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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:CHMP4A-MPHOSPH8 (FusionGDB2 ID:16547) |
Fusion Gene Summary for CHMP4A-MPHOSPH8 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: CHMP4A-MPHOSPH8 | Fusion gene ID: 16547 | Hgene | Tgene | Gene symbol | CHMP4A | MPHOSPH8 | Gene ID | 128866 | 54737 |
| Gene name | charged multivesicular body protein 4B | M-phase phosphoprotein 8 | |
| Synonyms | C20orf178|CHMP4A|CTPP3|CTRCT31|SNF7|SNF7-2|Shax1|VPS32B|Vps32-2|dJ553F4.4 | HSMPP8|TWA3|mpp8 | |
| Cytomap | 20q11.22 | 13q12.11 | |
| Type of gene | protein-coding | protein-coding | |
| Description | charged multivesicular body protein 4bSNF7 homolog associated with Alix 1Snf7 homologue associated with Alix 1chromatin modifying protein 4Bchromatin-modifying protein 4bhSnf7-2hVps32-2vacuolar protein-sorting-associated protein 32-2 | M-phase phosphoprotein 8M-phase phosphoprotein, mppM-phase phosphoprotein, mpp8two hybrid-associated protein 3 with RanBPM | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q9BY43 | Q99549 | |
| Ensembl transtripts involved in fusion gene | ENST00000347519, ENST00000609024, ENST00000530996, ENST00000542700, | ENST00000496525, ENST00000361479, ENST00000414242, | |
| Fusion gene scores | * DoF score | 4 X 3 X 2=24 | 25 X 17 X 10=4250 |
| # samples | 4 | 30 | |
| ** MAII score | log2(4/24*10)=0.736965594166206 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(30/4250*10)=-3.82442843541655 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: CHMP4A [Title/Abstract] AND MPHOSPH8 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | CHMP4A(24682614)-MPHOSPH8(20245345), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CHMP4A | GO:0039702 | viral budding via host ESCRT complex | 24878737 |
| Hgene | CHMP4A | GO:0051258 | protein polymerization | 18209100 |
| Tgene | MPHOSPH8 | GO:0045814 | negative regulation of gene expression, epigenetic | 26022416|28581500|29211708 |
| Tgene | MPHOSPH8 | GO:0090309 | positive regulation of methylation-dependent chromatin silencing | 28581500|29211708 |
| Fusion gene breakpoints across CHMP4A (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across MPHOSPH8 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | OV | TCGA-09-1668 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
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Fusion Gene ORF analysis for CHMP4A-MPHOSPH8 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-3UTR | ENST00000347519 | ENST00000496525 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5CDS-3UTR | ENST00000609024 | ENST00000496525 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5CDS-intron | ENST00000347519 | ENST00000361479 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5CDS-intron | ENST00000347519 | ENST00000414242 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5CDS-intron | ENST00000609024 | ENST00000361479 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5CDS-intron | ENST00000609024 | ENST00000414242 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5UTR-3UTR | ENST00000530996 | ENST00000496525 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5UTR-intron | ENST00000530996 | ENST00000361479 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| 5UTR-intron | ENST00000530996 | ENST00000414242 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| intron-3UTR | ENST00000542700 | ENST00000496525 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| intron-intron | ENST00000542700 | ENST00000361479 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| intron-intron | ENST00000542700 | ENST00000414242 | CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for CHMP4A-MPHOSPH8 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + | 0.3789414 | 0.62105864 |
| CHMP4A | chr14 | 24682614 | - | MPHOSPH8 | chr13 | 20245345 | + | 0.3789414 | 0.62105864 |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
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| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for CHMP4A-MPHOSPH8 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:24682614/:20245345) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| CHMP4A | MPHOSPH8 |
| FUNCTION: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:12860994, ECO:0000269|PubMed:14505569, ECO:0000269|PubMed:14519844, ECO:0000269|PubMed:14583093, ECO:0000269|PubMed:18209100, ECO:0000269|PubMed:22660413}. | FUNCTION: Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:26022416, ECO:0000269|PubMed:28581500, ECO:0000269|PubMed:29211708, ECO:0000269|PubMed:30487602}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for CHMP4A-MPHOSPH8 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for CHMP4A-MPHOSPH8 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for CHMP4A-MPHOSPH8 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for CHMP4A-MPHOSPH8 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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