UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level1

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:RFWD2-AKT3

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RFWD2-AKT3
	FusionPDB ID: 73668
	FusionGDB2.0 ID: 73668
		Hgene	Tgene
	Gene symbol	RFWD2	AKT3
	Gene ID	64326	10000
	Gene name	COP1 E3 ubiquitin ligase	AKT serine/threonine kinase 3
	Synonyms	CFAP78\|FAP78\|RFWD2\|RNF200	MPPH\|MPPH2\|PKB-GAMMA\|PKBG\|PRKBG\|RAC-PK-gamma\|RAC-gamma\|STK-2
	Cytomap	1q25.1-q25.2	1q43-q44
	Type of gene	protein-coding	protein-coding
	Description	E3 ubiquitin-protein ligase COP1E3 ubiquitin-protein ligase RFWD2RING finger protein 200RING-type E3 ubiquitin transferase RFWD2constitutive photomorphogenesis protein 1 homologconstitutive photomorphogenic protein 1putative ubiquitin ligase COP1ri	RAC-gamma serine/threonine-protein kinasePKB gammaRAC-gamma serine/threonine protein kinasev-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
	Modification date	20200313	20200313
	UniProtAcc	.	Q9Y243
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000308769, ENST00000367669,	ENST00000492957, ENST00000263826, ENST00000336199, ENST00000366539, ENST00000366540,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	17 X 15 X 10=2550	19 X 18 X 8=2736
	# samples	20	19
	** MAII score	log2(20/2550*10)=-3.6724253419715 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(19/2736*10)=-3.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: RFWD2 [Title/Abstract] AND AKT3 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	RFWD2(175956078)-AKT3(243727150), # samples:1
Anticipated loss of major functional domain due to fusion event.	RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	RFWD2	GO:0010212	response to ionizing radiation	19805145
Tgene	AKT3	GO:0043536	positive regulation of blood vessel endothelial cell migration	28254819
Tgene	AKT3	GO:1905564	positive regulation of vascular endothelial cell proliferation	28254819

Fusion gene breakpoints across RFWD2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across AKT3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-D7-A4Z0	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000367669	RFWD2	chr1	175956078	-	ENST00000336199	AKT3	chr1	243727150	-	3451	2648	515	3226	903
ENST00000367669	RFWD2	chr1	175956078	-	ENST00000366540	AKT3	chr1	243727150	-	3251	2648	515	3226	903
ENST00000367669	RFWD2	chr1	175956078	-	ENST00000366539	AKT3	chr1	243727150	-	6755	2648	515	3268	917
ENST00000367669	RFWD2	chr1	175956078	-	ENST00000263826	AKT3	chr1	243727150	-	5336	2648	515	3268	917
ENST00000308769	RFWD2	chr1	175956078	-	ENST00000336199	AKT3	chr1	243727150	-	2864	2061	0	2639	879
ENST00000308769	RFWD2	chr1	175956078	-	ENST00000366540	AKT3	chr1	243727150	-	2664	2061	0	2639	879
ENST00000308769	RFWD2	chr1	175956078	-	ENST00000366539	AKT3	chr1	243727150	-	6168	2061	0	2681	893
ENST00000308769	RFWD2	chr1	175956078	-	ENST00000263826	AKT3	chr1	243727150	-	4749	2061	0	2681	893

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000367669	ENST00000336199	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000699014	0.99930096
ENST00000367669	ENST00000366540	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000643414	0.99935657
ENST00000367669	ENST00000366539	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000432505	0.99956745
ENST00000367669	ENST00000263826	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000627856	0.9993722
ENST00000308769	ENST00000336199	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000701318	0.9992987
ENST00000308769	ENST00000366540	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000656195	0.9993438
ENST00000308769	ENST00000366539	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000427827	0.9995722
ENST00000308769	ENST00000263826	RFWD2	chr1	175956078	-	AKT3	chr1	243727150	-	0.000614566	0.99938536

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>73668_73668_1_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000263826_length(amino acids)=893AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------

>73668_73668_2_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000336199_length(amino acids)=879AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------

>73668_73668_3_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000366539_length(amino acids)=893AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------

>73668_73668_4_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000366540_length(amino acids)=879AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------

>73668_73668_5_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000263826_length(amino acids)=917AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDN

--------------------------------------------------------------

>73668_73668_6_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000336199_length(amino acids)=903AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKCQQSDCGMLGN

--------------------------------------------------------------

>73668_73668_7_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000366539_length(amino acids)=917AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDN

--------------------------------------------------------------

>73668_73668_8_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000366540_length(amino acids)=903AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKCQQSDCGMLGN

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:175956078/chr1:243727150)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	AKT3 Q9Y243
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. {ECO:0000269\|PubMed:18524868, ECO:0000269\|PubMed:21191416}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

233_301

687.0

708.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

233_301

711.0

732.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

2_110

687.0

708.0

Compositional bias

Note=Ser-rich

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

2_110

711.0

732.0

Compositional bias

Note=Ser-rich

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

109_113

687.0

708.0

Motif

Note=Nuclear localization signal 1

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

195_206

687.0

708.0

Motif

Note=Nuclear localization signal 2

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

235_245

687.0

708.0

Motif

Nuclear export signal

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

109_113

711.0

732.0

Motif

Note=Nuclear localization signal 1

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

195_206

711.0

732.0

Motif

Note=Nuclear localization signal 2

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

235_245

711.0

732.0

Motif

Nuclear export signal

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

419_458

687.0

708.0

Repeat

Note=WD 1

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

468_508

687.0

708.0

Repeat

Note=WD 2

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

511_551

687.0

708.0

Repeat

Note=WD 3

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

553_593

687.0

708.0

Repeat

Note=WD 4

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

597_635

687.0

708.0

Repeat

Note=WD 5

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

638_677

687.0

708.0

Repeat

Note=WD 6

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

419_458

711.0

732.0

Repeat

Note=WD 1

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

468_508

711.0

732.0

Repeat

Note=WD 2

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

511_551

711.0

732.0

Repeat

Note=WD 3

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

553_593

711.0

732.0

Repeat

Note=WD 4

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

597_635

711.0

732.0

Repeat

Note=WD 5

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

638_677

711.0

732.0

Repeat

Note=WD 6

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

136_174

687.0

708.0

Zinc finger

RING-type

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

136_174

711.0

732.0

Zinc finger

RING-type

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000263826

406_479

273.0

480.0

Domain

AGC-kinase C-terminal

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000336199

406_479

273.0

476.0

Domain

AGC-kinase C-terminal

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366539

406_479

273.0

480.0

Domain

AGC-kinase C-terminal

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366540

406_479

273.0

466.0

Domain

AGC-kinase C-terminal

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000308769

691_729

687.0

708.0

Repeat

Note=WD 7

Hgene

RFWD2

chr1:175956078

chr1:243727150

ENST00000367669

691_729

711.0

732.0

Repeat

Note=WD 7

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000263826

148_405

273.0

480.0

Domain

Protein kinase

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000263826

5_107

273.0

480.0

Domain

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000336199

148_405

273.0

476.0

Domain

Protein kinase

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000336199

5_107

273.0

476.0

Domain

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366539

148_405

273.0

480.0

Domain

Protein kinase

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366539

5_107

273.0

480.0

Domain

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366540

148_405

273.0

466.0

Domain

Protein kinase

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366540

5_107

273.0

466.0

Domain

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000263826

154_162

273.0

480.0

Nucleotide binding

ATP

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000336199

154_162

273.0

476.0

Nucleotide binding

ATP

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366539

154_162

273.0

480.0

Nucleotide binding

ATP

Tgene

AKT3

chr1:175956078

chr1:243727150

ENST00000366540

154_162

273.0

466.0

Nucleotide binding

ATP

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
RFWD2
AKT3

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to RFWD2-AKT3

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to RFWD2-AKT3

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source