UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level1

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:BLNK-ZNF536

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: BLNK-ZNF536
	FusionPDB ID: 9815
	FusionGDB2.0 ID: 9815
		Hgene	Tgene
	Gene symbol	BLNK	ZNF536
	Gene ID	29760	9745
	Gene name	B cell linker	zinc finger protein 536
	Synonyms	AGM4\|BASH\|BLNK-S\|LY57\|SLP-65\|SLP65\|bca	-
	Cytomap	10q24.1	19q12
	Type of gene	protein-coding	protein-coding
	Description	B-cell linker proteinB cell adaptor containing SH2 domainB-cell activationB-cell adapter containing a SH2 domain proteinB-cell adapter containing a Src homology 2 domain proteinSrc homology 2 domain-containing leukocyte protein of 65 kDaSrc homology	zinc finger protein 536
	Modification date	20200313	20200313
	UniProtAcc	Q8WV28	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000224337, ENST00000371176, ENST00000413476, ENST00000427367, ENST00000495266,	ENST00000590564, ENST00000355537,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	7 X 6 X 6=252	21 X 13 X 13=3549
	# samples	7	25
	** MAII score	log2(7/252*10)=-1.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(25/3549*10)=-3.82741257439886 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: BLNK [Title/Abstract] AND ZNF536 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	BLNK(98002491)-ZNF536(31025754), # samples:3
Anticipated loss of major functional domain due to fusion event.	BLNK-ZNF536 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BLNK-ZNF536 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BLNK-ZNF536 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. BLNK-ZNF536 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	BLNK	GO:0035556	intracellular signal transduction	9341187
Tgene	ZNF536	GO:0000122	negative regulation of transcription by RNA polymerase II	19398580

Fusion gene breakpoints across BLNK (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ZNF536 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-A8-A08I-01A	BLNK	chr10	98002491	-	ZNF536	chr19	31025754	+

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000224337	BLNK	chr10	98002491	-	ENST00000355537	ZNF536	chr19	31025754	+	2933	305	103	2037	644
ENST00000371176	BLNK	chr10	98002491	-	ENST00000355537	ZNF536	chr19	31025754	+	2933	305	103	2037	644
ENST00000427367	BLNK	chr10	98002491	-	ENST00000355537	ZNF536	chr19	31025754	+	2969	341	139	2073	644
ENST00000413476	BLNK	chr10	98002491	-	ENST00000355537	ZNF536	chr19	31025754	+	2969	341	139	2073	644
ENST00000495266	BLNK	chr10	98002491	-	ENST00000355537	ZNF536	chr19	31025754	+	2791	163	0	1895	631

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000224337	ENST00000355537	BLNK	chr10	98002491	-	ZNF536	chr19	31025754	+	0.000517995	0.999482
ENST00000371176	ENST00000355537	BLNK	chr10	98002491	-	ZNF536	chr19	31025754	+	0.000517995	0.999482
ENST00000427367	ENST00000355537	BLNK	chr10	98002491	-	ZNF536	chr19	31025754	+	0.000529825	0.9994702
ENST00000413476	ENST00000355537	BLNK	chr10	98002491	-	ZNF536	chr19	31025754	+	0.000529825	0.9994702
ENST00000495266	ENST00000355537	BLNK	chr10	98002491	-	ZNF536	chr19	31025754	+	0.00049188	0.99950814

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>9815_9815_1_BLNK-ZNF536_BLNK_chr10_98002491_ENST00000224337_ZNF536_chr19_31025754_ENST00000355537_length(amino acids)=644AA_BP=65
MDSYSCLLQLPNRMDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASDIGEEAGRSAGVQQPALLRDRSL
GSAMKDCPYCGKTFRTSHHLKVHLRIHTGEKPYKCPHCDYAGTQSASLKYHLERHHRERQNGAGPLSGQPPNQDHKDEMSSKASLFIRPD
ILRGAFKGLPGIDFRGGPASQQWTSGVLSSGDHSGQATGMSSEVPSDALKGTDLPSKSTHFSEIGRAYQSIVSNGVNFQGSLQAFMDSFV
LSSLKKEKDMKDKALADPPSMKVHGVDGGEEKPSGKSSQRKSEKSQYEPLDLSVRPDAASLPGSSVTVQDSIAWHGCLFCAFTTSSMELM
ALHLQANHLGKAKRKDNTIGVTVNCKDQAREASKMALLPSLQSNKDLGLSNMISSLDSASEKMAQGQLKETLGEQKSGAWTGHVDPAFCN
FPSDFYKQFGVYPGMVGSGASSSCPNKEPDGKAHSEEDVPILIPETTSKNTTDDLSDIASSEDMDSSKGENNDEEDVETEPEMMTKPLSA
LSKDSSSDGGDSLQPTGTSQPVQGLVSPLSQAPEKQWHSQGLLQAQDPLAGLPKPERGPQSLDKPMNMLSVLRAYSSDGLAAFNGLASST

--------------------------------------------------------------

>9815_9815_2_BLNK-ZNF536_BLNK_chr10_98002491_ENST00000371176_ZNF536_chr19_31025754_ENST00000355537_length(amino acids)=644AA_BP=65
MDSYSCLLQLPNRMDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASDIGEEAGRSAGVQQPALLRDRSL
GSAMKDCPYCGKTFRTSHHLKVHLRIHTGEKPYKCPHCDYAGTQSASLKYHLERHHRERQNGAGPLSGQPPNQDHKDEMSSKASLFIRPD
ILRGAFKGLPGIDFRGGPASQQWTSGVLSSGDHSGQATGMSSEVPSDALKGTDLPSKSTHFSEIGRAYQSIVSNGVNFQGSLQAFMDSFV
LSSLKKEKDMKDKALADPPSMKVHGVDGGEEKPSGKSSQRKSEKSQYEPLDLSVRPDAASLPGSSVTVQDSIAWHGCLFCAFTTSSMELM
ALHLQANHLGKAKRKDNTIGVTVNCKDQAREASKMALLPSLQSNKDLGLSNMISSLDSASEKMAQGQLKETLGEQKSGAWTGHVDPAFCN
FPSDFYKQFGVYPGMVGSGASSSCPNKEPDGKAHSEEDVPILIPETTSKNTTDDLSDIASSEDMDSSKGENNDEEDVETEPEMMTKPLSA
LSKDSSSDGGDSLQPTGTSQPVQGLVSPLSQAPEKQWHSQGLLQAQDPLAGLPKPERGPQSLDKPMNMLSVLRAYSSDGLAAFNGLASST

--------------------------------------------------------------

>9815_9815_3_BLNK-ZNF536_BLNK_chr10_98002491_ENST00000413476_ZNF536_chr19_31025754_ENST00000355537_length(amino acids)=644AA_BP=65
MDSYSCLLQLPNRMDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASDIGEEAGRSAGVQQPALLRDRSL
GSAMKDCPYCGKTFRTSHHLKVHLRIHTGEKPYKCPHCDYAGTQSASLKYHLERHHRERQNGAGPLSGQPPNQDHKDEMSSKASLFIRPD
ILRGAFKGLPGIDFRGGPASQQWTSGVLSSGDHSGQATGMSSEVPSDALKGTDLPSKSTHFSEIGRAYQSIVSNGVNFQGSLQAFMDSFV
LSSLKKEKDMKDKALADPPSMKVHGVDGGEEKPSGKSSQRKSEKSQYEPLDLSVRPDAASLPGSSVTVQDSIAWHGCLFCAFTTSSMELM
ALHLQANHLGKAKRKDNTIGVTVNCKDQAREASKMALLPSLQSNKDLGLSNMISSLDSASEKMAQGQLKETLGEQKSGAWTGHVDPAFCN
FPSDFYKQFGVYPGMVGSGASSSCPNKEPDGKAHSEEDVPILIPETTSKNTTDDLSDIASSEDMDSSKGENNDEEDVETEPEMMTKPLSA
LSKDSSSDGGDSLQPTGTSQPVQGLVSPLSQAPEKQWHSQGLLQAQDPLAGLPKPERGPQSLDKPMNMLSVLRAYSSDGLAAFNGLASST

--------------------------------------------------------------

>9815_9815_4_BLNK-ZNF536_BLNK_chr10_98002491_ENST00000427367_ZNF536_chr19_31025754_ENST00000355537_length(amino acids)=644AA_BP=65
MDSYSCLLQLPNRMDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASDIGEEAGRSAGVQQPALLRDRSL
GSAMKDCPYCGKTFRTSHHLKVHLRIHTGEKPYKCPHCDYAGTQSASLKYHLERHHRERQNGAGPLSGQPPNQDHKDEMSSKASLFIRPD
ILRGAFKGLPGIDFRGGPASQQWTSGVLSSGDHSGQATGMSSEVPSDALKGTDLPSKSTHFSEIGRAYQSIVSNGVNFQGSLQAFMDSFV
LSSLKKEKDMKDKALADPPSMKVHGVDGGEEKPSGKSSQRKSEKSQYEPLDLSVRPDAASLPGSSVTVQDSIAWHGCLFCAFTTSSMELM
ALHLQANHLGKAKRKDNTIGVTVNCKDQAREASKMALLPSLQSNKDLGLSNMISSLDSASEKMAQGQLKETLGEQKSGAWTGHVDPAFCN
FPSDFYKQFGVYPGMVGSGASSSCPNKEPDGKAHSEEDVPILIPETTSKNTTDDLSDIASSEDMDSSKGENNDEEDVETEPEMMTKPLSA
LSKDSSSDGGDSLQPTGTSQPVQGLVSPLSQAPEKQWHSQGLLQAQDPLAGLPKPERGPQSLDKPMNMLSVLRAYSSDGLAAFNGLASST

--------------------------------------------------------------

>9815_9815_5_BLNK-ZNF536_BLNK_chr10_98002491_ENST00000495266_ZNF536_chr19_31025754_ENST00000355537_length(amino acids)=631AA_BP=52
MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASDIGEEAGRSAGVQQPALLRDRSLGSAMKDCPYCGKT
FRTSHHLKVHLRIHTGEKPYKCPHCDYAGTQSASLKYHLERHHRERQNGAGPLSGQPPNQDHKDEMSSKASLFIRPDILRGAFKGLPGID
FRGGPASQQWTSGVLSSGDHSGQATGMSSEVPSDALKGTDLPSKSTHFSEIGRAYQSIVSNGVNFQGSLQAFMDSFVLSSLKKEKDMKDK
ALADPPSMKVHGVDGGEEKPSGKSSQRKSEKSQYEPLDLSVRPDAASLPGSSVTVQDSIAWHGCLFCAFTTSSMELMALHLQANHLGKAK
RKDNTIGVTVNCKDQAREASKMALLPSLQSNKDLGLSNMISSLDSASEKMAQGQLKETLGEQKSGAWTGHVDPAFCNFPSDFYKQFGVYP
GMVGSGASSSCPNKEPDGKAHSEEDVPILIPETTSKNTTDDLSDIASSEDMDSSKGENNDEEDVETEPEMMTKPLSALSKDSSSDGGDSL
QPTGTSQPVQGLVSPLSQAPEKQWHSQGLLQAQDPLAGLPKPERGPQSLDKPMNMLSVLRAYSSDGLAAFNGLASSTANSGCIKRPDLCG

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:98002491/chr19:31025754)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
BLNK Q8WV28	.
FUNCTION: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis. {ECO:0000269\|PubMed:10583958, ECO:0000269\|PubMed:15270728, ECO:0000269\|PubMed:16912232, ECO:0000269\|PubMed:9697839}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

751_773

723.3333333333334

1301.0

Zinc finger

C2H2-type 8

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

779_801

723.3333333333334

1301.0

Zinc finger

C2H2-type 9

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

BLNK

chr10:98002491

chr19:31025754

ENST00000224337

98_260

54.333333333333336

457.0

Compositional bias

Note=Pro-rich

Hgene

BLNK

chr10:98002491

chr19:31025754

ENST00000371176

98_260

54.333333333333336

434.0

Compositional bias

Note=Pro-rich

Hgene

BLNK

chr10:98002491

chr19:31025754

ENST00000413476

98_260

54.333333333333336

405.0

Compositional bias

Note=Pro-rich

Hgene

BLNK

chr10:98002491

chr19:31025754

ENST00000224337

346_453

54.333333333333336

457.0

Domain

SH2

Hgene

BLNK

chr10:98002491

chr19:31025754

ENST00000371176

346_453

54.333333333333336

434.0

Domain

SH2

Hgene

BLNK

chr10:98002491

chr19:31025754

ENST00000413476

346_453

54.333333333333336

405.0

Domain

SH2

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

130_152

723.3333333333334

1301.0

Zinc finger

C2H2-type 1

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

158_180

723.3333333333334

1301.0

Zinc finger

C2H2-type 2

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

274_297

723.3333333333334

1301.0

Zinc finger

C2H2-type 3

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

300_323

723.3333333333334

1301.0

Zinc finger

C2H2-type 4

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

345_367

723.3333333333334

1301.0

Zinc finger

C2H2-type 5

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

373_395

723.3333333333334

1301.0

Zinc finger

C2H2-type 6

Tgene

ZNF536

chr10:98002491

chr19:31025754

ENST00000355537

631_653

723.3333333333334

1301.0

Zinc finger

C2H2-type 7

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
BLNK
ZNF536

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to BLNK-ZNF536

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to BLNK-ZNF536

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source