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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ATF7IP-TMPRSS2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ATF7IP-TMPRSS2
	FusionPDB ID: 7491
	FusionGDB2.0 ID: 7491
		Hgene	Tgene
	Gene symbol	ATF7IP	TMPRSS2
	Gene ID	55729	7113
	Gene name	activating transcription factor 7 interacting protein	transmembrane serine protease 2
	Synonyms	AM\|ATF-IP\|MCAF\|MCAF1\|p621	PP9284\|PRSS10
	Cytomap	12p13.1	21q22.3
	Type of gene	protein-coding	protein-coding
	Description	activating transcription factor 7-interacting protein 1ATF-interacting proteinATF7-interacting proteinATFa-associated modulatorMBD1-containing chromatin-associated factor 1	transmembrane protease serine 2epitheliasinserine protease 10transmembrane protease, serine 2
	Modification date	20200313	20200313
	UniProtAcc	Q5U623	O15393
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000261168, ENST00000536444, ENST00000540793, ENST00000543189, ENST00000544627, ENST00000541654,	ENST00000497881, ENST00000332149, ENST00000398585, ENST00000458356,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	16 X 15 X 8=1920	28 X 34 X 5=4760
	# samples	20	35
	** MAII score	log2(20/1920*10)=-3.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(35/4760*10)=-3.76553474636298 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ATF7IP [Title/Abstract] AND TMPRSS2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ATF7IP(14610229)-TMPRSS2(42861520), # samples:4 TMPRSS2(42870045)-ATF7IP(14613428), # samples:3
Anticipated loss of major functional domain due to fusion event.	ATF7IP-TMPRSS2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ATF7IP-TMPRSS2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ATF7IP-TMPRSS2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ATF7IP-TMPRSS2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. TMPRSS2-ATF7IP seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. TMPRSS2-ATF7IP seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. TMPRSS2-ATF7IP seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. TMPRSS2-ATF7IP seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ATF7IP	GO:0006306	DNA methylation	12665582
Hgene	ATF7IP	GO:0045892	negative regulation of transcription, DNA-templated	12665582
Hgene	ATF7IP	GO:0045893	positive regulation of transcription, DNA-templated	12665582
Hgene	ATF7IP	GO:0045898	regulation of RNA polymerase II transcriptional preinitiation complex assembly	12665582
Tgene	TMPRSS2	GO:0006508	proteolysis	21068237\|24227843
Tgene	TMPRSS2	GO:0046598	positive regulation of viral entry into host cell	21068237\|24227843

Fusion gene breakpoints across ATF7IP (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TMPRSS2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-EJ-7784-01A	ATF7IP	chr12	14610229	-	TMPRSS2	chr21	42861520	-
ChimerDB4	PRAD	TCGA-EJ-7784-01A	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-
ChimerDB4	PRAD	TCGA-EJ-7784	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000261168	ATF7IP	chr12	14610229	+	ENST00000332149	TMPRSS2	chr21	42861520	-	5143	2311	153	3551	1132
ENST00000261168	ATF7IP	chr12	14610229	+	ENST00000398585	TMPRSS2	chr21	42861520	-	5141	2311	153	3551	1132
ENST00000261168	ATF7IP	chr12	14610229	+	ENST00000458356	TMPRSS2	chr21	42861520	-	3685	2311	153	3551	1132
ENST00000543189	ATF7IP	chr12	14610229	+	ENST00000332149	TMPRSS2	chr21	42861520	-	5129	2297	142	3537	1131
ENST00000543189	ATF7IP	chr12	14610229	+	ENST00000398585	TMPRSS2	chr21	42861520	-	5127	2297	142	3537	1131
ENST00000543189	ATF7IP	chr12	14610229	+	ENST00000458356	TMPRSS2	chr21	42861520	-	3671	2297	142	3537	1131
ENST00000536444	ATF7IP	chr12	14610229	+	ENST00000332149	TMPRSS2	chr21	42861520	-	5093	2261	106	3501	1131
ENST00000536444	ATF7IP	chr12	14610229	+	ENST00000398585	TMPRSS2	chr21	42861520	-	5091	2261	106	3501	1131
ENST00000536444	ATF7IP	chr12	14610229	+	ENST00000458356	TMPRSS2	chr21	42861520	-	3635	2261	106	3501	1131
ENST00000544627	ATF7IP	chr12	14610229	+	ENST00000332149	TMPRSS2	chr21	42861520	-	5334	2502	320	3742	1140
ENST00000544627	ATF7IP	chr12	14610229	+	ENST00000398585	TMPRSS2	chr21	42861520	-	5332	2502	320	3742	1140
ENST00000544627	ATF7IP	chr12	14610229	+	ENST00000458356	TMPRSS2	chr21	42861520	-	3876	2502	320	3742	1140
ENST00000540793	ATF7IP	chr12	14610229	+	ENST00000332149	TMPRSS2	chr21	42861520	-	5145	2313	155	3553	1132
ENST00000540793	ATF7IP	chr12	14610229	+	ENST00000398585	TMPRSS2	chr21	42861520	-	5143	2313	155	3553	1132
ENST00000540793	ATF7IP	chr12	14610229	+	ENST00000458356	TMPRSS2	chr21	42861520	-	3687	2313	155	3553	1132

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000261168	ENST00000332149	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000355261	0.99964476
ENST00000261168	ENST00000398585	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000358682	0.9996413
ENST00000261168	ENST00000458356	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000425301	0.9995747
ENST00000543189	ENST00000332149	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000280247	0.9997197
ENST00000543189	ENST00000398585	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000283135	0.9997168
ENST00000543189	ENST00000458356	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000326919	0.9996731
ENST00000536444	ENST00000332149	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000253249	0.99974674
ENST00000536444	ENST00000398585	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000255738	0.99974424
ENST00000536444	ENST00000458356	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.00027842	0.9997216
ENST00000544627	ENST00000332149	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000365156	0.99963486
ENST00000544627	ENST00000398585	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000368775	0.99963117
ENST00000544627	ENST00000458356	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000392033	0.999608
ENST00000540793	ENST00000332149	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000290283	0.99970967
ENST00000540793	ENST00000398585	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000293098	0.9997069
ENST00000540793	ENST00000458356	ATF7IP	chr12	14610229	+	TMPRSS2	chr21	42861520	-	0.000303438	0.9996966

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>7491_7491_1_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000261168_TMPRSS2_chr21_42861520_ENST00000332149_length(amino acids)=1132AA_BP=719
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPEEEEQVIHE
DDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIE
CNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTE
TKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHP
VCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGE
SALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMK
LQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGD

--------------------------------------------------------------

>7491_7491_2_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000261168_TMPRSS2_chr21_42861520_ENST00000398585_length(amino acids)=1132AA_BP=719
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPEEEEQVIHE
DDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIE
CNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTE
TKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHP
VCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGE
SALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMK
LQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGD

--------------------------------------------------------------

>7491_7491_3_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000261168_TMPRSS2_chr21_42861520_ENST00000458356_length(amino acids)=1132AA_BP=719
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPEEEEQVIHE
DDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIE
CNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTE
TKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHP
VCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGE
SALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMK
LQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGD

--------------------------------------------------------------

>7491_7491_4_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000536444_TMPRSS2_chr21_42861520_ENST00000332149_length(amino acids)=1131AA_BP=718
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHED
DERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC
NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTET
KKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPV
CQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGES
ALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKL
QKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDS

--------------------------------------------------------------

>7491_7491_5_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000536444_TMPRSS2_chr21_42861520_ENST00000398585_length(amino acids)=1131AA_BP=718
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHED
DERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC
NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTET
KKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPV
CQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGES
ALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKL
QKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDS

--------------------------------------------------------------

>7491_7491_6_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000536444_TMPRSS2_chr21_42861520_ENST00000458356_length(amino acids)=1131AA_BP=718
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHED
DERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC
NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTET
KKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPV
CQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGES
ALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKL
QKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDS

--------------------------------------------------------------

>7491_7491_7_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000540793_TMPRSS2_chr21_42861520_ENST00000332149_length(amino acids)=1132AA_BP=719
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPEEEEQVIHE
DDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIE
CNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTE
TKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHP
VCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGE
SALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMK
LQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGD

--------------------------------------------------------------

>7491_7491_8_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000540793_TMPRSS2_chr21_42861520_ENST00000398585_length(amino acids)=1132AA_BP=719
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPEEEEQVIHE
DDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIE
CNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTE
TKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHP
VCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGE
SALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMK
LQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGD

--------------------------------------------------------------

>7491_7491_9_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000540793_TMPRSS2_chr21_42861520_ENST00000458356_length(amino acids)=1132AA_BP=719
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPEEEEQVIHE
DDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIE
CNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTE
TKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHP
VCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGE
SALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMK
LQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGD

--------------------------------------------------------------

>7491_7491_10_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000543189_TMPRSS2_chr21_42861520_ENST00000332149_length(amino acids)=1131AA_BP=718
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHED
DERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC
NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTET
KKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPV
CQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGES
ALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKL
QKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDS

--------------------------------------------------------------

>7491_7491_11_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000543189_TMPRSS2_chr21_42861520_ENST00000398585_length(amino acids)=1131AA_BP=718
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHED
DERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC
NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTET
KKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPV
CQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGES
ALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKL
QKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDS

--------------------------------------------------------------

>7491_7491_12_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000543189_TMPRSS2_chr21_42861520_ENST00000458356_length(amino acids)=1131AA_BP=718
MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPEGSKAEWKE
TPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAP
SGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLA
TGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETLETDDTTIC
SDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELT
CFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHED
DERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC
NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTET
KKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPV
CQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGES
ALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKL
QKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDS

--------------------------------------------------------------

>7491_7491_13_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000544627_TMPRSS2_chr21_42861520_ENST00000332149_length(amino acids)=1140AA_BP=727
MHQDQRFRMDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPE
GSKAEWKETPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASG
DATSGDAPSGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSS
ELASDDLATGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETL
ETDDTTICSDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKL
APSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPE
EEEQVIHEDDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAEL
KTRVEKIECNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPS
FQTPVNTETKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYS
SQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSR
QSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTK
NNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQG

--------------------------------------------------------------

>7491_7491_14_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000544627_TMPRSS2_chr21_42861520_ENST00000398585_length(amino acids)=1140AA_BP=727
MHQDQRFRMDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPE
GSKAEWKETPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASG
DATSGDAPSGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSS
ELASDDLATGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETL
ETDDTTICSDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKL
APSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPE
EEEQVIHEDDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAEL
KTRVEKIECNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPS
FQTPVNTETKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYS
SQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSR
QSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTK
NNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQG

--------------------------------------------------------------

>7491_7491_15_ATF7IP-TMPRSS2_ATF7IP_chr12_14610229_ENST00000544627_TMPRSS2_chr21_42861520_ENST00000458356_length(amino acids)=1140AA_BP=727
MHQDQRFRMDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPE
GSKAEWKETPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASG
DATSGDAPSGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSS
ELASDDLATGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETL
ETDDTTICSDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKL
APSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPE
EEEQVIHEDDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAEL
KTRVEKIECNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPS
FQTPVNTETKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYS
SQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSR
QSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTK
NNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQG

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:14610229/chr21:42861520)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ATF7IP Q5U623	TMPRSS2 O15393
FUNCTION: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (Probable). {ECO:0000305}.	FUNCTION: Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed:25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed:15537383, PubMed:26018085, PubMed:25122198). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity). {ECO:0000250\|UniProtKB:Q9JIQ8, ECO:0000269\|PubMed:15537383, ECO:0000269\|PubMed:25122198, ECO:0000269\|PubMed:26018085}.; FUNCTION: (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry (PubMed:24227843, PubMed:32142651, PubMed:32404436, PubMed:34159616, PubMed:33051876). Upon SARS-CoV-2 infection, increases syncytia formation by accelerating the fusion process (PubMed:34159616, PubMed:33051876). Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity. {ECO:0000269\|PubMed:21068237, ECO:0000269\|PubMed:21325420, ECO:0000269\|PubMed:23536651, ECO:0000269\|PubMed:23966399, ECO:0000269\|PubMed:24027332, ECO:0000269\|PubMed:24227843, ECO:0000269\|PubMed:32142651, ECO:0000269\|PubMed:32404436, ECO:0000269\|PubMed:33051876, ECO:0000269\|PubMed:34159616}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000261168

617_665

719.3333333333334

1271.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000540793

617_665

719.3333333333334

1271.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000543189

617_665

718.3333333333334

1106.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000261168

349_580

719.3333333333334

1271.0

Compositional bias

Note=Glu-rich

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000540793

349_580

719.3333333333334

1271.0

Compositional bias

Note=Glu-rich

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000543189

349_580

718.3333333333334

1106.0

Compositional bias

Note=Glu-rich

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000261168

553_571

719.3333333333334

1271.0

Motif

Nuclear localization signal

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000540793

553_571

719.3333333333334

1271.0

Motif

Nuclear localization signal

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000543189

553_571

718.3333333333334

1106.0

Motif

Nuclear localization signal

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000332149

112_149

79.33333333333333

493.0

Domain

LDL-receptor class A

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000332149

150_242

79.33333333333333

493.0

Domain

SRCR

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000332149

256_489

79.33333333333333

493.0

Domain

Peptidase S1

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000398585

150_242

116.33333333333333

530.0

Domain

SRCR

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000398585

256_489

116.33333333333333

530.0

Domain

Peptidase S1

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000458356

112_149

79.33333333333333

493.0

Domain

LDL-receptor class A

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000458356

150_242

79.33333333333333

493.0

Domain

SRCR

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000458356

256_489

79.33333333333333

493.0

Domain

Peptidase S1

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000332149

106_492

79.33333333333333

493.0

Topological domain

Extracellular

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000458356

106_492

79.33333333333333

493.0

Topological domain

Extracellular

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000332149

85_105

79.33333333333333

493.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000458356

85_105

79.33333333333333

493.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000261168

1160_1270

719.3333333333334

1271.0

Domain

Fibronectin type-III

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000540793

1160_1270

719.3333333333334

1271.0

Domain

Fibronectin type-III

Hgene

ATF7IP

chr12:14610229

chr21:42861520

ENST00000543189

1160_1270

718.3333333333334

1106.0

Domain

Fibronectin type-III

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000398585

112_149

116.33333333333333

530.0

Domain

LDL-receptor class A

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000332149

1_84

79.33333333333333

493.0

Topological domain

Cytoplasmic

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000398585

106_492

116.33333333333333

530.0

Topological domain

Extracellular

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000398585

1_84

116.33333333333333

530.0

Topological domain

Cytoplasmic

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000458356

1_84

79.33333333333333

493.0

Topological domain

Cytoplasmic

Tgene

TMPRSS2

chr12:14610229

chr21:42861520

ENST00000398585

85_105

116.33333333333333

530.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1885_ATF7IP_14610229_TMPRSS2_42861520_ranked_0.pdb	ATF7IP	14610229	14610229	ENST00000458356	TMPRSS2	chr21	42861520	-	MHQDQRFRMDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEEVNGIEEICFDPE GSKAEWKETPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLDAGDPASGVLASGDSTSGDPTSSEPSSSDAASG DATSGDAPSGDVSPGDATSGDATADDLSSGDPTSSDPIPGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSS ELASDDLATGELASDELTSESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEETL ETDDTTICSDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKL APSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSPAEVESNEKDNKPE EEEQVIHEDDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAEL KTRVEKIECNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPS FQTPVNTETKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYS SQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSR QSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTK NNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQG	1140

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ATF7IP_pLDDT.png

TMPRSS2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

TMPRSS2

CLK1, HNRNPL, DCAF4, S, ACE2, STX7, SCD, PLP2, PLLP, MOSPD3, UPK2, SELK, C3orf52, IGFBP5, SEC22A, PLP1, CLN6, TMEM128, C14orf1, CFHR5, TMEM243, TMEM60, CXCL9, BNIP2, CNIH3, PGAP2, TMEM218, TMEM86A, CMTM7, ZFPL1, BMP10, TMEM79, SLC35A1, SFTPC, VAMP5, FAXDC2, STX8, NINJ2, CLEC7A, C2CD2L, CNIH2, BNIP3, DEFB103A, DEFB103B, TMEM11, C17orf62, TMEM86B, ADIPOQ, EDDM3B, BRICD5, PTTG1IP, PTCH1, ANKRD46, TMEM222, C1QL4, TMEM120B, FAM3C, TMEM229B, PLN, CTXN3, TNF, SMIM1, AQP1, TMPRSS2, DNASE2, MYO1C, PGRMC1, NDUFA4, ADAM10, CLGN, SEC16A, SPINT2, COCH, B4GAT1, XPOT, MYO1B, LANCL1, CALU, PLXNA2, GPC4, BANF1, CPD, CLPX, STC2, SLIT2, MYO1D, ERLIN2, BAG2, TFRC, HLA-A, TP53, RPN1, RPN2, GNAI2, APP, ITGB1, INSR, GLA, GPI, ITGAV, CTSD, APRT, IGF1R, MET, GNAI3, CLTA, CALM2, HLA-C, DLAT, HSPA5, LAMC1, LAMP1, IGF2R, PDIA4, P4HA1, HSP90B1, PVR, RPA2, ATP2A2, PFKL, CDH2, MSH3, RPA1, CALR, RPA3, LONP1, TGFBR1, DDOST, HADHA, ECE1, DNM2, FXR2, PLXNA3, ATP1B3, HADHB, SEC61A1, GNB1, NOMO3, PRKDC, TFAM, SLC7A5, PFKP, KIF23, FMR1, PTK7, ADAM15, CUL2, SCARB2, WRN, DSC3, ITIH4, GANAB, KARS, RCN1, SAFB, IMMT, HSD17B12, UBR4, QSOX2, RRM2B, MON2, YTHDF3, PABPN1, PLD3, PELP1, TTC13, CNNM3, IPO4, FAR1, GGH, ERLEC1, RBM33, DNAJA3, RBM14, PPP1R9B, SDF2, NEU1, GRWD1, AIF1L, NTPCR, FUCA2, EDEM3, RACGAP1, RAB18, TMEM106B, CHPF2, GNG12, DNAJB11, EGFL7, DDX20, ADAMTS1, LNPEP, ITM2B, MRPL11, HYOU1, AFG3L2, YTHDF2, SUPT16H, PDHX, IPO5, SAP18, HGS, SPTBN2, PLXNB2, ARPC3, ACTN4, TRIM13, PDCD6, MPDU1, SEC22B, ATP6AP2, STAM2, ARL6IP5, UFL1, SEC31A, STBD1, SMC2, SEC24A, SEC24B, ACSL3, CDK1, SRPR, SPTB, MTHFD1, ACTN1, ETFA, RAB6A, VDAC1, HMOX2, MYH10, DEK, ATP5C1, NAMPT, VDAC2, QARS, TUFM, SERPINH1, BCAP31, PSMD7, CLCN7, SLC25A1, YARS, EPPK1, RAB10, ABCE1, RHOA, CNBP, TPM4, SLC25A11, LMNB2, CKAP4, GOLGA3, TWF1, TRAP1, STX5, DDX39B, RAB3GAP1, PON2, PPA1, SEC23A, SEC23B, NDUFA9, ATP6V1F, INF2, ANO6, ACTBL2, MIA3, CDKAL1, KIAA0368, C19orf70, SLC25A24, HUWE1, MEGF8, KTN1, COMTD1, ANKLE2, VRK2, ZDHHC17, DNAH10, NUP93, MMGT1, TMEM199, ATPAF2, EMC1, PDZD8, NBEA, MOSPD2, PDCD6IP, SYNE2, UBXN4, CCDC47, LRRC59, C7orf55, DDRGK1, NGLY1, CDK5RAP3, CCDC115, CLCC1, OSBPL9, RBM15, CGRRF1, ESYT1, NDC1, EFHD1, OSBPL8, KIAA1715, LSG1, RAB1B, TMX1, GORASP2, SFXN1, XPO5, VEZT, JPH1, BIRC6, AAAS, TOMM22, DDX19A, SMPD4, TMOD3, EMC3, VAPA, CEP72, LARS, DPM3, DHCR7, UBA2, AASS, ATP6V1H, SLC25A13, STOML2, RAB21, MYO6, VDAC3, AUP1, SEC23IP,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ATF7IP
TMPRSS2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000261168	+	8	15	1154_1270	719.3333333333334	1271.0	MBD1
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000540793	+	7	14	1154_1270	719.3333333333334	1271.0	MBD1
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000543189	+	8	13	1154_1270	718.3333333333334	1106.0	MBD1
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000261168	+	8	15	562_817	719.3333333333334	1271.0	SETDB1
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000540793	+	7	14	562_817	719.3333333333334	1271.0	SETDB1
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000543189	+	8	13	562_817	718.3333333333334	1106.0	SETDB1
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000261168	+	8	15	965_975	719.3333333333334	1271.0	SUMO
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000540793	+	7	14	965_975	719.3333333333334	1271.0	SUMO
Hgene	ATF7IP	chr12:14610229	chr21:42861520	ENST00000543189	+	8	13	965_975	718.3333333333334	1106.0	SUMO

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Related Drugs to ATF7IP-TMPRSS2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to ATF7IP-TMPRSS2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Tgene	TMPRSS2	C0033578	Prostatic Neoplasms	4	CTD_human
Tgene	TMPRSS2	C0376358	Malignant neoplasm of prostate	4	CTD_human