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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:CFLAR-AGFG1 (FusionGDB2 ID:16142)

Fusion Gene Summary for CFLAR-AGFG1

Fusion gene summary

Fusion gene information	Fusion gene name: CFLAR-AGFG1
	Fusion gene ID: 16142
		Hgene	Tgene
	Gene symbol	CFLAR	AGFG1
	Gene ID	8837	3267
	Gene name	CASP8 and FADD like apoptosis regulator	ArfGAP with FG repeats 1
	Synonyms	CASH\|CASP8AP1\|CLARP\|Casper\|FLAME\|FLAME-1\|FLAME1\|FLIP\|I-FLICE\|MRIT\|c-FLIP\|c-FLIPL\|c-FLIPR\|c-FLIPS\|cFLIP	HRB\|RAB\|RIP
	Cytomap	2q33.1	2q36.3
	Type of gene	protein-coding	protein-coding
	Description	CASP8 and FADD-like apoptosis regulatorFADD-like antiapoptotic molecule 1MACH-related inducer of toxicitycaspase homologcaspase-eight-related proteincaspase-like apoptosis regulatory proteincaspase-related inducer of apoptosiscellular FLICE-like in	arf-GAP domain and FG repeat-containing protein 1HIV-1 Rev-binding proteinRab, Rev/Rex activation domain-binding proteinarf-GAP domain and FG repeats-containing protein 1hRIP, Rev interacting proteinnucleoporin-like protein RIPrev-interacting protei
	Modification date	20200322	20200313
	UniProtAcc	O15519	P52594
	Ensembl transtripts involved in fusion gene	ENST00000309955, ENST00000340870, ENST00000341222, ENST00000341582, ENST00000342795, ENST00000355558, ENST00000395148, ENST00000423241, ENST00000440180, ENST00000457277, ENST00000443227, ENST00000479953, ENST00000490965, ENST00000494258,	ENST00000486932, ENST00000310078, ENST00000373671, ENST00000409171, ENST00000409315, ENST00000409979,
Fusion gene scores	* DoF score	9 X 10 X 9=810	10 X 11 X 4=440
	# samples	12	11
	** MAII score	log2(12/810*10)=-2.75488750216347 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(11/440*10)=-2 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: CFLAR [Title/Abstract] AND AGFG1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	CFLAR(201994869)-AGFG1(228418420), # samples:1
Anticipated loss of major functional domain due to fusion event.	CFLAR-AGFG1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. CFLAR-AGFG1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	CFLAR	GO:0060544	regulation of necroptotic process	21737330
Hgene	CFLAR	GO:1902042	negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	26582200
Hgene	CFLAR	GO:2001237	negative regulation of extrinsic apoptotic signaling pathway	9208847

Fusion gene breakpoints across CFLAR (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across AGFG1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	Non-Cancer	ERR315487	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+

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Fusion Gene ORF analysis for CFLAR-AGFG1

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-intron	ENST00000309955	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000340870	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000341222	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000341582	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000342795	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000355558	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000395148	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000423241	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000440180	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
5CDS-intron	ENST00000457277	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000309955	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000309955	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000309955	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000309955	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000309955	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000340870	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000340870	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000340870	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000340870	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000340870	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341222	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341222	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341222	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341222	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341222	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341582	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341582	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341582	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341582	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000341582	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000342795	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000342795	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000342795	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000342795	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000342795	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000355558	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000355558	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000355558	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000355558	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000355558	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000395148	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000395148	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000395148	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000395148	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000395148	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000423241	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000423241	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000423241	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000423241	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000423241	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000440180	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000440180	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000440180	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000440180	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000440180	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000457277	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000457277	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000457277	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000457277	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
Frame-shift	ENST00000457277	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000443227	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000443227	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000443227	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000443227	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000443227	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000479953	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000479953	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000479953	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000479953	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000479953	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000490965	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000490965	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000490965	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000490965	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000490965	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000494258	ENST00000310078	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000494258	ENST00000373671	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000494258	ENST00000409171	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000494258	ENST00000409315	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-3CDS	ENST00000494258	ENST00000409979	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-intron	ENST00000443227	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-intron	ENST00000479953	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-intron	ENST00000490965	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+
intron-intron	ENST00000494258	ENST00000486932	CFLAR	chr2	201994869	+	AGFG1	chr2	228418420	+

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

No-coding score

Coding score

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Fusion Genomic Features for CFLAR-AGFG1

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	1-p	p (fusion gene breakpoint)
CFLAR	chr2	201994869	+	AGFG1	chr2	228418419	+	1.35E-06	0.9999987
CFLAR	chr2	201994869	+	AGFG1	chr2	228418419	+	1.35E-06	0.9999987

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for CFLAR-AGFG1

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:201994869/:228418420)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
CFLAR O15519	AGFG1 P52594
FUNCTION: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. {ECO:0000269\|PubMed:9880531}.	FUNCTION: Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. {ECO:0000250, ECO:0000269\|PubMed:10613896, ECO:0000269\|PubMed:14701878, ECO:0000269\|PubMed:15749819}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

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Fusion Gene Sequence for CFLAR-AGFG1

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for CFLAR-AGFG1

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for CFLAR-AGFG1

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

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Related Diseases for CFLAR-AGFG1

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source