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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:OTUB1-RPS6KA4

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: OTUB1-RPS6KA4
	FusionPDB ID: 61954
	FusionGDB2.0 ID: 61954
		Hgene	Tgene
	Gene symbol	OTUB1	RPS6KA4
	Gene ID	55611	8986
	Gene name	OTU deubiquitinase, ubiquitin aldehyde binding 1	ribosomal protein S6 kinase A4
	Synonyms	HSPC263\|OTB1\|OTU1	MSK2\|RSK-B\|S6K-alpha-4
	Cytomap	11q13.1	11q13.1
	Type of gene	protein-coding	protein-coding
	Description	ubiquitin thioesterase OTUB1OTU domain, ubiquitin aldehyde binding 1OTU domain-containing ubiquitin aldehyde-binding protein 1OTU-domain Ubal-binding 1deubiquitinating enzyme OTUB1otubain-1ubiquitin-specific protease otubain 1ubiquitin-specific-pro	ribosomal protein S6 kinase alpha-490 kDa ribosomal protein S6 kinase 4mitogen- and stress-activated protein kinase 2nuclear mitogen- and stress-activated protein kinase 2ribosomal protein S6 kinase, 90kDa, polypeptide 4ribosomal protein kinase B
	Modification date	20200313	20200327
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000536443, ENST00000422031, ENST00000428192, ENST00000535715, ENST00000538426, ENST00000543004, ENST00000543988, ENST00000541478,	ENST00000294261, ENST00000334205, ENST00000528057,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	10 X 8 X 7=560	2 X 2 X 2=8
	# samples	10	3
	** MAII score	log2(10/560*10)=-2.48542682717024 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(3/8*10)=1.90689059560852
Context (manual curation of fusion genes in FusionPDB)	PubMed: OTUB1 [Title/Abstract] AND RPS6KA4 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	OTUB1(63756224)-RPS6KA4(64132773), # samples:2
Anticipated loss of major functional domain due to fusion event.	OTUB1-RPS6KA4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. OTUB1-RPS6KA4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. OTUB1-RPS6KA4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. OTUB1-RPS6KA4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	OTUB1	GO:0006974	cellular response to DNA damage stimulus	20725033
Hgene	OTUB1	GO:0071108	protein K48-linked deubiquitination	18954305\|19211026\|23827681
Hgene	OTUB1	GO:1901315	negative regulation of histone H2A K63-linked ubiquitination	20725033
Tgene	RPS6KA4	GO:0006355	regulation of transcription, DNA-templated	9792677
Tgene	RPS6KA4	GO:0006468	protein phosphorylation	9792677
Tgene	RPS6KA4	GO:0035556	intracellular signal transduction	9792677

Fusion gene breakpoints across OTUB1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across RPS6KA4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	HNSC	TCGA-CV-5973-01A	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+
ChimerDB4	HNSC	TCGA-CV-5973	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000535715	OTUB1	chr11	63756224	+	ENST00000528057	RPS6KA4	chr11	64132773	+	2798	671	410	2062	550
ENST00000535715	OTUB1	chr11	63756224	+	ENST00000334205	RPS6KA4	chr11	64132773	+	2819	671	410	2083	557
ENST00000535715	OTUB1	chr11	63756224	+	ENST00000294261	RPS6KA4	chr11	64132773	+	2074	671	410	1339	309
ENST00000422031	OTUB1	chr11	63756224	+	ENST00000528057	RPS6KA4	chr11	64132773	+	2669	542	11	1933	640
ENST00000422031	OTUB1	chr11	63756224	+	ENST00000334205	RPS6KA4	chr11	64132773	+	2690	542	11	1954	647
ENST00000422031	OTUB1	chr11	63756224	+	ENST00000294261	RPS6KA4	chr11	64132773	+	1945	542	11	1210	399
ENST00000428192	OTUB1	chr11	63756224	+	ENST00000528057	RPS6KA4	chr11	64132773	+	2669	542	11	1933	640
ENST00000428192	OTUB1	chr11	63756224	+	ENST00000334205	RPS6KA4	chr11	64132773	+	2690	542	11	1954	647
ENST00000428192	OTUB1	chr11	63756224	+	ENST00000294261	RPS6KA4	chr11	64132773	+	1945	542	11	1210	399
ENST00000538426	OTUB1	chr11	63756224	+	ENST00000528057	RPS6KA4	chr11	64132773	+	2390	263	2	1654	550
ENST00000538426	OTUB1	chr11	63756224	+	ENST00000334205	RPS6KA4	chr11	64132773	+	2411	263	2	1675	557
ENST00000538426	OTUB1	chr11	63756224	+	ENST00000294261	RPS6KA4	chr11	64132773	+	1666	263	2	931	309
ENST00000543004	OTUB1	chr11	63756224	+	ENST00000528057	RPS6KA4	chr11	64132773	+	2384	257	11	1648	545
ENST00000543004	OTUB1	chr11	63756224	+	ENST00000334205	RPS6KA4	chr11	64132773	+	2405	257	11	1669	552
ENST00000543004	OTUB1	chr11	63756224	+	ENST00000294261	RPS6KA4	chr11	64132773	+	1660	257	11	925	304
ENST00000543988	OTUB1	chr11	63756224	+	ENST00000528057	RPS6KA4	chr11	64132773	+	2485	358	160	1749	529
ENST00000543988	OTUB1	chr11	63756224	+	ENST00000334205	RPS6KA4	chr11	64132773	+	2506	358	160	1770	536
ENST00000543988	OTUB1	chr11	63756224	+	ENST00000294261	RPS6KA4	chr11	64132773	+	1761	358	160	1026	288

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000535715	ENST00000528057	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.017012663	0.9829874
ENST00000535715	ENST00000334205	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.016911488	0.98308855
ENST00000535715	ENST00000294261	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.019767744	0.98023224
ENST00000422031	ENST00000528057	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.040452994	0.95954704
ENST00000422031	ENST00000334205	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.03824892	0.96175104
ENST00000422031	ENST00000294261	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.041762106	0.9582379
ENST00000428192	ENST00000528057	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.040452994	0.95954704
ENST00000428192	ENST00000334205	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.03824892	0.96175104
ENST00000428192	ENST00000294261	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.041762106	0.9582379
ENST00000538426	ENST00000528057	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.015562777	0.9844372
ENST00000538426	ENST00000334205	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.015574767	0.9844252
ENST00000538426	ENST00000294261	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.020018062	0.9799819
ENST00000543004	ENST00000528057	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.013947786	0.98605216
ENST00000543004	ENST00000334205	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.015137224	0.98486274
ENST00000543004	ENST00000294261	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.008893638	0.99110633
ENST00000543988	ENST00000528057	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.014319226	0.9856808
ENST00000543988	ENST00000334205	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.015306815	0.98469317
ENST00000543988	ENST00000294261	OTUB1	chr11	63756224	+	RPS6KA4	chr11	64132773	+	0.03346605	0.96653396

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>61954_61954_1_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000422031_RPS6KA4_chr11_64132773_ENST00000294261_length(amino acids)=399AA_BP=1
MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAAEIMCKIREGRFSLDGEAWQGV
SEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQ

--------------------------------------------------------------

>61954_61954_2_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000422031_RPS6KA4_chr11_64132773_ENST00000334205_length(amino acids)=647AA_BP=1
MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLEL
LRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQY
AAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKL
EGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAP

--------------------------------------------------------------

>61954_61954_3_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000422031_RPS6KA4_chr11_64132773_ENST00000528057_length(amino acids)=640AA_BP=1
MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELL
EHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLA
QQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSS
WLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAP

--------------------------------------------------------------

>61954_61954_4_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000428192_RPS6KA4_chr11_64132773_ENST00000294261_length(amino acids)=399AA_BP=1
MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAAEIMCKIREGRFSLDGEAWQGV
SEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQ

--------------------------------------------------------------

>61954_61954_5_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000428192_RPS6KA4_chr11_64132773_ENST00000334205_length(amino acids)=647AA_BP=1
MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLEL
LRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQY
AAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKL
EGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAP

--------------------------------------------------------------

>61954_61954_6_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000428192_RPS6KA4_chr11_64132773_ENST00000528057_length(amino acids)=640AA_BP=1
MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELL
EHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLA
QQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSS
WLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAP

--------------------------------------------------------------

>61954_61954_7_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000535715_RPS6KA4_chr11_64132773_ENST00000294261_length(amino acids)=309AA_BP=1
MATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAAEIMCKIREGRFSLDGEAWQGV
SEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQ

--------------------------------------------------------------

>61954_61954_8_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000535715_RPS6KA4_chr11_64132773_ENST00000334205_length(amino acids)=557AA_BP=1
MATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLEL
LRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQY
AAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKL
EGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAP

--------------------------------------------------------------

>61954_61954_9_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000535715_RPS6KA4_chr11_64132773_ENST00000528057_length(amino acids)=550AA_BP=1
MATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELL
EHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLA
QQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSS
WLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAP

--------------------------------------------------------------

>61954_61954_10_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000538426_RPS6KA4_chr11_64132773_ENST00000294261_length(amino acids)=309AA_BP=0
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAAEIMCKIREGRFSLDGEAWQGV
SEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQ

--------------------------------------------------------------

>61954_61954_11_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000538426_RPS6KA4_chr11_64132773_ENST00000334205_length(amino acids)=557AA_BP=0
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLEL
LRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQY
AAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKL
EGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAP

--------------------------------------------------------------

>61954_61954_12_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000538426_RPS6KA4_chr11_64132773_ENST00000528057_length(amino acids)=550AA_BP=0
LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD
WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR
SAMMQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELL
EHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLA
QQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSS
WLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAP

--------------------------------------------------------------

>61954_61954_13_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000543004_RPS6KA4_chr11_64132773_ENST00000294261_length(amino acids)=304AA_BP=0
MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEDMTDPCLLQIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLDWVALA
ARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAAEIMCKIREGRFSLDGEAWQGVSEEAK
ELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSA

--------------------------------------------------------------

>61954_61954_14_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000543004_RPS6KA4_chr11_64132773_ENST00000334205_length(amino acids)=552AA_BP=0
MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEDMTDPCLLQIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLDWVALA
ARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQ
DSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGE
LLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPEL
LAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRG
SSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKG

--------------------------------------------------------------

>61954_61954_15_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000543004_RPS6KA4_chr11_64132773_ENST00000528057_length(amino acids)=545AA_BP=0
MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEDMTDPCLLQIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLDWVALA
ARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQ
YELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELLEHIRK
KRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLAQQGYD
ESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDG
SARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAPRRANG

--------------------------------------------------------------

>61954_61954_16_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000543988_RPS6KA4_chr11_64132773_ENST00000294261_length(amino acids)=288AA_BP=5
MRDLSLRPATFSGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLDWVALAARKIPAPFRPQIRSEL
DVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLK
LEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRA

--------------------------------------------------------------

>61954_61954_17_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000543988_RPS6KA4_chr11_64132773_ENST00000334205_length(amino acids)=536AA_BP=5
MRDLSLRPATFSGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLDWVALAARKIPAPFRPQIRSEL
DVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQDSPFFQQYELDLREPA
LGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELLEHIRKKRHFSESEA
SQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLAQQGYDESCDLWSLG
VILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLR

--------------------------------------------------------------

>61954_61954_18_OTUB1-RPS6KA4_OTUB1_chr11_63756224_ENST00000543988_RPS6KA4_chr11_64132773_ENST00000528057_length(amino acids)=529AA_BP=5
MRDLSLRPATFSGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLDWVALAARKIPAPFRPQIRSEL
DVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQYELDLREPALGQGSFS
VCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELLEHIRKKRHFSESEASQILRSL
VSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMML
SGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLES

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:63756224/chr11:64132773)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

RPS6KA4

chr11:63756224

chr11:64132773

ENST00000334205

302_371

302.0

773.0

Domain

AGC-kinase C-terminal

Tgene

RPS6KA4

chr11:63756224

chr11:64132773

ENST00000334205

411_674

302.0

773.0

Domain

Protein kinase 2

Tgene

RPS6KA4

chr11:63756224

chr11:64132773

ENST00000334205

417_425

302.0

773.0

Nucleotide binding

ATP

Tgene

RPS6KA4

chr11:63756224

chr11:64132773

ENST00000334205

725_772

302.0

773.0

Region

Note=Required for nuclear targeting and association with MAPK14

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

80_271

73.0

272.0

Domain

OTU

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

80_271

73.0

272.0

Domain

OTU

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

130_138

73.0

272.0

Region

Ubiquitin-conjugating enzyme E2 binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

169_177

73.0

272.0

Region

Ubiquitin-conjugating enzyme E2 binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

189_195

73.0

272.0

Region

Free ubiquitin binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

206_213

73.0

272.0

Region

Ubiquitin-conjugating enzyme E2 binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

214_221

73.0

272.0

Region

Free ubiquitin binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000428192

245_251

73.0

272.0

Region

Free ubiquitin binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

130_138

73.0

272.0

Region

Ubiquitin-conjugating enzyme E2 binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

169_177

73.0

272.0

Region

Ubiquitin-conjugating enzyme E2 binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

189_195

73.0

272.0

Region

Free ubiquitin binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

206_213

73.0

272.0

Region

Ubiquitin-conjugating enzyme E2 binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

214_221

73.0

272.0

Region

Free ubiquitin binding

Hgene

OTUB1

chr11:63756224

chr11:64132773

ENST00000538426

245_251

73.0

272.0

Region

Free ubiquitin binding

Tgene

RPS6KA4

chr11:63756224

chr11:64132773

ENST00000334205

33_301

302.0

773.0

Domain

Protein kinase 1

Tgene

RPS6KA4

chr11:63756224

chr11:64132773

ENST00000334205

39_47

302.0

773.0

Nucleotide binding

ATP

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1352_OTUB1_63756224_RPS6KA4_64132773_1352_OTUB1_63756224_RPS6KA4_64132773_ranked_0.pdb	OTUB1	63756224	63756224	ENST00000294261	RPS6KA4	chr11	64132773	+	MQLWSHAVPAEARSRNAAFGDFNFTFLLLSITKSAFQTPGKRKRNWLQEDLQSPPGTLGGWEDGAKPMCGVEGCCQGGATRRGSLPGGRQ LATRKRSVVRRCLKMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKGLD WVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVAR SAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLEL LRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQY AAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKL EGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAP	647

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

OTUB1_pLDDT.png

RPS6KA4_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
OTUB1
RPS6KA4

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to OTUB1-RPS6KA4

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to OTUB1-RPS6KA4

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source