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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:RBBP8-TAF4B

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RBBP8-TAF4B
	FusionPDB ID: 72620
	FusionGDB2.0 ID: 72620
		Hgene	Tgene
	Gene symbol	RBBP8	TAF4B
	Gene ID	5932	493913
	Gene name	RB binding protein 8, endonuclease	PAPPA antisense RNA 1
	Synonyms	COM1\|CTIP\|JWDS\|RIM\|SAE2\|SCKL2	DIPAS\|NCRNA00156\|PAPPA-AS\|PAPPAAS\|PAPPAS\|TAF2C2\|TAF4B\|TAFII105
	Cytomap	18q11.2	9q33.1
	Type of gene	protein-coding	ncRNA
	Description	DNA endonuclease RBBP8CTBP-interacting proteinRBBP-8retinoblastoma binding protein 8sporulation in the absence of SPO11 protein 2 homolog	DIPLA1-antisense expressedPAPPA antisense RNA 1 (non-protein coding)PAPPA antisense RNA 1 (tail to tail)
	Modification date	20200328	20200313
	UniProtAcc	RBBP8NL,C20orf151	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000327155, ENST00000360790, ENST00000399722, ENST00000399725, ENST00000581687,	ENST00000269142, ENST00000400466, ENST00000578121,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	10 X 10 X 8=800	9 X 11 X 7=693
	# samples	11	12
	** MAII score	log2(11/800*10)=-2.86249647625006 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(12/693*10)=-2.5298209465287 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: RBBP8 [Title/Abstract] AND TAF4B [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	RBBP8(20516923)-TAF4B(23845134), # samples:3
Anticipated loss of major functional domain due to fusion event.	RBBP8-TAF4B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RBBP8-TAF4B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RBBP8-TAF4B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RBBP8-TAF4B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RBBP8-TAF4B seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across RBBP8 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TAF4B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	HNSC	TCGA-BB-7872-01A	RBBP8	chr18	20516923	-	TAF4B	chr18	23845134	+
ChimerDB4	HNSC	TCGA-BB-7872-01A	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+
ChimerDB4	HNSC	TCGA-BB-7872	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+
ChimerDB4	STAD	TCGA-BR-8286	RBBP8	chr18	20581692	+	TAF4B	chr18	23865845	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000327155	RBBP8	chr18	20516923	+	ENST00000269142	TAF4B	chr18	23845134	+	4376	457	348	2702	784
ENST00000327155	RBBP8	chr18	20516923	+	ENST00000400466	TAF4B	chr18	23845134	+	1971	457	348	1970	540
ENST00000327155	RBBP8	chr18	20516923	+	ENST00000578121	TAF4B	chr18	23845134	+	2870	457	348	2717	789
ENST00000399722	RBBP8	chr18	20516923	+	ENST00000269142	TAF4B	chr18	23845134	+	4379	460	351	2705	784
ENST00000399722	RBBP8	chr18	20516923	+	ENST00000400466	TAF4B	chr18	23845134	+	1974	460	351	1973	540
ENST00000399722	RBBP8	chr18	20516923	+	ENST00000578121	TAF4B	chr18	23845134	+	2873	460	351	2720	789
ENST00000399725	RBBP8	chr18	20516923	+	ENST00000269142	TAF4B	chr18	23845134	+	4445	526	417	2771	784
ENST00000399725	RBBP8	chr18	20516923	+	ENST00000400466	TAF4B	chr18	23845134	+	2040	526	417	2039	540
ENST00000399725	RBBP8	chr18	20516923	+	ENST00000578121	TAF4B	chr18	23845134	+	2939	526	417	2786	789
ENST00000360790	RBBP8	chr18	20516923	+	ENST00000269142	TAF4B	chr18	23845134	+	4151	232	123	2477	784
ENST00000360790	RBBP8	chr18	20516923	+	ENST00000400466	TAF4B	chr18	23845134	+	1746	232	123	1745	540
ENST00000360790	RBBP8	chr18	20516923	+	ENST00000578121	TAF4B	chr18	23845134	+	2645	232	123	2492	789

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000327155	ENST00000269142	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.000879189	0.9991208
ENST00000327155	ENST00000400466	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.002525712	0.99747425
ENST00000327155	ENST00000578121	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.002385918	0.99761415
ENST00000399722	ENST00000269142	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.000827352	0.9991726
ENST00000399722	ENST00000400466	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.00214309	0.9978569
ENST00000399722	ENST00000578121	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.002446336	0.99755365
ENST00000399725	ENST00000269142	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.000789172	0.99921083
ENST00000399725	ENST00000400466	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.001846652	0.9981534
ENST00000399725	ENST00000578121	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.002306915	0.997693
ENST00000360790	ENST00000269142	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.000985138	0.99901485
ENST00000360790	ENST00000400466	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.003021152	0.9969789
ENST00000360790	ENST00000578121	RBBP8	chr18	20516923	+	TAF4B	chr18	23845134	+	0.003195238	0.9968047

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>72620_72620_1_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000327155_TAF4B_chr18_23845134_ENST00000269142_length(amino acids)=784AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDIND
VTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIAQHRMT
TYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRK

--------------------------------------------------------------

>72620_72620_2_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000327155_TAF4B_chr18_23845134_ENST00000400466_length(amino acids)=540AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRSTFICVY

--------------------------------------------------------------

>72620_72620_3_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000327155_TAF4B_chr18_23845134_ENST00000578121_length(amino acids)=789AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLFSLFQVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDE
DDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIA
QHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAA

--------------------------------------------------------------

>72620_72620_4_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000360790_TAF4B_chr18_23845134_ENST00000269142_length(amino acids)=784AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDIND
VTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIAQHRMT
TYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRK

--------------------------------------------------------------

>72620_72620_5_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000360790_TAF4B_chr18_23845134_ENST00000400466_length(amino acids)=540AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRSTFICVY

--------------------------------------------------------------

>72620_72620_6_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000360790_TAF4B_chr18_23845134_ENST00000578121_length(amino acids)=789AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLFSLFQVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDE
DDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIA
QHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAA

--------------------------------------------------------------

>72620_72620_7_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000399722_TAF4B_chr18_23845134_ENST00000269142_length(amino acids)=784AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDIND
VTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIAQHRMT
TYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRK

--------------------------------------------------------------

>72620_72620_8_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000399722_TAF4B_chr18_23845134_ENST00000400466_length(amino acids)=540AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRSTFICVY

--------------------------------------------------------------

>72620_72620_9_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000399722_TAF4B_chr18_23845134_ENST00000578121_length(amino acids)=789AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLFSLFQVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDE
DDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIA
QHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAA

--------------------------------------------------------------

>72620_72620_10_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000399725_TAF4B_chr18_23845134_ENST00000269142_length(amino acids)=784AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDIND
VTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIAQHRMT
TYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRK

--------------------------------------------------------------

>72620_72620_11_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000399725_TAF4B_chr18_23845134_ENST00000400466_length(amino acids)=540AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRSTFICVY

--------------------------------------------------------------

>72620_72620_12_RBBP8-TAF4B_RBBP8_chr18_20516923_ENST00000399725_TAF4B_chr18_23845134_ENST00000578121_length(amino acids)=789AA_BP=36
MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ
LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN
VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT
SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV
TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK
QLFSLFQVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDE
DDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIA
QHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAA

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr18:20516923/chr18:23845134)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
RBBP8 RBBP8NL,C20orf151	.
664	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000269142

722_787

114.33333333333333

863.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000400466

722_787

114.33333333333333

619.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000269142

256_353

114.33333333333333

863.0

Domain

TAFH

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000269142

653_702

114.33333333333333

863.0

Domain

Note=Histone-fold

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000400466

256_353

114.33333333333333

619.0

Domain

TAFH

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000400466

653_702

114.33333333333333

619.0

Domain

Note=Histone-fold

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000269142

516_556

114.33333333333333

863.0

Motif

Note=Nuclear export signal

Tgene

TAF4B

chr18:20516923

chr18:23845134

ENST00000400466

516_556

114.33333333333333

619.0

Motif

Note=Nuclear export signal

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000327155

28_157

36.333333333333336

898.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399722

28_157

36.333333333333336

898.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399725

28_157

36.333333333333336

868.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000327155

490_494

36.333333333333336

898.0

Motif

Note=PXDLS motif

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000327155

840_842

36.333333333333336

898.0

Motif

KLHL15-binding

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399722

490_494

36.333333333333336

898.0

Motif

Note=PXDLS motif

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399722

840_842

36.333333333333336

898.0

Motif

KLHL15-binding

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399725

490_494

36.333333333333336

868.0

Motif

Note=PXDLS motif

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399725

840_842

36.333333333333336

868.0

Motif

KLHL15-binding

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000327155

22_45

36.333333333333336

898.0

Region

Note=Essential for binding to the MRN complex and for RPA focus formation on DNA damage

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000327155

509_557

36.333333333333336

898.0

Region

Note=Damage-recruitment motif

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399722

22_45

36.333333333333336

898.0

Region

Note=Essential for binding to the MRN complex and for RPA focus formation on DNA damage

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399722

509_557

36.333333333333336

898.0

Region

Note=Damage-recruitment motif

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399725

22_45

36.333333333333336

868.0

Region

Note=Essential for binding to the MRN complex and for RPA focus formation on DNA damage

Hgene

RBBP8

chr18:20516923

chr18:23845134

ENST00000399725

509_557

36.333333333333336

868.0

Region

Note=Damage-recruitment motif

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1572_RBBP8_20516923_TAF4B_23845134_ranked_0.pdb	RBBP8	20516923	20516923	ENST00000578121	TAF4B	chr18	23845134	+	MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQ LIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLEN VKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQT SSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLV TSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVK QLFSLFQVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDE DDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIA QHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAA	789

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

RBBP8_pLDDT.png

TAF4B_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
RBBP8
TAF4B

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to RBBP8-TAF4B

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to RBBP8-TAF4B

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source