UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine level1
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:PCCA-GRM3

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: PCCA-GRM3
FusionPDB ID: 63154
FusionGDB2.0 ID: 63154
HgeneTgene
Gene symbol

PCCA

GRM3

Gene ID

5095

2913

Gene namepropionyl-CoA carboxylase subunit alphaglutamate metabotropic receptor 3
Synonyms-GLUR3|GPRC1C|MGLUR3|mGlu3
Cytomap

13q32.3

7q21.11-q21.12

Type of geneprotein-codingprotein-coding
Descriptionpropionyl-CoA carboxylase alpha chain, mitochondrialPCCase alpha subunitpccA complementation grouppropanoyl-CoA:carbon dioxide ligase alpha subunitpropionyl CoA carboxylase, alpha polypeptidepropionyl Coenzyme A carboxylase, alpha polypeptidepropionmetabotropic glutamate receptor 3glutamate receptor, metabotropic 3
Modification date2020031320200313
UniProtAcc.

Q14832

Ensembl transtripts involved in fusion geneENST idsENST00000376285, ENST00000376286, 
ENST00000376279, ENST00000485946, 
ENST00000394720, ENST00000439827, 
ENST00000536043, ENST00000546348, 
ENST00000361669, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score20 X 16 X 12=38406 X 6 X 3=108
# samples 256
** MAII scorelog2(25/3840*10)=-3.94110631094643
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/108*10)=-0.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: PCCA [Title/Abstract] AND GRM3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)PCCA(101167821)-GRM3(86415576), # samples:1
Anticipated loss of major functional domain due to fusion event.PCCA-GRM3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
PCCA-GRM3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
PCCA-GRM3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
PCCA-GRM3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across PCCA (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across GRM3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SARCTCGA-SI-A71OPCCAchr13

101167821

+GRM3chr7

86415576

+


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000376286PCCAchr13101167821+ENST00000361669GRM3chr786415576+476920686442391391
ENST00000376285PCCAchr13101167821+ENST00000361669GRM3chr786415576+477920783842491403

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000376286ENST00000361669PCCAchr13101167821+GRM3chr786415576+0.0004606480.9995394
ENST00000376285ENST00000361669PCCAchr13101167821+GRM3chr786415576+0.0003511250.9996489

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>63154_63154_1_PCCA-GRM3_PCCA_chr13_101167821_ENST00000376285_GRM3_chr7_86415576_ENST00000361669_length(amino acids)=1403AA_BP=680
MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTV
AIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIES
KLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHI
EIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPV
TECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMIS
KLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRA
QHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFL
GTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFY
QAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFEQEARLRNICIATAEKVGRSNIRKSYDSVIRELLQKPNARVVVLFMRSDDSRELIA
AASRANASFTWVASDGWGAQESIIKGSEHVAYGAITLELASQPVRQFDRYFQSLNPYNNHRNPWFRDFWEQKFQCSLQNKRNHRRVCDKH
LAIDSSNYEQESKIMFVVNAVYAMAHALHKMQRTLCPNTTKLCDAMKILDGKKLYKDYLLKINFTAPFNPNKDADSIVKFDTFGDGMGRY
NVFNFQNVGGKYSYLKVGHWAETLSLDVNSIHWSRNSVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEPYEYLADEFTCMDCGSGQWPT
ADLTGCYDLPEDYIRWEDAWAIGPVTIACLGFMCTCMVVTVFIKHNNTPLVKASGRELCYILLFGVGLSYCMTFFFIAKPSPVICALRRL
GLGSSFAICYSALLTKTNCIARIFDGVKNGAQRPKFISPSSQVFICLGLILVQIVMVSVWLILEAPGTRRYTLAEKRETVILKCNVKDSS
MLISLTYDVILVILCTVYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHII

--------------------------------------------------------------

>63154_63154_2_PCCA-GRM3_PCCA_chr13_101167821_ENST00000376286_GRM3_chr7_86415576_ENST00000361669_length(amino acids)=1391AA_BP=668
MCERSAEGRSAGTTMAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVH
VKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTI
PGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNA
LWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQE
MIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEA
LKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVI
KPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRL
AAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFF
NWTYVSTVASEGDYGETGIEAFEQEARLRNICIATAEKVGRSNIRKSYDSVIRELLQKPNARVVVLFMRSDDSRELIAAASRANASFTWV
ASDGWGAQESIIKGSEHVAYGAITLELASQPVRQFDRYFQSLNPYNNHRNPWFRDFWEQKFQCSLQNKRNHRRVCDKHLAIDSSNYEQES
KIMFVVNAVYAMAHALHKMQRTLCPNTTKLCDAMKILDGKKLYKDYLLKINFTAPFNPNKDADSIVKFDTFGDGMGRYNVFNFQNVGGKY
SYLKVGHWAETLSLDVNSIHWSRNSVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEPYEYLADEFTCMDCGSGQWPTADLTGCYDLPED
YIRWEDAWAIGPVTIACLGFMCTCMVVTVFIKHNNTPLVKASGRELCYILLFGVGLSYCMTFFFIAKPSPVICALRRLGLGSSFAICYSA
LLTKTNCIARIFDGVKNGAQRPKFISPSSQVFICLGLILVQIVMVSVWLILEAPGTRRYTLAEKRETVILKCNVKDSSMLISLTYDVILV
ILCTVYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKNVVTHR

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr13:101167821/chr7:86415576)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.GRM3

Q14832

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity. {ECO:0000269|PubMed:8840013}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePCCAchr13:101167821chr7:86415576ENST00000376285+2224181_378680.0729.0DomainATP-grasp
HgenePCCAchr13:101167821chr7:86415576ENST00000376285+222462_509680.0729.0DomainBiotin carboxylation
HgenePCCAchr13:101167821chr7:86415576ENST00000376286+2123181_378654.0703.0DomainATP-grasp
HgenePCCAchr13:101167821chr7:86415576ENST00000376286+212362_509654.0703.0DomainBiotin carboxylation
HgenePCCAchr13:101167821chr7:86415576ENST00000376285+2224209_270680.0729.0Nucleotide bindingATP
HgenePCCAchr13:101167821chr7:86415576ENST00000376286+2123209_270654.0703.0Nucleotide bindingATP
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916172_174156.0880.0RegionGlutamate binding
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015172_174154.0536.0RegionGlutamate binding
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715172_174156.0538.0RegionGlutamate binding
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916600_613156.0880.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916635_645156.0880.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916665_688156.0880.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916710_734156.0880.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916757_769156.0880.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916793_802156.0880.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916829_879156.0880.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015600_613154.0536.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015635_645154.0536.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015665_688154.0536.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015710_734154.0536.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015757_769154.0536.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015793_802154.0536.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015829_879154.0536.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715600_613156.0538.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715635_645156.0538.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715665_688156.0538.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715710_734156.0538.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715757_769156.0538.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715793_802156.0538.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715829_879156.0538.0Topological domainCytoplasmic
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916577_599156.0880.0TransmembraneHelical%3B Name%3D1
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916614_634156.0880.0TransmembraneHelical%3B Name%3D2
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916646_664156.0880.0TransmembraneHelical%3B Name%3D3
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916689_709156.0880.0TransmembraneHelical%3B Name%3D4
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916735_756156.0880.0TransmembraneHelical%3B Name%3D5
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916770_792156.0880.0TransmembraneHelical%3B Name%3D6
TgeneGRM3chr13:101167821chr7:86415576ENST0000036166916803_828156.0880.0TransmembraneHelical%3B Name%3D7
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015577_599154.0536.0TransmembraneHelical%3B Name%3D1
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015614_634154.0536.0TransmembraneHelical%3B Name%3D2
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015646_664154.0536.0TransmembraneHelical%3B Name%3D3
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015689_709154.0536.0TransmembraneHelical%3B Name%3D4
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015735_756154.0536.0TransmembraneHelical%3B Name%3D5
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015770_792154.0536.0TransmembraneHelical%3B Name%3D6
TgeneGRM3chr13:101167821chr7:86415576ENST0000039472015803_828154.0536.0TransmembraneHelical%3B Name%3D7
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715577_599156.0538.0TransmembraneHelical%3B Name%3D1
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715614_634156.0538.0TransmembraneHelical%3B Name%3D2
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715646_664156.0538.0TransmembraneHelical%3B Name%3D3
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715689_709156.0538.0TransmembraneHelical%3B Name%3D4
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715735_756156.0538.0TransmembraneHelical%3B Name%3D5
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715770_792156.0538.0TransmembraneHelical%3B Name%3D6
TgeneGRM3chr13:101167821chr7:86415576ENST0000043982715803_828156.0538.0TransmembraneHelical%3B Name%3D7

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePCCAchr13:101167821chr7:86415576ENST00000376279+123181_3780682.0DomainATP-grasp
HgenePCCAchr13:101167821chr7:86415576ENST00000376279+12362_5090682.0DomainBiotin carboxylation
HgenePCCAchr13:101167821chr7:86415576ENST00000376279+123653_7280682.0DomainBiotinyl-binding
HgenePCCAchr13:101167821chr7:86415576ENST00000376285+2224653_728680.0729.0DomainBiotinyl-binding
HgenePCCAchr13:101167821chr7:86415576ENST00000376286+2123653_728654.0703.0DomainBiotinyl-binding
HgenePCCAchr13:101167821chr7:86415576ENST00000376279+123209_2700682.0Nucleotide bindingATP
TgeneGRM3chr13:101167821chr7:86415576ENST000003616691623_576156.0880.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST000003947201523_576154.0536.0Topological domainExtracellular
TgeneGRM3chr13:101167821chr7:86415576ENST000004398271523_576156.0538.0Topological domainExtracellular


Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
PCCA
GRM3


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs to PCCA-GRM3


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to PCCA-GRM3


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource