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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:TNKS-MSRA

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: TNKS-MSRA
	FusionPDB ID: 92756
	FusionGDB2.0 ID: 92756
		Hgene	Tgene
	Gene symbol	TNKS	MSRA
	Gene ID	8658	4482
	Gene name	tankyrase	methionine sulfoxide reductase A
	Synonyms	ARTD5\|PARP-5a\|PARP5A\|PARPL\|TIN1\|TINF1\|TNKS1\|pART5	PMSR
	Cytomap	8p23.1	8p23.1
	Type of gene	protein-coding	protein-coding
	Description	poly [ADP-ribose] polymerase tankyrase-1ADP-ribosyltransferase diphtheria toxin-like 5TANK1TNKS-1TRF1-interacting ankyrin-related ADP-ribose polymerase 1poly [ADP-ribose] polymerase 5Aprotein poly-ADP-ribosyltransferase tankyrase-1tankyrase Itanky	mitochondrial peptide methionine sulfoxide reductasecytosolic methionine-S-sulfoxide reductasepeptide Met(O) reductasepeptide met (O) reductasepeptide-methionine (S)-S-oxide reductase
	Modification date	20200313	20200313
	UniProtAcc	.	Q9UJ68
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000518027, ENST00000310430, ENST00000518281, ENST00000520408, ENST00000522110,	ENST00000317173, ENST00000441698, ENST00000518255, ENST00000382490, ENST00000521209, ENST00000528246, ENST00000517594,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	13 X 8 X 8=832	11 X 9 X 5=495
	# samples	14	12
	** MAII score	log2(14/832*10)=-2.57115670119613 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(12/495*10)=-2.04439411935845 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: TNKS [Title/Abstract] AND MSRA [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	TNKS(9473187)-MSRA(10065343), # samples:2
Anticipated loss of major functional domain due to fusion event.	TNKS-MSRA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TNKS-MSRA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TNKS-MSRA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. TNKS-MSRA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	TNKS	GO:0000209	protein polyubiquitination	19759537\|21478859
Hgene	TNKS	GO:0006471	protein ADP-ribosylation	9822378\|15133513\|21270334
Hgene	TNKS	GO:0018105	peptidyl-serine phosphorylation	17026964
Hgene	TNKS	GO:0018107	peptidyl-threonine phosphorylation	17026964
Hgene	TNKS	GO:0032212	positive regulation of telomere maintenance via telomerase	12782650\|18221737
Hgene	TNKS	GO:0045944	positive regulation of transcription by RNA polymerase II	19245366
Hgene	TNKS	GO:0051973	positive regulation of telomerase activity	12782650
Hgene	TNKS	GO:0070212	protein poly-ADP-ribosylation	9822378\|16076287\|25043379
Hgene	TNKS	GO:0070213	protein auto-ADP-ribosylation	9822378\|15133513\|19759537\|21478859
Hgene	TNKS	GO:1904355	positive regulation of telomere capping	21531765
Hgene	TNKS	GO:1904743	negative regulation of telomeric DNA binding	9822378

Fusion gene breakpoints across TNKS (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across MSRA (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	READ	TCGA-EI-6885-01A	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+
ChimerDB4	READ	TCGA-EI-6885	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+
ChimerDB4	READ	TCGA-EI-6885	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000520408	TNKS	chr8	9473187	+	ENST00000317173	MSRA	chr8	10065343	+	2165	1020	26	1585	519
ENST00000520408	TNKS	chr8	9473187	+	ENST00000441698	MSRA	chr8	10065343	+	1519	1020	26	1465	479
ENST00000520408	TNKS	chr8	9473187	+	ENST00000518255	MSRA	chr8	10065343	+	1536	1020	26	1519	497
ENST00000310430	TNKS	chr8	9473187	+	ENST00000317173	MSRA	chr8	10065343	+	2165	1020	26	1585	519
ENST00000310430	TNKS	chr8	9473187	+	ENST00000441698	MSRA	chr8	10065343	+	1519	1020	26	1465	479
ENST00000310430	TNKS	chr8	9473187	+	ENST00000518255	MSRA	chr8	10065343	+	1536	1020	26	1519	497
ENST00000518281	TNKS	chr8	9473187	+	ENST00000317173	MSRA	chr8	10065343	+	1859	714	431	1279	282
ENST00000518281	TNKS	chr8	9473187	+	ENST00000441698	MSRA	chr8	10065343	+	1213	714	431	1159	242
ENST00000518281	TNKS	chr8	9473187	+	ENST00000518255	MSRA	chr8	10065343	+	1230	714	431	1213	260
ENST00000520408	TNKS	chr8	9473187	+	ENST00000317173	MSRA	chr8	10065342	+	2165	1020	26	1585	519
ENST00000520408	TNKS	chr8	9473187	+	ENST00000441698	MSRA	chr8	10065342	+	1519	1020	26	1465	479
ENST00000520408	TNKS	chr8	9473187	+	ENST00000518255	MSRA	chr8	10065342	+	1536	1020	26	1519	497
ENST00000310430	TNKS	chr8	9473187	+	ENST00000317173	MSRA	chr8	10065342	+	2165	1020	26	1585	519
ENST00000310430	TNKS	chr8	9473187	+	ENST00000441698	MSRA	chr8	10065342	+	1519	1020	26	1465	479
ENST00000310430	TNKS	chr8	9473187	+	ENST00000518255	MSRA	chr8	10065342	+	1536	1020	26	1519	497
ENST00000518281	TNKS	chr8	9473187	+	ENST00000317173	MSRA	chr8	10065342	+	1859	714	431	1279	282
ENST00000518281	TNKS	chr8	9473187	+	ENST00000441698	MSRA	chr8	10065342	+	1213	714	431	1159	242
ENST00000518281	TNKS	chr8	9473187	+	ENST00000518255	MSRA	chr8	10065342	+	1230	714	431	1213	260

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000520408	ENST00000317173	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.003909055	0.99609095
ENST00000520408	ENST00000441698	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.010783921	0.9892161
ENST00000520408	ENST00000518255	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.02458325	0.9754167
ENST00000310430	ENST00000317173	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.003909055	0.99609095
ENST00000310430	ENST00000441698	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.010783921	0.9892161
ENST00000310430	ENST00000518255	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.02458325	0.9754167
ENST00000518281	ENST00000317173	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.001737226	0.9982628
ENST00000518281	ENST00000441698	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.00273728	0.9972627
ENST00000518281	ENST00000518255	TNKS	chr8	9473187	+	MSRA	chr8	10065343	+	0.004419603	0.9955804
ENST00000520408	ENST00000317173	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.003909055	0.99609095
ENST00000520408	ENST00000441698	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.010783921	0.9892161
ENST00000520408	ENST00000518255	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.02458325	0.9754167
ENST00000310430	ENST00000317173	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.003909055	0.99609095
ENST00000310430	ENST00000441698	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.010783921	0.9892161
ENST00000310430	ENST00000518255	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.02458325	0.9754167
ENST00000518281	ENST00000317173	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.001737226	0.9982628
ENST00000518281	ENST00000441698	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.00273728	0.9972627
ENST00000518281	ENST00000518255	TNKS	chr8	9473187	+	MSRA	chr8	10065342	+	0.004419603	0.9955804

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>92756_92756_1_TNKS-MSRA_TNKS_chr8_9473187_ENST00000310430_MSRA_chr8_10065342_ENST00000317173_length(amino acids)=519AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_2_TNKS-MSRA_TNKS_chr8_9473187_ENST00000310430_MSRA_chr8_10065342_ENST00000441698_length(amino acids)=479AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFEKTGHA
EVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAED

--------------------------------------------------------------

>92756_92756_3_TNKS-MSRA_TNKS_chr8_9473187_ENST00000310430_MSRA_chr8_10065342_ENST00000518255_length(amino acids)=497AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_4_TNKS-MSRA_TNKS_chr8_9473187_ENST00000310430_MSRA_chr8_10065343_ENST00000317173_length(amino acids)=519AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_5_TNKS-MSRA_TNKS_chr8_9473187_ENST00000310430_MSRA_chr8_10065343_ENST00000441698_length(amino acids)=479AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFEKTGHA
EVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAED

--------------------------------------------------------------

>92756_92756_6_TNKS-MSRA_TNKS_chr8_9473187_ENST00000310430_MSRA_chr8_10065343_ENST00000518255_length(amino acids)=497AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_7_TNKS-MSRA_TNKS_chr8_9473187_ENST00000518281_MSRA_chr8_10065342_ENST00000317173_length(amino acids)=282AA_BP=0
MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAK
AVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEEL
LKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKNPNGYCGLG

--------------------------------------------------------------

>92756_92756_8_TNKS-MSRA_TNKS_chr8_9473187_ENST00000518281_MSRA_chr8_10065342_ENST00000441698_length(amino acids)=242AA_BP=0
MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAK
AVLTAKHHVNGNRTVEPFPEGTQMAVFEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAAL

--------------------------------------------------------------

>92756_92756_9_TNKS-MSRA_TNKS_chr8_9473187_ENST00000518281_MSRA_chr8_10065342_ENST00000518255_length(amino acids)=260AA_BP=0
MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAK
AVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEEL

--------------------------------------------------------------

>92756_92756_10_TNKS-MSRA_TNKS_chr8_9473187_ENST00000518281_MSRA_chr8_10065343_ENST00000317173_length(amino acids)=282AA_BP=0
MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAK
AVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEEL
LKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKNPNGYCGLG

--------------------------------------------------------------

>92756_92756_11_TNKS-MSRA_TNKS_chr8_9473187_ENST00000518281_MSRA_chr8_10065343_ENST00000441698_length(amino acids)=242AA_BP=0
MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAK
AVLTAKHHVNGNRTVEPFPEGTQMAVFEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAAL

--------------------------------------------------------------

>92756_92756_12_TNKS-MSRA_TNKS_chr8_9473187_ENST00000518281_MSRA_chr8_10065343_ENST00000518255_length(amino acids)=260AA_BP=0
MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAK
AVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEEL

--------------------------------------------------------------

>92756_92756_13_TNKS-MSRA_TNKS_chr8_9473187_ENST00000520408_MSRA_chr8_10065342_ENST00000317173_length(amino acids)=519AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_14_TNKS-MSRA_TNKS_chr8_9473187_ENST00000520408_MSRA_chr8_10065342_ENST00000441698_length(amino acids)=479AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFEKTGHA
EVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAED

--------------------------------------------------------------

>92756_92756_15_TNKS-MSRA_TNKS_chr8_9473187_ENST00000520408_MSRA_chr8_10065342_ENST00000518255_length(amino acids)=497AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_16_TNKS-MSRA_TNKS_chr8_9473187_ENST00000520408_MSRA_chr8_10065343_ENST00000317173_length(amino acids)=519AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

>92756_92756_17_TNKS-MSRA_TNKS_chr8_9473187_ENST00000520408_MSRA_chr8_10065343_ENST00000441698_length(amino acids)=479AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFEKTGHA
EVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAED

--------------------------------------------------------------

>92756_92756_18_TNKS-MSRA_TNKS_chr8_9473187_ENST00000520408_MSRA_chr8_10065343_ENST00000518255_length(amino acids)=497AA_BP=0
MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC
STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS
GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC
QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW
GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:9473187/chr8:10065343)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	MSRA Q9UJ68
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

109_161

331.3333333333333

1328.0

Compositional bias

Ser-rich

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

20_83

331.3333333333333

1328.0

Compositional bias

Pro-rich

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

9_14

331.3333333333333

1328.0

Compositional bias

Poly-His

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

109_161

331.3333333333333

1328.0

Compositional bias

Ser-rich

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

20_83

331.3333333333333

1328.0

Compositional bias

Pro-rich

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

9_14

331.3333333333333

1328.0

Compositional bias

Poly-His

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

181_209

331.3333333333333

1328.0

Repeat

ANK 1

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

215_244

331.3333333333333

1328.0

Repeat

ANK 2

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

248_277

331.3333333333333

1328.0

Repeat

ANK 3

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

281_310

331.3333333333333

1328.0

Repeat

ANK 4

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

181_209

331.3333333333333

1328.0

Repeat

ANK 1

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

215_244

331.3333333333333

1328.0

Repeat

ANK 2

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

248_277

331.3333333333333

1328.0

Repeat

ANK 3

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

281_310

331.3333333333333

1328.0

Repeat

ANK 4

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

1030_1089

331.3333333333333

1328.0

Domain

SAM

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

1112_1317

331.3333333333333

1328.0

Domain

PARP catalytic

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

1030_1089

331.3333333333333

1328.0

Domain

SAM

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

1112_1317

331.3333333333333

1328.0

Domain

PARP catalytic

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

335_364

331.3333333333333

1328.0

Repeat

ANK 5

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

368_397

331.3333333333333

1328.0

Repeat

ANK 6

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

401_430

331.3333333333333

1328.0

Repeat

ANK 7

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

434_463

331.3333333333333

1328.0

Repeat

ANK 8

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

521_553

331.3333333333333

1328.0

Repeat

ANK 9

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

557_586

331.3333333333333

1328.0

Repeat

ANK 10

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

590_619

331.3333333333333

1328.0

Repeat

ANK 11

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

621_647

331.3333333333333

1328.0

Repeat

ANK 12

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

649_679

331.3333333333333

1328.0

Repeat

ANK 13

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

683_712

331.3333333333333

1328.0

Repeat

ANK 14

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

716_745

331.3333333333333

1328.0

Repeat

ANK 15

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

749_778

331.3333333333333

1328.0

Repeat

ANK 16

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

782_810

331.3333333333333

1328.0

Repeat

ANK 17

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

836_865

331.3333333333333

1328.0

Repeat

ANK 18

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

869_898

331.3333333333333

1328.0

Repeat

ANK 19

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

902_931

331.3333333333333

1328.0

Repeat

ANK 20

Hgene

TNKS

chr8:9473187

chr8:10065342

ENST00000310430

935_964

331.3333333333333

1328.0

Repeat

ANK 21

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

335_364

331.3333333333333

1328.0

Repeat

ANK 5

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

368_397

331.3333333333333

1328.0

Repeat

ANK 6

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

401_430

331.3333333333333

1328.0

Repeat

ANK 7

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

434_463

331.3333333333333

1328.0

Repeat

ANK 8

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

521_553

331.3333333333333

1328.0

Repeat

ANK 9

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

557_586

331.3333333333333

1328.0

Repeat

ANK 10

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

590_619

331.3333333333333

1328.0

Repeat

ANK 11

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

621_647

331.3333333333333

1328.0

Repeat

ANK 12

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

649_679

331.3333333333333

1328.0

Repeat

ANK 13

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

683_712

331.3333333333333

1328.0

Repeat

ANK 14

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

716_745

331.3333333333333

1328.0

Repeat

ANK 15

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

749_778

331.3333333333333

1328.0

Repeat

ANK 16

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

782_810

331.3333333333333

1328.0

Repeat

ANK 17

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

836_865

331.3333333333333

1328.0

Repeat

ANK 18

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

869_898

331.3333333333333

1328.0

Repeat

ANK 19

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

902_931

331.3333333333333

1328.0

Repeat

ANK 20

Hgene

TNKS

chr8:9473187

chr8:10065343

ENST00000310430

935_964

331.3333333333333

1328.0

Repeat

ANK 21

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1108_TNKS_9473187_MSRA_10065343_1108_TNKS_9473187_MSRA_10065343_ranked_0.pdb	TNKS	9473187	9473187	ENST00000518255	MSRA	chr8	10065343	+	MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCC STTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVS GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLC QGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTAKHHVNGNRTVEPFPEGTQMAVFGMGCFW GAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSA	519

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

TNKS_pLDDT.png

MSRA_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
TNKS
MSRA

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to TNKS-MSRA

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to TNKS-MSRA

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source