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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NUCB1-RUVBL2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NUCB1-RUVBL2
	FusionPDB ID: 60762
	FusionGDB2.0 ID: 60762
		Hgene	Tgene
	Gene symbol	NUCB1	RUVBL2
	Gene ID	4924	10856
	Gene name	nucleobindin 1	RuvB like AAA ATPase 2
	Synonyms	CALNUC\|NUC	CGI-46\|ECP-51\|ECP51\|INO80J\|REPTIN\|RVB2\|TAP54-beta\|TIH2\|TIP48\|TIP49B
	Cytomap	19q13.33	19q13.33
	Type of gene	protein-coding	protein-coding
	Description	nucleobindin-1	ruvB-like 248 kDa TATA box-binding protein-interacting protein48 kDa TBP-interacting protein51 kDa erythrocyte cytosolic proteinINO80 complex subunit JRuvB (E coli homolog)-like 2TIP60-associated protein 54-betaerythrocyte cytosolic protein, 51-KD
	Modification date	20200313	20200327
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000263273, ENST00000405315, ENST00000407032, ENST00000485798,	ENST00000413176, ENST00000598768, ENST00000595090, ENST00000601968,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	9 X 11 X 6=594	6 X 7 X 6=252
	# samples	14	7
	** MAII score	log2(14/594*10)=-2.0850361038558 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/252*10)=-1.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NUCB1 [Title/Abstract] AND RUVBL2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NUCB1(49407711)-RUVBL2(49513033), # samples:3 RUVBL2(49502630)-NUCB1(49407604), # samples:1
Anticipated loss of major functional domain due to fusion event.	NUCB1-RUVBL2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NUCB1-RUVBL2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NUCB1-RUVBL2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NUCB1-RUVBL2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RUVBL2-NUCB1 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. RUVBL2-NUCB1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. RUVBL2-NUCB1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	RUVBL2	GO:0043967	histone H4 acetylation	14966270
Tgene	RUVBL2	GO:0043968	histone H2A acetylation	14966270
Tgene	RUVBL2	GO:0090090	negative regulation of canonical Wnt signaling pathway	11080158

Fusion gene breakpoints across NUCB1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across RUVBL2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LAML	TCGA-AB-2935-03A	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+
ChimerDB4	LAML	TCGA-AB-2935_61FFWAAXX_1	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+
ChimerDB4	LAML	TCGA-AB-2935_704G6AAXX_1	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+
ChimerDB4	LAML	TCGA-AB-2935	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000405315	NUCB1	chr19	49407711	+	ENST00000595090	RUVBL2	chr19	49513033	+	1660	577	334	1506	390
ENST00000405315	NUCB1	chr19	49407711	+	ENST00000601968	RUVBL2	chr19	49513033	+	1427	577	334	1314	326
ENST00000407032	NUCB1	chr19	49407711	+	ENST00000595090	RUVBL2	chr19	49513033	+	1390	307	64	1236	390
ENST00000407032	NUCB1	chr19	49407711	+	ENST00000601968	RUVBL2	chr19	49513033	+	1157	307	64	1044	326
ENST00000263273	NUCB1	chr19	49407711	+	ENST00000595090	RUVBL2	chr19	49513033	+	1339	256	13	1185	390
ENST00000263273	NUCB1	chr19	49407711	+	ENST00000601968	RUVBL2	chr19	49513033	+	1106	256	13	993	326
ENST00000405315	NUCB1	chr19	49407711	+	ENST00000595090	RUVBL2	chr19	49513032	+	1660	577	334	1506	390
ENST00000405315	NUCB1	chr19	49407711	+	ENST00000601968	RUVBL2	chr19	49513032	+	1427	577	334	1314	326
ENST00000407032	NUCB1	chr19	49407711	+	ENST00000595090	RUVBL2	chr19	49513032	+	1390	307	64	1236	390
ENST00000407032	NUCB1	chr19	49407711	+	ENST00000601968	RUVBL2	chr19	49513032	+	1157	307	64	1044	326
ENST00000263273	NUCB1	chr19	49407711	+	ENST00000595090	RUVBL2	chr19	49513032	+	1339	256	13	1185	390
ENST00000263273	NUCB1	chr19	49407711	+	ENST00000601968	RUVBL2	chr19	49513032	+	1106	256	13	993	326

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000405315	ENST00000595090	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+	0.003463187	0.99653685
ENST00000405315	ENST00000601968	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+	0.006584339	0.9934156
ENST00000407032	ENST00000595090	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+	0.003809983	0.99618995
ENST00000407032	ENST00000601968	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+	0.007274318	0.9927256
ENST00000263273	ENST00000595090	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+	0.004050227	0.99594975
ENST00000263273	ENST00000601968	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513033	+	0.007883003	0.992117
ENST00000405315	ENST00000595090	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+	0.003463187	0.99653685
ENST00000405315	ENST00000601968	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+	0.006584339	0.9934156
ENST00000407032	ENST00000595090	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+	0.003809983	0.99618995
ENST00000407032	ENST00000601968	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+	0.007274318	0.9927256
ENST00000263273	ENST00000595090	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+	0.004050227	0.99594975
ENST00000263273	ENST00000601968	NUCB1	chr19	49407711	+	RUVBL2	chr19	49513032	+	0.007883003	0.992117

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>60762_60762_1_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000263273_RUVBL2_chr19_49513032_ENST00000595090_length(amino acids)=390AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP
HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF

--------------------------------------------------------------

>60762_60762_2_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000263273_RUVBL2_chr19_49513032_ENST00000601968_length(amino acids)=326AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP

--------------------------------------------------------------

>60762_60762_3_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000263273_RUVBL2_chr19_49513033_ENST00000595090_length(amino acids)=390AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP
HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF

--------------------------------------------------------------

>60762_60762_4_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000263273_RUVBL2_chr19_49513033_ENST00000601968_length(amino acids)=326AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP

--------------------------------------------------------------

>60762_60762_5_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000405315_RUVBL2_chr19_49513032_ENST00000595090_length(amino acids)=390AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP
HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF

--------------------------------------------------------------

>60762_60762_6_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000405315_RUVBL2_chr19_49513032_ENST00000601968_length(amino acids)=326AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP

--------------------------------------------------------------

>60762_60762_7_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000405315_RUVBL2_chr19_49513033_ENST00000595090_length(amino acids)=390AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP
HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF

--------------------------------------------------------------

>60762_60762_8_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000405315_RUVBL2_chr19_49513033_ENST00000601968_length(amino acids)=326AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP

--------------------------------------------------------------

>60762_60762_9_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000407032_RUVBL2_chr19_49513032_ENST00000595090_length(amino acids)=390AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP
HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF

--------------------------------------------------------------

>60762_60762_10_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000407032_RUVBL2_chr19_49513032_ENST00000601968_length(amino acids)=326AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP

--------------------------------------------------------------

>60762_60762_11_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000407032_RUVBL2_chr19_49513033_ENST00000595090_length(amino acids)=390AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP
HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF

--------------------------------------------------------------

>60762_60762_12_NUCB1-RUVBL2_NUCB1_chr19_49407711_ENST00000407032_RUVBL2_chr19_49513033_ENST00000601968_length(amino acids)=326AA_BP=0
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL
KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR
TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:49407711/chr19:49513033)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

42_51

81.0

462.0

Region

Note=O-glycosylated at one site

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

42_51

81.0

462.0

Region

Note=O-glycosylated at one site

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

42_51

81.0

666.6666666666666

Region

Note=O-glycosylated at one site

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

42_51

81.0

462.0

Region

Note=O-glycosylated at one site

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

42_51

81.0

462.0

Region

Note=O-glycosylated at one site

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

42_51

81.0

666.6666666666666

Region

Note=O-glycosylated at one site

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

253_264

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

305_316

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

253_264

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

305_316

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

253_264

81.0

666.6666666666666

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

305_316

81.0

666.6666666666666

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

253_264

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

305_316

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

253_264

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

305_316

81.0

462.0

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

253_264

81.0

666.6666666666666

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

305_316

81.0

666.6666666666666

Calcium binding

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

150_218

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

341_407

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

150_218

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

341_407

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

150_218

81.0

666.6666666666666

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

341_407

81.0

666.6666666666666

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

150_218

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

341_407

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

150_218

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

341_407

81.0

462.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

150_218

81.0

666.6666666666666

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

341_407

81.0

666.6666666666666

Coiled coil

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

401_407

81.0

462.0

Compositional bias

Note=Poly-Gln

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

401_407

81.0

462.0

Compositional bias

Note=Poly-Gln

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

401_407

81.0

666.6666666666666

Compositional bias

Note=Poly-Gln

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

401_407

81.0

462.0

Compositional bias

Note=Poly-Gln

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

401_407

81.0

462.0

Compositional bias

Note=Poly-Gln

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

401_407

81.0

666.6666666666666

Compositional bias

Note=Poly-Gln

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

172_218

81.0

462.0

DNA binding

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

172_218

81.0

462.0

DNA binding

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

172_218

81.0

666.6666666666666

DNA binding

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

172_218

81.0

462.0

DNA binding

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

172_218

81.0

462.0

DNA binding

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

172_218

81.0

666.6666666666666

DNA binding

Ontology_term=ECO:0000255

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

240_275

81.0

462.0

Domain

EF-hand 1

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

292_327

81.0

462.0

Domain

EF-hand 2

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

240_275

81.0

462.0

Domain

EF-hand 1

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

292_327

81.0

462.0

Domain

EF-hand 2

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

240_275

81.0

666.6666666666666

Domain

EF-hand 1

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

292_327

81.0

666.6666666666666

Domain

EF-hand 2

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

240_275

81.0

462.0

Domain

EF-hand 1

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

292_327

81.0

462.0

Domain

EF-hand 2

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

240_275

81.0

462.0

Domain

EF-hand 1

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

292_327

81.0

462.0

Domain

EF-hand 2

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

240_275

81.0

666.6666666666666

Domain

EF-hand 1

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

292_327

81.0

666.6666666666666

Domain

EF-hand 2

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

303_333

81.0

462.0

Motif

GBA

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

303_333

81.0

462.0

Motif

GBA

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

303_333

81.0

666.6666666666666

Motif

GBA

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

303_333

81.0

462.0

Motif

GBA

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

303_333

81.0

462.0

Motif

GBA

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

303_333

81.0

666.6666666666666

Motif

GBA

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000263273

228_321

81.0

462.0

Region

Binds to GNAI2 and GNAI3

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000405315

228_321

81.0

462.0

Region

Binds to GNAI2 and GNAI3

Hgene

NUCB1

chr19:49407711

chr19:49513032

ENST00000407032

228_321

81.0

666.6666666666666

Region

Binds to GNAI2 and GNAI3

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000263273

228_321

81.0

462.0

Region

Binds to GNAI2 and GNAI3

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000405315

228_321

81.0

462.0

Region

Binds to GNAI2 and GNAI3

Hgene

NUCB1

chr19:49407711

chr19:49513033

ENST00000407032

228_321

81.0

666.6666666666666

Region

Binds to GNAI2 and GNAI3

Tgene

RUVBL2

chr19:49407711

chr19:49513032

ENST00000595090

77_84

154.0

464.0

Nucleotide binding

Note=ATP

Tgene

RUVBL2

chr19:49407711

chr19:49513033

ENST00000595090

77_84

154.0

464.0

Nucleotide binding

Note=ATP

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>781_NUCB1_49407711_RUVBL2_49513033_ranked_0.pdb	NUCB1	49407711	49407711	ENST00000601968	RUVBL2	chr19	49513033	+	MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKGSKVGKLTL KTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSR TQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSP HGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLF	390

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NUCB1_pLDDT.png

RUVBL2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NUCB1
RUVBL2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to NUCB1-RUVBL2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to NUCB1-RUVBL2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source