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Center for Computational Systems Medicine level3
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Potential Active Site Information

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Potentially Interacting Small Molecules through Virtual Screening

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Biochemical Features of Small Molecules with ADME

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Drug Toxicity Information

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:PPFIBP1-ROS1

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: PPFIBP1-ROS1
FusionPDB ID: 67461
FusionGDB2.0 ID: 67461
HgeneTgene
Gene symbol

PPFIBP1

ROS1

Gene ID

8496

6098

Gene namePPFIA binding protein 1ROS proto-oncogene 1, receptor tyrosine kinase
SynonymsL2|SGT2|hSGT2|hSgt2pMCF3|ROS|c-ros-1
Cytomap

12p11.23-p11.22

6q22.1

Type of geneprotein-codingprotein-coding
Descriptionliprin-beta-1PTPRF interacting protein, binding protein 1 (liprin beta 1)PTPRF-interacting protein-binding protein 1liprin related proteinprotein-tyrosine phosphatase receptor-type f polypeptide-interacting protein-binding protein 1proto-oncogene tyrosine-protein kinase ROSROS proto-oncogene 1 , receptor tyrosine kinasec-ros oncogene 1 , receptor tyrosine kinaseproto-oncogene c-Ros-1transmembrane tyrosine-specific protein kinasev-ros avian UR2 sarcoma virus oncogene homolog 1
Modification date2020031320200314
UniProtAcc

Q86W92

P08922

Ensembl transtripts involved in fusion geneENST idsENST00000318304, ENST00000537927, 
ENST00000228425, ENST00000542629, 
ENST00000535047, ENST00000541633, 
ENST00000545334, 		ENST00000368507, 
ENST00000368508, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score20 X 14 X 13=364018 X 20 X 5=1800
# samples 2620
** MAII scorelog2(26/3640*10)=-3.8073549220576
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(20/1800*10)=-3.16992500144231
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: PPFIBP1 [Title/Abstract] AND ROS1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)
Anticipated loss of major functional domain due to fusion event.PPFIBP1-ROS1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
PPFIBP1-ROS1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
PPFIBP1-ROS1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
PPFIBP1-ROS1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
PPFIBP1-ROS1 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneROS1

GO:0001558

regulation of cell growth

16885344

TgeneROS1

GO:0006468

protein phosphorylation

16885344

TgeneROS1

GO:0032006

regulation of TOR signaling

16885344


check buttonFusion gene breakpoints across PPFIBP1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across ROS1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerKB3..PPFIBP1chr12

27808168

+ROS1chr6

117642557

-
ChimerKB3..PPFIBP1chr12

27809663

+ROS1chr6

117642557

-


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000537927PPFIBP1chr1227809663+ENST00000368508ROS1chr6117642557-25019062572308683
ENST00000537927PPFIBP1chr1227809663+ENST00000368507ROS1chr6117642557-25019062572308683
ENST00000318304PPFIBP1chr1227809663+ENST00000368508ROS1chr6117642557-278211872502589779
ENST00000318304PPFIBP1chr1227809663+ENST00000368507ROS1chr6117642557-278211872502589779
ENST00000542629PPFIBP1chr1227809663+ENST00000368508ROS1chr6117642557-267110762322478748
ENST00000542629PPFIBP1chr1227809663+ENST00000368507ROS1chr6117642557-267110762322478748
ENST00000228425PPFIBP1chr1227809663+ENST00000368508ROS1chr6117642557-263910442002446748
ENST00000228425PPFIBP1chr1227809663+ENST00000368507ROS1chr6117642557-263910442002446748

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>67461_67461_1_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000228425_ROS1_chr6_117642557_ENST00000368507_length(amino acids)=748AA_BP=281
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTA
ETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQK
LDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKDELASLKEQLEEKESEVKRLQEKLVCK
MKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEV
YEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGP
LLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFT
TQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKS
RDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEK

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>67461_67461_2_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000228425_ROS1_chr6_117642557_ENST00000368508_length(amino acids)=748AA_BP=281
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTA
ETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQK
LDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKDELASLKEQLEEKESEVKRLQEKLVCK
MKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEV
YEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGP
LLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFT
TQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKS
RDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEK

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>67461_67461_3_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000318304_ROS1_chr6_117642557_ENST00000368507_length(amino acids)=779AA_BP=312
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTA
ETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQK
LDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAKLSSMKIKVGQMQYEKQRMEQKW
ESLKDELASLKEQLEEKESEVKRLQEKLVCKMKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTL
PTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLL
NEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARD
IYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCW
AQEPDQRPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKS

--------------------------------------------------------------

>67461_67461_4_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000318304_ROS1_chr6_117642557_ENST00000368508_length(amino acids)=779AA_BP=312
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTA
ETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQK
LDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAKLSSMKIKVGQMQYEKQRMEQKW
ESLKDELASLKEQLEEKESEVKRLQEKLVCKMKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTL
PTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLL
NEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARD
IYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCW
AQEPDQRPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKS

--------------------------------------------------------------

>67461_67461_5_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000537927_ROS1_chr6_117642557_ENST00000368507_length(amino acids)=683AA_BP=216
MLFKNTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQKLDLMAE
ISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAKLSSMKIKVGQMQYEKQRMEQKWESLKDE
LASLKEQLEEKESEVKRLQEKLVCKMKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEI
ENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYI
ILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDY
YRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQ
RPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGS

--------------------------------------------------------------

>67461_67461_6_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000537927_ROS1_chr6_117642557_ENST00000368508_length(amino acids)=683AA_BP=216
MLFKNTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQKLDLMAE
ISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAKLSSMKIKVGQMQYEKQRMEQKWESLKDE
LASLKEQLEEKESEVKRLQEKLVCKMKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEI
ENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYI
ILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDY
YRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQ
RPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGS

--------------------------------------------------------------

>67461_67461_7_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000542629_ROS1_chr6_117642557_ENST00000368507_length(amino acids)=748AA_BP=281
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTA
ETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQK
LDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKDELASLKEQLEEKESEVKRLQEKLVCK
MKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEV
YEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGP
LLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFT
TQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKS
RDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEK

--------------------------------------------------------------

>67461_67461_8_PPFIBP1-ROS1_PPFIBP1_chr12_27809663_ENST00000542629_ROS1_chr6_117642557_ENST00000368508_length(amino acids)=748AA_BP=281
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTA
ETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELLSRTSLETQK
LDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKDELASLKEQLEEKESEVKRLQEKLVCK
MKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEV
YEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGP
LLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFT
TQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKS
RDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEK

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:/chr6:)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
PPFIBP1

Q86W92

ROS1

P08922

FUNCTION: May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A. {ECO:0000269|PubMed:9624153}.FUNCTION: Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. {ECO:0000269|PubMed:11094073, ECO:0000269|PubMed:16885344}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file (553) >>>553.pdbFusion protein BP residue: 216
CIF file (553) >>>553.cif		PPFIBP1chr1227809663+ROS1chr6117642557-
MLFKNTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIR
DLEFCLEEHREKVNATEEMLQQELLSRTSLETQKLDLMAEISNLKLKLTA
VEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLM
AKLSSMKIKVGQMQYEKQRMEQKWESLKDELASLKEQLEEKESEVKRLQE
KLVCKMKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAA
GVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTA
VDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLG
VCLLNEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDIS
KGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDY
YRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPA
HSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQ
LQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALM
ETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKD
683
3D view using mol* of 553 (AA BP:216)
PDB file (610) >>>610.pdbFusion protein BP residue: 281
CIF file (610) >>>610.cif		PPFIBP1chr1227809663+ROS1chr6117642557-
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPT
SPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTAETLVEWLQSQ
MTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEF
CLEEHREKVNATEEMLQQELLSRTSLETQKLDLMAEISNLKLKLTAVEKD
RLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKDELASLK
EQLEEKESEVKRLQEKLVCKMKGEGVEIVDRVWHRRLKNQKSAKEGVTVL
INEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRL
LLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAH
LMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGP
LLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVK
IGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGI
LIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWA
QEPDQRPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGD
VICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESES
748
3D view using mol* of 610 (AA BP:281)
PDB file (638) >>>638.pdbFusion protein BP residue: 312
CIF file (638) >>>638.cif		PPFIBP1chr1227809663+ROS1chr6117642557-
MGWNLPLTNNKMMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPT
SPFMGSLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTAETLVEWLQSQ
MTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEF
CLEEHREKVNATEEMLQQELLSRTSLETQKLDLMAEISNLKLKLTAVEKD
RLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAKLS
SMKIKVGQMQYEKQRMEQKWESLKDELASLKEQLEEKESEVKRLQEKLVC
KMKGEGVEIVDRVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGL
ANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDIL
GVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLL
NEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCV
YLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKR
GEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNL
DVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLF
RNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKN
REGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQE
779
3D view using mol* of 638 (AA BP:312)


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
PPFIBP1_pLDDT.png
all structure
ROS1_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
PPFIBP1_ROS1_553_pLDDT.png (AA BP:216)
all structure
PPFIBP1_ROS1_553_pLDDT_and_active_sites.png (AA BP:216)
all structure
PPFIBP1_ROS1_553_violinplot.png (AA BP:216)
all structure
PPFIBP1_ROS1_610_pLDDT.png (AA BP:281)
all structure
PPFIBP1_ROS1_610_pLDDT_and_active_sites.png (AA BP:281)
all structure
PPFIBP1_ROS1_610_violinplot.png (AA BP:281)
all structure
PPFIBP1_ROS1_638_pLDDT.png (AA BP:312)
all structure
PPFIBP1_ROS1_638_pLDDT_and_active_sites.png (AA BP:312)
all structure
PPFIBP1_ROS1_638_violinplot.png (AA BP:312)
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots
PPFIBP1_ROS1_638.png
all structure

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Potential Active Site Information


check button The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.
Fusion AA seq ID in FusionPDBSite scoreSizeD scoreVolumeExposureEnclosureContactPhobicPhilicBalanceDon/AccResidues
5531.0842011.083521.360.3980.8241.1440.6391.0780.5921.076Chain A: 252,253,254,255,256,257,258,259,260,261,2
92,316,326,329,330,332,333,336,411,413,414,415,420
,438,439,440,441,443,450,451,453,456,457,458,459,4
60,461,464,468,469,470,471,472,474,503,504,507
6101.1003091821.000484404.740.4129030.8479891.1249320.3918681.3751820.2849570.747741Chain A: 317,318,319,320,321,322,323,324,325,357,3
94,397,398,401,476,477,478,479,480,485,503,504,505
,506,507,508,515,518,521,522,523,524,525,529,537,5
39
6381.1331331.187377.9860.4480.8061.0361.6990.7272.3371.23Chain A: 383,384,385,386,387,388,389,391,393,410,4
12,429,433,442,458,459,460,461,462,464,465,515,516
,518,522,533,534,536,537,538,541,542

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Potentially Interacting Small Molecules through Virtual Screening


check button The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.
Fusion AA seq ID in FusionPDBZINC IDDrugBank IDDrug nameDocking scoreGlide gscore

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check button Drug information from DrugBank of the top 20 interacting small molecules.
ZINC IDDrugBank IDDrug nameDrug typeSMILESDrug group

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Biochemical Features of Small Molecules


check button ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)
ZINC IDmol_MWdipoleSASAFOSAFISAPISAWPSAvolumedonorHBaccptHBIPHuman Oral AbsorptionPercent Human Oral AbsorptionRule Of FiveRule Of Three


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Drug Toxicity Information


check button Toxicity information of individual drugs using eToxPred
ZINC IDSmileSurface AccessibilityToxicity


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
PPFIBP1YWHAG, YWHAQ, SFN, S100A4, PPFIBP1, ELAVL1, SH3KBP1, GRK5, LATS2, YWHAB, CDKN1A, CEP250, CEP76, TP53, VCP, ENO1, MOV10, NXF1, PNKD, NTRK1, PPFIA1, PPFIA2, PPFIA3, TMEM17, PPP2R1A, DYNLL1, KIF21A, Myh9, Myo1c, Flnb, Scai, YWHAZ, CDH1, HAO2, RAPGEF5, KANK2, DCAF7, EFTUD2, ESR2, HRAS, KRAS, NRAS, LAMP1, KIAA1429, RPS6KA2, BICD1, TNFSF13B, ETAA1, PBX2, OBSL1, MAGEA3, PRKCZ, P4HB, PRRC2C, ESR1, ANLN, KIF14, KIF20A, nsp16, HULC, DNAJC5B, DNAJC5, PRKACA, ANK3, ARF6, B3GAT1, C11orf52, CAV1, CXADR, DIRAS3, EBAG9, EPB41L4A, EZR, FLOT1, GJA1, GJD3, LAMP2, LAMP3, LAMTOR1, LCK, LMAN1, LYN, MARCKS, MLLT4, OCLN, PANX1, PARD3, RAB11A, RAB2A, RAB35, RAB5A, RAB5C, RAB9A, RHOB, STX4, STX6, STX7, ZFPL1, VPS33B, SMAD4, YWHAH, DYNLL2, S100A6, S100B, TACR3, AKR7L, BTNL9, MFNG, GPR17, PPFIBP2, HSF2BP, GCGR, METTL21B, SNAP29, NPAS1, INF2, UBXN6, EMID1, PLBD1, MAB21L2, CPLX2, CHCHD4, SERBP1, MEIS2, BIRC8, COMTD1, SPN, MUM1, PSG11, BTF3, TOLLIP,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
PPFIBP1all structure
ROS1all structure


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to PPFIBP1-ROS1


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to PPFIBP1-ROS1


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID
PPFIBP1ROS1Lung AdenocarcinomaMyCancerGenome
PPFIBP1ROS1Dedifferentiated LiposarcomaMyCancerGenome
PPFIBP1ROS1Breast Invasive Ductal CarcinomaMyCancerGenome
PPFIBP1ROS1Anaplastic GangliogliomaMyCancerGenome
PPFIBP1ROS1Cancer Of Unknown PrimaryMyCancerGenome

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgenePPFIBP1C0085269Plasma Cell Granuloma, Pulmonary1CTD_human
TgeneROS1C0007131Non-Small Cell Lung Carcinoma4CTD_human
TgeneROS1C0017638Glioma1CTD_human
TgeneROS1C0025202melanoma1CTD_human
TgeneROS1C0152013Adenocarcinoma of lung (disorder)1CGI;CTD_human
TgeneROS1C0259783mixed gliomas1CTD_human
TgeneROS1C0555198Malignant Glioma1CTD_human