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Fusion Protein:SH3PXD2A-HTRA1 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: SH3PXD2A-HTRA1 | FusionPDB ID: 81508 | FusionGDB2.0 ID: 81508 | Hgene | Tgene | Gene symbol | SH3PXD2A | HTRA1 | Gene ID | 9644 | 5654 |
| Gene name | SH3 and PX domains 2A | HtrA serine peptidase 1 | |
| Synonyms | FISH|SH3MD1|TKS5 | ARMD7|CADASIL2|CARASIL|HtrA|L56|ORF480|PRSS11 | |
| Cytomap | 10q24.33 | 10q26.13 | |
| Type of gene | protein-coding | protein-coding | |
| Description | SH3 and PX domain-containing protein 2ASH3 multiple domains 1adapter protein TKS5adaptor protein TKS5five SH3 domain-containing proteintyrosine kinase substrate with five SH3 domains | serine protease HTRA1IGFBP5-proteasehigh-temperature requirement A serine peptidase 1protease, serine, 11 (IGF binding) | |
| Modification date | 20200313 | 20200322 | |
| UniProtAcc | . | Q92743 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000315994, ENST00000427662, ENST00000355946, ENST00000369774, ENST00000538130, ENST00000540321, | ENST00000368984, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 12 X 20 X 12=2880 | 4 X 5 X 4=80 |
| # samples | 29 | 5 | |
| ** MAII score | log2(29/2880*10)=-3.31194400631474 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(5/80*10)=-0.678071905112638 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: SH3PXD2A [Title/Abstract] AND HTRA1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | SH3PXD2A(105452785)-HTRA1(124248414), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | SH3PXD2A-HTRA1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SH3PXD2A-HTRA1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SH3PXD2A-HTRA1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SH3PXD2A-HTRA1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SH3PXD2A-HTRA1 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | SH3PXD2A | GO:0006801 | superoxide metabolic process | 19755710 |
| Fusion gene breakpoints across SH3PXD2A (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across HTRA1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerKB4 | . | . | SH3PXD2A | chr10 | 105452814 | - | HTRA1 | chr10 | 105452814 | + |
| ChiTaRS5.0 | N/A | AV734257 | SH3PXD2A | chr10 | 105452785 | - | HTRA1 | chr10 | 124248414 | + |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000369774 | SH3PXD2A | chr10 | 105452785 | - | ENST00000368984 | HTRA1 | chr10 | 124248414 | + | 2224 | 704 | 202 | 1674 | 490 |
| ENST00000355946 | SH3PXD2A | chr10 | 105452785 | - | ENST00000368984 | HTRA1 | chr10 | 124248414 | + | 2087 | 567 | 65 | 1537 | 490 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000369774 | ENST00000368984 | SH3PXD2A | chr10 | 105452785 | - | HTRA1 | chr10 | 124248414 | + | 0.001724619 | 0.9982754 |
| ENST00000355946 | ENST00000368984 | SH3PXD2A | chr10 | 105452785 | - | HTRA1 | chr10 | 124248414 | + | 0.001396724 | 0.9986033 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >81508_81508_1_SH3PXD2A-HTRA1_SH3PXD2A_chr10_105452785_ENST00000355946_HTRA1_chr10_124248414_ENST00000368984_length(amino acids)=490AA_BP=166 MEKEPEPGRDEKVTPPGAPLALWGKMLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPIEGGQK DPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKRKSGQEDPNSLRHKYN FIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQG KLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAG ISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQ -------------------------------------------------------------- >81508_81508_2_SH3PXD2A-HTRA1_SH3PXD2A_chr10_105452785_ENST00000369774_HTRA1_chr10_124248414_ENST00000368984_length(amino acids)=490AA_BP=166 MEKEPEPGRDEKVTPPGAPLALWGKMLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPIEGGQK DPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKRKSGQEDPNSLRHKYN FIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQG KLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAG ISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQ -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:105452785/chr10:124248414) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | HTRA1 |
| FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. | FUNCTION: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. {ECO:0000269|PubMed:16377621, ECO:0000269|PubMed:20671064, ECO:0000269|PubMed:9852107}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 4_128 | 142.33333333333334 | 1106.0 | Domain | PX |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 4_128 | 142.33333333333334 | 1134.0 | Domain | PX |
| Tgene | HTRA1 | chr10:105452785 | chr10:124248414 | ENST00000368984 | 0 | 9 | 365_467 | 157.33333333333334 | 481.0 | Domain | PDZ | |
| Tgene | HTRA1 | chr10:105452785 | chr10:124248414 | ENST00000368984 | 0 | 9 | 204_364 | 157.33333333333334 | 481.0 | Region | Note=Serine protease |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 917_946 | 142.33333333333334 | 1106.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 917_946 | 142.33333333333334 | 1134.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 634_724 | 142.33333333333334 | 1106.0 | Compositional bias | Note=Ser-rich |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 634_724 | 142.33333333333334 | 1134.0 | Compositional bias | Note=Ser-rich |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 1072_1133 | 142.33333333333334 | 1106.0 | Domain | SH3 5 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 166_225 | 142.33333333333334 | 1106.0 | Domain | SH3 1 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 266_325 | 142.33333333333334 | 1106.0 | Domain | SH3 2 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 448_507 | 142.33333333333334 | 1106.0 | Domain | SH3 3 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000355946 | - | 6 | 14 | 840_899 | 142.33333333333334 | 1106.0 | Domain | SH3 4 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 1072_1133 | 142.33333333333334 | 1134.0 | Domain | SH3 5 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 166_225 | 142.33333333333334 | 1134.0 | Domain | SH3 1 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 266_325 | 142.33333333333334 | 1134.0 | Domain | SH3 2 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 448_507 | 142.33333333333334 | 1134.0 | Domain | SH3 3 |
| Hgene | SH3PXD2A | chr10:105452785 | chr10:124248414 | ENST00000369774 | - | 6 | 15 | 840_899 | 142.33333333333334 | 1134.0 | Domain | SH3 4 |
| Tgene | HTRA1 | chr10:105452785 | chr10:124248414 | ENST00000368984 | 0 | 9 | 33_100 | 157.33333333333334 | 481.0 | Domain | IGFBP N-terminal | |
| Tgene | HTRA1 | chr10:105452785 | chr10:124248414 | ENST00000368984 | 0 | 9 | 98_157 | 157.33333333333334 | 481.0 | Domain | Kazal-like |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| SH3PXD2A | |
| HTRA1 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to SH3PXD2A-HTRA1 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to SH3PXD2A-HTRA1 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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