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level3

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Potential Active Site Information
	Potentially Interacting Small Molecules through Virtual Screening
	Biochemical Features of Small Molecules with ADME
	Drug Toxicity Information
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:TPM3-JAK2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: TPM3-JAK2
	FusionPDB ID: 93381
	FusionGDB2.0 ID: 93381
		Hgene	Tgene
	Gene symbol	TPM3	JAK2
	Gene ID	7170	3717
	Gene name	tropomyosin 3	Janus kinase 2
	Synonyms	CAPM1\|CFTD\|HEL-189\|HEL-S-82p\|NEM1\|OK/SW-cl.5\|TM-5\|TM3\|TM30\|TM30nm\|TM5\|TPM3nu\|TPMsk3\|TRK\|hscp30	JTK10\|THCYT3
	Cytomap	1q21.3	9p24.1
	Type of gene	protein-coding	protein-coding
	Description	tropomyosin alpha-3 chainalpha-tropomyosin, slow skeletalcytoskeletal tropomyosin TM30epididymis luminal protein 189epididymis secretory sperm binding protein Li 82pheat-stable cytoskeletal protein 30 kDatropomyosin 3 nutropomyosin gammatropomyosi	tyrosine-protein kinase JAK2JAK-2Janus kinase 2 (a protein tyrosine kinase)
	Modification date	20200328	20200329
	UniProtAcc	P06753	O60674
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000271850, ENST00000302206, ENST00000323144, ENST00000328159, ENST00000330188, ENST00000341372, ENST00000341485, ENST00000368530, ENST00000368531, ENST00000368533, ENST00000469717,	ENST00000487310, ENST00000381652, ENST00000539801, ENST00000544510,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	15 X 22 X 8=2640	22 X 26 X 9=5148
	# samples	24	15
	** MAII score	log2(24/2640*10)=-3.4594316186373 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(15/5148*10)=-5.10097764772482 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: TPM3 [Title/Abstract] AND JAK2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)
Anticipated loss of major functional domain due to fusion event.	TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a kinase by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a kinase by not retaining the major functional domain in the partially deleted in-frame ORF. TPM3-JAK2 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. TPM3-JAK2 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. TPM3-JAK2 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. TPM3-JAK2 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. TPM3-JAK2 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	JAK2	GO:0010811	positive regulation of cell-substrate adhesion	10925297
Tgene	JAK2	GO:0019221	cytokine-mediated signaling pathway	8609418
Tgene	JAK2	GO:0033209	tumor necrosis factor-mediated signaling pathway	8609418
Tgene	JAK2	GO:0034612	response to tumor necrosis factor	8609418
Tgene	JAK2	GO:0035409	histone H3-Y41 phosphorylation	19783980
Tgene	JAK2	GO:0035722	interleukin-12-mediated signaling pathway	7528775
Tgene	JAK2	GO:0046677	response to antibiotic	16280321
Tgene	JAK2	GO:0050727	regulation of inflammatory response	10925297
Tgene	JAK2	GO:0060396	growth hormone receptor signaling pathway	10925297
Tgene	JAK2	GO:0070671	response to interleukin-12	7528775

Fusion gene breakpoints across TPM3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across JAK2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerKB4	.	.	TPM3	chr1	154142876		JAK2	chr9	5080229

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000368533	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		2008	717	53	1984	643
ENST00000368533	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3377	717	53	1984	643
ENST00000368533	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2043	717	53	1984	643
ENST00000330188	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		2041	750	86	2017	643
ENST00000330188	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3410	750	86	2017	643
ENST00000330188	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2076	750	86	2017	643
ENST00000341485	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		1996	705	89	1972	627
ENST00000341485	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3365	705	89	1972	627
ENST00000341485	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2031	705	89	1972	627
ENST00000341372	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		1942	651	62	1918	618
ENST00000341372	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3311	651	62	1918	618
ENST00000341372	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		1977	651	62	1918	618
ENST00000328159	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		1955	664	0	1931	643
ENST00000328159	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3324	664	0	1931	643
ENST00000328159	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		1990	664	0	1931	643
ENST00000271850	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		2106	815	34	2082	682
ENST00000271850	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3475	815	34	2082	682
ENST00000271850	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2141	815	34	2082	682
ENST00000368531	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		1966	675	11	1942	643
ENST00000368531	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3335	675	11	1942	643
ENST00000368531	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2001	675	11	1942	643
ENST00000323144	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		2000	709	45	1976	643
ENST00000323144	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3369	709	45	1976	643
ENST00000323144	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2035	709	45	1976	643
ENST00000368530	TPM3	chr1	154142876		ENST00000539801	JAK2	chr9	5080229		2259	968	187	2235	682
ENST00000368530	TPM3	chr1	154142876		ENST00000381652	JAK2	chr9	5080229		3628	968	187	2235	682
ENST00000368530	TPM3	chr1	154142876		ENST00000544510	JAK2	chr9	5080229		2294	968	187	2235	682

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

No-coding score

Coding score

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>93381_93381_1_TPM3-JAK2_TPM3_chr1_154142876_ENST00000271850_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=682AA_BP=258
MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVAS
LNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLE
RTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVP
PECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFT
PDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRV
KIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIE

--------------------------------------------------------------

>93381_93381_2_TPM3-JAK2_TPM3_chr1_154142876_ENST00000271850_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=682AA_BP=258
MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVAS
LNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLE
RTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVP
PECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFT
PDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRV
KIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIE

--------------------------------------------------------------

>93381_93381_3_TPM3-JAK2_TPM3_chr1_154142876_ENST00000271850_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=682AA_BP=258
MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVAS
LNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLE
RTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVP
PECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFT
PDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRV
KIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIE

--------------------------------------------------------------

>93381_93381_4_TPM3-JAK2_TPM3_chr1_154142876_ENST00000323144_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_5_TPM3-JAK2_TPM3_chr1_154142876_ENST00000323144_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_6_TPM3-JAK2_TPM3_chr1_154142876_ENST00000323144_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_7_TPM3-JAK2_TPM3_chr1_154142876_ENST00000328159_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_8_TPM3-JAK2_TPM3_chr1_154142876_ENST00000328159_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_9_TPM3-JAK2_TPM3_chr1_154142876_ENST00000328159_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_10_TPM3-JAK2_TPM3_chr1_154142876_ENST00000330188_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_11_TPM3-JAK2_TPM3_chr1_154142876_ENST00000330188_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_12_TPM3-JAK2_TPM3_chr1_154142876_ENST00000330188_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_13_TPM3-JAK2_TPM3_chr1_154142876_ENST00000341372_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=618AA_BP=194
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGESRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEA
KHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEF
AERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLI
NNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDN
TGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGM
EYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKS

--------------------------------------------------------------

>93381_93381_14_TPM3-JAK2_TPM3_chr1_154142876_ENST00000341372_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=618AA_BP=194
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGESRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEA
KHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEF
AERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLI
NNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDN
TGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGM
EYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKS

--------------------------------------------------------------

>93381_93381_15_TPM3-JAK2_TPM3_chr1_154142876_ENST00000341372_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=618AA_BP=194
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGESRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEA
KHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEF
AERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLI
NNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDN
TGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGM
EYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKS

--------------------------------------------------------------

>93381_93381_16_TPM3-JAK2_TPM3_chr1_154142876_ENST00000341485_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=627AA_BP=203
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADEEKME
LQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKL
KEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAP
KWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEM
CRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQ
YTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLY

--------------------------------------------------------------

>93381_93381_17_TPM3-JAK2_TPM3_chr1_154142876_ENST00000341485_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=627AA_BP=203
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADEEKME
LQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKL
KEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAP
KWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEM
CRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQ
YTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLY

--------------------------------------------------------------

>93381_93381_18_TPM3-JAK2_TPM3_chr1_154142876_ENST00000341485_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=627AA_BP=203
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADEEKME
LQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKL
KEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAP
KWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEM
CRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQ
YTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLY

--------------------------------------------------------------

>93381_93381_19_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368530_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=682AA_BP=258
MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVAS
LNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLE
RTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVP
PECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFT
PDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRV
KIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIE

--------------------------------------------------------------

>93381_93381_20_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368530_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=682AA_BP=258
MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVAS
LNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLE
RTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVP
PECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFT
PDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRV
KIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIE

--------------------------------------------------------------

>93381_93381_21_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368530_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=682AA_BP=258
MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVAS
LNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLE
RTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVP
PECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFT
PDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRV
KIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIE

--------------------------------------------------------------

>93381_93381_22_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368531_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_23_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368531_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_24_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368531_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_25_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368533_JAK2_chr9_5080229_ENST00000381652_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_26_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368533_JAK2_chr9_5080229_ENST00000539801_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

>93381_93381_27_TPM3-JAK2_TPM3_chr1_154142876_ENST00000368533_JAK2_chr9_5080229_ENST00000544510_length(amino acids)=643AA_BP=219
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERG
MKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDS
QRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHL
KFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD
YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTES
KFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDL

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:/chr9:)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
TPM3 P06753	JAK2 O60674
FUNCTION: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. {ECO:0000250\|UniProtKB:P09493}.	FUNCTION: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980). {ECO:0000269\|PubMed:12023369, ECO:0000269\|PubMed:19783980, ECO:0000269\|PubMed:20098430, ECO:0000269\|PubMed:21368206, ECO:0000269\|PubMed:21423214, ECO:0000269\|PubMed:7615558, ECO:0000269\|PubMed:9618263}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000323144

16_44

221.33333333333334

248.0

Region

Disordered

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000330188

16_44

221.33333333333334

249.0

Region

Disordered

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000368530

16_44

258.3333333333333

286.0

Region

Disordered

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000368531

16_44

221.33333333333334

248.0

Region

Disordered

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000368533

16_44

221.33333333333334

249.0

Region

Disordered

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000381652

849_1124

710.3333333333334

1133.0

Domain

Protein kinase 2

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000539801

849_1124

710.3333333333334

1133.0

Domain

Protein kinase 2

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000381652

855_863

710.3333333333334

1133.0

Nucleotide binding

ATP

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000539801

855_863

710.3333333333334

1133.0

Nucleotide binding

ATP

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000323144

1_285

221.33333333333334

248.0

Coiled coil

Ontology_term=ECO:0000250

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000330188

1_285

221.33333333333334

249.0

Coiled coil

Ontology_term=ECO:0000250

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000368530

1_285

258.3333333333333

286.0

Coiled coil

Ontology_term=ECO:0000250

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000368531

1_285

221.33333333333334

248.0

Coiled coil

Ontology_term=ECO:0000250

Hgene

TPM3

chr1:154142876

chr9:5080229

ENST00000368533

1_285

221.33333333333334

249.0

Coiled coil

Ontology_term=ECO:0000250

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000381652

37_380

710.3333333333334

1133.0

Domain

FERM

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000381652

401_482

710.3333333333334

1133.0

Domain

SH2%3B atypical

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000381652

545_809

710.3333333333334

1133.0

Domain

Protein kinase 1

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000539801

37_380

710.3333333333334

1133.0

Domain

FERM

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000539801

401_482

710.3333333333334

1133.0

Domain

SH2%3B atypical

Tgene

JAK2

chr1:154142876

chr9:5080229

ENST00000539801

545_809

710.3333333333334

1133.0

Domain

Protein kinase 1

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)

PDB file (489) >>>489.pdbFusion protein BP residue: 194 CIF file (489) >>>489.cif	TPM3	chr1	154142876		JAK2	chr9	5080229		MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGESRAQERLATALQ KLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRK YEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAE EKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEVLQE RIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQ FYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYE LLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVE MCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYK GVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGM EYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPG ESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRM IGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRP	618
3D view using mol* of 489 (AA BP:194)


PDB file (500) >>>500.pdbFusion protein BP residue: 203 CIF file (500) >>>500.cif	TPM3	chr1	154142876		JAK2	chr9	5080229		MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVA SLNRRIQLVEEELDRAQERLATALQKLEEAEKAADEEKMELQEIQLKEAK HIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQ NLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKT IDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLS ALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDL NSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQL GKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSL QHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQ YTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDK EYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSK SPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTEC	627
3D view using mol* of 500 (AA BP:203)


PDB file (516) >>>516.pdbFusion protein BP residue: 219 CIF file (516) >>>516.cif	TPM3	chr1	154142876		JAK2	chr9	5080229		MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVA SLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALK DEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELA ESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAE TRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSF GTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMD YEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDR DPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTE EHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENR VKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWS FGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLP	643
3D view using mol* of 516 (AA BP:219)


PDB file (517) >>>517.pdbFusion protein BP residue: 219 CIF file (517) >>>517.cif	TPM3	chr1	154142876		JAK2	chr9	5080229		MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVA SLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALK DEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELA ESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAE TRAEFAERSVAKLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSF GTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMD YEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDR DPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTE EHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRD YLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENR VKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWS FGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLP	643
3D view using mol* of 517 (AA BP:219)


PDB file (551) >>>551.pdbFusion protein BP residue: 258 CIF file (551) >>>551.cif	TPM3	chr1	154142876		JAK2	chr9	5080229		MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQ KKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEE ELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQ LKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQI RLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVA KLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGG DKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRA IIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLK FLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDH IKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKV LPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTY IEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYM	682
3D view using mol* of 551 (AA BP:258)


PDB file (552) >>>552.pdbFusion protein BP residue: 258 CIF file (552) >>>552.cif	TPM3	chr1	154142876		JAK2	chr9	5080229		MLMMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQ KKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEE ELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQ LKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEEL KNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVA KLEKTIDDLEVLQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGG DKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRA IIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLK FLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDH IKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKV LPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTY IEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYM	682
3D view using mol* of 552 (AA BP:258)

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

TPM3_pLDDT.png

JAK2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

TPM3_JAK2_489_pLDDT.png (AA BP:194)

TPM3_JAK2_489_pLDDT_and_active_sites.png (AA BP:194)

TPM3_JAK2_489_violinplot.png (AA BP:194)

TPM3_JAK2_500_pLDDT.png (AA BP:203)

TPM3_JAK2_500_pLDDT_and_active_sites.png (AA BP:203)

TPM3_JAK2_500_violinplot.png (AA BP:203)

TPM3_JAK2_516_pLDDT.png (AA BP:219)

TPM3_JAK2_516_pLDDT_and_active_sites.png (AA BP:219)

TPM3_JAK2_516_violinplot.png (AA BP:219)

TPM3_JAK2_517_pLDDT.png (AA BP:219)

TPM3_JAK2_517_pLDDT_and_active_sites.png (AA BP:219)

TPM3_JAK2_517_violinplot.png (AA BP:219)

TPM3_JAK2_551_pLDDT.png (AA BP:258)

TPM3_JAK2_552_pLDDT.png (AA BP:258)

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

TPM3_JAK2_489.png

TPM3_JAK2_500.png

TPM3_JAK2_516.png

TPM3_JAK2_517.png

TPM3_JAK2_551.png

TPM3_JAK2_552.png

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Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Fusion AA seq ID in FusionPDB	Site score	Size	D score	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
489	1.018	360	1.014	985.439	0.528	0.724	0.918	0.717	1.106	0.649	0.71	Chain A: 341,342,343,344,345,346,347,348,349,366,3 68,370,376,377,379,380,381,383,384,387,388,397,413 ,415,416,417,418,419,420,421,422,425,429,461,462,4 66,467,469,479,480,481,482,483,484,485,488,491,493 ,494,495,496,497,498,499,500,501,502,503,504,507,5 11,512,513,514,515,517,561
500	1.023	335	1.033	973.777	0.54	0.732	0.931	0.653	1.06	0.616	0.689	Chain A: 350,351,352,353,354,355,356,357,358,375,3 77,379,385,386,389,390,392,393,397,406,424,425,426 ,427,429,430,431,434,438,470,471,475,476,478,488,4 89,490,491,492,493,494,497,500,502,503,504,505,506 ,507,508,509,510,511,512,513,516,520,521,524,526,5 67,570,574
500	1.033	308	1.051	874.993	0.538	0.742	0.94	0.82	1.023	0.801	0.765	Chain A: 350,351,352,353,354,355,356,357,358,375,3 77,379,385,386,389,390,392,393,397,406,424,425,426 ,427,428,429,430,431,434,438,470,471,475,476,478,4 88,489,490,491,492,493,494,505,507,508,509,510,511 ,512,513,516,520,521,522,524,526,570
516	1.029	315	1.048	929.53	0.51	0.736	0.953	0.785	1.025	0.766	0.804	Chain A: 366,367,369,370,371,372,373,374,391,393,3 95,401,402,404,405,406,408,409,413,422,438,440,441 ,442,443,445,446,447,450,454,486,487,491,492,494,5 04,505,506,507,508,509,510,513,516,518,519,520,521 ,522,523,524,525,526,527,528,529,532,536,537,540,5 42,583,586,590
517	1.057	254	1.043	774.494	0.46	0.784	0.996	0.985	1.127	0.874	0.622	Chain A: 366,367,368,369,370,371,372,373,374,391,3 93,395,401,402,404,405,406,408,409,413,422,438,440 ,441,442,443,446,447,450,485,486,487,491,492,494,5 04,505,506,507,508,509,510,519,521,522,523,524,525 ,526,527,532

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Potentially Interacting Small Molecules through Virtual Screening

The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.

Fusion AA seq ID in FusionPDB

ZINC ID

DrugBank ID

Drug name

Docking score

Glide gscore

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Drug information from DrugBank of the top 20 interacting small molecules.

ZINC ID

DrugBank ID

Drug name

Drug type

SMILES

Drug group

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Biochemical Features of Small Molecules

ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)

ZINC ID

mol_MW

dipole

SASA

FOSA

FISA

PISA

WPSA

volume

donorHB

accptHB

Human Oral Absorption

Percent Human Oral Absorption

Rule Of Five

Rule Of Three

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Drug Toxicity Information

Toxicity information of individual drugs using eToxPred

ZINC ID

Smile

Surface Accessibility

Toxicity

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene	PPI interactors
JAK2	PRMT5, SOCS1, PPIA, IL12RB2, EPOR, FYN, STAT5A, STAT5B, SH2B2, CXCR4, DNAJA3, HSPA8, TEC, PTPN12, PTK2, CSF2RB, GHR, VAV1, SIRPA, IRS1, BRCA1, GRB2, EGFR, PTK2B, PTPN11, TNFRSF1A, SOCS3, PTPN6, LYN, HTR2A, ERBB2, ELP2, KIT, RAF1, SH2B1, IL5RA, PPP2R1B, IFNGR1, IFNGR2, CCR5, PRLR, IL6ST, SHC1, GRB10, TUB, TSHR, STAT3, INSR, STAM, PTPN1, STAM2, IL2RB, CTLA4, ALK, IKBKG, FGFR1, STAT1, HSP90AA1, HSP90AB1, ASB2, SKP2, GTF2I, HIST3H3, HIST2H3C, CDKN1B, VHL, PIK3R1, MAP3K5, AGTR1, PTAFR, JAK2, IL4R, IL2RG, PPP1CC, ERBB3, CALM1, SRC, JAK3, PLCG1, HES1, HES5, Ifngr1, ASS1, RBMX, TRAF6, EZH2, DTX3L, EMD, KPNB1, NAP1L1, RCN1, SLC2A1, UBP1, ARL11, HSFY1, HDAC6, HNRNPL, CBL, RNF123, Lmo4, GOLPH3, IGF1R, PARP9, LNX1, LDOC1, CSK, MPL, CUL4A, CUL4B, M, JAK1, AMY1C, BPIFA2, ARHGAP18, BACH1, IGHA2, HMMR, MUC7, CST2, IGJ, PBK, MUC5B, SPRR3, ZG16B, VCP,
TPM3	KXD1, TFPT, MCC, EIF4A2, TPM3, TP73, NEK2, RAD21, ELAVL1, SP1, CUL3, CDK2, LRRK2, TPM4, SEC23IP, KYNU, TPM2, TPM1, PRDX1, THOC1, CDC123, SF3A2, SEPT9, HSPB1, BRCA1, MDC1, PAXIP1, HSP90AA1, HSP90AB1, FN1, PAN2, HDAC8, FBXO5, TRAP1, PARK2, FASN, FKBP4, FKBP5, MAGED2, CAP1, MCM3, MGEA5, TP53, HSF2, IFIT3, KCNE1, KIFC3, MAGEA11, PBX3, TRIM27, VPS52, TNNT1, TRIP6, MAD1L1, TLK1, OIP5, BLOC1S6, CCHCR1, THAP1, CCDC146, CCDC102B, CCDC114, SYCE1, IKBIP, C1orf216, LCA5L, FAM9C, HDDC3, WASH3P, LURAP1, SHMT2, MOV10, NXF1, CUL7, OBSL1, RNF2, EGFR, DAPK1, ACTN2, AHCYL1, ATP1A1, CKB, CKMT1B, CKMT1A, COX5A, CYC1, OSTF1, ACTN1, ACTN4, ATP5F1, COX4I1, MTCH1, MYH7B, NDUFB10, PSMA2, PSMA6, STMN1, TBCD, UQCRC2, NTRK1, DVL2, HOOK2, ADD1, CAPZA2, MYH9, PPP1CB, PPP1CC, IQGAP1, PDLIM7, ANLN, MYO5C, MYO19, MYO18A, Actb, Itga5, Myh9, Tpm1, Mis12, Calml3, Myh10, Tpm4, MCM2, RNF20, IMP3, CALCOCO1, JUN, METTL2B, CCDC101, SPAG9, PARK7, PDHA1, VDAC1, TMPO, CTNNB1, LARS, APOE, HDAC4, RXRA, EFTUD2, AAR2, PIH1D1, RPTOR, TNF, FGF11, NOD2, RNF181, AGR2, GPC1, KDM6B, MYC, CDK9, Prkaa1, Prkab1, METTL14, WTAP, ESR2, TET2, DISC1, NR2C2, CSK, AGRN, HOOK3, BMH1, BMH2, VMP1, SNRNP70, BIRC3, ATXN1, TAB2, PSMC5, HSF4, TSKS, NUP54, SNAPIN, TNNI1, PRKCDBP, FGA, PHLDB3, WASH1, THAP7, PLEKHA4, RAD18, PRKD1, SRC, HIST2H2BE, GFM1, MN1, ESR1, MAST2, UNK, DUX4, CIT, AURKB, CHRM3, ECT2, KIF14, KIF20A, SMARCA2, ARHGAP11A, SYDE2, ARHGAP39, Ophn1, MFN2, OPA1, UCHL1, Apc2, FBP1, DTNA, INSIG1, INSIG2, RIN3, TRIM24, PDIA4, SUMO2, MARCKS, CALM1, NEDD4, TKT, CALR, SOD1, FKBP3, TRIM33, RBM8A, PEBP1, PGAM1, CEP78, PTMS, DDX58, CD274, AR, ISG15, UFL1, TMOD1, TRIM37, HTRA4, NAA40, CCDC102A, CCDC6, EXOC4, RBMXL1, TSSC4, KIF5B, EAPP, TPM3P9, PTRF, ZFC3H1, CROCC, NDE1, CCDC96, JUND, ECD, PHF21A, NUF2, NDEL1, HSF1, HOMER2, PRPF8, RCOR1, KDM1A, HOMER1, RCOR3, NES, JUNB, BTF3, TRIM26, FBXW7, DPP4, E, nsp7, ORF7b, ORF8, S, FURIN, nsp4, nsp6, ORF10, CCNF, ANTXR1, FOS, GATA4, KLF10, KLF3, KLF9, ZIC3, NKX2-5, STAT5B,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
TPM3
JAK2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	JAK2	chr1:154142876	chr9:5080229	ENST00000381652		15	25	1_239	710.3333333333334	1133.0	cytokine/interferon/growth hormone receptors
Tgene	JAK2	chr1:154142876	chr9:5080229	ENST00000539801		14	24	1_239	710.3333333333334	1133.0	cytokine/interferon/growth hormone receptors

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Related Drugs to TPM3-JAK2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to TPM3-JAK2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	TPM3	C1836448	Nemaline myopathy 1	9	CTD_human;GENOMICS_ENGLAND;UNIPROT
Hgene	TPM3	C0546264	Congenital Fiber Type Disproportion	4	CTD_human;GENOMICS_ENGLAND;UNIPROT
Hgene	TPM3	C0007097	Carcinoma	2	CTD_human
Hgene	TPM3	C0024667	Animal Mammary Neoplasms	2	CTD_human
Hgene	TPM3	C0024668	Mammary Neoplasms, Experimental	2	CTD_human
Hgene	TPM3	C0205696	Anaplastic carcinoma	2	CTD_human
Hgene	TPM3	C0205697	Carcinoma, Spindle-Cell	2	CTD_human
Hgene	TPM3	C0205698	Undifferentiated carcinoma	2	CTD_human
Hgene	TPM3	C0205699	Carcinomatosis	2	CTD_human
Hgene	TPM3	C0206157	Myopathies, Nemaline	2	GENOMICS_ENGLAND
Hgene	TPM3	C1257925	Mammary Carcinoma, Animal	2	CTD_human
Hgene	TPM3	C0001418	Adenocarcinoma	1	CTD_human
Hgene	TPM3	C0007102	Malignant tumor of colon	1	CTD_human
Hgene	TPM3	C0009375	Colonic Neoplasms	1	CTD_human
Hgene	TPM3	C0023893	Liver Cirrhosis, Experimental	1	CTD_human
Hgene	TPM3	C0024623	Malignant neoplasm of stomach	1	CTD_human
Hgene	TPM3	C0038356	Stomach Neoplasms	1	CTD_human
Hgene	TPM3	C0040136	Thyroid Neoplasm	1	CTD_human
Hgene	TPM3	C0151468	Thyroid Gland Follicular Adenoma	1	CTD_human
Hgene	TPM3	C0205641	Adenocarcinoma, Basal Cell	1	CTD_human
Hgene	TPM3	C0205642	Adenocarcinoma, Oxyphilic	1	CTD_human
Hgene	TPM3	C0205643	Carcinoma, Cribriform	1	CTD_human
Hgene	TPM3	C0205644	Carcinoma, Granular Cell	1	CTD_human
Hgene	TPM3	C0205645	Adenocarcinoma, Tubular	1	CTD_human
Hgene	TPM3	C0270960	Congenital myopathy (disorder)	1	GENOMICS_ENGLAND
Hgene	TPM3	C0334121	Inflammatory Myofibroblastic Tumor	1	ORPHANET
Hgene	TPM3	C0546125	Nemaline Myopathy, Childhood Onset	1	ORPHANET
Hgene	TPM3	C0549473	Thyroid carcinoma	1	CTD_human
Hgene	TPM3	C1708349	Hereditary Diffuse Gastric Cancer	1	CTD_human
Hgene	TPM3	C3710589	Cap Myopathy	1	ORPHANET
Tgene	JAK2	C0032463	Polycythemia Vera	12	CTD_human;ORPHANET;UNIPROT
Tgene	JAK2	C0040028	Thrombocythemia, Essential	10	CTD_human;ORPHANET
Tgene	JAK2	C0001815	Primary Myelofibrosis	9	CGI;CTD_human;GENOMICS_ENGLAND;ORPHANET
Tgene	JAK2	C3489628	Thrombocytosis, Autosomal Dominant	8	CTD_human
Tgene	JAK2	C0019154	Hepatic Vein Thrombosis	3	CTD_human;ORPHANET
Tgene	JAK2	C0856761	Budd-Chiari Syndrome	3	CTD_human;ORPHANET
Tgene	JAK2	C0009324	Ulcerative Colitis	2	CTD_human
Tgene	JAK2	C0027022	Myeloproliferative disease	2	CTD_human
Tgene	JAK2	C0151744	Myocardial Ischemia	2	CTD_human
Tgene	JAK2	C0836924	Thrombocytosis	2	CTD_human
Tgene	JAK2	C3281125	THROMBOCYTHEMIA 3	2	UNIPROT
Tgene	JAK2	C0000786	Spontaneous abortion	1	CTD_human
Tgene	JAK2	C0000822	Abortion, Tubal	1	CTD_human
Tgene	JAK2	C0006663	Calcinosis	1	CTD_human
Tgene	JAK2	C0007873	Uterine Cervical Neoplasm	1	CTD_human
Tgene	JAK2	C0018824	Heart valve disease	1	CTD_human
Tgene	JAK2	C0019207	Hepatoma, Morris	1	CTD_human
Tgene	JAK2	C0019208	Hepatoma, Novikoff	1	CTD_human
Tgene	JAK2	C0021368	Inflammation	1	CTD_human
Tgene	JAK2	C0023418	leukemia	1	CTD_human
Tgene	JAK2	C0023467	Leukemia, Myelocytic, Acute	1	CGI;CTD_human;UNIPROT
Tgene	JAK2	C0023893	Liver Cirrhosis, Experimental	1	CTD_human
Tgene	JAK2	C0023904	Liver Neoplasms, Experimental	1	CTD_human
Tgene	JAK2	C0025472	Mesenteric Vascular Occlusion	1	CTD_human
Tgene	JAK2	C0026998	Acute Myeloid Leukemia, M1	1	CTD_human
Tgene	JAK2	C0032461	Polycythemia	1	CTD_human
Tgene	JAK2	C0032962	Pregnancy Complications	1	CTD_human
Tgene	JAK2	C0033578	Prostatic Neoplasms	1	CTD_human
Tgene	JAK2	C0040038	Thromboembolism	1	CTD_human
Tgene	JAK2	C0042487	Venous Thrombosis	1	CTD_human
Tgene	JAK2	C0086404	Experimental Hepatoma	1	CTD_human
Tgene	JAK2	C0149871	Deep Vein Thrombosis	1	CTD_human
Tgene	JAK2	C0263628	Tumoral calcinosis	1	CTD_human
Tgene	JAK2	C0376358	Malignant neoplasm of prostate	1	CTD_human
Tgene	JAK2	C0400966	Non-alcoholic Fatty Liver Disease	1	CTD_human
Tgene	JAK2	C0521174	Microcalcification	1	CTD_human
Tgene	JAK2	C1527405	Erythrocytosis	1	CTD_human
Tgene	JAK2	C1879321	Acute Myeloid Leukemia (AML-M2)	1	CTD_human
Tgene	JAK2	C3241937	Nonalcoholic Steatohepatitis	1	CTD_human
Tgene	JAK2	C3495676	Anorectal Malformations	1	GENOMICS_ENGLAND
Tgene	JAK2	C3830362	Early Pregnancy Loss	1	CTD_human
Tgene	JAK2	C4048328	cervical cancer	1	CTD_human
Tgene	JAK2	C4303761	Familial thrombocytosis	1	ORPHANET
Tgene	JAK2	C4552766	Miscarriage	1	CTD_human