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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:REV3L-PPA2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: REV3L-PPA2
	FusionPDB ID: 73593
	FusionGDB2.0 ID: 73593
		Hgene	Tgene
	Gene symbol	REV3L	PPA2
	Gene ID	5980	27068
	Gene name	REV3 like, DNA directed polymerase zeta catalytic subunit	inorganic pyrophosphatase 2
	Synonyms	POLZ\|REV3	HSPC124\|SCFAI\|SCFI\|SID6-306
	Cytomap	6q21	4q24
	Type of gene	protein-coding	protein-coding
	Description	DNA polymerase zeta catalytic subunitREV3-like, polymerase (DNA directed), zeta, catalytic subunitRev-3, yeast, homolog-like (polymerase, DNA, zeta)	inorganic pyrophosphatase 2, mitochondrialPPase 2pyrophosphatase (inorganic) 2pyrophosphatase SID6-306pyrophosphate phospho-hydrolase 2
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000358835, ENST00000368802, ENST00000368805, ENST00000435970, ENST00000462119,	ENST00000509426, ENST00000310267, ENST00000341695, ENST00000348706, ENST00000354147, ENST00000357415, ENST00000380004, ENST00000432483,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	17 X 20 X 10=3400	17 X 10 X 8=1360
	# samples	26	18
	** MAII score	log2(26/3400*10)=-3.70895121799661 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(18/1360*10)=-2.91753783980803 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: REV3L [Title/Abstract] AND PPA2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	REV3L(111710309)-PPA2(106320323), # samples:2
Anticipated loss of major functional domain due to fusion event.	REV3L-PPA2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. REV3L-PPA2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. REV3L-PPA2 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across REV3L (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across PPA2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChiTaRS5.0	N/A	DB572905	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-
ChiTaRS5.0	N/A	DB636770	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000368802	REV3L	chr6	111710309	-	ENST00000341695	PPA2	chr4	106320323	-	2183	1185	323	1534	403
ENST00000368802	REV3L	chr6	111710309	-	ENST00000348706	PPA2	chr4	106320323	-	2017	1185	323	1534	403
ENST00000368802	REV3L	chr6	111710309	-	ENST00000432483	PPA2	chr4	106320323	-	1695	1185	323	1534	403
ENST00000368802	REV3L	chr6	111710309	-	ENST00000354147	PPA2	chr4	106320323	-	1695	1185	323	1534	403
ENST00000368802	REV3L	chr6	111710309	-	ENST00000380004	PPA2	chr4	106320323	-	1692	1185	323	1534	403
ENST00000368802	REV3L	chr6	111710309	-	ENST00000357415	PPA2	chr4	106320323	-	1692	1185	323	1534	403
ENST00000368805	REV3L	chr6	111710309	-	ENST00000341695	PPA2	chr4	106320323	-	1943	945	83	1294	403
ENST00000368805	REV3L	chr6	111710309	-	ENST00000348706	PPA2	chr4	106320323	-	1777	945	83	1294	403
ENST00000368805	REV3L	chr6	111710309	-	ENST00000432483	PPA2	chr4	106320323	-	1455	945	83	1294	403
ENST00000368805	REV3L	chr6	111710309	-	ENST00000354147	PPA2	chr4	106320323	-	1455	945	83	1294	403
ENST00000368805	REV3L	chr6	111710309	-	ENST00000380004	PPA2	chr4	106320323	-	1452	945	83	1294	403
ENST00000368805	REV3L	chr6	111710309	-	ENST00000357415	PPA2	chr4	106320323	-	1452	945	83	1294	403
ENST00000358835	REV3L	chr6	111710309	-	ENST00000341695	PPA2	chr4	106320323	-	2315	1317	455	1666	403
ENST00000358835	REV3L	chr6	111710309	-	ENST00000348706	PPA2	chr4	106320323	-	2149	1317	455	1666	403
ENST00000358835	REV3L	chr6	111710309	-	ENST00000432483	PPA2	chr4	106320323	-	1827	1317	455	1666	403
ENST00000358835	REV3L	chr6	111710309	-	ENST00000354147	PPA2	chr4	106320323	-	1827	1317	455	1666	403
ENST00000358835	REV3L	chr6	111710309	-	ENST00000380004	PPA2	chr4	106320323	-	1824	1317	455	1666	403
ENST00000358835	REV3L	chr6	111710309	-	ENST00000357415	PPA2	chr4	106320323	-	1824	1317	455	1666	403

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000368802	ENST00000341695	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.00262239	0.99737763
ENST00000368802	ENST00000348706	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.003701964	0.996298
ENST00000368802	ENST00000432483	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002467643	0.99753237
ENST00000368802	ENST00000354147	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002467643	0.99753237
ENST00000368802	ENST00000380004	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.00255658	0.99744344
ENST00000368802	ENST00000357415	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.00255658	0.99744344
ENST00000368805	ENST00000341695	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.00183775	0.9981622
ENST00000368805	ENST00000348706	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002446018	0.997554
ENST00000368805	ENST00000432483	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.001431248	0.9985688
ENST00000368805	ENST00000354147	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.001431248	0.9985688
ENST00000368805	ENST00000380004	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.001496152	0.9985039
ENST00000368805	ENST00000357415	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.001496152	0.9985039
ENST00000358835	ENST00000341695	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002680174	0.9973199
ENST00000358835	ENST00000348706	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.003646711	0.99635327
ENST00000358835	ENST00000432483	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002482509	0.99751747
ENST00000358835	ENST00000354147	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002482509	0.99751747
ENST00000358835	ENST00000380004	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002570384	0.99742955
ENST00000358835	ENST00000357415	REV3L	chr6	111710309	-	PPA2	chr4	106320323	-	0.002570384	0.99742955

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>73593_73593_1_REV3L-PPA2_REV3L_chr6_111710309_ENST00000358835_PPA2_chr4_106320323_ENST00000341695_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_2_REV3L-PPA2_REV3L_chr6_111710309_ENST00000358835_PPA2_chr4_106320323_ENST00000348706_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_3_REV3L-PPA2_REV3L_chr6_111710309_ENST00000358835_PPA2_chr4_106320323_ENST00000354147_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_4_REV3L-PPA2_REV3L_chr6_111710309_ENST00000358835_PPA2_chr4_106320323_ENST00000357415_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_5_REV3L-PPA2_REV3L_chr6_111710309_ENST00000358835_PPA2_chr4_106320323_ENST00000380004_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_6_REV3L-PPA2_REV3L_chr6_111710309_ENST00000358835_PPA2_chr4_106320323_ENST00000432483_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_7_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368802_PPA2_chr4_106320323_ENST00000341695_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_8_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368802_PPA2_chr4_106320323_ENST00000348706_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_9_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368802_PPA2_chr4_106320323_ENST00000354147_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_10_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368802_PPA2_chr4_106320323_ENST00000357415_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_11_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368802_PPA2_chr4_106320323_ENST00000380004_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_12_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368802_PPA2_chr4_106320323_ENST00000432483_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_13_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368805_PPA2_chr4_106320323_ENST00000341695_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_14_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368805_PPA2_chr4_106320323_ENST00000348706_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_15_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368805_PPA2_chr4_106320323_ENST00000354147_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

--------------------------------------------------------------

>73593_73593_16_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368805_PPA2_chr4_106320323_ENST00000357415_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

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>73593_73593_17_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368805_PPA2_chr4_106320323_ENST00000380004_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

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>73593_73593_18_REV3L-PPA2_REV3L_chr6_111710309_ENST00000368805_PPA2_chr4_106320323_ENST00000432483_length(amino acids)=403AA_BP=287
MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA
LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV
KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ
RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:111710309/chr4:106320323)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000358835

3086_3104

287.3333333333333

3131.0

Motif

Note=CysB motif

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000368802

3086_3104

287.3333333333333

3131.0

Motif

Note=CysB motif

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000368805

3086_3104

287.3333333333333

3131.0

Motif

Note=CysB motif

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000435970

3086_3104

209.33333333333334

3053.0

Motif

Note=CysB motif

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000358835

3042_3057

287.3333333333333

3131.0

Zinc finger

Note=CysA-type

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000368802

3042_3057

287.3333333333333

3131.0

Zinc finger

Note=CysA-type

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000368805

3042_3057

287.3333333333333

3131.0

Zinc finger

Note=CysA-type

Hgene

REV3L

chr6:111710309

chr4:106320323

ENST00000435970

3042_3057

209.33333333333334

3053.0

Zinc finger

Note=CysA-type

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>810_REV3L_111710309_PPA2_106320323_ranked_0.pdb	REV3L	111710309	111710309	ENST00000357415	PPA2	chr4	106320323	-	MFSVRIVTADYYMASPLQGLDTCQSPLTQAPVKKVPVVRVFGATPAGQKTCLHLHGIFPYLYVPYDGYGQQPESYLSQMAFSIDRALNVA LGNPSSTAQHVFKVSLVSGMPFYGYHEKERHFMKIYLYNPTMVKRICELLQSGAIMNKFYQPHEAHIPYLLQLFIDYNLYGMNLINLAAV KFRKARRKSNTLHATGSCKNHLSGNSLADTLFRWEQDEIPSSLILEGVEPQSTCELEVDAVAADILNRLDIEAQIGGNPGLQAIWEDEKQ RRRNRNETSQMSQPESQDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCT	403

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

REV3L_pLDDT.png

PPA2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
REV3L
PPA2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to REV3L-PPA2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to REV3L-PPA2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source