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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:POFUT1-HCK

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: POFUT1-HCK
	FusionPDB ID: 66835
	FusionGDB2.0 ID: 66835
		Hgene	Tgene
	Gene symbol	POFUT1	HCK
	Gene ID	23509	3055
	Gene name	protein O-fucosyltransferase 1	HCK proto-oncogene, Src family tyrosine kinase
	Synonyms	DDD2\|FUT12\|O-FUT\|O-Fuc-T\|O-FucT-1\|OFUCT1	JTK9\|p59Hck\|p61Hck
	Cytomap	20q11.21	20q11.21
	Type of gene	protein-coding	protein-coding
	Description	GDP-fucose protein O-fucosyltransferase 1o-fucosyltransferase proteinpeptide-O-fucosyltransferase 1	tyrosine-protein kinase HCKhematopoietic cell kinasehemopoietic cell kinasep59-HCK/p60-HCK
	Modification date	20200313	20200329
	UniProtAcc	.	P08631
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000486717, ENST00000539210, ENST00000375749, ENST00000375730,	ENST00000375852, ENST00000375862, ENST00000518730, ENST00000520553, ENST00000534862, ENST00000538448,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	7 X 5 X 7=245	10 X 10 X 7=700
	# samples	9	14
	** MAII score	log2(9/245*10)=-1.4447848426729 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(14/700*10)=-2.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: POFUT1 [Title/Abstract] AND HCK [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	POFUT1(30818864)-HCK(30689120), # samples:3
Anticipated loss of major functional domain due to fusion event.	POFUT1-HCK seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. POFUT1-HCK seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. POFUT1-HCK seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. POFUT1-HCK seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. POFUT1-HCK seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. POFUT1-HCK seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	POFUT1	GO:0006493	protein O-linked glycosylation	9023546\|11524432
Hgene	POFUT1	GO:0036066	protein O-linked fucosylation	15653671
Tgene	HCK	GO:0071801	regulation of podosome assembly	15998323
Tgene	HCK	GO:2000251	positive regulation of actin cytoskeleton reorganization	15998323

Fusion gene breakpoints across POFUT1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across HCK (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LIHC	TCGA-G3-A25X-01A	POFUT1	chr20	30818864	-	HCK	chr20	30689120	+
ChimerDB4	LIHC	TCGA-G3-A25X-01A	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+
ChimerDB4	OV	TCGA-04-1517-01A	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000375749	POFUT1	chr20	30818864	+	ENST00000534862	HCK	chr20	30689120	+	1576	1040	26	1060	344
ENST00000375749	POFUT1	chr20	30818864	+	ENST00000538448	HCK	chr20	30689120	+	1576	1040	26	1060	344
ENST00000375749	POFUT1	chr20	30818864	+	ENST00000375862	HCK	chr20	30689120	+	1243	1040	26	1060	344
ENST00000375749	POFUT1	chr20	30818864	+	ENST00000518730	HCK	chr20	30689120	+	1243	1040	26	1060	344
ENST00000539210	POFUT1	chr20	30818864	+	ENST00000534862	HCK	chr20	30689120	+	1145	609	132	629	165
ENST00000539210	POFUT1	chr20	30818864	+	ENST00000538448	HCK	chr20	30689120	+	1145	609	132	629	165
ENST00000539210	POFUT1	chr20	30818864	+	ENST00000375862	HCK	chr20	30689120	+	812	609	132	629	165
ENST00000539210	POFUT1	chr20	30818864	+	ENST00000518730	HCK	chr20	30689120	+	812	609	132	629	165
ENST00000375749	POFUT1	chr20	30795868	+	ENST00000534862	HCK	chr20	30671697	+	1568	186	26	1234	402
ENST00000375749	POFUT1	chr20	30795868	+	ENST00000538448	HCK	chr20	30671697	+	1568	186	26	1234	402
ENST00000375749	POFUT1	chr20	30795868	+	ENST00000375862	HCK	chr20	30671697	+	1235	186	26	1234	403
ENST00000375749	POFUT1	chr20	30795868	+	ENST00000520553	HCK	chr20	30671697	+	1572	186	26	1234	402
ENST00000375749	POFUT1	chr20	30795868	+	ENST00000518730	HCK	chr20	30671697	+	1235	186	26	1234	403
ENST00000375749	POFUT1	chr20	30795868	+	ENST00000375852	HCK	chr20	30671697	+	1572	186	26	1234	402
ENST00000375730	POFUT1	chr20	30795868	+	ENST00000534862	HCK	chr20	30671697	+	1555	173	13	1221	402
ENST00000375730	POFUT1	chr20	30795868	+	ENST00000538448	HCK	chr20	30671697	+	1555	173	13	1221	402
ENST00000375730	POFUT1	chr20	30795868	+	ENST00000375862	HCK	chr20	30671697	+	1222	173	13	1221	403
ENST00000375730	POFUT1	chr20	30795868	+	ENST00000520553	HCK	chr20	30671697	+	1559	173	13	1221	402
ENST00000375730	POFUT1	chr20	30795868	+	ENST00000518730	HCK	chr20	30671697	+	1222	173	13	1221	403
ENST00000375730	POFUT1	chr20	30795868	+	ENST00000375852	HCK	chr20	30671697	+	1559	173	13	1221	402

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000375749	ENST00000534862	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.08922881	0.9107712
ENST00000375749	ENST00000538448	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.08922881	0.9107712
ENST00000375749	ENST00000375862	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.12722719	0.8727728
ENST00000375749	ENST00000518730	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.12722719	0.8727728
ENST00000539210	ENST00000534862	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.07041362	0.9295864
ENST00000539210	ENST00000538448	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.07041362	0.9295864
ENST00000539210	ENST00000375862	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.05317785	0.9468222
ENST00000539210	ENST00000518730	POFUT1	chr20	30818864	+	HCK	chr20	30689120	+	0.05317785	0.9468222
ENST00000375749	ENST00000534862	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.008371824	0.99162817
ENST00000375749	ENST00000538448	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.008371824	0.99162817
ENST00000375749	ENST00000375862	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.009377508	0.99062246
ENST00000375749	ENST00000520553	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.008173939	0.99182606
ENST00000375749	ENST00000518730	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.009377508	0.99062246
ENST00000375749	ENST00000375852	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.008173939	0.99182606
ENST00000375730	ENST00000534862	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.007787737	0.9922123
ENST00000375730	ENST00000538448	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.007787737	0.9922123
ENST00000375730	ENST00000375862	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.008713197	0.9912868
ENST00000375730	ENST00000520553	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.007601149	0.9923989
ENST00000375730	ENST00000518730	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.008713197	0.9912868
ENST00000375730	ENST00000375852	POFUT1	chr20	30795868	+	HCK	chr20	30671697	+	0.007601149	0.9923989

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>66835_66835_1_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375730_HCK_chr20_30671697_ENST00000375852_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

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>66835_66835_2_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375730_HCK_chr20_30671697_ENST00000375862_length(amino acids)=403AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_3_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375730_HCK_chr20_30671697_ENST00000518730_length(amino acids)=403AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_4_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375730_HCK_chr20_30671697_ENST00000520553_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

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>66835_66835_5_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375730_HCK_chr20_30671697_ENST00000534862_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

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>66835_66835_6_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375730_HCK_chr20_30671697_ENST00000538448_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_7_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375749_HCK_chr20_30671697_ENST00000375852_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_8_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375749_HCK_chr20_30671697_ENST00000375862_length(amino acids)=403AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_9_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375749_HCK_chr20_30671697_ENST00000518730_length(amino acids)=403AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

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>66835_66835_10_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375749_HCK_chr20_30671697_ENST00000520553_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_11_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375749_HCK_chr20_30671697_ENST00000534862_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_12_POFUT1-HCK_POFUT1_chr20_30795868_ENST00000375749_HCK_chr20_30671697_ENST00000538448_length(amino acids)=402AA_BP=53
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRS
TFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLA
EANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLV
CKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPE

--------------------------------------------------------------

>66835_66835_13_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000375749_HCK_chr20_30689120_ENST00000375862_length(amino acids)=344AA_BP=
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKP
PFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGI
SFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAG

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>66835_66835_14_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000375749_HCK_chr20_30689120_ENST00000518730_length(amino acids)=344AA_BP=
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKP
PFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGI
SFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAG

--------------------------------------------------------------

>66835_66835_15_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000375749_HCK_chr20_30689120_ENST00000534862_length(amino acids)=344AA_BP=
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKP
PFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGI
SFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAG

--------------------------------------------------------------

>66835_66835_16_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000375749_HCK_chr20_30689120_ENST00000538448_length(amino acids)=344AA_BP=
MCAAAGSGSRADMGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKP
PFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGI
SFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAG

--------------------------------------------------------------

>66835_66835_17_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000539210_HCK_chr20_30689120_ENST00000375862_length(amino acids)=165AA_BP=
MGPGRLPALLPLHGFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTA

--------------------------------------------------------------

>66835_66835_18_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000539210_HCK_chr20_30689120_ENST00000518730_length(amino acids)=165AA_BP=
MGPGRLPALLPLHGFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTA

--------------------------------------------------------------

>66835_66835_19_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000539210_HCK_chr20_30689120_ENST00000534862_length(amino acids)=165AA_BP=
MGPGRLPALLPLHGFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTA

--------------------------------------------------------------

>66835_66835_20_POFUT1-HCK_POFUT1_chr20_30818864_ENST00000539210_HCK_chr20_30689120_ENST00000538448_length(amino acids)=165AA_BP=
MGPGRLPALLPLHGFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTA

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr20:30818864/chr20:30689120)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	HCK P08631
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS. {ECO:0000269\|PubMed:10092522, ECO:0000269\|PubMed:10779760, ECO:0000269\|PubMed:10973280, ECO:0000269\|PubMed:11741929, ECO:0000269\|PubMed:11896602, ECO:0000269\|PubMed:12411494, ECO:0000269\|PubMed:15010462, ECO:0000269\|PubMed:15952790, ECO:0000269\|PubMed:15998323, ECO:0000269\|PubMed:17310994, ECO:0000269\|PubMed:17535448, ECO:0000269\|PubMed:19114024, ECO:0000269\|PubMed:19903482, ECO:0000269\|PubMed:20452982, ECO:0000269\|PubMed:21338576, ECO:0000269\|PubMed:7535819, ECO:0000269\|PubMed:8132624, ECO:0000269\|PubMed:9406996, ECO:0000269\|PubMed:9407116}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375749

238_240

326.0

389.0

Region

Substrate binding

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375749

43_46

326.0

389.0

Region

Substrate binding

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000518730

262_515

155.33333333333334

505.0

Domain

Protein kinase

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000520553

262_515

156.33333333333334

506.0

Domain

Protein kinase

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000538448

262_515

156.33333333333334

506.0

Domain

Protein kinase

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000518730

268_276

155.33333333333334

505.0

Nucleotide binding

Note=ATP

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000520553

268_276

156.33333333333334

506.0

Nucleotide binding

Note=ATP

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000538448

268_276

156.33333333333334

506.0

Nucleotide binding

Note=ATP

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375730

385_388

41.333333333333336

195.0

Motif

Prevents secretion from ER

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375749

385_388

41.333333333333336

389.0

Motif

Prevents secretion from ER

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375730

385_388

195.0

Motif

Prevents secretion from ER

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375749

385_388

326.0

389.0

Motif

Prevents secretion from ER

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375730

238_240

41.333333333333336

195.0

Region

Substrate binding

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375730

357_358

41.333333333333336

195.0

Region

Substrate binding

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375730

43_46

41.333333333333336

195.0

Region

Substrate binding

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375749

238_240

41.333333333333336

389.0

Region

Substrate binding

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375749

357_358

41.333333333333336

389.0

Region

Substrate binding

Hgene

POFUT1

chr20:30795868

chr20:30671697

ENST00000375749

43_46

41.333333333333336

389.0

Region

Substrate binding

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375730

238_240

195.0

Region

Substrate binding

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375730

357_358

195.0

Region

Substrate binding

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375730

43_46

195.0

Region

Substrate binding

Hgene

POFUT1

chr20:30818864

chr20:30689120

ENST00000375749

357_358

326.0

389.0

Region

Substrate binding

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000518730

144_241

155.33333333333334

505.0

Domain

SH2

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000518730

78_138

155.33333333333334

505.0

Domain

SH3

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000520553

144_241

156.33333333333334

506.0

Domain

SH2

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000520553

78_138

156.33333333333334

506.0

Domain

SH3

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000538448

144_241

156.33333333333334

506.0

Domain

SH2

Tgene

HCK

chr20:30795868

chr20:30671697

ENST00000538448

78_138

156.33333333333334

506.0

Domain

SH3

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000518730

144_241

437.3333333333333

505.0

Domain

SH2

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000518730

262_515

437.3333333333333

505.0

Domain

Protein kinase

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000518730

78_138

437.3333333333333

505.0

Domain

SH3

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000520553

144_241

438.3333333333333

506.0

Domain

SH2

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000520553

262_515

438.3333333333333

506.0

Domain

Protein kinase

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000520553

78_138

438.3333333333333

506.0

Domain

SH3

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000538448

144_241

438.3333333333333

506.0

Domain

SH2

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000538448

262_515

438.3333333333333

506.0

Domain

Protein kinase

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000538448

78_138

438.3333333333333

506.0

Domain

SH3

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000518730

268_276

437.3333333333333

505.0

Nucleotide binding

Note=ATP

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000520553

268_276

438.3333333333333

506.0

Nucleotide binding

Note=ATP

Tgene

HCK

chr20:30818864

chr20:30689120

ENST00000538448

268_276

438.3333333333333

506.0

Nucleotide binding

Note=ATP

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
POFUT1
HCK

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to POFUT1-HCK

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to POFUT1-HCK

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source