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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:RAD51B-MPP5

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RAD51B-MPP5
	FusionPDB ID: 71751
	FusionGDB2.0 ID: 71751
		Hgene	Tgene
	Gene symbol	RAD51B	MPP5
	Gene ID	5890	64398
	Gene name	RAD51 paralog B	membrane palmitoylated protein 5
	Synonyms	R51H2\|RAD51L1\|REC2	PALS1
	Cytomap	14q24.1	14q23.3
	Type of gene	protein-coding	protein-coding
	Description	DNA repair protein RAD51 homolog 2RAD51 homolog BRecA-like proteinrecombination repair protein	MAGUK p55 subfamily member 5membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)protein associated with Lin-7 1protein associated with lin seven 1stardust
	Modification date	20200313	20200313
	UniProtAcc	O15315	Q8N3R9
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000469165, ENST00000390683, ENST00000471583, ENST00000487270, ENST00000487861, ENST00000488612,	ENST00000261681, ENST00000555925, ENST00000554911, ENST00000556345,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	22 X 15 X 11=3630	5 X 7 X 6=210
	# samples	22	8
	** MAII score	log2(22/3630*10)=-4.04439411935845 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/210*10)=-1.39231742277876 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: RAD51B [Title/Abstract] AND MPP5 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MPP5(67779427)-RAD51B(69196525), # samples:2 RAD51B(68353921)-MPP5(67770238), # samples:2
Anticipated loss of major functional domain due to fusion event.	MPP5-RAD51B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MPP5-RAD51B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RAD51B-MPP5 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RAD51B-MPP5 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RAD51B-MPP5 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RAD51B-MPP5 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across RAD51B (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across MPP5 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	OV	TCGA-24-2026	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+
ChimerDB4	UCEC	TCGA-B5-A11J	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000487861	RAD51B	chr14	68353921	+	ENST00000261681	MPP5	chr14	67770238	+	4761	833	29	1897	622
ENST00000487861	RAD51B	chr14	68353921	+	ENST00000555925	MPP5	chr14	67770238	+	4761	833	29	1897	622
ENST00000471583	RAD51B	chr14	68353921	+	ENST00000261681	MPP5	chr14	67770238	+	4732	804	0	1868	622
ENST00000471583	RAD51B	chr14	68353921	+	ENST00000555925	MPP5	chr14	67770238	+	4732	804	0	1868	622
ENST00000487270	RAD51B	chr14	68353921	+	ENST00000261681	MPP5	chr14	67770238	+	4732	804	0	1868	622
ENST00000487270	RAD51B	chr14	68353921	+	ENST00000555925	MPP5	chr14	67770238	+	4732	804	0	1868	622
ENST00000488612	RAD51B	chr14	68353921	+	ENST00000261681	MPP5	chr14	67770238	+	4711	783	0	1847	615
ENST00000488612	RAD51B	chr14	68353921	+	ENST00000555925	MPP5	chr14	67770238	+	4711	783	0	1847	615
ENST00000390683	RAD51B	chr14	68353921	+	ENST00000261681	MPP5	chr14	67770238	+	4684	756	0	1820	606
ENST00000390683	RAD51B	chr14	68353921	+	ENST00000555925	MPP5	chr14	67770238	+	4684	756	0	1820	606

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000487861	ENST00000261681	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000167368	0.99983263
ENST00000487861	ENST00000555925	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000167368	0.99983263
ENST00000471583	ENST00000261681	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000167989	0.99983203
ENST00000471583	ENST00000555925	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000167989	0.99983203
ENST00000487270	ENST00000261681	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000167989	0.99983203
ENST00000487270	ENST00000555925	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000167989	0.99983203
ENST00000488612	ENST00000261681	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000174262	0.9998258
ENST00000488612	ENST00000555925	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000174262	0.9998258
ENST00000390683	ENST00000261681	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000168323	0.9998317
ENST00000390683	ENST00000555925	RAD51B	chr14	68353921	+	MPP5	chr14	67770238	+	0.000168323	0.9998317

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>71751_71751_1_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000390683_MPP5_chr14_67770238_ENST00000261681_length(amino acids)=606AA_BP=252
MGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQRSADFSPAFLSTTLSAL
DEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYFNTEEKLLLTSSKVHLY
RELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSDMHGTLTFVLIPSQQIK
PPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEP
EKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASAVPHTTRSRR
DQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALL

--------------------------------------------------------------

>71751_71751_2_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000390683_MPP5_chr14_67770238_ENST00000555925_length(amino acids)=606AA_BP=252
MGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQRSADFSPAFLSTTLSAL
DEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYFNTEEKLLLTSSKVHLY
RELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSDMHGTLTFVLIPSQQIK
PPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEP
EKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASAVPHTTRSRR
DQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALL

--------------------------------------------------------------

>71751_71751_3_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000471583_MPP5_chr14_67770238_ENST00000261681_length(amino acids)=622AA_BP=268
LCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR
SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF
NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD
MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ
QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE
KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY

--------------------------------------------------------------

>71751_71751_4_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000471583_MPP5_chr14_67770238_ENST00000555925_length(amino acids)=622AA_BP=268
LCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR
SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF
NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD
MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ
QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE
KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY

--------------------------------------------------------------

>71751_71751_5_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000487270_MPP5_chr14_67770238_ENST00000261681_length(amino acids)=622AA_BP=268
LCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR
SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF
NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD
MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ
QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE
KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY

--------------------------------------------------------------

>71751_71751_6_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000487270_MPP5_chr14_67770238_ENST00000555925_length(amino acids)=622AA_BP=268
LCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR
SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF
NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD
MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ
QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE
KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY

--------------------------------------------------------------

>71751_71751_7_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000487861_MPP5_chr14_67770238_ENST00000261681_length(amino acids)=622AA_BP=268
MCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR
SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF
NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD
MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ
QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE
KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY

--------------------------------------------------------------

>71751_71751_8_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000487861_MPP5_chr14_67770238_ENST00000555925_length(amino acids)=622AA_BP=268
MCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR
SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF
NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD
MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ
QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE
KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY

--------------------------------------------------------------

>71751_71751_9_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000488612_MPP5_chr14_67770238_ENST00000261681_length(amino acids)=615AA_BP=261
LKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQRSADFSPA
FLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYFNTEEKLL
LTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSDMHGTLTF
VLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMK
QTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASA
VPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPP

--------------------------------------------------------------

>71751_71751_10_RAD51B-MPP5_RAD51B_chr14_68353921_ENST00000488612_MPP5_chr14_67770238_ENST00000555925_length(amino acids)=615AA_BP=261
LKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQRSADFSPA
FLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYFNTEEKLL
LTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSDMHGTLTF
VLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMK
QTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASA
VPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPP

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:67779427/chr14:69196525)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
RAD51B O15315	MPP5 Q8N3R9
FUNCTION: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. {ECO:0000269\|PubMed:11751635, ECO:0000269\|PubMed:11751636, ECO:0000269\|PubMed:11842113, ECO:0000269\|PubMed:12441335, ECO:0000269\|PubMed:23108668, ECO:0000269\|PubMed:23149936}.	FUNCTION: Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells (PubMed:16678097, PubMed:25385611). Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface (By similarity). Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity (PubMed:27466317). Required during embryonic and postnatal retinal development (By similarity). Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation, cerebellar layer organization and cortical development (By similarity). Plays a role in neuronal progenitor cell survival, potentially via promotion of mTOR signaling (By similarity). Plays a role in the radial and longitudinal extension of the myelin sheath in Schwann cells (By similarity). May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter (By similarity). Plays a role in the T-cell receptor-mediated activation of NF-kappa-B (PubMed:21479189). Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton (By similarity). Required for the normal polarized localization of the vesicular marker STX4 (By similarity). Required for the correct trafficking of the myelin proteins PMP22 and MAG (By similarity). Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7 (By similarity). {ECO:0000250\|UniProtKB:B4F7E7, ECO:0000250\|UniProtKB:Q9JLB2, ECO:0000269\|PubMed:16678097, ECO:0000269\|PubMed:21479189, ECO:0000269\|PubMed:25385611, ECO:0000269\|PubMed:27466317}.; FUNCTION: (Microbial infection) Acts as an interaction partner for human coronaviruses SARS-CoV and, probably, SARS-CoV-2 envelope protein E which results in delayed formation of tight junctions and disregulation of cell polarity. {ECO:0000269\|PubMed:20861307, ECO:0000303\|PubMed:32891874}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RAD51B

chr14:68353921

chr14:67770238

ENST00000390683

108_115

252.0

367.0

Nucleotide binding

ATP

Hgene

RAD51B

chr14:68353921

chr14:67770238

ENST00000471583

108_115

252.0

351.0

Nucleotide binding

ATP

Hgene

RAD51B

chr14:68353921

chr14:67770238

ENST00000487270

108_115

252.0

385.0

Nucleotide binding

ATP

Hgene

RAD51B

chr14:68353921

chr14:67770238

ENST00000488612

108_115

252.0

495.6666666666667

Nucleotide binding

ATP

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

345_417

321.0

676.0

Domain

SH3

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

479_660

321.0

676.0

Domain

Guanylate kinase-like

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

345_417

287.0

642.0

Domain

SH3

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

479_660

287.0

642.0

Domain

Guanylate kinase-like

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

486_493

321.0

676.0

Nucleotide binding

ATP

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

486_493

287.0

642.0

Nucleotide binding

ATP

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

120_177

321.0

676.0

Domain

L27 1

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

179_235

321.0

676.0

Domain

L27 2

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

256_336

321.0

676.0

Domain

PDZ

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

120_177

287.0

642.0

Domain

L27 1

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

179_235

287.0

642.0

Domain

L27 2

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

256_336

287.0

642.0

Domain

PDZ

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000261681

1_345

321.0

676.0

Region

Required for the correct localization of MPP5 and PATJ at cell-cell contacts and the normal formation of tight junctions and adherens junctions

Tgene

MPP5

chr14:68353921

chr14:67770238

ENST00000555925

1_345

287.0

642.0

Region

Required for the correct localization of MPP5 and PATJ at cell-cell contacts and the normal formation of tight junctions and adherens junctions

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1310_RAD51B_68353921_MPP5_67770238_1310_RAD51B_68353921_MPP5_67770238_ranked_0.pdb	RAD51B	68353921	68353921	ENST00000555925	MPP5	chr14	67770238	+	MCKGWGNLKVGCCRPGMGSKKLKRVGLSQELCDRLSRHQILTCQDFLCLSPLELMKVTGLSYRGVHELLCMVSRACAPKMQTAYGIKAQR SADFSPAFLSTTLSALDEALHGGVACGSLTEITGPPGCGKTQFCIMMSILATLPTNMGGLEGAVVYIDTESAFSAERLVEIAESRFPRYF NTEEKLLLTSSKVHLYRELTCDEVLQRIESLEEEIISKGIKLVILDSVASVVRKEFDAQLQGNLKERNKFLAREASSLKYLAEEFSIPSD MHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQ QQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKE KDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPY	622

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

RAD51B_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene	PPI interactors
RAD51B	RAD51, RAD51C, RAD51D, XRCC2, SWSAP1, FAM9B, DKKL1, ARL4C, MUC20, ADAMTS4, HELQ, HNRNPL, SPDL1, BRCA1, TPT1, ABL1, MEOX2, RPAIN, ANAPC5, BAGE2, C15orf39, PRPF6, C5orf34, N4BP2L2, BANP, ANKHD1-EIF4EBP3, KLRC2, DSCC1, SATB2, ZNHIT6, PMS1, NUF2, APAF1, PRKACB, MAPRE2, CD300LB, PRKACG, TBC1D2B, MTR,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
RAD51B
MPP5

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	MPP5	chr14:68353921	chr14:67770238	ENST00000261681		6	15	181_243	321.0	676.0	LIN7C
Tgene	MPP5	chr14:68353921	chr14:67770238	ENST00000555925		6	15	181_243	287.0	642.0	LIN7C
Tgene	MPP5	chr14:68353921	chr14:67770238	ENST00000261681		6	15	21_140	321.0	676.0	PARD6B
Tgene	MPP5	chr14:68353921	chr14:67770238	ENST00000555925		6	15	21_140	287.0	642.0	PARD6B

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Related Drugs to RAD51B-MPP5

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to RAD51B-MPP5

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	RAD51B	C0006142	Malignant neoplasm of breast	1	CTD_human
Hgene	RAD51B	C0013146	Drug abuse	1	CTD_human
Hgene	RAD51B	C0013170	Drug habituation	1	CTD_human
Hgene	RAD51B	C0013222	Drug Use Disorders	1	CTD_human
Hgene	RAD51B	C0029231	Organic Mental Disorders, Substance-Induced	1	CTD_human
Hgene	RAD51B	C0038580	Substance Dependence	1	CTD_human
Hgene	RAD51B	C0038586	Substance Use Disorders	1	CTD_human
Hgene	RAD51B	C0236969	Substance-Related Disorders	1	CTD_human
Hgene	RAD51B	C0238033	Carcinoma of Male Breast	1	CTD_human
Hgene	RAD51B	C0242788	Breast Neoplasms, Male	1	CTD_human
Hgene	RAD51B	C0678222	Breast Carcinoma	1	CTD_human
Hgene	RAD51B	C0740858	Substance abuse problem	1	CTD_human
Hgene	RAD51B	C1257931	Mammary Neoplasms, Human	1	CTD_human
Hgene	RAD51B	C1458155	Mammary Neoplasms	1	CTD_human
Hgene	RAD51B	C1510472	Drug Dependence	1	CTD_human
Hgene	RAD51B	C4316881	Prescription Drug Abuse	1	CTD_human
Hgene	RAD51B	C4704874	Mammary Carcinoma, Human	1	CTD_human