32745
386	ZINC000000000416	DB00598	Labetalol	-9.23318	-9.26078
386	ZINC000000005423	DB04854	Febuxostat	-9.24854	-9.24894
386	ZINC000000968310	DB00370	Mirtazapine	-9.11081	-9.19891
386	ZINC000004693575	DB00338	Omeprazole	-9.16255	-9.19655
386	ZINC000004693574	DB00338	Omeprazole	-7.3964	-9.1066

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Drug information from DrugBank of the top 20 interacting small molecules.

ZINC ID	DrugBank ID	Drug name	Drug type	SMILES	Drug group
ZINC000000121541	DB00643	Mebendazole	Small molecule	COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C1=CC=CC=C1	Approved\|Vet_approved
ZINC000001552174	DB01166	Cilostazol	Small molecule	O=C1CCC2=C(N1)C=CC(OCCCCC1=NN=NN1C1CCCCC1)=C2	Approved\|Investigational
ZINC000007997897	DB00735	Naftifine	Small molecule	CN(CC=CC1=CC=CC=C1)CC1=CC=CC2=CC=CC=C12	Approved
ZINC000000018635	DB00998	Frovatriptan	Small molecule	CN[C@@H]1CCC2=C(C1)C1=C(N2)C=CC(=C1)C(N)=O	Approved\|Investigational
ZINC000006467621	DB11115	Ensulizole	Small molecule	OS(=O)(=O)C1=CC2=C(C=C1)N=C(N2)C1=CC=CC=C1	Approved
ZINC000000968327	DB01132	Pioglitazone	Small molecule	CCC1=CN=C(CCOC2=CC=C(CC3SC(=O)NC3=O)C=C2)C=C1	Approved\|Investigational
ZINC000035653009	DB06209	Prasugrel	Small molecule	CC(=O)OC1=CC2=C(CCN(C2)C(C(=O)C2CC2)C2=CC=CC=C2F)S1	Approved
ZINC000007997966	DB01219	Dantrolene	Small molecule	[O-][N+](=O)C1=CC=C(C=C1)C1=CC=C(O1)C=NN1CC(=O)NC1=O	Approved\|Investigational
ZINC000052716421	DB04908	Flibanserin	Small molecule	FC(F)(F)C1=CC(=CC=C1)N1CCN(CCN2C(=O)NC3=CC=CC=C23)CC1	Approved\|Investigational
ZINC000000527386	DB00715	Paroxetine	Small molecule	FC1=CC=C(C=C1)[C@@H]1CCNC[C@H]1COC1=CC2=C(OCO2)C=C1	Approved\|Investigational
ZINC000000005423	DB04854	Febuxostat	Small molecule	CC(C)COC1=C(C=C(C=C1)C1=NC(C)=C(S1)C(O)=O)C#N	Approved
ZINC000004693574	DB00338	Omeprazole	Small molecule	COC1=CC2=C(C=C1)N=C(N2)S(=O)CC1=NC=C(C)C(OC)=C1C	Approved\|Investigational\|Vet_approved

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Biochemical Features of Small Molecules

ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)

ZINC ID	mol_MW	dipole	SASA	FOSA	FISA	PISA	WPSA	volume	donorHB	accptHB	IP	Human Oral Absorption	Percent Human Oral Absorption	Rule Of Five	Rule Of Three
ZINC000000121541	295.297	6.562	576.161	98.468	169.387	308.306	0	957.18	1	5	8.911	3	83.641	0	0
ZINC000000121541	295.297	2.873	577.033	96.773	172.83	307.43	0	959.022	0	3	8.668	3	88.187	0	0
ZINC000000121541	295.297	10.919	575.199	98.469	170.072	306.658	0	956.419	1	5	8.861	3	83.461	0	0
ZINC000000968328	357.426	1.444	634.074	175.676	137.918	287.735	32.745	1113.731	1	5.25	8.373	3	96.012	0	0
ZINC000000968328	357.426	2.563	632.369	175.708	136.258	287.719	32.684	1111.853	1	5.25	8.348	3	96.294	0	0
ZINC000000968328	357.426	1.098	615.115	188.498	120.189	271.15	35.279	1101.068	1	5.25	8.417	3	100	0	0
ZINC000000968328	357.426	4.059	637.846	177.05	121.495	296.234	43.067	1111.876	1	5.25	8.521	3	100	0	0
ZINC000001552174	369.466	5.493	671.338	413.118	163.279	94.942	0	1212.429	1	6.25	8.761	3	89.813	0	0
ZINC000007997897	287.404	0.789	607.9	141.903	0.103	465.895	0	1056.595	0	2	8.523	3	100	1	0
ZINC000007997897	287.404	0.718	598.565	138.957	0	459.608	0	1048.049	0	2	8.531	3	100	1	0
ZINC000007997897	287.404	0.791	521.98	157.971	2.38	361.628	0	973.251	0	2	8.498	3	100	0	0
ZINC000000018635	243.308	7.2	492.762	217.467	154.534	120.761	0	833.223	4	4	8.296	3	66.41	0	0
ZINC000006467621	274.294	9.514	495.179	0	165.642	327.077	2.46	816.857	2	5.5	9.09	3	70.241	0	0
ZINC000006467621	274.294	5.937	496.167	0	165.586	328.124	2.456	817.805	2	5.5	9.121	3	70.288	0	0
ZINC000006467621	274.294	9.57	496.894	0	166.022	328.377	2.495	819.646	2	5.5	8.954	3	70.264	0	0
ZINC000001530977	287.404	0.712	605.454	136.509	0	468.945	0	1057.693	0	2	8.528	3	100	1	0
ZINC000001530977	287.404	0.785	611.686	137.629	0	474.057	0	1063.267	0	2	8.507	3	100	1	0
ZINC000001530977	287.404	0.834	689.787	145.585	0.993	543.21	0	1132.152	0	2	8.488	1	100	1	1
ZINC000000968327	356.439	3.797	649.705	257.697	124.274	224.356	43.379	1140.406	1	4.75	9.075	3	100	0	0
ZINC000000968327	356.439	2.594	661.839	250.138	139.459	239.977	32.265	1145.281	1	4.75	9.184	3	100	0	0
ZINC000000968327	356.439	2.857	652.728	259.013	125.786	224.546	43.384	1142.385	1	4.75	9.131	3	100	0	0
ZINC000000968327	356.439	3.362	661.519	250.269	140.099	238.95	32.2	1145.298	1	4.75	9.214	3	100	0	0
ZINC000035653009	373.441	6.105	631.829	306.899	73.023	179.017	72.891	1149.824	0	6.5	9.028	3	93.348	0	0
ZINC000035653009	373.441	3.947	664.809	332.695	70.28	185.403	76.43	1173.31	0	6.5	9.032	3	94.944	0	0
ZINC000035653009	373.441	1.874	665.065	333.533	69.091	186.237	76.204	1172.531	0	6.5	9.053	3	95.165	0	0
ZINC000252679615	314.257	3.867	548.758	54.323	243.185	251.25	0	924.46	1	5	9.205	3	67.058	0	0
ZINC000252679615	314.257	2.557	527.996	64.383	238.233	225.38	0	905.494	1	5	9.686	3	67.506	0	0
ZINC000252679615	314.257	4.186	547.3	54.729	241.127	251.444	0	922.097	1	5	9.191	3	67.408	0	0
ZINC000252679615	314.257	2.845	525.884	65.15	235.939	224.795	0	902.055	1	5	9.671	3	67.875	0	0
ZINC000007997966	314.257	2.557	527.996	64.383	238.233	225.38	0	905.494	1	5	9.686	3	67.506	0	0
ZINC000007997966	314.257	2.845	525.884	65.15	235.939	224.795	0	902.055	1	5	9.671	3	67.875	0	0
ZINC000052716421	390.407	5.142	640.176	175.082	77.552	270.36	117.181	1153.769	1	5	8.586	3	100	0	0
ZINC000052716421	390.407	5.676	638.499	175.573	83.935	263.406	115.586	1157.43	1	5	8.626	3	100	0	0
ZINC000000403010	328.41	5.633	665.048	169.772	199.786	295.49	0	1141.271	4	5.45	9.068	2	70.572	0	0
ZINC000000403010	328.41	8.153	662.671	168.348	200.011	294.312	0	1140.633	4	5.45	9.267	2	70.521	0	0
ZINC000035653007	373.441	5.528	683.981	366.022	68.884	174.527	74.548	1197.181	0	6.5	9.264	3	96.033	0	0
ZINC000035653007	373.441	7.4	663.183	335.264	67.351	199.174	61.394	1171.3	0	6.5	9.112	3	95.242	0	0
ZINC000035653007	373.441	7.914	660.593	336.74	66.06	194.116	63.676	1170.488	0	6.5	9.12	3	95.499	0	0
ZINC000000527386	329.37	3.474	566.298	235.45	29.534	254.5	46.814	1013.808	1	3.75	8.771	3	100	0	0
ZINC000000000416	328.41	5.718	665.292	169.819	199.85	295.623	0	1140.603	4	5.45	9.123	2	70.547	0	0
ZINC000000000416	328.41	6.318	651.677	168.706	200.025	282.946	0	1135.812	4	5.45	9.228	2	70.416	0	0
ZINC000000005423	316.374	4.229	610.22	277.046	172.419	128.649	32.105	1039.03	1	5.75	9.355	3	76.016	0	0
ZINC000000968310	265.357	2.146	504.113	215.256	13.985	274.872	0	890.178	0	3.5	8.524	3	100	0	0
ZINC000000968310	265.357	1.467	512.402	223.811	10.787	277.804	0	899.239	0	3.5	8.602	3	100	0	0
ZINC000000968310	265.357	2.159	506.854	212.362	17.71	276.782	0	897.097	0	3.5	8.319	3	100	0	0
ZINC000000968310	265.357	1.212	507.134	222.435	12.709	271.99	0	897.117	0	3.5	8.473	3	100	0	0
ZINC000004693575	345.415	9.429	623.225	328.34	88.021	192.896	13.968	1086.117	1	8	8.845	2	71.533	0	1
ZINC000004693575	345.415	4.914	587.268	328.185	83.135	160.536	15.412	1061.202	1	8	8.713	2	72.366	0	1
ZINC000004693574	345.415	1.818	611.368	328.475	76.302	194.526	12.065	1078.902	1	8	8.789	2	73.54	0	1
ZINC000004693574	345.415	1.805	605.215	321.035	80.627	192.587	10.965	1078.319	1	8	8.8	2	72.79	0	1

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Drug Toxicity Information

Toxicity information of individual drugs using eToxPred

ZINC ID	Smile	Surface Accessibility	Toxicity
ZINC000000121541	COC(=O)/N=c1[nH]c2ccc(C(=O)c3ccccc3)cc2[nH]1	0.209696342	0.592445172
ZINC000000968328	CN(CCOc1ccc(C[C@@H]2SC(=O)NC2=O)cc1)c1ccccn1	0.155326871	0.273380926
ZINC000001552174	O=C1CCc2cc(OCCCCc3nnnn3C3CCCCC3)ccc2N1	0.221212704	0.308586478
ZINC000007997897	CN(C/C=Cc1ccccc1)Cc1cccc2ccccc12	0.394772836	0.303491673
ZINC000000018635	CN[C@@H]1CCc2[nH]c3ccc(C(N)=O)cc3c2C1	0.15678731	0.476568578
ZINC000006467621	O=S(=O)(O)c1ccc2[nH]c(-c3ccccc3)nc2c1	0.38504104	0.405031793
ZINC000001530977	CN(C/C=C/c1ccccc1)Cc1cccc2ccccc12	0.394772836	0.303491673
ZINC000000968327	CCc1ccc(CCOc2ccc(C[C@H]3SC(=O)NC3=O)cc2)nc1	0.162255791	0.381863632
ZINC000035653009	CC(=O)Oc1cc2c(s1)CCN([C@@H](C(=O)C1CC1)c1ccccc1F)C2	0.108110837	0.299041325
ZINC000252679615	O=C1CN(N=Cc2ccc(-c3ccc([N+](=O)[O-])cc3)o2)C(O)=N1	0.149132109	0.500589886
ZINC000007997966	O=C1CN(/N=Cc2ccc(-c3ccc([N+](=O)[O-])cc3)o2)C(O)=N1	0.149132109	0.500589886
ZINC000052716421	O=c1[nH]c2ccccc2n1CCN1CCN(c2cccc(C(F)(F)F)c2)CC1	0.300121372	0.137722381
ZINC000000403010	C[C@@H](CCc1ccccc1)NC[C@H](O)c1ccc(O)c(C(N)=O)c1	0.163888793	0.349082082
ZINC000035653007	CC(=O)Oc1cc2c(s1)CCN([C@H](C(=O)C1CC1)c1ccccc1F)C2	0.108110837	0.299041325
ZINC000000527386	Fc1ccc([C@@H]2CCNC[C@H]2COc2ccc3c(c2)OCO3)cc1	0.13727164	0.457658294
ZINC000000000416	C[C@H](CCc1ccccc1)NC[C@@H](O)c1ccc(O)c(C(N)=O)c1	0.163888793	0.349082082
ZINC000000005423	Cc1nc(-c2ccc(OCC(C)C)c(C#N)c2)sc1C(=O)O	0.265945843	0.274809601
ZINC000000968310	CN1CCN2c3ncccc3Cc3ccccc3[C@H]2C1	0.161648552	0.278705347
ZINC000004693575	COc1ccc2nc([S@@](=O)Cc3ncc(C)c(OC)c3C)[nH]c2c1	0.130122236	0.350645543
ZINC000004693574	COc1ccc2nc([S@](=O)Cc3ncc(C)c(OC)c3C)[nH]c2c1	0.130122236	0.350645543

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

NPM1

TCERG1, LYAR, USF2, HIST3H3, SWAP70, HAND2, NCL, OTUB1, AKT1, CDT1, RRP1B, Rrp1b, GRB2, CENPF, CASKIN1, CDC14A, PIK3R1, NUP98, PPID, GRB7, NCAPG, NUMA1, NSUN2, PADI4, TP53, BARD1, BRCA1, H2AFX, CDKN2A, HMGA2, HMGA1, HCVgp1, YY1, HIST1H3A, HIST2H2AC, HIST2H2BE, HIST1H4A, EP300, tat, PML, RARA, CTNNBL1, FANCA, FANCC, TFAP2A, HDAC1, HDAC2, SMARCA4, DOT1L, ACACA, HNRNPM, HNRNPU, DDX21, HIST1H1C, SIRT1, YBX1, PC, YBX3, H1FX, HIST1H1A, ESR1, Trp53, Cdkn2a, CDK5RAP3, ZNF668, UBC, RYK, SMN1, CD4, YWHAQ, USP36, CTCF, HDAC5, CDKN1A, NOP56, CENPA, AFF1, DYRK2, TOP1, CDK2, SREK1, SENP3, RB1, PARP1, HJURP, ARRB1, ARRB2, SIRT7, NPM3, NPM1, FBXO25, IRF1, CUL3, CUL4A, CUL5, CUL2, CUL1, COPS5, COPS6, DCUN1D1, CAND1, MDM2, PA2G4, PPP2R1A, MID1, GZF1, APEX1, COPS2, PLCG1, FBXO6, GAPDH, SIAH1, PTBP1, SRSF1, APP, RPL14, RPS6, RPL24, RPL6, NAP1L1, HNRNPR, RPS4X, PRPF6, LAMP2, SURF4, RPL10L, NAP1L4, ZYX, NUP62, NUP50, RPL5, YWHAE, FMNL1, CPSF6, HNRNPH1, PAPOLG, JUNB, MC4R, HIVEP2, CPSF1, CPSF2, CPSF4, CPSF3, FN1, VCAM1, CSNK2A1, NOS2, UBL4A, ITGA4, CBX2, CBX4, CBX8, PHB, EIF2AK2, XPO1, GZMM, PAN2, CD81, IGSF8, ICAM1, PRKCZ, KIF11, SQSTM1, FLNA, LLGL1, HSP90AA1, TRIM21, TUBA1C, HSP90AB1, PARD6B, CDC37, TUBB3, MRPL46, KCTD2, CLTC, MRPL50, VCP, KCTD5, MRPL12, HSPA5, PTRF, MRPL52, EEF1A1, KCTD17, TAB1, BAG2, ANXA2, EIF5A, PPIL4, MRPL38, STK38, FLNB, LGALS1, C1QBP, COPB1, MRPL37, MRPL49, KEAP1, PRDX4, WDR26, PSMC4, SDPR, SLC25A3, RPS12, EEF1B2, NIPSNAP1, EEF1G, MRPL39, DDX3X, RNH1, OSBPL1A, EIF3H, MRPL54, ENO1, MYCBP, MRPL18, MAP1B, PSMD1, EEF1D, DNAJC13, HSPB1, CFL1, FLNC, PPP1CC, RPLP1, CEP152, SNRPD1, ATP5A1, ARHGAP5, PRKAR1A, MRPL53, GID8, YWHAZ, CCT2, EIF3E, LAMB2, DICER1, CHCHD4, MRPL19, SNRPD2, GRWD1, TPR, PSMD4, PSMC5, PPP2CA, UNC5C, SLC25A5, PTPN14, RPS5, TTC27, BOLA2, EIF4A1, TUFM, S100A10, IMPG1, NUDT21, UTRN, MRPL44, EIF3M, MRPL43, PKM, IMPDH2, TRRAP, CHCHD6, RANBP6, EIF3I, TAB2, MRPL24, DZIP1, IPO5, AGR2, MRPS30, ICT1, PRDX6, TCP1, RCOR1, GRN, TXN, RPLP0, PCBP1, MRPL28, MRPL10, TAF3, EPAS1, CHCHD3, TXNDC12, ELMO2, SRRT, MRPL11, EIF2S1, SNRPF, KTN1, FGD6, UQCRH, RBBP4, DNAJA1, CTTN, USP15, NUDCD1, RPS28, JAK1, PROS1, PYGB, KDR, EEF2, FASN, AKAP11, CSNK2B, DNAJA2, EIF3G, DHX15, MRPS26, HNRNPA0, DOCK4, PRKCI, VASP, COPE, SAMM50, TEX15, PSME3, PPM1G, COPG1, CALU, S100A11, HSPA4, PSMD12, POLR2E, TRIM28, SLAIN2, RPL10, GOLGA2, GOLGA3, PRKCA, IPO9, PARK7, SPIN1, PPA1, BANP, DSTN, GNAI2, HOXA7, ABCC1, RELA, ACY1, TARDBP, PARK2, KPNA1, ARMCX3, LIMCH1, OSBP, TRMT61A, rev, RPA1, RPA2, RPA3, ERG, LGR4, STAU1, ELF4, AURKA, HUWE1, FUS, COX8A, NPM2, MOV10, NXF1, PHF6, CUL7, OBSL1, CCDC8, SIRT6, EBNA1BP2, NOL12, RPL10A, POP4, ZNF22, NSA2, TAF1D, NIFK, RPL26L1, NIP7, RPL4, RPF1, CCDC137, KNOP1, RBM28, RPL7A, POP1, DDX24, FTSJ3, RRS1, RPL3, ZFP62, ZNF512, DDX56, DDX27, GLYR1, MAK16, CEBPZ, RSL1D1, HP1BP3, REXO4, DDX31, MYBBP1A, NOP16, C3orf17, RRP8, GNL2, PAPD5, RSL24D1, SURF6, GTPBP4, KIAA0020, NOP2, NVL, GLTSCR2, URB1, RPL23A, GPATCH4, TEX10, RPS8, NOC3L, BRIX1, DDX54, PWP1, RBM34, NMNAT1, CENPC, SENP5, RPL36AL, PAK1IP1, ZNF800, RPL7L1, RPL37A, RPF2, PELP1, NOC2L, RPP40, RBMX2, CENPV, PPAN-P2RY11, SDAD1, RPP38, RPP25, SPTY2D1, RPP25L, POP7, RPS6KB2, UTP15, NTRK1, EWSR1, CLK1, HIST1H3E, BYSL, CHD1, DDX1, DECR1, EIF1AX, EIF4G2, FBL, FAU, HIST1H1D, HIST1H1B, RPSA, MKI67, MPG, EXOSC10, RPL7, RPL8, RPL9, RPL11, RPL13, RPL15, RPL17, RPL18, RPL18A, RPL19, RPL21, RPL22, RPL27, RPL27A, RPL29, RPL32, RPL37, RPL38, RPS2, RPS3, RPS3A, RPS9, RPS10, RPS11, RPS13, RPS14, RPS15, RPS16, RPS17, RPS18, RPS19, RPS20, RPS21, RPS23, RPS24, RPS25, RPS27, RPS29, SKIV2L, SRP72, XPC, IFRD2, HIST1H2BC, SMARCA5, CGGBP1, EIF3A, EIF3C, PABPC4, USP10, TTC37, JADE3, ABCF2, G3BP1, GNB2L1, EMG1, IGF2BP3, PSIP1, SUPT16H, RPL35, PDCD11, LARP4B, RRP12, LARP1, RPL13A, RPL36, MTHFD1L, AHCTF1, FAM98A, SERBP1, GNL3, SND1, NOB1, RPS27L, DDX47, EIF3L, RTCB, TRMT112, DHX29, MTPAP, NAT10, TSR1, NKRF, MEPCE, BCCIP, PNO1, MRPS22, WDR18, SCAF1, NOL6, DDX50, CCDC86, MUS81, ZNF622, DHX57, H2AFV, LARP4, WBSCR22, Eif3a, Eif3e, Ktn1, Rpl35, Srp72, Rrbp1, GAN, HEMGN, TMUB1, CRY1, DNAJB4, MCM2, GLI1, Mdm2, SP1, U2AF2, RC3H1, EIF2S2, EGFR, WWP2, CD24, ZNF746, DDX51, MAGED2, HNRNPA2B1, HNRNPD, HNRNPA1, RPL23, RPS7, HIST1H2BB, HIST1H2AA, CBX3, CYLD, INO80B, LMNA, YAP1, MTF1, BRD1, HDAC6, FBXW7, CENPW, API5, CTNNB1, MAP2K3, BMP4, CCNT1, DIMT1, MATN2, TRIP4, CUL4B, UBE2M, PRPF8, EFTUD2, AAR2, PIH1D1, CHD3, RNF4, CHD4, LARP7, RNF31, TNF, FAM188B, SPDL1, HEXIM1, SNAI1, RECQL4, GPC1, REST, ZFP36L2, MYC, CDK9, Prkab1, NEK2, METTL3, METTL14, KIAA1429, RC3H2, PSMA3, ACTC1, ESR2, FAF1, RBX1, BRCA2, DISC1, NR2C2, UBQLN2, MTDH, GADD45A, AGRN, ATXN3, VRK1, VRK3, DYRK1A, SNRNP70, ITFG1, GHET1, ARAF, HMGB1, BIRC3, NFX1, BRD7, N, SOX2, PPIA, CACYBP, RNR1, CMTR1, ARIH2, PLEKHA4, RAD18, PINK1, WHSC1, FANCD2, SAMD12, LINC01554, ZC3H18, CAMK2A, FYN, PTPN12, RPS6KA3, STX7, IL7R, GPC3, RP1, KMT2A, SUZ12, BSX, LEMD1, ZCCHC7, SYNE2, ZBTB9, R3HDM4, ZNF496, PIKFYVE, COL8A1, PMS2, TRDN, PLCZ1, ASPM, TTN, SETD2, BDP1, C4orf47, CFAP54, HIST2H2BC, AIM2, SYT7, LTN1, EPRS, FST, LMOD1, MCAM, RPL34, MYT1, DYNC1I2, KIF22, ZNF181, ATAT1, TRMT10B, RBM44, ANKS3, FILIP1, FAM9A, SUPT20H, THOC2, CENPI, BICD1, CCL13, JPH2, NDST4, SARDH, SLC27A6, LECT2, HMGN4, OPA1, ITGB1, HIVEP1, PNRC1, PDE4DIP, CAPRIN2, C1orf110, MICAL1, STAB2, CEP63, NGRN, CAMSAP3, ARHGEF9, SNIP1, ORF14, ILF3, LRRC31, DUX4, CIT, ANLN, AURKB, CHMP4B, CHMP4C, ECT2, KIF14, KIF20A, KIF23, PRC1, BRD3, SP110, TRIM24, ZMYND8, LRRC59, NMRAL1, SUMO2, Rnf183, BRD4, NUPR1, RBM45, CIC, Apc2, RBM39, FBP1, ASXL1, vpr, RIN3, DNAJC19, DNAJC25, DNAJC2, OGT, DDRGK1, UFL1, CD3EAP, DDX23, HIST1H2BG, ACTG1, ALKBH4, DNAJC10, GLUD2, HIST1H2AE, HIST1H3F, HIST1H4J, HLCS, HSPA1B, MALT1, PEG10, PLD3, UBB, AIP, BCORL1, BID, CSK, LCK, PSMG1, RAB21, RBM11, VDAC1, RPL31, ZNF330, TRIM37, FZR1, WDR5, PAGE4, NUDCD2, NAA40, BGLT3, CCDC140, RPL23AP32, MTG2, NGDN, UTP18, PRPF4B, WDR74, WDR36, DKC1, DDX52, EPB41L5, ZBTB11, H2AFY, CIRH1A, SRSF5, DUSP11, DDX10, TSPYL1, IMP3, RBM19, NOM1, ZC3HAV1, RPP14, UTP14A, WDR12, DNTTIP2, MPHOSPH10, RRP1, WDR3, BUD13, RPLP2, C14orf169, ZNF771, AATF, C1orf35, OASL, NLE1, BMS1, BOP1, STAU2, TAF1C, WDR55, ZNF638, RFC1, FYTTD1, WDR43, C7orf50, SRPK2, LUC7L, FAM111A, PBRM1, NOL10, TAF1A, TBL3, ZNF770, BAZ1B, ESF1, TTF1, LAS1L, RPP30, ZNF16, RRP15, ABT1, SPRTN, ULK1, TRAF6, BTF3, SLFN11, NLRP7, RCHY1, DIDO1, CCNF, FAM129A, ATR, ATM, PSMD9, ZEB1,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NPM1
RARA

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to NPM1-RARA

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene	Tgene	Drug	Source	PMID
NPM1	RARA	Steroids And Radiation	PubMed	26342691

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Related Diseases to NPM1-RARA

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene	Tgene	Disease	Source	PMID
NPM1	RARA	Acute Promyelocytic Leukemia (Apl)	PubMed	26342691
NPM1	RARA	Invasive Breast Carcinoma	MyCancerGenome
NPM1	RARA	Lung Adenocarcinoma	MyCancerGenome
NPM1	RARA	Breast Invasive Ductal Carcinoma	MyCancerGenome
NPM1	RARA	Esophageal Adenocarcinoma	MyCancerGenome
NPM1	RARA	Acute Myeloid Leukemia	MyCancerGenome

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	NPM1	C0026998	Acute Myeloid Leukemia, M1	6	CTD_human;ORPHANET
Hgene	NPM1	C1879321	Acute Myeloid Leukemia (AML-M2)	6	CTD_human;ORPHANET
Hgene	NPM1	C0023467	Leukemia, Myelocytic, Acute	5	CGI;CTD_human
Hgene	NPM1	C0023487	Acute Promyelocytic Leukemia	2	CGI;CTD_human;ORPHANET
Hgene	NPM1	C0024623	Malignant neoplasm of stomach	1	CTD_human
Hgene	NPM1	C0038356	Stomach Neoplasms	1	CTD_human
Hgene	NPM1	C0152013	Adenocarcinoma of lung (disorder)	1	CTD_human
Hgene	NPM1	C0206182	Lymphomatoid Papulosis	1	ORPHANET
Hgene	NPM1	C0265965	Dyskeratosis Congenita	1	CTD_human;GENOMICS_ENGLAND
Hgene	NPM1	C1148551	X-Linked Dyskeratosis Congenita	1	CTD_human
Hgene	NPM1	C1301362	Primary Cutaneous Anaplastic Large Cell Lymphoma	1	ORPHANET
Hgene	NPM1	C1708349	Hereditary Diffuse Gastric Cancer	1	CTD_human
Hgene	NPM1	C2930974	Acute erythroleukemia	1	CTD_human
Hgene	NPM1	C2930975	Acute erythroleukemia - M6a subtype	1	CTD_human
Hgene	NPM1	C2930976	Acute myeloid leukemia FAB-M6	1	CTD_human
Hgene	NPM1	C2930977	Acute erythroleukemia - M6b subtype	1	CTD_human
Tgene	RARA	C0023487	Acute Promyelocytic Leukemia	24	CTD_human;ORPHANET
Tgene	RARA	C0036341	Schizophrenia	3	PSYGENET
Tgene	RARA	C0006142	Malignant neoplasm of breast	1	CTD_human
Tgene	RARA	C0009363	Congenital ocular coloboma (disorder)	1	GENOMICS_ENGLAND
Tgene	RARA	C0010701	Phyllodes Tumor	1	CTD_human
Tgene	RARA	C0085183	Neoplasms, Second Primary	1	CTD_human
Tgene	RARA	C0086696	Neoplasms, Therapy-Associated	1	CTD_human
Tgene	RARA	C0149940	Sciatic Neuropathy	1	CTD_human
Tgene	RARA	C0154748	Lesion of Sciatic Nerve	1	CTD_human
Tgene	RARA	C0206650	Fibroadenoma	1	CTD_human
Tgene	RARA	C0242013	Sciatic Neuritis	1	CTD_human
Tgene	RARA	C0525045	Mood Disorders	1	PSYGENET
Tgene	RARA	C0600066	Malignant Cystosarcoma Phyllodes	1	CTD_human
Tgene	RARA	C0678222	Breast Carcinoma	1	CTD_human
Tgene	RARA	C0751924	Neuralgia-Neuritis, Sciatic Nerve	1	CTD_human
Tgene	RARA	C0751925	Sciatic Nerve Palsy	1	CTD_human
Tgene	RARA	C0877578	Treatment related secondary malignancy	1	CTD_human
Tgene	RARA	C1257931	Mammary Neoplasms, Human	1	CTD_human
Tgene	RARA	C1458155	Mammary Neoplasms	1	CTD_human
Tgene	RARA	C2239176	Liver carcinoma	1	CTD_human
Tgene	RARA	C4704874	Mammary Carcinoma, Human	1	CTD_human