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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:CERS4-HUWE1 (FusionGDB2 ID:15979) |
Fusion Gene Summary for CERS4-HUWE1 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: CERS4-HUWE1 | Fusion gene ID: 15979 | Hgene | Tgene | Gene symbol | CERS4 | HUWE1 | Gene ID | 79603 | 10075 |
| Gene name | ceramide synthase 4 | HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 | |
| Synonyms | LASS4|Trh1 | ARF-BP1|HECTH9|HSPC272|Ib772|LASU1|MRXST|MULE|URE-B1|UREB1 | |
| Cytomap | 19p13.2 | Xp11.22 | |
| Type of gene | protein-coding | protein-coding | |
| Description | ceramide synthase 4LAG1 homolog, ceramide synthase 4LAG1 longevity assurance homolog 4sphingosine N-acyltransferase CERS4 | E3 ubiquitin-protein ligase HUWE1ARF-binding protein 1BJ-HCC-24 tumor antigenHECT domain protein LASU1HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligaseHECT-type E3 ubiquitin transferase HUWE1Mcl-1 ubiquitin ligase E3URE-binding pro | |
| Modification date | 20200320 | 20200313 | |
| UniProtAcc | Q9HA82 | Q7Z6Z7 | |
| Ensembl transtripts involved in fusion gene | ENST00000595722, ENST00000251363, ENST00000558331, ENST00000559336, ENST00000559450, | ENST00000262854, ENST00000342160, ENST00000218328, ENST00000474288, | |
| Fusion gene scores | * DoF score | 5 X 3 X 5=75 | 11 X 14 X 5=770 |
| # samples | 5 | 15 | |
| ** MAII score | log2(5/75*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/770*10)=-2.35989594508638 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: CERS4 [Title/Abstract] AND HUWE1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | CERS4(8321594)-HUWE1(53564657), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | CERS4-HUWE1 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. CERS4-HUWE1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CERS4-HUWE1 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. CERS4-HUWE1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CERS4 | GO:0046513 | ceramide biosynthetic process | 17977534|29632068 |
| Tgene | HUWE1 | GO:0000209 | protein polyubiquitination | 15989957 |
| Tgene | HUWE1 | GO:0006513 | protein monoubiquitination | 19713937 |
| Tgene | HUWE1 | GO:0016574 | histone ubiquitination | 15767685 |
| Tgene | HUWE1 | GO:0031398 | positive regulation of protein ubiquitination | 20534529 |
| Tgene | HUWE1 | GO:0098779 | positive regulation of mitophagy in response to mitochondrial depolarization | 30217973 |
| Fusion gene breakpoints across CERS4 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across HUWE1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | ESCA | TCGA-ZR-A9CJ | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
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Fusion Gene ORF analysis for CERS4-HUWE1 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 3UTR-3CDS | ENST00000595722 | ENST00000262854 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 3UTR-3CDS | ENST00000595722 | ENST00000342160 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 3UTR-intron | ENST00000595722 | ENST00000218328 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 3UTR-intron | ENST00000595722 | ENST00000474288 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000251363 | ENST00000218328 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000251363 | ENST00000474288 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000558331 | ENST00000218328 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000558331 | ENST00000474288 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000559336 | ENST00000218328 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000559336 | ENST00000474288 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000559450 | ENST00000218328 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| 5CDS-intron | ENST00000559450 | ENST00000474288 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000251363 | ENST00000262854 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000251363 | ENST00000342160 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000558331 | ENST00000262854 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000558331 | ENST00000342160 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000559336 | ENST00000262854 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000559336 | ENST00000342160 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000559450 | ENST00000262854 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| Frame-shift | ENST00000559450 | ENST00000342160 | CERS4 | chr19 | 8321594 | + | HUWE1 | chrX | 53564657 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for CERS4-HUWE1 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for CERS4-HUWE1 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:8321594/:53564657) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| CERS4 | HUWE1 |
| FUNCTION: Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward long and very-long chains (C18:0-C22:0) as acyl donor. {ECO:0000269|PubMed:17977534, ECO:0000269|PubMed:23530041, ECO:0000269|PubMed:26887952, ECO:0000269|PubMed:29632068, ECO:0000269|PubMed:31916624}. | FUNCTION: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15989957, PubMed:19713937, PubMed:15567145, PubMed:15767685, PubMed:18488021, PubMed:17567951, PubMed:19037095, PubMed:20534529). Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1 (PubMed:15989957). Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair (PubMed:19713937). Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4 (PubMed:15567145, PubMed:15767685, PubMed:15989956). Ubiquitinates MFN2 to negatively regulate mitochondrial fusion in response to decreased stearoylation of TFRC (PubMed:26214738). Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN (PubMed:18488021). May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (PubMed:17567951). Mediates polyubiquitination of isoform 2 of PA2G4 (PubMed:19037095). Acts in concert with MYCBP2 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1 (PubMed:20534529). {ECO:0000269|PubMed:15567145, ECO:0000269|PubMed:15767685, ECO:0000269|PubMed:15989956, ECO:0000269|PubMed:15989957, ECO:0000269|PubMed:17567951, ECO:0000269|PubMed:18488021, ECO:0000269|PubMed:19037095, ECO:0000269|PubMed:19713937, ECO:0000269|PubMed:20534529, ECO:0000269|PubMed:26214738}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for CERS4-HUWE1 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for CERS4-HUWE1 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for CERS4-HUWE1 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for CERS4-HUWE1 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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