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Fusion Protein:UBE2D2-HINT1 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: UBE2D2-HINT1 | FusionPDB ID: 96014 | FusionGDB2.0 ID: 96014 | Hgene | Tgene | Gene symbol | UBE2D2 | HINT1 | Gene ID | 7322 | 3094 |
| Gene name | ubiquitin conjugating enzyme E2 D2 | histidine triad nucleotide binding protein 1 | |
| Synonyms | E2(17)KB2|PUBC1|UBC4|UBC4/5|UBCH4|UBCH5B | HINT|NMAN|PKCI-1|PRKCNH1 | |
| Cytomap | 5q31.2 | 5q23.3 | |
| Type of gene | protein-coding | protein-coding | |
| Description | ubiquitin-conjugating enzyme E2 D2(E3-independent) E2 ubiquitin-conjugating enzyme D2E2 ubiquitin-conjugating enzyme D2p53-regulated ubiquitin-conjugating enzyme 1ubiquitin carrier protein D2ubiquitin conjugating enzyme E2D 2ubiquitin-conjugating en | histidine triad nucleotide-binding protein 1adenosine 5'-monophosphoramidaseepididymis secretory sperm binding proteinprotein kinase C inhibitor 1protein kinase C-interacting protein 1 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | . | P49773 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000253815, ENST00000398733, ENST00000505548, ENST00000511725, | ENST00000506207, ENST00000506908, ENST00000508488, ENST00000513012, ENST00000304043, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 18 X 9 X 11=1782 | 8 X 9 X 6=432 |
| # samples | 23 | 10 | |
| ** MAII score | log2(23/1782*10)=-2.95379157057755 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(10/432*10)=-2.11103131238874 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: UBE2D2 [Title/Abstract] AND HINT1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | UBE2D2(138994202)-HINT1(130495304), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | UBE2D2-HINT1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. UBE2D2-HINT1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. UBE2D2-HINT1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. UBE2D2-HINT1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | UBE2D2 | GO:0000209 | protein polyubiquitination | 15247280 |
| Hgene | UBE2D2 | GO:0016567 | protein ubiquitination | 9990509|14593114 |
| Hgene | UBE2D2 | GO:0051865 | protein autoubiquitination | 21068390 |
| Hgene | UBE2D2 | GO:0070936 | protein K48-linked ubiquitination | 20061386 |
| Tgene | HINT1 | GO:0009154 | purine ribonucleotide catabolic process | 16835243 |
| Fusion gene breakpoints across UBE2D2 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across HINT1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | BRCA | TCGA-EW-A1OV | UBE2D2 | chr5 | 138994202 | + | HINT1 | chr5 | 130495304 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000511725 | UBE2D2 | chr5 | 138994202 | + | ENST00000304043 | HINT1 | chr5 | 130495304 | - | 863 | 278 | 306 | 1 | 102 |
| ENST00000253815 | UBE2D2 | chr5 | 138994202 | + | ENST00000304043 | HINT1 | chr5 | 130495304 | - | 1495 | 910 | 439 | 1074 | 211 |
| ENST00000398733 | UBE2D2 | chr5 | 138994202 | + | ENST00000304043 | HINT1 | chr5 | 130495304 | - | 1331 | 746 | 478 | 56 | 140 |
| ENST00000505548 | UBE2D2 | chr5 | 138994202 | + | ENST00000304043 | HINT1 | chr5 | 130495304 | - | 1060 | 475 | 64 | 639 | 191 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000511725 | ENST00000304043 | UBE2D2 | chr5 | 138994202 | + | HINT1 | chr5 | 130495304 | - | 0.8082786 | 0.19172138 |
| ENST00000253815 | ENST00000304043 | UBE2D2 | chr5 | 138994202 | + | HINT1 | chr5 | 130495304 | - | 0.5089654 | 0.49103463 |
| ENST00000398733 | ENST00000304043 | UBE2D2 | chr5 | 138994202 | + | HINT1 | chr5 | 130495304 | - | 0.21419843 | 0.7858016 |
| ENST00000505548 | ENST00000304043 | UBE2D2 | chr5 | 138994202 | + | HINT1 | chr5 | 130495304 | - | 0.9046853 | 0.09531466 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >96014_96014_1_UBE2D2-HINT1_UBE2D2_chr5_138994202_ENST00000253815_HINT1_chr5_130495304_ENST00000304043_length(amino acids)=211AA_BP=157 MRQAQELSGPRPSARPAGPAFLNSPSSPADRDRRGGLRLPSPFPVPSPPPSPPRGPPPPASHHGSEENPQAPSTKPPELGLTEEAVLPDP QVYPEFTYLLGGSTHRSTLLVQKLLRVGSSEEKKKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPLLGHLMIVGKKCAADLGLNKGYR -------------------------------------------------------------- >96014_96014_2_UBE2D2-HINT1_UBE2D2_chr5_138994202_ENST00000398733_HINT1_chr5_130495304_ENST00000304043_length(amino acids)=140AA_BP= MRAGAHSAPGPAAAVTASPGGGPFIRRRPSRHRRRRRSCLFLPGSAPPPKPPQIPPPESKSPSLGLGRPLLVGQPEGLPVFLPAAGGPRD -------------------------------------------------------------- >96014_96014_3_UBE2D2-HINT1_UBE2D2_chr5_138994202_ENST00000505548_HINT1_chr5_130495304_ENST00000304043_length(amino acids)=191AA_BP=137 MGLPLHLRAIVGPGWRGWGRGGRWGVGRPLRQRSKGLLAPFLFPPPTPALAPSTKPPELGLTEEAVLPDPQVYPEFTYLLGGSTHRSTLL VQKLLRVGSSEEKKKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHV -------------------------------------------------------------- >96014_96014_4_UBE2D2-HINT1_UBE2D2_chr5_138994202_ENST00000511725_HINT1_chr5_130495304_ENST00000304043_length(amino acids)=102AA_BP= MPTIIKCPRSGPIIVACQWNISSPTGPAEHCAGGSRARSFNSKFQMSGLPSASSFPVFSHCVNPPRGPATRCSGEAFHQGNFLPGSALWI -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:138994202/chr5:130495304) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | HINT1 |
| FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. | FUNCTION: Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. {ECO:0000269|PubMed:15703176, ECO:0000269|PubMed:16014379, ECO:0000269|PubMed:16835243, ECO:0000269|PubMed:19112177, ECO:0000269|PubMed:22329685, ECO:0000269|PubMed:22647378, ECO:0000269|PubMed:9323207}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | HINT1 | chr5:138994202 | chr5:130495304 | ENST00000304043 | 1 | 3 | 110_114 | 72.0 | 127.0 | Motif | Note=Histidine triad motif | |
| Tgene | HINT1 | chr5:138994202 | chr5:130495304 | ENST00000304043 | 1 | 3 | 105_107 | 72.0 | 127.0 | Nucleotide binding | Note=Purine nucleotide phosphoramidate | |
| Tgene | HINT1 | chr5:138994202 | chr5:130495304 | ENST00000304043 | 1 | 3 | 112_114 | 72.0 | 127.0 | Nucleotide binding | Note=Purine nucleotide phosphoramidate |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | UBE2D2 | chr5:138994202 | chr5:130495304 | ENST00000253815 | + | 4 | 8 | 1_147 | 11.0 | 119.0 | Domain | UBC core |
| Hgene | UBE2D2 | chr5:138994202 | chr5:130495304 | ENST00000398733 | + | 3 | 7 | 1_147 | 40.0 | 148.0 | Domain | UBC core |
| Hgene | UBE2D2 | chr5:138994202 | chr5:130495304 | ENST00000505548 | + | 3 | 7 | 1_147 | 11.0 | 119.0 | Domain | UBC core |
| Hgene | UBE2D2 | chr5:138994202 | chr5:130495304 | ENST00000511725 | + | 3 | 7 | 1_147 | 11.0 | 119.0 | Domain | UBC core |
| Tgene | HINT1 | chr5:138994202 | chr5:130495304 | ENST00000304043 | 1 | 3 | 18_126 | 72.0 | 127.0 | Domain | HIT | |
| Tgene | HINT1 | chr5:138994202 | chr5:130495304 | ENST00000304043 | 1 | 3 | 43_44 | 72.0 | 127.0 | Nucleotide binding | Note=Purine nucleotide phosphoramidate |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| UBE2D2 | |
| HINT1 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to UBE2D2-HINT1 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to UBE2D2-HINT1 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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