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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:USP8-TSPAN3

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: USP8-TSPAN3
	FusionPDB ID: 97647
	FusionGDB2.0 ID: 97647
		Hgene	Tgene
	Gene symbol	USP8	TSPAN3
	Gene ID	9101	10099
	Gene name	ubiquitin specific peptidase 8	tetraspanin 3
	Synonyms	HumORF8\|PITA4\|SPG59\|UBPY	TM4-A\|TM4SF8\|TSPAN-3
	Cytomap	15q21.2	15q24.3
	Type of gene	protein-coding	protein-coding
	Description	ubiquitin carboxyl-terminal hydrolase 8deubiquitinating enzyme 8ubiquitin isopeptidase Yubiquitin thiolesterase 8ubiquitin-specific-processing protease 8	tetraspanin-3tetraspan 3tetraspan TM4SFtetraspanin TM4-Atransmembrane 4 superfamily member 8
	Modification date	20200322	20200322
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000307179, ENST00000396444, ENST00000425032, ENST00000433963, ENST00000558892,	ENST00000558394, ENST00000558745, ENST00000561277, ENST00000424443, ENST00000559494, ENST00000267970, ENST00000346495,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	10 X 10 X 8=800	12 X 7 X 9=756
	# samples	11	13
	** MAII score	log2(11/800*10)=-2.86249647625006 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(13/756*10)=-2.53987461119262 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: USP8 [Title/Abstract] AND TSPAN3 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	USP8(50763992)-TSPAN3(77348597), # samples:3
Anticipated loss of major functional domain due to fusion event.	USP8-TSPAN3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. USP8-TSPAN3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. USP8-TSPAN3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. USP8-TSPAN3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	USP8	GO:0070536	protein K63-linked deubiquitination	16520378
Hgene	USP8	GO:0071108	protein K48-linked deubiquitination	16520378

Fusion gene breakpoints across USP8 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TSPAN3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	SARC	TCGA-DX-A6YZ-01A	USP8	chr15	50763992	-	TSPAN3	chr15	77348597	-
ChimerDB4	SARC	TCGA-DX-A6YZ-01A	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-
ChimerDB4	SARC	TCGA-DX-A6YZ	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000307179	USP8	chr15	50763992	+	ENST00000267970	TSPAN3	chr15	77348597	-	4564	1047	198	1745	515
ENST00000307179	USP8	chr15	50763992	+	ENST00000346495	TSPAN3	chr15	77348597	-	2476	1047	198	1670	490
ENST00000433963	USP8	chr15	50763992	+	ENST00000267970	TSPAN3	chr15	77348597	-	4866	1349	500	2047	515
ENST00000433963	USP8	chr15	50763992	+	ENST00000346495	TSPAN3	chr15	77348597	-	2778	1349	500	1972	490
ENST00000396444	USP8	chr15	50763992	+	ENST00000267970	TSPAN3	chr15	77348597	-	4704	1187	338	1885	515
ENST00000396444	USP8	chr15	50763992	+	ENST00000346495	TSPAN3	chr15	77348597	-	2616	1187	338	1810	490
ENST00000425032	USP8	chr15	50763992	+	ENST00000267970	TSPAN3	chr15	77348597	-	4328	811	193	1509	438
ENST00000425032	USP8	chr15	50763992	+	ENST00000346495	TSPAN3	chr15	77348597	-	2240	811	193	1434	413

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000307179	ENST00000267970	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000362041	0.99963796
ENST00000307179	ENST00000346495	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000448166	0.99955183
ENST00000433963	ENST00000267970	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000378697	0.9996213
ENST00000433963	ENST00000346495	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000457104	0.99954295
ENST00000396444	ENST00000267970	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000365883	0.99963415
ENST00000396444	ENST00000346495	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000436623	0.9995634
ENST00000425032	ENST00000267970	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000366144	0.9996338
ENST00000425032	ENST00000346495	USP8	chr15	50763992	+	TSPAN3	chr15	77348597	-	0.000370871	0.99962914

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>97647_97647_1_USP8-TSPAN3_USP8_chr15_50763992_ENST00000307179_TSPAN3_chr15_77348597_ENST00000267970_length(amino acids)=515AA_BP=283
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL
GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK
GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI
GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEV
VVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAH

--------------------------------------------------------------

>97647_97647_2_USP8-TSPAN3_USP8_chr15_50763992_ENST00000307179_TSPAN3_chr15_77348597_ENST00000346495_length(amino acids)=490AA_BP=283
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL
GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK
GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI
GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATVENEVDRSIQKVY
KTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMHVI

--------------------------------------------------------------

>97647_97647_3_USP8-TSPAN3_USP8_chr15_50763992_ENST00000396444_TSPAN3_chr15_77348597_ENST00000267970_length(amino acids)=515AA_BP=283
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL
GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK
GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI
GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEV
VVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAH

--------------------------------------------------------------

>97647_97647_4_USP8-TSPAN3_USP8_chr15_50763992_ENST00000396444_TSPAN3_chr15_77348597_ENST00000346495_length(amino acids)=490AA_BP=283
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL
GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK
GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI
GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATVENEVDRSIQKVY
KTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMHVI

--------------------------------------------------------------

>97647_97647_5_USP8-TSPAN3_USP8_chr15_50763992_ENST00000425032_TSPAN3_chr15_77348597_ENST00000267970_length(amino acids)=438AA_BP=206
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKS
NGEKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVV
LLDWFSSAKDLQIGTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLAT
FVIILLLVFVTEVVVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCC

--------------------------------------------------------------

>97647_97647_6_USP8-TSPAN3_USP8_chr15_50763992_ENST00000425032_TSPAN3_chr15_77348597_ENST00000346495_length(amino acids)=413AA_BP=206
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKS
NGEKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVV
LLDWFSSAKDLQIGTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLAT
VENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAHPSDLYAEGCEAL

--------------------------------------------------------------

>97647_97647_7_USP8-TSPAN3_USP8_chr15_50763992_ENST00000433963_TSPAN3_chr15_77348597_ENST00000267970_length(amino acids)=515AA_BP=283
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL
GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK
GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI
GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEV
VVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAH

--------------------------------------------------------------

>97647_97647_8_USP8-TSPAN3_USP8_chr15_50763992_ENST00000433963_TSPAN3_chr15_77348597_ENST00000346495_length(amino acids)=490AA_BP=283
MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL
GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK
GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI
GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATVENEVDRSIQKVY
KTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMHVI

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr15:50763992/chr15:77348597)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000307179

33_116

283.0

1119.0

Domain

Note=MIT

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000396444

33_116

283.0

1119.0

Domain

Note=MIT

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000433963

33_116

283.0

1119.0

Domain

Note=MIT

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

107_212

21.0

254.0

Topological domain

Extracellular

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

234_253

21.0

254.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

33_50

21.0

254.0

Topological domain

Extracellular

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

72_85

21.0

254.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

107_212

21.0

229.0

Topological domain

Extracellular

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

234_253

21.0

229.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

33_50

21.0

229.0

Topological domain

Extracellular

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

72_85

21.0

229.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

107_212

190.0

Topological domain

Extracellular

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

1_11

190.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

234_253

190.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

33_50

190.0

Topological domain

Extracellular

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

72_85

190.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

213_233

21.0

254.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

51_71

21.0

254.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

86_106

21.0

254.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

213_233

21.0

229.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

51_71

21.0

229.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

86_106

21.0

229.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

12_32

190.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

213_233

190.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

51_71

190.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000424443

86_106

190.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000307179

195_313

283.0

1119.0

Domain

Rhodanese

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000307179

777_1109

283.0

1119.0

Domain

Note=USP

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000396444

195_313

283.0

1119.0

Domain

Rhodanese

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000396444

777_1109

283.0

1119.0

Domain

Note=USP

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000433963

195_313

283.0

1119.0

Domain

Rhodanese

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000433963

777_1109

283.0

1119.0

Domain

Note=USP

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000307179

405_413

283.0

1119.0

Motif

SH3-binding

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000396444

405_413

283.0

1119.0

Motif

SH3-binding

Hgene

USP8

chr15:50763992

chr15:77348597

ENST00000433963

405_413

283.0

1119.0

Motif

SH3-binding

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

1_11

21.0

254.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

1_11

21.0

229.0

Topological domain

Cytoplasmic

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000267970

12_32

21.0

254.0

Transmembrane

Helical

Tgene

TSPAN3

chr15:50763992

chr15:77348597

ENST00000346495

12_32

21.0

229.0

Transmembrane

Helical

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1095_USP8_50763992_TSPAN3_77348597_1095_USP8_50763992_TSPAN3_77348597_ranked_0.pdb	USP8	50763992	50763992	ENST00000346495	TSPAN3	chr15	77348597	-	MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSIL GPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEK GAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQI GTTLRSLKDALFKGAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEV VVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAH	515

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

USP8_pLDDT.png

TSPAN3_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
USP8
TSPAN3

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to USP8-TSPAN3

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to USP8-TSPAN3

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source