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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:SSBP2-LGALS2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SSBP2-LGALS2
	FusionPDB ID: 86712
	FusionGDB2.0 ID: 86712
		Hgene	Tgene
	Gene symbol	SSBP2	LGALS2
	Gene ID	23635	3958
	Gene name	single stranded DNA binding protein 2	galectin 3
	Synonyms	HSPC116\|SOSS-B2	CBP35\|GAL3\|GALBP\|GALIG\|L31\|LGALS2\|MAC2
	Cytomap	5q14.1	14q22.3
	Type of gene	protein-coding	protein-coding
	Description	single-stranded DNA-binding protein 2sequence-specific single-stranded-DNA-binding protein 2	galectin-335 kDa lectinIgE-binding proteinMAC-2 antigenadvanced glycation end-product receptor 3carbohydrate-binding protein 35epididymis secretory sperm binding proteingalactose-specific lectin 3laminin-binding proteinlectin L-29lectin, galacto
	Modification date	20200313	20200322
	UniProtAcc	P81877	P05162
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000320672, ENST00000505980, ENST00000509053, ENST00000514493, ENST00000515395, ENST00000510060,	ENST00000215886,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	12 X 13 X 6=936	4 X 2 X 4=32
	# samples	14	6
	** MAII score	log2(14/936*10)=-2.74108170263844 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(6/32*10)=0.906890595608518 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: SSBP2 [Title/Abstract] AND LGALS2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SSBP2(80911292)-LGALS2(37967938), # samples:3
Anticipated loss of major functional domain due to fusion event.	SSBP2-LGALS2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SSBP2-LGALS2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SSBP2-LGALS2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SSBP2-LGALS2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	LGALS2	GO:0002548	monocyte chemotaxis	10925302
Tgene	LGALS2	GO:0030593	neutrophil chemotaxis	10925302
Tgene	LGALS2	GO:0031334	positive regulation of protein complex assembly	24846175
Tgene	LGALS2	GO:0045806	negative regulation of endocytosis	19706535
Tgene	LGALS2	GO:0048245	eosinophil chemotaxis	10925302
Tgene	LGALS2	GO:0048246	macrophage chemotaxis	10925302
Tgene	LGALS2	GO:0050918	positive chemotaxis	10925302
Tgene	LGALS2	GO:0070232	regulation of T cell apoptotic process	8692888
Tgene	LGALS2	GO:0071674	mononuclear cell migration	10925302
Tgene	LGALS2	GO:0071677	positive regulation of mononuclear cell migration	10925302
Tgene	LGALS2	GO:0090280	positive regulation of calcium ion import	10925302
Tgene	LGALS2	GO:1903078	positive regulation of protein localization to plasma membrane	24846175
Tgene	LGALS2	GO:2001200	positive regulation of dendritic cell differentiation	16116184
Tgene	LGALS2	GO:2001237	negative regulation of extrinsic apoptotic signaling pathway	22761016

Fusion gene breakpoints across SSBP2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across LGALS2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	SKCM	TCGA-BF-A5EQ-01A	SSBP2	chr5	80911292	-	LGALS2	chr22	37967938	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000320672	SSBP2	chr5	80911292	-	ENST00000215886	LGALS2	chr22	37967938	-	901	493	211	885	224
ENST00000509053	SSBP2	chr5	80911292	-	ENST00000215886	LGALS2	chr22	37967938	-	696	288	6	680	224
ENST00000514493	SSBP2	chr5	80911292	-	ENST00000215886	LGALS2	chr22	37967938	-	898	490	208	882	224
ENST00000505980	SSBP2	chr5	80911292	-	ENST00000215886	LGALS2	chr22	37967938	-	695	287	5	679	224
ENST00000515395	SSBP2	chr5	80911292	-	ENST00000215886	LGALS2	chr22	37967938	-	745	337	55	729	224

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000320672	ENST00000215886	SSBP2	chr5	80911292	-	LGALS2	chr22	37967938	-	0.003842891	0.9961571
ENST00000509053	ENST00000215886	SSBP2	chr5	80911292	-	LGALS2	chr22	37967938	-	0.005670892	0.99432915
ENST00000514493	ENST00000215886	SSBP2	chr5	80911292	-	LGALS2	chr22	37967938	-	0.003899202	0.9961008
ENST00000505980	ENST00000215886	SSBP2	chr5	80911292	-	LGALS2	chr22	37967938	-	0.005736857	0.9942631
ENST00000515395	ENST00000215886	SSBP2	chr5	80911292	-	LGALS2	chr22	37967938	-	0.006570608	0.99342936

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>86712_86712_1_SSBP2-LGALS2_SSBP2_chr5_80911292_ENST00000320672_LGALS2_chr22_37967938_ENST00000215886_length(amino acids)=224AA_BP=94
MYGKGKSNSSAVPSDSQAREKLALYVYEYLLHVGAQKSAQTFLSEIRWEKNITLGEPPGFLHSWWCVFWDLYCAAPERRETCEHSSEAKA
FHDYGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTF

--------------------------------------------------------------

>86712_86712_2_SSBP2-LGALS2_SSBP2_chr5_80911292_ENST00000505980_LGALS2_chr22_37967938_ENST00000215886_length(amino acids)=224AA_BP=94
MYGKGKSNSSAVPSDSQAREKLALYVYEYLLHVGAQKSAQTFLSEIRWEKNITLGEPPGFLHSWWCVFWDLYCAAPERRETCEHSSEAKA
FHDYGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTF

--------------------------------------------------------------

>86712_86712_3_SSBP2-LGALS2_SSBP2_chr5_80911292_ENST00000509053_LGALS2_chr22_37967938_ENST00000215886_length(amino acids)=224AA_BP=94
MYGKGKSNSSAVPSDSQAREKLALYVYEYLLHVGAQKSAQTFLSEIRWEKNITLGEPPGFLHSWWCVFWDLYCAAPERRETCEHSSEAKA
FHDYGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTF

--------------------------------------------------------------

>86712_86712_4_SSBP2-LGALS2_SSBP2_chr5_80911292_ENST00000514493_LGALS2_chr22_37967938_ENST00000215886_length(amino acids)=224AA_BP=94
MYGKGKSNSSAVPSDSQAREKLALYVYEYLLHVGAQKSAQTFLSEIRWEKNITLGEPPGFLHSWWCVFWDLYCAAPERRETCEHSSEAKA
FHDYGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTF

--------------------------------------------------------------

>86712_86712_5_SSBP2-LGALS2_SSBP2_chr5_80911292_ENST00000515395_LGALS2_chr22_37967938_ENST00000215886_length(amino acids)=224AA_BP=94
MYGKGKSNSSAVPSDSQAREKLALYVYEYLLHVGAQKSAQTFLSEIRWEKNITLGEPPGFLHSWWCVFWDLYCAAPERRETCEHSSEAKA
FHDYGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTF

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:80911292/chr22:37967938)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
SSBP2 P81877	LGALS2 P05162
	FUNCTION: This protein binds beta-galactoside. Its physiological function is not yet known.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000320672

18_50

94.0

362.0

Domain

LisH

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000505980

18_50

94.0

342.0

Domain

LisH

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000509053

18_50

94.0

299.0

Domain

LisH

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000514493

18_50

94.0

332.0

Domain

LisH

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000515395

18_50

94.0

340.0

Domain

LisH

Tgene

LGALS2

chr5:80911292

chr22:37967938

ENST00000215886

4_131

2.0

133.0

Domain

Galectin

Tgene

LGALS2

chr5:80911292

chr22:37967938

ENST00000215886

65_71

2.0

133.0

Region

Beta-galactoside binding

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000320672

100_293

94.0

362.0

Compositional bias

Note=Pro-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000320672

147_312

94.0

362.0

Compositional bias

Note=Gly-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000505980

100_293

94.0

342.0

Compositional bias

Note=Pro-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000505980

147_312

94.0

342.0

Compositional bias

Note=Gly-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000509053

100_293

94.0

299.0

Compositional bias

Note=Pro-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000509053

147_312

94.0

299.0

Compositional bias

Note=Gly-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000514493

100_293

94.0

332.0

Compositional bias

Note=Pro-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000514493

147_312

94.0

332.0

Compositional bias

Note=Gly-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000515395

100_293

94.0

340.0

Compositional bias

Note=Pro-rich

Hgene

SSBP2

chr5:80911292

chr22:37967938

ENST00000515395

147_312

94.0

340.0

Compositional bias

Note=Gly-rich

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

SSBP2

EWSR1, LDB1, IL36RN, TAL1, LMO2, TRIM33, DYRK2, CDK6, BAG3, TOM1, LDB2, LMX1B, IDI2, ISL2, ISL1, SSBP4, SSBP3, CLEC18A, LMO4, LHX4, LHX6, PIN1, ARHGAP22, ARHGEF10, CAMK2A, FPR1, RRD1, CBFA2T3, NABP1, INTS3, MYH9, ACACA, PC, MCCC2, MCCC1, EP300, PYGO1, BCL9, ORC6, BCL9L, LHX3, MFAP4, LMO1, LMO3, PICK1, VIM, S100A7A, HLA-DRB1, HMOX1, NDRG2, LCP1, IGHG1, IGHG2, TYMP, HIST1H2AG, HLA-DRA, PPL, IL36G, HIST1H1B, NDRG1, IVL, TREX2, IL1RN, GLUL, EVPL, SERPINA1, MNDA, TF, TRIM29, SERPINB3, SERPINB4, SERPINB2, C3, HLA-DQB1, HLA-DPB1, LGALS7B, FGB, SERPINB7, CBR1, ALOX15, IGKC, STAT3, SULT2B1, CALML3, PYCARD, CALML5, IGLL5, H2AFY, T, GATA2, LHX1, LHX2, LHX8, TEAD1, TLX1,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
SSBP2
LGALS2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to SSBP2-LGALS2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to SSBP2-LGALS2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	SSBP2	C0005586	Bipolar Disorder	1	CTD_human
Hgene	SSBP2	C0005587	Depression, Bipolar	1	CTD_human
Hgene	SSBP2	C0024713	Manic Disorder	1	CTD_human
Hgene	SSBP2	C0033578	Prostatic Neoplasms	1	CTD_human
Hgene	SSBP2	C0338831	Manic	1	CTD_human
Hgene	SSBP2	C0376358	Malignant neoplasm of prostate	1	CTD_human