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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ZNF98-HSPA8 (FusionGDB2 ID:103117)

Fusion Gene Summary for ZNF98-HSPA8

check button Fusion gene summary
Fusion gene informationFusion gene name: ZNF98-HSPA8
Fusion gene ID: 103117
HgeneTgene
Gene symbol

ZNF98

HSPA8

Gene ID

148198

3312

Gene namezinc finger protein 98heat shock protein family A (Hsp70) member 8
SynonymsF7175|ZNF739HEL-33|HEL-S-72p|HSC54|HSC70|HSC71|HSP71|HSP73|HSPA10|LAP-1|LAP1|NIP71
Cytomap

19p12

11q24.1

Type of geneprotein-codingprotein-coding
Descriptionzinc finger protein 98zinc finger protein 739zinc finger protein F7175heat shock cognate 71 kDa proteinLPS-associated protein 1N-myristoyltransferase inhibitor protein 71constitutive heat shock protein 70epididymis luminal protein 33epididymis secretory sperm binding protein Li 72pheat shock 70kDa protein 8heat shock
Modification date2020031320200327
UniProtAcc.

P11142

Ensembl transtripts involved in fusion geneENST00000357774, ENST00000599879, 
ENST00000601553, 		ENST00000453788, 
ENST00000532636, ENST00000533540, 
ENST00000534624, ENST00000227378, 
ENST00000526110, ENST00000526862, 
ENST00000534319, 
Fusion gene scores* DoF score5 X 3 X 5=7520 X 20 X 4=1600
# samples 523
** MAII scorelog2(5/75*10)=-0.584962500721156
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(23/1600*10)=-2.79836613883035
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ZNF98 [Title/Abstract] AND HSPA8 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointZNF98(22603961)-HSPA8(122928207), # samples:2
HSPA8(122928205)-ZNF98(22603961), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneHSPA8

GO:0042026

protein refolding

21231916|25719862

TgeneHSPA8

GO:0045892

negative regulation of transcription, DNA-templated

10722728

TgeneHSPA8

GO:0046034

ATP metabolic process

23921388

TgeneHSPA8

GO:1902904

negative regulation of supramolecular fiber organization

23921388


check buttonFusion gene breakpoints across ZNF98 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across HSPA8 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/AAI674465ZNF98chr19

22603961

+HSPA8chr11

122928207

+
ChiTaRS5.0N/ABM987321ZNF98chr19

22603961

+HSPA8chr11

122928207

+


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Fusion Gene ORF analysis for ZNF98-HSPA8

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-3UTRENST00000357774ENST00000453788ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000357774ENST00000532636ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000357774ENST00000533540ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000357774ENST00000534624ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000599879ENST00000453788ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000599879ENST00000532636ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000599879ENST00000533540ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000599879ENST00000534624ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000601553ENST00000453788ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000601553ENST00000532636ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000601553ENST00000533540ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-3UTRENST00000601553ENST00000534624ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000357774ENST00000227378ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000357774ENST00000526110ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000357774ENST00000526862ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000357774ENST00000534319ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000599879ENST00000227378ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000599879ENST00000526110ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000599879ENST00000526862ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000599879ENST00000534319ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000601553ENST00000227378ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000601553ENST00000526110ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000601553ENST00000526862ZNF98chr19

22603961

+HSPA8chr11

122928207

+
intron-intronENST00000601553ENST00000534319ZNF98chr19

22603961

+HSPA8chr11

122928207

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ZNF98-HSPA8


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for ZNF98-HSPA8


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:22603961/:122928207)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.HSPA8

P11142

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation (PubMed:21150129, PubMed:21148293, PubMed:24732912, PubMed:27916661, PubMed:23018488). This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones (PubMed:21150129, PubMed:21148293, PubMed:24732912, PubMed:27916661, PubMed:23018488, PubMed:12526792). The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation (PubMed:21150129, PubMed:21148293, PubMed:24732912, PubMed:27916661, PubMed:23018488, PubMed:12526792). The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. HSP70 goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The HSP70-associated co-chaperones are of three types: J-domain co-chaperones HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1 (PubMed:24318877, PubMed:27474739, PubMed:24121476, PubMed:26865365). Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70 (PubMed:12526792). Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:10722728, PubMed:11276205). Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase STUB1 (PubMed:23990462). Interacts with VGF-derived peptide TLQP-21 (PubMed:28934328). {ECO:0000269|PubMed:10722728, ECO:0000269|PubMed:11276205, ECO:0000269|PubMed:12526792, ECO:0000269|PubMed:21148293, ECO:0000269|PubMed:21150129, ECO:0000269|PubMed:23018488, ECO:0000269|PubMed:23990462, ECO:0000269|PubMed:24318877, ECO:0000269|PubMed:24732912, ECO:0000269|PubMed:27474739, ECO:0000269|PubMed:27916661, ECO:0000269|PubMed:28934328, ECO:0000303|PubMed:24121476, ECO:0000303|PubMed:26865365}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ZNF98-HSPA8


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ZNF98-HSPA8


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ZNF98-HSPA8


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ZNF98-HSPA8


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource