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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:PRRG1-TSPAN7

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: PRRG1-TSPAN7
	FusionPDB ID: 69265
	FusionGDB2.0 ID: 69265
		Hgene	Tgene
	Gene symbol	PRRG1	TSPAN7
	Gene ID	5638	7102
	Gene name	proline rich and Gla domain 1	tetraspanin 7
	Synonyms	PRGP1	A15\|CCG-B7\|CD231\|DXS1692E\|MRX58\|MXS1\|TALLA-1\|TM4SF2\|TM4SF2b
	Cytomap	Xp21.1	Xp11.4
	Type of gene	protein-coding	protein-coding
	Description	transmembrane gamma-carboxyglutamic acid protein 1proline rich Gla (G-carboxyglutamic acid) 1proline-rich Gla (G-carboxyglutamic acid) polypeptide 1	tetraspanin-7CD231 antigenT-cell acute lymphoblastic leukemia associated antigen 1cell surface glycoprotein A15membrane component chromosome X surface marker 1membrane component, X chromosome, surface marker 1tetraspanin proteintransmembrane 4 supe
	Modification date	20200313	20200320
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000378628, ENST00000449135, ENST00000463135, ENST00000542554, ENST00000543642, ENST00000491253,	ENST00000488893, ENST00000286824, ENST00000378482, ENST00000422612, ENST00000545599,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	3 X 2 X 2=12	11 X 10 X 4=440
	# samples	3	12
	** MAII score	log2(3/12*10)=1.32192809488736 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0	log2(12/440*10)=-1.87446911791614 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: PRRG1 [Title/Abstract] AND TSPAN7 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	PRRG1(37285253)-TSPAN7(38525375), # samples:2
Anticipated loss of major functional domain due to fusion event.	PRRG1-TSPAN7 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PRRG1-TSPAN7 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across PRRG1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TSPAN7 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChiTaRS5.0	N/A	AK303697	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+
ChiTaRS5.0	N/A	DC411619	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000378628	PRRG1	chrX	37285253	+	ENST00000378482	TSPAN7	chrX	38525375	+	1984	333	162	1001	279
ENST00000378628	PRRG1	chrX	37285253	+	ENST00000422612	TSPAN7	chrX	38525375	+	1239	333	162	1001	279
ENST00000378628	PRRG1	chrX	37285253	+	ENST00000545599	TSPAN7	chrX	38525375	+	1342	333	162	1001	279
ENST00000378628	PRRG1	chrX	37285253	+	ENST00000286824	TSPAN7	chrX	38525375	+	1468	333	162	1001	279
ENST00000543642	PRRG1	chrX	37285253	+	ENST00000378482	TSPAN7	chrX	38525375	+	2003	352	181	1020	279
ENST00000543642	PRRG1	chrX	37285253	+	ENST00000422612	TSPAN7	chrX	38525375	+	1258	352	181	1020	279
ENST00000543642	PRRG1	chrX	37285253	+	ENST00000545599	TSPAN7	chrX	38525375	+	1361	352	181	1020	279
ENST00000543642	PRRG1	chrX	37285253	+	ENST00000286824	TSPAN7	chrX	38525375	+	1487	352	181	1020	279
ENST00000542554	PRRG1	chrX	37285253	+	ENST00000378482	TSPAN7	chrX	38525375	+	2094	443	194	1111	305
ENST00000542554	PRRG1	chrX	37285253	+	ENST00000422612	TSPAN7	chrX	38525375	+	1349	443	194	1111	305
ENST00000542554	PRRG1	chrX	37285253	+	ENST00000545599	TSPAN7	chrX	38525375	+	1452	443	194	1111	305
ENST00000542554	PRRG1	chrX	37285253	+	ENST00000286824	TSPAN7	chrX	38525375	+	1578	443	194	1111	305
ENST00000449135	PRRG1	chrX	37285253	+	ENST00000378482	TSPAN7	chrX	38525375	+	1978	327	78	995	305
ENST00000449135	PRRG1	chrX	37285253	+	ENST00000422612	TSPAN7	chrX	38525375	+	1233	327	78	995	305
ENST00000449135	PRRG1	chrX	37285253	+	ENST00000545599	TSPAN7	chrX	38525375	+	1336	327	78	995	305
ENST00000449135	PRRG1	chrX	37285253	+	ENST00000286824	TSPAN7	chrX	38525375	+	1462	327	78	995	305
ENST00000463135	PRRG1	chrX	37285253	+	ENST00000378482	TSPAN7	chrX	38525375	+	1880	229	58	897	279
ENST00000463135	PRRG1	chrX	37285253	+	ENST00000422612	TSPAN7	chrX	38525375	+	1135	229	58	897	279
ENST00000463135	PRRG1	chrX	37285253	+	ENST00000545599	TSPAN7	chrX	38525375	+	1238	229	58	897	279
ENST00000463135	PRRG1	chrX	37285253	+	ENST00000286824	TSPAN7	chrX	38525375	+	1364	229	58	897	279

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000378628	ENST00000378482	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.000761628	0.9992384
ENST00000378628	ENST00000422612	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.002133876	0.9978661
ENST00000378628	ENST00000545599	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.00220443	0.9977956
ENST00000378628	ENST00000286824	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001887849	0.9981122
ENST00000543642	ENST00000378482	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.000587173	0.9994129
ENST00000543642	ENST00000422612	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001722642	0.9982773
ENST00000543642	ENST00000545599	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001897852	0.9981021
ENST00000543642	ENST00000286824	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001697327	0.9983027
ENST00000542554	ENST00000378482	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.000769286	0.99923074
ENST00000542554	ENST00000422612	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.00212207	0.99787796
ENST00000542554	ENST00000545599	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.002432301	0.9975677
ENST00000542554	ENST00000286824	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.002094221	0.99790585
ENST00000449135	ENST00000378482	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.00063353	0.99936646
ENST00000449135	ENST00000422612	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001601529	0.9983985
ENST00000449135	ENST00000545599	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001733623	0.9982664
ENST00000449135	ENST00000286824	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001540507	0.99845946
ENST00000463135	ENST00000378482	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.000471641	0.9995284
ENST00000463135	ENST00000422612	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.00116343	0.9988366
ENST00000463135	ENST00000545599	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001261568	0.99873847
ENST00000463135	ENST00000286824	PRRG1	chrX	37285253	+	TSPAN7	chrX	38525375	+	0.001129778	0.9988702

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>69265_69265_1_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000378628_TSPAN7_chrX_38525375_ENST00000286824_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_2_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000378628_TSPAN7_chrX_38525375_ENST00000378482_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_3_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000378628_TSPAN7_chrX_38525375_ENST00000422612_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_4_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000378628_TSPAN7_chrX_38525375_ENST00000545599_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_5_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000449135_TSPAN7_chrX_38525375_ENST00000286824_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_6_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000449135_TSPAN7_chrX_38525375_ENST00000378482_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_7_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000449135_TSPAN7_chrX_38525375_ENST00000422612_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_8_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000449135_TSPAN7_chrX_38525375_ENST00000545599_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_9_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000463135_TSPAN7_chrX_38525375_ENST00000286824_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_10_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000463135_TSPAN7_chrX_38525375_ENST00000378482_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_11_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000463135_TSPAN7_chrX_38525375_ENST00000422612_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_12_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000463135_TSPAN7_chrX_38525375_ENST00000545599_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_13_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000542554_TSPAN7_chrX_38525375_ENST00000286824_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_14_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000542554_TSPAN7_chrX_38525375_ENST00000378482_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_15_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000542554_TSPAN7_chrX_38525375_ENST00000422612_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_16_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000542554_TSPAN7_chrX_38525375_ENST00000545599_length(amino acids)=305AA_BP=83
MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL
AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY
TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG

--------------------------------------------------------------

>69265_69265_17_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000543642_TSPAN7_chrX_38525375_ENST00000286824_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_18_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000543642_TSPAN7_chrX_38525375_ENST00000378482_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_19_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000543642_TSPAN7_chrX_38525375_ENST00000422612_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

--------------------------------------------------------------

>69265_69265_20_PRRG1-TSPAN7_PRRG1_chrX_37285253_ENST00000543642_TSPAN7_chrX_38525375_ENST00000545599_length(amino acids)=279AA_BP=57
MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILLAVGVWGKLTLGTYISLIAENSTNAPY
VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSC
CGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSRF

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chrX:37285253/chrX:38525375)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

113_213

27.0

489.0

Topological domain

Extracellular

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

235_249

27.0

489.0

Topological domain

Cytoplasmic

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

41_56

27.0

489.0

Topological domain

Extracellular

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

76_86

27.0

489.0

Topological domain

Cytoplasmic

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

214_234

27.0

489.0

Transmembrane

Helical

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

57_75

27.0

489.0

Transmembrane

Helical

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

87_112

27.0

489.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000378628

131_135

57.0

219.0

Compositional bias

Note=Poly-Pro

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000449135

131_135

57.0

219.0

Compositional bias

Note=Poly-Pro

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000463135

131_135

57.0

83.0

Compositional bias

Note=Poly-Pro

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000542554

131_135

57.0

219.0

Compositional bias

Note=Poly-Pro

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000543642

131_135

57.0

219.0

Compositional bias

Note=Poly-Pro

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000378628

21_66

57.0

219.0

Domain

Gla

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000449135

21_66

57.0

219.0

Domain

Gla

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000463135

21_66

57.0

83.0

Domain

Gla

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000542554

21_66

57.0

219.0

Domain

Gla

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000543642

21_66

57.0

219.0

Domain

Gla

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000378628

107_218

57.0

219.0

Topological domain

Cytoplasmic

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000378628

21_83

57.0

219.0

Topological domain

Extracellular

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000449135

107_218

57.0

219.0

Topological domain

Cytoplasmic

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000449135

21_83

57.0

219.0

Topological domain

Extracellular

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000463135

107_218

57.0

83.0

Topological domain

Cytoplasmic

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000463135

21_83

57.0

83.0

Topological domain

Extracellular

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000542554

107_218

57.0

219.0

Topological domain

Cytoplasmic

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000542554

21_83

57.0

219.0

Topological domain

Extracellular

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000543642

107_218

57.0

219.0

Topological domain

Cytoplasmic

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000543642

21_83

57.0

219.0

Topological domain

Extracellular

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000378628

84_106

57.0

219.0

Transmembrane

Helical

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000449135

84_106

57.0

219.0

Transmembrane

Helical

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000463135

84_106

57.0

83.0

Transmembrane

Helical

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000542554

84_106

57.0

219.0

Transmembrane

Helical

Hgene

PRRG1

chrX:37285253

chrX:38525375

ENST00000543642

84_106

57.0

219.0

Transmembrane

Helical

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

1_16

27.0

489.0

Topological domain

Cytoplasmic

Tgene

TSPAN7

chrX:37285253

chrX:38525375

ENST00000378482

17_40

27.0

489.0

Transmembrane

Helical

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>554_PRRG1_37285253_TSPAN7_38525375_ranked_0.pdb	PRRG1	37285253	37285253	ENST00000286824	TSPAN7	chrX	38525375	+	MLLYIIIEPDYLGNHHPGTCQKPQENMGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTITGVILL AVGVWGKLTLGTYISLIAENSTNAPYVLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTY TDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNMG	305

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

PRRG1_pLDDT.png

TSPAN7_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
PRRG1
TSPAN7

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to PRRG1-TSPAN7

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to PRRG1-TSPAN7

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source