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Center for Computational Systems Medicine level1
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:RASSF3-OS9

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: RASSF3-OS9
FusionPDB ID: 72496
FusionGDB2.0 ID: 72496
HgeneTgene
Gene symbol

RASSF3

OS9

Gene ID

283349

10956

Gene nameRas association domain family member 3OS9 endoplasmic reticulum lectin
SynonymsRASSF5ERLEC2|OS-9
Cytomap

12q14.2

12q13.3-q14.1

Type of geneprotein-codingprotein-coding
Descriptionras association domain-containing protein 3Ras association (RalGDS/AF-6) domain family 3Ras association (RalGDS/AF-6) domain family member 3protein OS-9amplified in osteosarcoma 9endoplasmic reticulum lectin 2erlectin 2osteosarcoma amplified 9, endoplasmic reticulum associated proteinosteosarcoma amplified 9, endoplasmic reticulum lectin
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000540088, ENST00000336061, 
ENST00000542104, 
ENST00000315970, 
ENST00000389146, ENST00000439210, 
ENST00000552285, ENST00000257966, 
ENST00000389142, ENST00000413095, 
ENST00000435406, ENST00000551035, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score18 X 7 X 12=151228 X 16 X 12=5376
# samples 1838
** MAII scorelog2(18/1512*10)=-3.0703893278914
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(38/5376*10)=-3.82246181444781
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: RASSF3 [Title/Abstract] AND OS9 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)RASSF3(65004523)-OS9(58109543), # samples:1
Anticipated loss of major functional domain due to fusion event.RASSF3-OS9 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
RASSF3-OS9 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
RASSF3-OS9 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
RASSF3-OS9 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneOS9

GO:0006621

protein retention in ER lumen

18417469

TgeneOS9

GO:0034976

response to endoplasmic reticulum stress

19084021


check buttonFusion gene breakpoints across RASSF3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across OS9 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SKCMTCGA-GN-A26D-06ARASSF3chr12

65004523

+OS9chr12

58109543

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000336061RASSF3chr1265004523+ENST00000552285OS9chr1258109543+150315411413470
ENST00000336061RASSF3chr1265004523+ENST00000315970OS9chr1258109543+222515411578525
ENST00000336061RASSF3chr1265004523+ENST00000439210OS9chr1258109543+167915411368455
ENST00000336061RASSF3chr1265004523+ENST00000389146OS9chr1258109543+164915411533510
ENST00000542104RASSF3chr1265004523+ENST00000552285OS9chr1258109543+1580231121490492
ENST00000542104RASSF3chr1265004523+ENST00000315970OS9chr1258109543+2302231121655547
ENST00000542104RASSF3chr1265004523+ENST00000439210OS9chr1258109543+1756231121445477
ENST00000542104RASSF3chr1265004523+ENST00000389146OS9chr1258109543+1726231121610532

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000336061ENST00000552285RASSF3chr1265004523+OS9chr1258109543+0.0076707770.9923292
ENST00000336061ENST00000315970RASSF3chr1265004523+OS9chr1258109543+0.0080962610.9919037
ENST00000336061ENST00000439210RASSF3chr1265004523+OS9chr1258109543+0.0070528640.9929471
ENST00000336061ENST00000389146RASSF3chr1265004523+OS9chr1258109543+0.0101727060.9898273
ENST00000542104ENST00000552285RASSF3chr1265004523+OS9chr1258109543+0.0081191270.99188083
ENST00000542104ENST00000315970RASSF3chr1265004523+OS9chr1258109543+0.008600040.9914
ENST00000542104ENST00000439210RASSF3chr1265004523+OS9chr1258109543+0.0077530670.99224687
ENST00000542104ENST00000389146RASSF3chr1265004523+OS9chr1258109543+0.0103466510.9896534

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>72496_72496_1_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000336061_OS9_chr12_58109543_ENST00000315970_length(amino acids)=525AA_BP=50
LHKDCPGLRDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPL
LRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQA
PGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQRE
MEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIK
RLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIE

--------------------------------------------------------------

>72496_72496_2_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000336061_OS9_chr12_58109543_ENST00000389146_length(amino acids)=510AA_BP=50
LHKDCPGLRDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPL
LRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQA
PGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQRE
MEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHK
KKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGA

--------------------------------------------------------------

>72496_72496_3_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000336061_OS9_chr12_58109543_ENST00000439210_length(amino acids)=455AA_BP=50
LHKDCPGLRDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPL
LRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQA
PGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQRE
MEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHK
KKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDL

--------------------------------------------------------------

>72496_72496_4_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000336061_OS9_chr12_58109543_ENST00000552285_length(amino acids)=470AA_BP=50
LHKDCPGLRDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPL
LRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQA
PGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQRE
MEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIK
RLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNY

--------------------------------------------------------------

>72496_72496_5_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000542104_OS9_chr12_58109543_ENST00000315970_length(amino acids)=547AA_BP=72
MPGAARREPRGAGGRRTPSLAACAPGRPPARDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRV
DEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGAS
PTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVD
DAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGL
KKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLK
QIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTG

--------------------------------------------------------------

>72496_72496_6_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000542104_OS9_chr12_58109543_ENST00000389146_length(amino acids)=532AA_BP=72
MPGAARREPRGAGGRRTPSLAACAPGRPPARDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRV
DEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGAS
PTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVD
DAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSETEKELDPDGLKKESERDRAMLALTS
TLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGL

--------------------------------------------------------------

>72496_72496_7_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000542104_OS9_chr12_58109543_ENST00000439210_length(amino acids)=477AA_BP=72
MPGAARREPRGAGGRRTPSLAACAPGRPPARDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRV
DEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGAS
PTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVD
DAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSETEKELDPDGLKKESERDRAMLALTS
TLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQ

--------------------------------------------------------------

>72496_72496_8_RASSF3-OS9_RASSF3_chr12_65004523_ENST00000542104_OS9_chr12_58109543_ENST00000552285_length(amino acids)=492AA_BP=72
MPGAARREPRGAGGRRTPSLAACAPGRPPARDGTGSMSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQFLCDEGAGISGDYIDRV
DEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGAS
PTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVD
DAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGL
KKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNI

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:65004523/chr12:58109543)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneOS9chr12:65004523chr12:58109543ENST00000257966414414_429193.0614.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000315970415414_429193.0668.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000389142414414_429193.0598.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000389146415414_429193.0653.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000435406313414_429141.0561.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000439210313414_429134.0539.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000551035313414_429160.0581.0Compositional biasNote=Asp/Glu-rich (acidic)
TgeneOS9chr12:65004523chr12:58109543ENST00000552285414414_429193.0613.0Compositional biasNote=Asp/Glu-rich (acidic)

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRASSF3chr12:65004523chr12:58109543ENST00000336061+26187_23437.0239.0DomainSARAH
HgeneRASSF3chr12:65004523chr12:58109543ENST00000336061+2679_18637.0239.0DomainRas-associating
HgeneRASSF3chr12:65004523chr12:58109543ENST00000542104+15187_23437.0239.0DomainSARAH
HgeneRASSF3chr12:65004523chr12:58109543ENST00000542104+1579_18637.0239.0DomainRas-associating
TgeneOS9chr12:65004523chr12:58109543ENST00000257966414108_230193.0614.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000315970415108_230193.0668.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000389142414108_230193.0598.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000389146415108_230193.0653.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000435406313108_230141.0561.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000439210313108_230134.0539.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000551035313108_230160.0581.0DomainMRH
TgeneOS9chr12:65004523chr12:58109543ENST00000552285414108_230193.0613.0DomainMRH


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
RASSF3
OS9


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to RASSF3-OS9


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to RASSF3-OS9


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource