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Center for Computational Systems Medicine level1
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:ROCK1-LRIG3

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ROCK1-LRIG3
FusionPDB ID: 75311
FusionGDB2.0 ID: 75311
HgeneTgene
Gene symbol

ROCK1

LRIG3

Gene ID

6093

121227

Gene nameRho associated coiled-coil containing protein kinase 1leucine rich repeats and immunoglobulin like domains 3
SynonymsP160ROCK|ROCK-ILIG3
Cytomap

18q11.1

12q14.1

Type of geneprotein-codingprotein-coding
Descriptionrho-associated protein kinase 1p160 ROCK-1renal carcinoma antigen NY-REN-35leucine-rich repeats and immunoglobulin-like domains protein 3LIG-3
Modification date2020032220200313
UniProtAcc.

Q6UXM1

Ensembl transtripts involved in fusion geneENST idsENST00000399799, ENST00000320743, 
ENST00000379141, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score24 X 16 X 12=46086 X 3 X 5=90
# samples 317
** MAII scorelog2(31/4608*10)=-3.89380059616807
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(7/90*10)=-0.362570079384708
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: ROCK1 [Title/Abstract] AND LRIG3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ROCK1(18625253)-LRIG3(59308117), # samples:3
Anticipated loss of major functional domain due to fusion event.ROCK1-LRIG3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ROCK1-LRIG3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ROCK1-LRIG3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ROCK1-LRIG3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneROCK1

GO:0006468

protein phosphorylation

23093407

HgeneROCK1

GO:0007159

leukocyte cell-cell adhesion

12082081

HgeneROCK1

GO:0018105

peptidyl-serine phosphorylation

18573880

HgeneROCK1

GO:0022614

membrane to membrane docking

12082081

HgeneROCK1

GO:0030334

regulation of cell migration

23093407

HgeneROCK1

GO:0032091

negative regulation of protein binding

18573880

HgeneROCK1

GO:0032956

regulation of actin cytoskeleton organization

25911094

HgeneROCK1

GO:0050900

leukocyte migration

12082081

HgeneROCK1

GO:0050901

leukocyte tethering or rolling

12082081

HgeneROCK1

GO:0070507

regulation of microtubule cytoskeleton organization

23093407


check buttonFusion gene breakpoints across ROCK1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across LRIG3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-A8-A091-01AROCK1chr18

18625253

-LRIG3chr12

59308117

-


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000399799ROCK1chr1818625253-ENST00000379141LRIG3chr1259308117-5078153189946541251
ENST00000399799ROCK1chr1818625253-ENST00000320743LRIG3chr1259308117-5078153189946541251

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000399799ENST00000379141ROCK1chr1818625253-LRIG3chr1259308117-0.0008451320.9991549
ENST00000399799ENST00000320743ROCK1chr1818625253-LRIG3chr1259308117-0.0008451320.9991549

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>75311_75311_1_ROCK1-LRIG3_ROCK1_chr18_18625253_ENST00000399799_LRIG3_chr12_59308117_ENST00000320743_length(amino acids)=1251AA_BP=210
MLVLVRGHCHSSSNMSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDY
EVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMS
NYDVPEKWARFYTAEVVLALDAIHSMGFIHRDLSHNRLSFIKASSMSHLQSLREVKLNNNELETIPNLGPVSANITLLSLAGNRIVEILP
EHLKEFQSLETLDLSSNNISELQTAFPALQLKYLYLNSNRVTSMEPGYFDNLANTLLVLKLNRNRISAIPPKMFKLPQLQHLELNRNKIK
NVDGLTFQGLGALKSLKMQRNGVTKLMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDL
TFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTG
LDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLLCDCQLKWLPQWVAENNFQSFVNASCAHPQLLKGRSIFAVSPDGFVCDDFPK
PQITVQPETQSAIKGSNLSFICSAASSSDSPMTFAWKKDNELLHDAEMENYAHLRAQGGEVMEYTTILRLREVEFASEGKYQCVISNHFG
SSYSVKAKLTVNMLPSFTKTPMDLTIRAGAMARLECAAVGHPAPQIAWQKDGGTDFPAARERRMHVMPEDDVFFIVDVKIEDIGVYSCTA
QNSAGSISANATLTVLETPSFLRPLLDRTVTKGETAVLQCIAGGSPPPKLNWTKDDSPLVVTERHFFAAGNQLLIIVDSDVSDAGKYTCE
MSNTLGTERGNVRLSVIPTPTCDSPQMTAPSLDDDGWATVGVVIIAVVCCVVGTSLVWVVIIYHTRRRNEDCSITNTDETNLPADIPSYL
SSQGTLADRQDGYVSSESGSHHQFVTSSGAGFFLPQHDSSGTCHIDNSSEADVEAATDLFLCPFLGSTGPMYLKGNVYGSDPFETYHTGC
SPDPRTVLMDHYEPSYIKKKECYPCSHPSEESCERSFSNISWPSHVRKLLNTSYSHNEGPGMKNLCLNKSSLDFSANPEPASVASSNSFM

--------------------------------------------------------------

>75311_75311_2_ROCK1-LRIG3_ROCK1_chr18_18625253_ENST00000399799_LRIG3_chr12_59308117_ENST00000379141_length(amino acids)=1251AA_BP=210
MLVLVRGHCHSSSNMSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDY
EVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMS
NYDVPEKWARFYTAEVVLALDAIHSMGFIHRDLSHNRLSFIKASSMSHLQSLREVKLNNNELETIPNLGPVSANITLLSLAGNRIVEILP
EHLKEFQSLETLDLSSNNISELQTAFPALQLKYLYLNSNRVTSMEPGYFDNLANTLLVLKLNRNRISAIPPKMFKLPQLQHLELNRNKIK
NVDGLTFQGLGALKSLKMQRNGVTKLMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDL
TFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTG
LDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLLCDCQLKWLPQWVAENNFQSFVNASCAHPQLLKGRSIFAVSPDGFVCDDFPK
PQITVQPETQSAIKGSNLSFICSAASSSDSPMTFAWKKDNELLHDAEMENYAHLRAQGGEVMEYTTILRLREVEFASEGKYQCVISNHFG
SSYSVKAKLTVNMLPSFTKTPMDLTIRAGAMARLECAAVGHPAPQIAWQKDGGTDFPAARERRMHVMPEDDVFFIVDVKIEDIGVYSCTA
QNSAGSISANATLTVLETPSFLRPLLDRTVTKGETAVLQCIAGGSPPPKLNWTKDDSPLVVTERHFFAAGNQLLIIVDSDVSDAGKYTCE
MSNTLGTERGNVRLSVIPTPTCDSPQMTAPSLDDDGWATVGVVIIAVVCCVVGTSLVWVVIIYHTRRRNEDCSITNTDETNLPADIPSYL
SSQGTLADRQDGYVSSESGSHHQFVTSSGAGFFLPQHDSSGTCHIDNSSEADVEAATDLFLCPFLGSTGPMYLKGNVYGSDPFETYHTGC
SPDPRTVLMDHYEPSYIKKKECYPCSHPSEESCERSFSNISWPSHVRKLLNTSYSHNEGPGMKNLCLNKSSLDFSANPEPASVASSNSFM

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr18:18625253/chr12:59308117)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.LRIG3

Q6UXM1

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: May play a role in craniofacial and inner ear morphogenesis during embryonic development. May act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1 (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-53382_90196.666666666666661355.0Nucleotide bindingATP
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019444_49578.666666666666671120.0DomainNote=LRRCT
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019499_59878.666666666666671120.0DomainNote=Ig-like C2-type 1
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019603_69278.666666666666671120.0DomainNote=Ig-like C2-type 2
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019697_78378.666666666666671120.0DomainNote=Ig-like C2-type 3
TgeneLRIG3chr18:18625253chr12:59308117ENST0000037914101938_7418.6666666666666681060.0DomainNote=LRRNT
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019444_49518.6666666666666681060.0DomainNote=LRRCT
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019499_59818.6666666666666681060.0DomainNote=Ig-like C2-type 1
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019603_69218.6666666666666681060.0DomainNote=Ig-like C2-type 2
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019697_78318.6666666666666681060.0DomainNote=Ig-like C2-type 3
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019122_14278.666666666666671120.0RepeatNote=LRR 3
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019146_16778.666666666666671120.0RepeatNote=LRR 4
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019168_18978.666666666666671120.0RepeatNote=LRR 5
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019193_21478.666666666666671120.0RepeatNote=LRR 6
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019216_23778.666666666666671120.0RepeatNote=LRR 7
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019240_26178.666666666666671120.0RepeatNote=LRR 8
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019264_28578.666666666666671120.0RepeatNote=LRR 9
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019288_30978.666666666666671120.0RepeatNote=LRR 10
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019312_33378.666666666666671120.0RepeatNote=LRR 11
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019336_35778.666666666666671120.0RepeatNote=LRR 12
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019360_38278.666666666666671120.0RepeatNote=LRR 13
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019387_40878.666666666666671120.0RepeatNote=LRR 14
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019411_43278.666666666666671120.0RepeatNote=LRR 15
TgeneLRIG3chr18:18625253chr12:59308117ENST0000032074301999_12078.666666666666671120.0RepeatNote=LRR 2
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019122_14218.6666666666666681060.0RepeatNote=LRR 3
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019146_16718.6666666666666681060.0RepeatNote=LRR 4
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019168_18918.6666666666666681060.0RepeatNote=LRR 5
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019193_21418.6666666666666681060.0RepeatNote=LRR 6
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019216_23718.6666666666666681060.0RepeatNote=LRR 7
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019240_26118.6666666666666681060.0RepeatNote=LRR 8
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019264_28518.6666666666666681060.0RepeatNote=LRR 9
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019288_30918.6666666666666681060.0RepeatNote=LRR 10
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019312_33318.6666666666666681060.0RepeatNote=LRR 11
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019336_35718.6666666666666681060.0RepeatNote=LRR 12
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019360_38218.6666666666666681060.0RepeatNote=LRR 13
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019387_40818.6666666666666681060.0RepeatNote=LRR 14
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019411_43218.6666666666666681060.0RepeatNote=LRR 15
TgeneLRIG3chr18:18625253chr12:59308117ENST0000037914101975_9618.6666666666666681060.0RepeatNote=LRR 1
TgeneLRIG3chr18:18625253chr12:59308117ENST0000037914101999_12018.6666666666666681060.0RepeatNote=LRR 2
TgeneLRIG3chr18:18625253chr12:59308117ENST00000320743019810_83078.666666666666671120.0TransmembraneHelical
TgeneLRIG3chr18:18625253chr12:59308117ENST00000379141019810_83018.6666666666666681060.0TransmembraneHelical

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-5331011_1102196.666666666666661355.0Coiled coilOntology_term=ECO:0000255
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533422_692196.666666666666661355.0Coiled coilOntology_term=ECO:0000255
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533636_980196.666666666666661355.0Compositional biasNote=Glu-rich
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-5331118_1317196.666666666666661355.0DomainPH
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533341_409196.666666666666661355.0DomainAGC-kinase C-terminal
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533479_556196.666666666666661355.0DomainREM-1
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-53376_338196.666666666666661355.0DomainProtein kinase
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533949_1015196.666666666666661355.0DomainRhoBD
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-5331115_1354196.666666666666661355.0RegionNote=Auto-inhibitory
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533707_946196.666666666666661355.0RegionSHROOM3 binding
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533998_1010196.666666666666661355.0RegionNote=RHOA binding
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-5331228_1281196.666666666666661355.0Zinc fingerPhorbol-ester/DAG-type
TgeneLRIG3chr18:18625253chr12:59308117ENST0000032074301938_7478.666666666666671120.0DomainNote=LRRNT
TgeneLRIG3chr18:18625253chr12:59308117ENST0000032074301975_9678.666666666666671120.0RepeatNote=LRR 1


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
LRIG3CAND1, TAZ, TNFSF13B, PVRIG, GINM1, LPAR6, PTPRK, PTGER3, GAL3ST1, LRIG1, LRRN2, TMPRSS3, EGFR, ERBB2, ERBB4, KIAA1429, MTDH, PSMD14, ORF3a, E, M, nsp4, nsp6, nsp9, ORF6, ORF7a, ORF7b, TRIM66, LMO7, LIMCH1, ELOVL5, GJA1, HSD17B11, METTL7A, STX4, PDE3A, CLEC12B, SPSB4, OR10H1, CLGN, FBXO2, TMPRSS13, NRSN1, RLN1, RARRES1, LRRC4C, CLEC4A, SPSB2, ANPEP, DPP4, TMPRSS4,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
ROCK1
LRIG3all structure


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with
HgeneROCK1chr18:18625253chr12:59308117ENST00000399799-533368_727196.666666666666661355.0FHOD1


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Related Drugs to ROCK1-LRIG3


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ROCK1-LRIG3


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource