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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:PUM1-SDCCAG8

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: PUM1-SDCCAG8
	FusionPDB ID: 70689
	FusionGDB2.0 ID: 70689
		Hgene	Tgene
	Gene symbol	PUM1	SDCCAG8
	Gene ID	9698	10806
	Gene name	pumilio RNA binding family member 1	SHH signaling and ciliogenesis regulator SDCCAG8
	Synonyms	HSPUM\|PUMH\|PUMH1\|PUML1\|SCA47	BBS16\|CCCAP\|CCCAP SLSN7\|HSPC085\|NPHP10\|NY-CO-8\|SLSN7\|hCCCAP
	Cytomap	1p35.2	1q43-q44
	Type of gene	protein-coding	protein-coding
	Description	pumilio homolog 1pumilio-1	serologically defined colon cancer antigen 8Bardet-Biedl syndrome 16antigen NY-CO-8centrosomal colon cancer autoantigen proteinnephrocystin 10
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000257075, ENST00000373741, ENST00000373742, ENST00000373747, ENST00000423018, ENST00000424085, ENST00000426105, ENST00000440538, ENST00000490546,	ENST00000391846, ENST00000496361, ENST00000343783, ENST00000355875, ENST00000366541,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	38 X 31 X 15=17670	22 X 19 X 10=4180
	# samples	56	26
	** MAII score	log2(56/17670*10)=-4.97973140249401 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(26/4180*10)=-4.00691941393979 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: PUM1 [Title/Abstract] AND SDCCAG8 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	PUM1(31501643)-SDCCAG8(243493842), # samples:3
Anticipated loss of major functional domain due to fusion event.	PUM1-SDCCAG8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PUM1-SDCCAG8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PUM1-SDCCAG8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. PUM1-SDCCAG8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. PUM1-SDCCAG8 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	PUM1	GO:0010608	posttranscriptional regulation of gene expression	25100735
Hgene	PUM1	GO:0043488	regulation of mRNA stability	26724866
Hgene	PUM1	GO:0051726	regulation of cell cycle	20818387
Hgene	PUM1	GO:0051983	regulation of chromosome segregation	26724866
Hgene	PUM1	GO:1900246	positive regulation of RIG-I signaling pathway	25340845
Hgene	PUM1	GO:2000637	positive regulation of gene silencing by miRNA	20818387\|22345517

Fusion gene breakpoints across PUM1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across SDCCAG8 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-EJ-5527-01A	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+
ChimerDB4	PRAD	TCGA-EJ-5527	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000373741	PUM1	chr1	31501643	-	ENST00000355875	SDCCAG8	chr1	243493842	+	2121	746	113	1819	568
ENST00000373741	PUM1	chr1	31501643	-	ENST00000366541	SDCCAG8	chr1	243493842	+	2127	746	113	1819	568
ENST00000373741	PUM1	chr1	31501643	-	ENST00000343783	SDCCAG8	chr1	243493842	+	2125	746	113	1819	568
ENST00000373742	PUM1	chr1	31501643	-	ENST00000355875	SDCCAG8	chr1	243493842	+	1915	540	0	1613	537
ENST00000373742	PUM1	chr1	31501643	-	ENST00000366541	SDCCAG8	chr1	243493842	+	1921	540	0	1613	537
ENST00000373742	PUM1	chr1	31501643	-	ENST00000343783	SDCCAG8	chr1	243493842	+	1919	540	0	1613	537

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000373741	ENST00000355875	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+	0.010137218	0.9898628
ENST00000373741	ENST00000366541	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+	0.009734445	0.99026555
ENST00000373741	ENST00000343783	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+	0.009774008	0.990226
ENST00000373742	ENST00000355875	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+	0.011145706	0.9888543
ENST00000373742	ENST00000366541	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+	0.010689452	0.9893106
ENST00000373742	ENST00000343783	PUM1	chr1	31501643	-	SDCCAG8	chr1	243493842	+	0.010698572	0.9893014

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>70689_70689_1_PUM1-SDCCAG8_PUM1_chr1_31501643_ENST00000373741_SDCCAG8_chr1_243493842_ENST00000343783_length(amino acids)=568AA_BP=211
MCCLRVSRVHTGPGLAWQPGNLFQSRQPSPHMPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLK
HHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTG
DNIHAEHQVRSMDELNHDFQALALEGRAMGEALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNI
AQLEAQVEKVTKEKISAINQLEEIQSQLASREMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQ
HLEQEQQKAALAREECLRLTELLGESEHQLHLTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTF
LTKLKEECCTLAKKLEQISQKTRSEIAQLSQEKRYTYDKLGKLQRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQATAQQLVQLLSKQN

--------------------------------------------------------------

>70689_70689_2_PUM1-SDCCAG8_PUM1_chr1_31501643_ENST00000373741_SDCCAG8_chr1_243493842_ENST00000355875_length(amino acids)=568AA_BP=211
MCCLRVSRVHTGPGLAWQPGNLFQSRQPSPHMPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLK
HHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTG
DNIHAEHQVRSMDELNHDFQALALEGRAMGEALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNI
AQLEAQVEKVTKEKISAINQLEEIQSQLASREMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQ
HLEQEQQKAALAREECLRLTELLGESEHQLHLTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTF
LTKLKEECCTLAKKLEQISQKTRSEIAQLSQEKRYTYDKLGKLQRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQATAQQLVQLLSKQN

--------------------------------------------------------------

>70689_70689_3_PUM1-SDCCAG8_PUM1_chr1_31501643_ENST00000373741_SDCCAG8_chr1_243493842_ENST00000366541_length(amino acids)=568AA_BP=211
MCCLRVSRVHTGPGLAWQPGNLFQSRQPSPHMPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLK
HHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTG
DNIHAEHQVRSMDELNHDFQALALEGRAMGEALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNI
AQLEAQVEKVTKEKISAINQLEEIQSQLASREMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQ
HLEQEQQKAALAREECLRLTELLGESEHQLHLTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTF
LTKLKEECCTLAKKLEQISQKTRSEIAQLSQEKRYTYDKLGKLQRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQATAQQLVQLLSKQN

--------------------------------------------------------------

>70689_70689_4_PUM1-SDCCAG8_PUM1_chr1_31501643_ENST00000373742_SDCCAG8_chr1_243493842_ENST00000343783_length(amino acids)=537AA_BP=180
MPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQ
ALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGE
ALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNIAQLEAQVEKVTKEKISAINQLEEIQSQLASR
EMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQHLEQEQQKAALAREECLRLTELLGESEHQLH
LTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTFLTKLKEECCTLAKKLEQISQKTRSEIAQLSQ

--------------------------------------------------------------

>70689_70689_5_PUM1-SDCCAG8_PUM1_chr1_31501643_ENST00000373742_SDCCAG8_chr1_243493842_ENST00000355875_length(amino acids)=537AA_BP=180
MPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQ
ALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGE
ALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNIAQLEAQVEKVTKEKISAINQLEEIQSQLASR
EMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQHLEQEQQKAALAREECLRLTELLGESEHQLH
LTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTFLTKLKEECCTLAKKLEQISQKTRSEIAQLSQ

--------------------------------------------------------------

>70689_70689_6_PUM1-SDCCAG8_PUM1_chr1_31501643_ENST00000373742_SDCCAG8_chr1_243493842_ENST00000366541_length(amino acids)=537AA_BP=180
MPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQ
ALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGE
ALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNIAQLEAQVEKVTKEKISAINQLEEIQSQLASR
EMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQHLEQEQQKAALAREECLRLTELLGESEHQLH
LTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTFLTKLKEECCTLAKKLEQISQKTRSEIAQLSQ

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:31501643/chr1:243493842)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000391846

129_175

361.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000391846

223_273

361.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000391846

348_707

361.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000366541

534_692

356.0

714.0

Compositional bias

Note=Gln-rich

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000391846

534_692

361.0

Compositional bias

Note=Gln-rich

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000391846

216_713

361.0

Region

Sufficient for homodimerization

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

393_613

144.0

1187.0

Compositional bias

Note=Ala-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

475_523

144.0

1187.0

Compositional bias

Note=Gln-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

642_815

144.0

1187.0

Compositional bias

Note=Ser-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

393_613

144.0

1189.0

Compositional bias

Note=Ala-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

475_523

144.0

1189.0

Compositional bias

Note=Gln-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

642_815

144.0

1189.0

Compositional bias

Note=Ser-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

393_613

144.0

1163.0

Compositional bias

Note=Ala-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

475_523

144.0

1163.0

Compositional bias

Note=Gln-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

642_815

144.0

1163.0

Compositional bias

Note=Ser-rich

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

828_1168

144.0

1187.0

Domain

PUM-HD

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

828_1168

144.0

1189.0

Domain

PUM-HD

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

828_1168

144.0

1163.0

Domain

PUM-HD

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

1028_1063

144.0

1187.0

Repeat

Pumilio 6

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

1064_1099

144.0

1187.0

Repeat

Pumilio 7

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

1103_1142

144.0

1187.0

Repeat

Pumilio 8

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

848_883

144.0

1187.0

Repeat

Pumilio 1

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

884_919

144.0

1187.0

Repeat

Pumilio 2

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

920_955

144.0

1187.0

Repeat

Pumilio 3

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

956_991

144.0

1187.0

Repeat

Pumilio 4

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000257075

992_1027

144.0

1187.0

Repeat

Pumilio 5

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

1028_1063

144.0

1189.0

Repeat

Pumilio 6

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

1064_1099

144.0

1189.0

Repeat

Pumilio 7

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

1103_1142

144.0

1189.0

Repeat

Pumilio 8

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

848_883

144.0

1189.0

Repeat

Pumilio 1

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

884_919

144.0

1189.0

Repeat

Pumilio 2

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

920_955

144.0

1189.0

Repeat

Pumilio 3

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

956_991

144.0

1189.0

Repeat

Pumilio 4

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000426105

992_1027

144.0

1189.0

Repeat

Pumilio 5

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

1028_1063

144.0

1163.0

Repeat

Pumilio 6

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

1064_1099

144.0

1163.0

Repeat

Pumilio 7

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

1103_1142

144.0

1163.0

Repeat

Pumilio 8

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

848_883

144.0

1163.0

Repeat

Pumilio 1

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

884_919

144.0

1163.0

Repeat

Pumilio 2

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

920_955

144.0

1163.0

Repeat

Pumilio 3

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

956_991

144.0

1163.0

Repeat

Pumilio 4

Hgene

PUM1

chr1:31501643

chr1:243493842

ENST00000440538

992_1027

144.0

1163.0

Repeat

Pumilio 5

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000366541

129_175

356.0

714.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000366541

223_273

356.0

714.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000366541

348_707

356.0

714.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

SDCCAG8

chr1:31501643

chr1:243493842

ENST00000366541

216_713

356.0

714.0

Region

Sufficient for homodimerization

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1214_PUM1_31501643_SDCCAG8_243493842_1214_PUM1_31501643_SDCCAG8_243493842_ranked_0.pdb	PUM1	31501643	31501643	ENST00000343783	SDCCAG8	chr1	243493842	+	MCCLRVSRVHTGPGLAWQPGNLFQSRQPSPHMPLPPPGPGPEPIPGCTAPTQSPVGRHVVGVKGVGGMSVACVLKRKAVLWQDSFSPHLK HHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTG DNIHAEHQVRSMDELNHDFQALALEGRAMGEALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNI AQLEAQVEKVTKEKISAINQLEEIQSQLASREMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQ HLEQEQQKAALAREECLRLTELLGESEHQLHLTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTF LTKLKEECCTLAKKLEQISQKTRSEIAQLSQEKRYTYDKLGKLQRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQATAQQLVQLLSKQN	568

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

PUM1_pLDDT.png

SDCCAG8_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
PUM1
SDCCAG8

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to PUM1-SDCCAG8

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to PUM1-SDCCAG8

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source