UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level1

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:BAG4-WRN

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: BAG4-WRN
	FusionPDB ID: 8864
	FusionGDB2.0 ID: 8864
		Hgene	Tgene
	Gene symbol	BAG4	WRN
	Gene ID	9530	7486
	Gene name	BAG cochaperone 4	WRN RecQ like helicase
	Synonyms	BAG-4\|SODD	RECQ3\|RECQL2\|RECQL3
	Cytomap	8p11.23	8p12
	Type of gene	protein-coding	protein-coding
	Description	BAG family molecular chaperone regulator 4BCL2 associated athanogene 4bcl-2-associated athanogene 4silencer of death domains	Werner syndrome ATP-dependent helicaseDNA helicase, RecQ-like type 3Werner syndrome RecQ like helicaseWerner syndrome, RecQ helicase-likeexonuclease WRNrecQ protein-like 2
	Modification date	20200313	20200320
	UniProtAcc	O95429	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000521282, ENST00000287322, ENST00000432471,	ENST00000298139,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	12 X 5 X 7=420	9 X 10 X 7=630
	# samples	15	11
	** MAII score	log2(15/420*10)=-1.48542682717024 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(11/630*10)=-2.51784830486262 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: BAG4 [Title/Abstract] AND WRN [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	BAG4(38050313)-WRN(31012140), # samples:3
Anticipated loss of major functional domain due to fusion event.	BAG4-WRN seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BAG4-WRN seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	BAG4	GO:0071356	cellular response to tumor necrosis factor	21712384
Tgene	WRN	GO:0000731	DNA synthesis involved in DNA repair	17563354
Tgene	WRN	GO:0006259	DNA metabolic process	16622405
Tgene	WRN	GO:0006284	base-excision repair	17611195
Tgene	WRN	GO:0006974	cellular response to DNA damage stimulus	18203716
Tgene	WRN	GO:0006979	response to oxidative stress	17611195
Tgene	WRN	GO:0009267	cellular response to starvation	11420665
Tgene	WRN	GO:0010225	response to UV-C	17563354
Tgene	WRN	GO:0031297	replication fork processing	17115688
Tgene	WRN	GO:0032508	DNA duplex unwinding	11735402\|26420422
Tgene	WRN	GO:0044806	G-quadruplex DNA unwinding	11735402
Tgene	WRN	GO:0051345	positive regulation of hydrolase activity	17611195
Tgene	WRN	GO:0061820	telomeric D-loop disassembly	15200954\|19734539\|26420422
Tgene	WRN	GO:0071480	cellular response to gamma radiation	21639834
Tgene	WRN	GO:0098530	positive regulation of strand invasion	26420422
Tgene	WRN	GO:1902570	protein localization to nucleolus	11420665

Fusion gene breakpoints across BAG4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across WRN (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BLCA	TCGA-FD-A62O-01A	BAG4	chr8	38050313	-	WRN	chr8	31012140	+
ChimerDB4	BLCA	TCGA-FD-A62O-01A	BAG4	chr8	38050313	+	WRN	chr8	31012140	+
ChimerDB4	BLCA	TCGA-FD-A62O	BAG4	chr8	38050313	+	WRN	chr8	31012139	+

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000287322

BAG4

chr8

38050313

ENST00000298139

WRN

chr8

31012140

1928

649

148

1260

370

ENST00000287322

BAG4

chr8

38050313

ENST00000298139

WRN

chr8

31012139

1928

649

148

1260

370

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000287322	ENST00000298139	BAG4	chr8	38050313	+	WRN	chr8	31012140	+	0.000737102	0.99926287
ENST00000287322	ENST00000298139	BAG4	chr8	38050313	+	WRN	chr8	31012139	+	0.000737102	0.99926287

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>8864_8864_1_BAG4-WRN_BAG4_chr8_38050313_ENST00000287322_WRN_chr8_31012139_ENST00000298139_length(amino acids)=370AA_BP=167
MGLAGVVRPPSLLSIRNRPRAPARWVGRGERGGKRFRAADPMSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPPLYPLRPEPPQPPIS
WRVRGGGPAETTWLGEGGGGDGYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNSTARSRAPYPSTYPVRPELQGQTDLFSSTKPQEEQ
KTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKI
SLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPGSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLM

--------------------------------------------------------------

>8864_8864_2_BAG4-WRN_BAG4_chr8_38050313_ENST00000287322_WRN_chr8_31012140_ENST00000298139_length(amino acids)=370AA_BP=167
MGLAGVVRPPSLLSIRNRPRAPARWVGRGERGGKRFRAADPMSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPPLYPLRPEPPQPPIS
WRVRGGGPAETTWLGEGGGGDGYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNSTARSRAPYPSTYPVRPELQGQTDLFSSTKPQEEQ
KTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKI
SLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPGSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLM

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:38050313/chr8:31012140)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
BAG4 O95429	.
FUNCTION: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. Negative regulator of PRKN translocation to damaged mitochondria. {ECO:0000250, ECO:0000269\|PubMed:24270810}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

BAG4

chr8:38050313

chr8:31012139

ENST00000287322

379_456

126.0

458.0

Domain

BAG

Hgene

BAG4

chr8:38050313

chr8:31012139

ENST00000432471

379_456

422.0

Domain

BAG

Hgene

BAG4

chr8:38050313

chr8:31012140

ENST00000287322

379_456

126.0

458.0

Domain

BAG

Hgene

BAG4

chr8:38050313

chr8:31012140

ENST00000432471

379_456

422.0

Domain

BAG

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

507_510

1229.0

1433.0

Compositional bias

Note=Poly-Glu

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

507_510

1229.0

1433.0

Compositional bias

Note=Poly-Glu

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

1150_1229

1229.0

1433.0

Domain

HRDC

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

558_724

1229.0

1433.0

Domain

Helicase ATP-binding

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

60_228

1229.0

1433.0

Domain

Note=3'-5' exonuclease

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

749_899

1229.0

1433.0

Domain

Helicase C-terminal

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

1150_1229

1229.0

1433.0

Domain

HRDC

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

558_724

1229.0

1433.0

Domain

Helicase ATP-binding

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

60_228

1229.0

1433.0

Domain

Note=3'-5' exonuclease

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

749_899

1229.0

1433.0

Domain

Helicase C-terminal

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

668_671

1229.0

1433.0

Motif

Note=DEAH box

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

668_671

1229.0

1433.0

Motif

Note=DEAH box

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

571_578

1229.0

1433.0

Nucleotide binding

ATP

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

571_578

1229.0

1433.0

Nucleotide binding

ATP

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

424_477

1229.0

1433.0

Region

Note=2 X 27 AA tandem repeats of H-L-S-P-N-D-N-E-N-D-T-S-Y-V-I-E-S-D-E-D-L-E-M-E-M-L-K

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

424_477

1229.0

1433.0

Region

Note=2 X 27 AA tandem repeats of H-L-S-P-N-D-N-E-N-D-T-S-Y-V-I-E-S-D-E-D-L-E-M-E-M-L-K

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

424_450

1229.0

1433.0

Repeat

Tgene

WRN

chr8:38050313

chr8:31012139

ENST00000298139

451_477

1229.0

1433.0

Repeat

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

424_450

1229.0

1433.0

Repeat

Tgene

WRN

chr8:38050313

chr8:31012140

ENST00000298139

451_477

1229.0

1433.0

Repeat

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
BAG4
WRN

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	WRN	chr8:38050313	chr8:31012139	ENST00000298139		30	35	987_993	1229.0	1433.0	DNA
Tgene	WRN	chr8:38050313	chr8:31012140	ENST00000298139		30	35	987_993	1229.0	1433.0	DNA
Tgene	WRN	chr8:38050313	chr8:31012139	ENST00000298139		30	35	2_277	1229.0	1433.0	WRNIP1
Tgene	WRN	chr8:38050313	chr8:31012140	ENST00000298139		30	35	2_277	1229.0	1433.0	WRNIP1

Top

Related Drugs to BAG4-WRN

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to BAG4-WRN

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source