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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ARHGEF18-MAPKAP1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ARHGEF18-MAPKAP1
	FusionPDB ID: 6240
	FusionGDB2.0 ID: 6240
		Hgene	Tgene
	Gene symbol	ARHGEF18	MAPKAP1
	Gene ID	23370	79109
	Gene name	Rho/Rac guanine nucleotide exchange factor 18	MAPK associated protein 1
	Synonyms	P114-RhoGEF\|RP78\|SA-RhoGEF\|p114RhoGEF	JC310\|MIP1\|SIN1\|SIN1b\|SIN1g
	Cytomap	19p13.2	9q33.3
	Type of gene	protein-coding	protein-coding
	Description	rho guanine nucleotide exchange factor 18114 kDa Rho-specific guanine nucleotide exchange factorRho-specific guanine nucleotide exchange factor p114Rho/Rac guanine nucleotide exchange factor (GEF) 18septin-associated RhoGEF	target of rapamycin complex 2 subunit MAPKAP1MEKK2-interacting protein 1SAPK-interacting protein 1TORC2 subunit MAPKAP1mSIN1mitogen-activated protein kinase 2-associated protein 1mitogen-activated protein kinase associated protein 1ras inhibitor MG
	Modification date	20200320	20200329
	UniProtAcc	Q6ZSZ5	Q9BPZ7
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000319670, ENST00000359920,	ENST00000483937, ENST00000265960, ENST00000350766, ENST00000373497, ENST00000373498, ENST00000373503, ENST00000373511, ENST00000394060, ENST00000394063,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	14 X 8 X 12=1344	12 X 10 X 10=1200
	# samples	16	13
	** MAII score	log2(16/1344*10)=-3.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(13/1200*10)=-3.20645087746743 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ARHGEF18 [Title/Abstract] AND MAPKAP1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ARHGEF18(7506938)-MAPKAP1(128230388), # samples:3
Anticipated loss of major functional domain due to fusion event.	ARHGEF18-MAPKAP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ARHGEF18-MAPKAP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ARHGEF18-MAPKAP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ARHGEF18-MAPKAP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ARHGEF18	GO:0007264	small GTPase mediated signal transduction	14512443
Hgene	ARHGEF18	GO:0008360	regulation of cell shape	14512443
Hgene	ARHGEF18	GO:0030036	actin cytoskeleton organization	14512443

Fusion gene breakpoints across ARHGEF18 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across MAPKAP1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	COAD	TCGA-AD-6901-01A	ARHGEF18	chr19	7506938	-	MAPKAP1	chr9	128230388	-
ChimerDB4	COAD	TCGA-AD-6901-01A	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-
ChimerDB4	COAD	TCGA-AD-6901-01A	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128233694	-
ChimerDB4	COAD	TCGA-AD-6901	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000373511	MAPKAP1	chr9	128230388	-	2384	528	80	889	269
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000373498	MAPKAP1	chr9	128230388	-	2383	528	80	889	269
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000350766	MAPKAP1	chr9	128230388	-	2383	528	80	889	269
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000373503	MAPKAP1	chr9	128230388	-	2383	528	80	889	269
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000394063	MAPKAP1	chr9	128230388	-	2383	528	80	889	269
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000265960	MAPKAP1	chr9	128230388	-	2383	528	80	889	269
ENST00000319670	ARHGEF18	chr19	7506938	+	ENST00000373497	MAPKAP1	chr9	128230388	-	1244	528	80	889	269
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000373511	MAPKAP1	chr9	128230388	-	2905	1049	253	1410	385
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000373498	MAPKAP1	chr9	128230388	-	2904	1049	253	1410	385
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000350766	MAPKAP1	chr9	128230388	-	2904	1049	253	1410	385
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000373503	MAPKAP1	chr9	128230388	-	2904	1049	253	1410	385
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000394063	MAPKAP1	chr9	128230388	-	2904	1049	253	1410	385
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000265960	MAPKAP1	chr9	128230388	-	2904	1049	253	1410	385
ENST00000359920	ARHGEF18	chr19	7506938	+	ENST00000373497	MAPKAP1	chr9	128230388	-	1765	1049	253	1410	385

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000319670	ENST00000373511	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.003759675	0.9962404
ENST00000319670	ENST00000373498	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.003738596	0.9962614
ENST00000319670	ENST00000350766	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.003738596	0.9962614
ENST00000319670	ENST00000373503	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.003738596	0.9962614
ENST00000319670	ENST00000394063	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.003738596	0.9962614
ENST00000319670	ENST00000265960	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.003738596	0.9962614
ENST00000319670	ENST00000373497	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.007143709	0.99285626
ENST00000359920	ENST00000373511	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.001915971	0.99808407
ENST00000359920	ENST00000373498	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.001925572	0.9980744
ENST00000359920	ENST00000350766	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.001925572	0.9980744
ENST00000359920	ENST00000373503	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.001925572	0.9980744
ENST00000359920	ENST00000394063	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.001925572	0.9980744
ENST00000359920	ENST00000265960	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.001925572	0.9980744
ENST00000359920	ENST00000373497	ARHGEF18	chr19	7506938	+	MAPKAP1	chr9	128230388	-	0.009232808	0.9907672

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>6240_6240_1_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000265960_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_2_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000350766_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_3_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000373497_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_4_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000373498_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_5_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000373503_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_6_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000373511_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_7_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000319670_MAPKAP1_chr9_128230388_ENST00000394063_length(amino acids)=269AA_BP=147
MREWQRSWRRAACGRSGRASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLS
LTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGDKVEIDPVTNQKASTKFWIKQKPISID

--------------------------------------------------------------

>6240_6240_8_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000265960_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

>6240_6240_9_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000350766_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

>6240_6240_10_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000373497_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

>6240_6240_11_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000373498_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

>6240_6240_12_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000373503_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

>6240_6240_13_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000373511_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

>6240_6240_14_ARHGEF18-MAPKAP1_ARHGEF18_chr19_7506938_ENST00000359920_MAPKAP1_chr9_128230388_ENST00000394063_length(amino acids)=385AA_BP=263
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF
LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL
GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD
KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:7506938/chr9:128230388)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ARHGEF18 Q6ZSZ5	MAPKAP1 Q9BPZ7
FUNCTION: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). {ECO:0000269\|PubMed:11085924, ECO:0000269\|PubMed:14512443, ECO:0000269\|PubMed:15558029, ECO:0000269\|PubMed:22006950, ECO:0000269\|PubMed:28132693}.	FUNCTION: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex. {ECO:0000269\|PubMed:15988011, ECO:0000269\|PubMed:16962653, ECO:0000269\|PubMed:17043309, ECO:0000269\|PubMed:17054722, ECO:0000269\|PubMed:17303383, ECO:0000269\|PubMed:23727834}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000319670

1038_1148

107.33333333333333

1618.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000359920

1038_1148

265.3333333333333

1556.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000319670

1042_1142

107.33333333333333

1618.0

Compositional bias

Arg-rich

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000319670

1292_1346

107.33333333333333

1618.0

Compositional bias

Pro-rich

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000359920

1042_1142

265.3333333333333

1556.0

Compositional bias

Arg-rich

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000359920

1292_1346

265.3333333333333

1556.0

Compositional bias

Pro-rich

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000319670

447_644

107.33333333333333

1618.0

Domain

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000319670

684_786

107.33333333333333

1618.0

Domain

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000359920

447_644

265.3333333333333

1556.0

Domain

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000359920

684_786

265.3333333333333

1556.0

Domain

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000319670

310_334

107.33333333333333

1618.0

Zinc finger

C2H2-type%3B degenerate

Hgene

ARHGEF18

chr19:7506938

chr9:128230388

ENST00000359920

310_334

265.3333333333333

1556.0

Zinc finger

C2H2-type%3B degenerate

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>767_ARHGEF18_7506938_MAPKAP1_128230388_ranked_0.pdb	ARHGEF18	7506938	7506938	ENST00000373497	MAPKAP1	chr9	128230388	-	MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVF LDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYGISGD KVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADY	385

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ARHGEF18_pLDDT.png

MAPKAP1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ARHGEF18
MAPKAP1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000265960		8	12	2_184	402.3333333333333	523.0	MAP3K2
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000350766		7	11	2_184	366.3333333333333	487.0	MAP3K2
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000373498		7	11	2_184	402.3333333333333	523.0	MAP3K2
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000373503		7	11	2_184	210.33333333333334	331.0	MAP3K2
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000373511		7	11	2_184	355.3333333333333	476.0	MAP3K2
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000394063		6	10	2_184	210.33333333333334	331.0	MAP3K2
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000265960		8	12	2_267	402.3333333333333	523.0	NBN
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000350766		7	11	2_267	366.3333333333333	487.0	NBN
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000373498		7	11	2_267	402.3333333333333	523.0	NBN
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000373503		7	11	2_267	210.33333333333334	331.0	NBN
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000373511		7	11	2_267	355.3333333333333	476.0	NBN
Tgene	MAPKAP1	chr19:7506938	chr9:128230388	ENST00000394063		6	10	2_267	210.33333333333334	331.0	NBN

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Related Drugs to ARHGEF18-MAPKAP1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to ARHGEF18-MAPKAP1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source