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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CUL3-SPAG16 (FusionGDB2 ID:20673)

Fusion Gene Summary for CUL3-SPAG16

check button Fusion gene summary
Fusion gene informationFusion gene name: CUL3-SPAG16
Fusion gene ID: 20673
HgeneTgene
Gene symbol

CUL3

SPAG16

Gene ID

8452

79582

Gene namecullin 3sperm associated antigen 16
SynonymsCUL-3|PHA2EPF20|WDR29
Cytomap

2q36.2

2q34

Type of geneprotein-codingprotein-coding
Descriptioncullin-3sperm-associated antigen 16 proteinWD repeat domain 29pf20 protein homologsperm-associated WD repeat protein
Modification date2020032720200313
UniProtAcc

Q13618

.
Ensembl transtripts involved in fusion geneENST00000264414, ENST00000344951, 
ENST00000409096, ENST00000409777, 
ENST00000432260, 		ENST00000414961, 
ENST00000272898, ENST00000374309, 
ENST00000413312, ENST00000432529, 
ENST00000447990, ENST00000331683, 
Fusion gene scores* DoF score12 X 12 X 7=100816 X 16 X 8=2048
# samples 1418
** MAII scorelog2(14/1008*10)=-2.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(18/2048*10)=-3.50814690367033
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: CUL3 [Title/Abstract] AND SPAG16 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointCUL3(225360549)-SPAG16(214174783), # samples:3
SPAG16(214162081)-CUL3(225346795), # samples:3
Anticipated loss of major functional domain due to fusion event.CUL3-SPAG16 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
CUL3-SPAG16 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF.
SPAG16-CUL3 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
SPAG16-CUL3 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneCUL3

GO:0000209

protein polyubiquitination

19261606

HgeneCUL3

GO:0006511

ubiquitin-dependent protein catabolic process

25401743|27561354

HgeneCUL3

GO:0006513

protein monoubiquitination

22358839

HgeneCUL3

GO:0006888

ER to Golgi vesicle-mediated transport

22358839

HgeneCUL3

GO:0016567

protein ubiquitination

17543862|19782033|19995937|20389280|23213400

HgeneCUL3

GO:0031145

anaphase-promoting complex-dependent catabolic process

10500095

HgeneCUL3

GO:0043161

proteasome-mediated ubiquitin-dependent protein catabolic process

19261606|19782033|20389280

HgeneCUL3

GO:0071630

nuclear protein quality control by the ubiquitin-proteasome system

27561354


check buttonFusion gene breakpoints across CUL3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across SPAG16 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUSCTCGA-O2-A52V-01ACUL3chr2

225360549

-SPAG16chr2

214174783

+
ChimerDB4LUSCTCGA-O2-A52VCUL3chr2

225360549

-SPAG16chr2

214174783

+
ChimerDB4LUSCTCGA-O2-A52VCUL3chr2

225360549

-SPAG16chr2

214181943

+


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Fusion Gene ORF analysis for CUL3-SPAG16

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000264414ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-3UTRENST00000264414ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214181943

+
5CDS-3UTRENST00000344951ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-3UTRENST00000344951ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214181943

+
5CDS-3UTRENST00000409096ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-3UTRENST00000409096ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214181943

+
5CDS-3UTRENST00000409777ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-3UTRENST00000409777ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214181943

+
5CDS-intronENST00000264414ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000264414ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000264414ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000264414ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000264414ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000344951ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000344951ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000344951ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000344951ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000344951ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409096ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409096ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409096ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409096ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409096ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409777ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409777ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409777ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409777ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214174783

+
5CDS-intronENST00000409777ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214174783

+
Frame-shiftENST00000264414ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000264414ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000264414ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000264414ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000264414ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000344951ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000344951ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000344951ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000344951ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000344951ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000409096ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000409096ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000409096ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000409096ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000409096ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214181943

+
Frame-shiftENST00000409777ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214174783

+
Frame-shiftENST00000409777ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000264414ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214174783

+
In-frameENST00000264414ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000344951ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214174783

+
In-frameENST00000344951ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000409096ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214174783

+
In-frameENST00000409096ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000409777ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000409777ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000409777ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000409777ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214181943

+
In-frameENST00000409777ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3CDSENST00000432260ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3CDSENST00000432260ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214174783

+
intron-3CDSENST00000432260ENST00000331683CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3CDSENST00000432260ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3CDSENST00000432260ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3CDSENST00000432260ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3CDSENST00000432260ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-3UTRENST00000432260ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214174783

+
intron-3UTRENST00000432260ENST00000414961CUL3chr2

225360549

-SPAG16chr2

214181943

+
intron-intronENST00000432260ENST00000272898CUL3chr2

225360549

-SPAG16chr2

214174783

+
intron-intronENST00000432260ENST00000374309CUL3chr2

225360549

-SPAG16chr2

214174783

+
intron-intronENST00000432260ENST00000413312CUL3chr2

225360549

-SPAG16chr2

214174783

+
intron-intronENST00000432260ENST00000432529CUL3chr2

225360549

-SPAG16chr2

214174783

+
intron-intronENST00000432260ENST00000447990CUL3chr2

225360549

-SPAG16chr2

214174783

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000264414CUL3chr2225360549-ENST00000331683SPAG16chr2214174783+3984218133937971152
ENST00000344951CUL3chr2225360549-ENST00000331683SPAG16chr2214174783+3831202838436441086
ENST00000409096CUL3chr2225360549-ENST00000331683SPAG16chr2214174783+371619135335291158
ENST00000264414CUL3chr2225360549-ENST00000432529SPAG16chr2214181943+294121813392186615
ENST00000344951CUL3chr2225360549-ENST00000432529SPAG16chr2214181943+278820283842033549
ENST00000409096CUL3chr2225360549-ENST00000432529SPAG16chr2214181943+26731913531918621
ENST00000409777CUL3chr2225360549-ENST00000331683SPAG16chr2214181943+406023766002381593
ENST00000409777CUL3chr2225360549-ENST00000374309SPAG16chr2214181943+387423766002381593
ENST00000409777CUL3chr2225360549-ENST00000413312SPAG16chr2214181943+408123766002381593
ENST00000409777CUL3chr2225360549-ENST00000272898SPAG16chr2214181943+302223766002381593
ENST00000409777CUL3chr2225360549-ENST00000447990SPAG16chr2214181943+330523766002381593

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000264414ENST00000331683CUL3chr2225360549-SPAG16chr2214174783+0.0003569050.9996431
ENST00000344951ENST00000331683CUL3chr2225360549-SPAG16chr2214174783+0.000428270.99957174
ENST00000409096ENST00000331683CUL3chr2225360549-SPAG16chr2214174783+0.0002065910.99979347
ENST00000264414ENST00000432529CUL3chr2225360549-SPAG16chr2214181943+0.0005867170.99941325
ENST00000344951ENST00000432529CUL3chr2225360549-SPAG16chr2214181943+0.0008114170.9991886
ENST00000409096ENST00000432529CUL3chr2225360549-SPAG16chr2214181943+0.0003249460.99967504
ENST00000409777ENST00000331683CUL3chr2225360549-SPAG16chr2214181943+0.0002627630.9997372
ENST00000409777ENST00000374309CUL3chr2225360549-SPAG16chr2214181943+0.0002704840.99972945
ENST00000409777ENST00000413312CUL3chr2225360549-SPAG16chr2214181943+0.0002545820.9997454
ENST00000409777ENST00000272898CUL3chr2225360549-SPAG16chr2214181943+0.0003848680.9996151
ENST00000409777ENST00000447990CUL3chr2225360549-SPAG16chr2214181943+0.0003865440.99961346

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Fusion Genomic Features for CUL3-SPAG16


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
CUL3chr2225360548-SPAG16chr2214181942+0.0081237760.99187624
CUL3chr2225360548-SPAG16chr2214174782+0.0010672920.99893266
CUL3chr2225360548-SPAG16chr2214174782+0.0010672920.99893266
CUL3chr2225360548-SPAG16chr2214181942+0.0081237760.99187624
CUL3chr2225360548-SPAG16chr2214174782+0.0010672920.99893266
CUL3chr2225360548-SPAG16chr2214174782+0.0010672920.99893266

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for CUL3-SPAG16


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:225360549/chr2:214174783)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
CUL3

Q13618

.
FUNCTION: Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. BCR(KLHL42) is involved in ubiquitination of KATNA1. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, BRMS1, MACROH2A1 and DAXX, GLI2 and GLI3. Can also form a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL; these complexes have lower ubiquitin ligase activity. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27716508). BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; by mediating ubiquitination of WNK4 (PubMed:23387299, PubMed:23453970, PubMed:23576762). The BCR(KLHL20) E3 ubiquitin ligase complex is involved in interferon response and anterograde Golgi to endosome transport: it mediates both ubiquitination leading to degradation and 'Lys-33'-linked ubiquitination (PubMed:20389280, PubMed:21840486, PubMed:21670212, PubMed:24768539). The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB (PubMed:19995937). The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation (PubMed:23455478). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway (PubMed:29769719). The BCR(KLHL25) ubiquitin ligase complex is involved in translational homeostasis by mediating ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) (PubMed:22578813). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41 (PubMed:15983046). In concert with ATF2 and RBX1, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. The BCR(KCTD17) E3 ubiquitin ligase complex mediates ubiquitination and degradation of TCHP, a down-regulator of cilium assembly, thereby inducing ciliogenesis (PubMed:25270598). The BCR(KLHL24) E3 ubiquitin ligase complex mediates ubiquitination of KRT14, controls KRT14 levels during keratinocytes differentiation, and is essential for skin integrity (PubMed:27798626). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (PubMed:23213400). The BCR(KEAP1) E3 ubiquitin ligase complex acts as a key sensor of oxidative and electrophilic stress by mediating ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, PubMed:16006525). {ECO:0000269|PubMed:10500095, ECO:0000269|PubMed:11311237, ECO:0000269|PubMed:15601839, ECO:0000269|PubMed:15897469, ECO:0000269|PubMed:15983046, ECO:0000269|PubMed:16006525, ECO:0000269|PubMed:16524876, ECO:0000269|PubMed:17543862, ECO:0000269|PubMed:18397884, ECO:0000269|PubMed:19261606, ECO:0000269|PubMed:19995937, ECO:0000269|PubMed:20389280, ECO:0000269|PubMed:21670212, ECO:0000269|PubMed:21840486, ECO:0000269|PubMed:22085717, ECO:0000269|PubMed:22358839, ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:22632832, ECO:0000269|PubMed:23213400, ECO:0000269|PubMed:23387299, ECO:0000269|PubMed:23453970, ECO:0000269|PubMed:23455478, ECO:0000269|PubMed:23576762, ECO:0000269|PubMed:24768539, ECO:0000269|PubMed:25270598, ECO:0000269|PubMed:26399832, ECO:0000269|PubMed:27565346, ECO:0000269|PubMed:27716508, ECO:0000269|PubMed:27798626, ECO:0000269|PubMed:29769719}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

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* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210152_26793348.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216152_26793632.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217152_26762252.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925152_26793184.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310152_267132348.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316152_267132632.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227152_267101252.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935152_267132184.0Coiled coilOntology_term=ECO:0000255
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210350_38993348.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210392_43193348.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210434_47393348.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210476_51593348.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210518_55793348.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210560_60093348.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214174783ENST00000272898210601_63093348.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216350_38993632.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216392_43193632.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216434_47393632.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216476_51593632.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216518_55793632.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216560_60093632.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214174783ENST00000331683216601_63093632.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217350_38962252.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217392_43162252.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217434_47362252.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217476_51562252.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217518_55762252.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217560_60062252.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214174783ENST0000041331217601_63062252.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925350_38993184.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925392_43193184.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925434_47393184.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925476_51593184.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925518_55793184.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925560_60093184.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214174783ENST0000043252925601_63093184.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310350_389132348.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310392_431132348.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310434_473132348.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310476_515132348.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310518_557132348.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310560_600132348.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214181943ENST00000272898310601_630132348.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316350_389132632.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316392_431132632.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316434_473132632.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316476_515132632.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316518_557132632.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316560_600132632.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214181943ENST00000331683316601_630132632.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227350_389101252.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227392_431101252.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227434_473101252.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227476_515101252.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227518_557101252.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227560_600101252.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214181943ENST0000041331227601_630101252.0RepeatNote=WD 7
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935350_389132184.0RepeatNote=WD 1
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935392_431132184.0RepeatNote=WD 2
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935434_473132184.0RepeatNote=WD 3
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935476_515132184.0RepeatNote=WD 4
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935518_557132184.0RepeatNote=WD 5
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935560_600132184.0RepeatNote=WD 6
TgeneSPAG16chr2:225360549chr2:214181943ENST0000043252935601_630132184.0RepeatNote=WD 7

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for CUL3-SPAG16


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>20673_20673_1_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000264414_SPAG16_chr2_214174783_ENST00000331683_length(transcript)=3984nt_BP=2181nt
CCTGCGCAGTGAGATGTTTGTCCGTCGCCGCCGCCGCCGCCATCGCGGAGGAGCGCGATAAAGGGAGCCGAGCCGGACGTGAGGGGGACC
CCGCGGAGCCGCCGCGCCAGCGCAGCCCCCCAGCCGCATCGGAGTCGCCAGAGTCCGAGCCGCCGCCGCCGCCGCCGCCGCCCCCGCCGC
CGCCGCGGCTGCCCAGGGGCCGGCCAGCTCCCCAGCCCTGCCCGGGGGTCGCGCCGCCGCCGCCCCCAGCGTCGCCCCTAGCCTGCCTGC
CCGCCTTAAATGTGACACCGGCGTCGCGGAGCGCGACCTGAAGCCGCCGCCGGGGAGGGGACGAGCACCATGTCGAATCTGAGCAAAGGC
ACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCGATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTG
AAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCAT
AAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTG
AATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGGAC
CGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATT
AGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTTGC
CAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTT
CAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGA
GTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTA
GAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGCCA
AATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAAT
CCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTAAA
CAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAA
AAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATGTA
TTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATCTAAG
TTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAATTC
AGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCA
GCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGACAG
CTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTT
GGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATAAT
AGAGAAAAATACACATTTGAGGAACGGAAAACAGTTCTTCCTTCAAAGCATGCAGTACCTGAAGTAATAGAAGACTTTCTCTGCAATTTC
TTGATCAAAATGGGAATGACCAGAACTCTTGATTGCTTTCAGTCTGAATGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACT
GTTGGGAATGTTCCAGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAA
GCAGCTGACAAAGCTAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAAAA
AACAAATTAATTAATGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACACT
TTACTGAAGGAGAAAATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGCAA
AGAGGACATAGTTACCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCACCAGAAGGTCCTACTCAGAAAGGTCTT
CGTGAAGCCAGGGAACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGGATTCAGAATTTCCCATAGATATGCAACCAAATCCA
AACCTGAATGTTTCTAAAGAAAGTCTTTCTCCAGCAAAATTTGACTACAAGCTGAAAAACATTTTTAGACTCCATGAACTTCCAGTGAGC
TGTGTCTCCATGCAACCCCACAAAGACATCCTAGTCTCCTGTGGCGAGGACCGACTCTGGAAGGTGTTGGGCCTTCCAAAATGCAATGTG
CTTCTCACGGGATTTGGCCACACTGACTGGCTTTCAGACTGCTGCTTCCATCCCAGTGGCGACAAATTGGCTACTTCAAGTGGTGACACT
ACAGTTAAATTATGGGATCTATGTAAAGGCGATTGCATTTTGACCTTTGAAGGACACAGCCGCGCAGTGTGGTCCTGCACATGGCACTCC
TGCGGCAATTTTGTGGCTTCCTCCTCACTGGATAAAACTAGCAAAATTTGGGATGTTAATAGTGAAAGATGCAGATGTACTTTGTATGGA
CATACAGATTCTGTGAACAGCATTGAGTTTTTTCCTTTCTCCAATACTCTTCTCACAAGCTCTGCAGACAAGACCCTGTCTATATGGGAT
GCAAGAACAGGTATATGTGAGCAGTCACTTTATGGTCACATGCATTCTATCAATGATGCCATTTTTGATCCCAGGGGTCACATGATAGCA
TCCTGTGATGCCTGTGGGGTTACAAAGCTGTGGGACTTTCGGAAGCTGTTACCAATTGTGTCCATCGATATAGGTCCAAGTCCTGGCAAT
GAGGTGAATTTTGATTCATCAGGTCGAGTTTTAGCTCAGGCAAGTGGCAATGGTGTTATCCATTTGCTAGATCTTAAATCTGGGGAGATT
CACAAATTGATGGGCCACGAAAACGAGGCACACACGGTTGTGTTTTCTCACGACGGGGAGATTCTCTTTTCTGGAGGCTCTGACGGCACA
GTTCGAACGTGGTCTTGACCGTCAGCACATCCCGCTGCAGAGGGCATTCCCTTTAAGGCTTGAAAATGCCTCCTGTAGTCCCAAACCAGC
AAAGAACTGGTTAAATGTGCCAGCAGGTAACATTTACAGTCTGCTTTAAAACATGCTTTGGACATATATTTTAGTGCTCATACTGTTACT

>20673_20673_1_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000264414_SPAG16_chr2_214174783_ENST00000331683_length(amino acids)=1152AA_BP=612
MSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVR
EDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDR
GAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELIS
KHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESF
NNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDS
EKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYL
AKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEERKTVLPSKHAVPEVI
EDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAADKAREDLLKIQKERDFHRMHH
KRIVQEKNKLINDLKGLKLHYASYEPTIRVLHEKHHTLLKEKMLTSLERDKVVGQISGLQETLKKLQRGHSYHGPQIKVDHSREKENAPE
GPTQKGLREAREQNKCKTKMKGNTKDSEFPIDMQPNPNLNVSKESLSPAKFDYKLKNIFRLHELPVSCVSMQPHKDILVSCGEDRLWKVL
GLPKCNVLLTGFGHTDWLSDCCFHPSGDKLATSSGDTTVKLWDLCKGDCILTFEGHSRAVWSCTWHSCGNFVASSSLDKTSKIWDVNSER
CRCTLYGHTDSVNSIEFFPFSNTLLTSSADKTLSIWDARTGICEQSLYGHMHSINDAIFDPRGHMIASCDACGVTKLWDFRKLLPIVSID

--------------------------------------------------------------
>20673_20673_2_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000264414_SPAG16_chr2_214181943_ENST00000432529_length(transcript)=2941nt_BP=2181nt
CCTGCGCAGTGAGATGTTTGTCCGTCGCCGCCGCCGCCGCCATCGCGGAGGAGCGCGATAAAGGGAGCCGAGCCGGACGTGAGGGGGACC
CCGCGGAGCCGCCGCGCCAGCGCAGCCCCCCAGCCGCATCGGAGTCGCCAGAGTCCGAGCCGCCGCCGCCGCCGCCGCCGCCCCCGCCGC
CGCCGCGGCTGCCCAGGGGCCGGCCAGCTCCCCAGCCCTGCCCGGGGGTCGCGCCGCCGCCGCCCCCAGCGTCGCCCCTAGCCTGCCTGC
CCGCCTTAAATGTGACACCGGCGTCGCGGAGCGCGACCTGAAGCCGCCGCCGGGGAGGGGACGAGCACCATGTCGAATCTGAGCAAAGGC
ACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCGATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTG
AAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCAT
AAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTG
AATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGGAC
CGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATT
AGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTTGC
CAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTT
CAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGA
GTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTA
GAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGCCA
AATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAAT
CCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTAAA
CAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAA
AAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATGTA
TTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATCTAAG
TTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAATTC
AGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCA
GCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGACAG
CTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTT
GGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATAAT
AGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCCAGATGTCTACACCCA
GATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGAGTATGTTATTTTTTAAATGAC
ATTTTCTTCTTTTTCTTTTGGACTAAATAAAAGAGTTGAGTGAAGCTGATATATGTAATATACCAGAGCCTTAATTTTTGAAAACTGAAT
TTTTCTAGTTGTAAAGAATGTGAGAGGCTTCATTAGCAAATTAATTAAACAGATGATCAGAACTATCACAATTATAACTTACCAACAAGA
AGGGAATGCAGGTAGTTGTTTAGGAGATGGTACATTTTTTATATAACATTCACTTCCTTGTGTATTTGATAGTCTTTTCATGGTTTATAA
CATTTTCTCCTGTAAAGATAGGCTAATTTCTGAAATAATAATTAAATTTATAGAAAGCCGAGAGGAAATTGCTAGTTTATTCCTGGTAGA
GGAATTTCTGTATTTGAAAATTCTCCAGAAGGAATAATATAAACTGTGGACTTTGGGTGATAATGATATGTAGGTTCGTCAGTTGTTAAC
AAATGTATCCCTCTGTTGGGGGCTATTGATAATGGGGAAGGCTGTGCATGTGTGGGAGTAGGAGGTGTATGGGACATCTCTGTACCTTCT

>20673_20673_2_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000264414_SPAG16_chr2_214181943_ENST00000432529_length(amino acids)=615AA_BP=
MSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVR
EDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDR
GAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELIS
KHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESF
NNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDS
EKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYL

--------------------------------------------------------------
>20673_20673_3_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000344951_SPAG16_chr2_214174783_ENST00000331683_length(transcript)=3831nt_BP=2028nt
GCTCAGGCGGAGGAGGAGAAGGAGGAGGAGGAGGACGACGTTCGGCCTGCGCAGTGAGATGTTTGTCCGTCGCCGCCGCCGCCGCCATCG
CGGAGGAGCGCGATAAAGGGAGCCGAGCCGGACGTGAGGGGGACCCCGCGGAGCCGCCGCGCCAGCGCAGCCCCCCAGCCGCATCGGAGT
CGCCAGAGTCCGAGCCGCCGCCGCCGCCGCCGCCGCCCCCGCCGCCGCCGCGGCTGCCCAGGGGCCGGCCAGCTCCCCAGCCCTGCCCGG
GGGTCGCGCCGCCGCCGCCCCCAGCGTCGCCCCTAGCCTGCCTGCCCGCCTTAAATGTGACACCGGCGTCGCGGAGCGCGACCTGAAGCC
GCCGCCGGGGAGGGGACGAGCACCATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCG
GTGCGAGAAGATGTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATG
ATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGA
GATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTA
GACAGAGGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCT
TTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAA
GCTAGAATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTC
ATTTCCAAGCACATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGC
ATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTT
GTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAA
TCATTCAACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTC
TCATTATTTATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTT
TTTAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGAT
GACTCTGAAAAAAACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGC
ATCTCAAACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTC
ACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTC
TACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAA
AAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAG
ATGACCATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAACGGAAAACAGTTCTTCCTTCAAAGCATGCAGTACCTGAA
GTAATAGAAGACTTTCTCTGCAATTTCTTGATCAAAATGGGAATGACCAGAACTCTTGATTGCTTTCAGTCTGAATGGTATGAGTTAATA
CAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCCAGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTA
AAGAAAGATTTGAAGCACTACAAACAAGCAGCTGACAAAGCTAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATG
CATCATAAGCGAATAGTCCAGGAAAAAAACAAATTAATTAATGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATA
AGGGTGTTACATGAGAAACACCACACTTTACTGAAGGAGAAAATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGA
CTTCAAGAAACATTGAAGAAACTGCAAAGAGGACATAGTTACCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCA
CCAGAAGGTCCTACTCAGAAAGGTCTTCGTGAAGCCAGGGAACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGGATTCAGAA
TTTCCCATAGATATGCAACCAAATCCAAACCTGAATGTTTCTAAAGAAAGTCTTTCTCCAGCAAAATTTGACTACAAGCTGAAAAACATT
TTTAGACTCCATGAACTTCCAGTGAGCTGTGTCTCCATGCAACCCCACAAAGACATCCTAGTCTCCTGTGGCGAGGACCGACTCTGGAAG
GTGTTGGGCCTTCCAAAATGCAATGTGCTTCTCACGGGATTTGGCCACACTGACTGGCTTTCAGACTGCTGCTTCCATCCCAGTGGCGAC
AAATTGGCTACTTCAAGTGGTGACACTACAGTTAAATTATGGGATCTATGTAAAGGCGATTGCATTTTGACCTTTGAAGGACACAGCCGC
GCAGTGTGGTCCTGCACATGGCACTCCTGCGGCAATTTTGTGGCTTCCTCCTCACTGGATAAAACTAGCAAAATTTGGGATGTTAATAGT
GAAAGATGCAGATGTACTTTGTATGGACATACAGATTCTGTGAACAGCATTGAGTTTTTTCCTTTCTCCAATACTCTTCTCACAAGCTCT
GCAGACAAGACCCTGTCTATATGGGATGCAAGAACAGGTATATGTGAGCAGTCACTTTATGGTCACATGCATTCTATCAATGATGCCATT
TTTGATCCCAGGGGTCACATGATAGCATCCTGTGATGCCTGTGGGGTTACAAAGCTGTGGGACTTTCGGAAGCTGTTACCAATTGTGTCC
ATCGATATAGGTCCAAGTCCTGGCAATGAGGTGAATTTTGATTCATCAGGTCGAGTTTTAGCTCAGGCAAGTGGCAATGGTGTTATCCAT
TTGCTAGATCTTAAATCTGGGGAGATTCACAAATTGATGGGCCACGAAAACGAGGCACACACGGTTGTGTTTTCTCACGACGGGGAGATT
CTCTTTTCTGGAGGCTCTGACGGCACAGTTCGAACGTGGTCTTGACCGTCAGCACATCCCGCTGCAGAGGGCATTCCCTTTAAGGCTTGA
AAATGCCTCCTGTAGTCCCAAACCAGCAAAGAACTGGTTAAATGTGCCAGCAGGTAACATTTACAGTCTGCTTTAAAACATGCTTTGGAC

>20673_20673_3_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000344951_SPAG16_chr2_214174783_ENST00000331683_length(amino acids)=1086AA_BP=546
MSNLSKGTGSRKDTKMRIRAFPVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGC
IRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIE
RVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGK
NPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKD
VFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQ
SATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFN
NREKYTFEERKTVLPSKHAVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYK
QAADKAREDLLKIQKERDFHRMHHKRIVQEKNKLINDLKGLKLHYASYEPTIRVLHEKHHTLLKEKMLTSLERDKVVGQISGLQETLKKL
QRGHSYHGPQIKVDHSREKENAPEGPTQKGLREAREQNKCKTKMKGNTKDSEFPIDMQPNPNLNVSKESLSPAKFDYKLKNIFRLHELPV
SCVSMQPHKDILVSCGEDRLWKVLGLPKCNVLLTGFGHTDWLSDCCFHPSGDKLATSSGDTTVKLWDLCKGDCILTFEGHSRAVWSCTWH
SCGNFVASSSLDKTSKIWDVNSERCRCTLYGHTDSVNSIEFFPFSNTLLTSSADKTLSIWDARTGICEQSLYGHMHSINDAIFDPRGHMI
ASCDACGVTKLWDFRKLLPIVSIDIGPSPGNEVNFDSSGRVLAQASGNGVIHLLDLKSGEIHKLMGHENEAHTVVFSHDGEILFSGGSDG

--------------------------------------------------------------
>20673_20673_4_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000344951_SPAG16_chr2_214181943_ENST00000432529_length(transcript)=2788nt_BP=2028nt
GCTCAGGCGGAGGAGGAGAAGGAGGAGGAGGAGGACGACGTTCGGCCTGCGCAGTGAGATGTTTGTCCGTCGCCGCCGCCGCCGCCATCG
CGGAGGAGCGCGATAAAGGGAGCCGAGCCGGACGTGAGGGGGACCCCGCGGAGCCGCCGCGCCAGCGCAGCCCCCCAGCCGCATCGGAGT
CGCCAGAGTCCGAGCCGCCGCCGCCGCCGCCGCCGCCCCCGCCGCCGCCGCGGCTGCCCAGGGGCCGGCCAGCTCCCCAGCCCTGCCCGG
GGGTCGCGCCGCCGCCGCCCCCAGCGTCGCCCCTAGCCTGCCTGCCCGCCTTAAATGTGACACCGGCGTCGCGGAGCGCGACCTGAAGCC
GCCGCCGGGGAGGGGACGAGCACCATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCG
GTGCGAGAAGATGTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATG
ATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGA
GATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTA
GACAGAGGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCT
TTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAA
GCTAGAATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTC
ATTTCCAAGCACATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGC
ATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTT
GTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAA
TCATTCAACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTC
TCATTATTTATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTT
TTTAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGAT
GACTCTGAAAAAAACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGC
ATCTCAAACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTC
ACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTC
TACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAA
AAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAG
ATGACCATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGT
TGGGAATGTTCCAGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGC
AGCTGAGTATGTTATTTTTTAAATGACATTTTCTTCTTTTTCTTTTGGACTAAATAAAAGAGTTGAGTGAAGCTGATATATGTAATATAC
CAGAGCCTTAATTTTTGAAAACTGAATTTTTCTAGTTGTAAAGAATGTGAGAGGCTTCATTAGCAAATTAATTAAACAGATGATCAGAAC
TATCACAATTATAACTTACCAACAAGAAGGGAATGCAGGTAGTTGTTTAGGAGATGGTACATTTTTTATATAACATTCACTTCCTTGTGT
ATTTGATAGTCTTTTCATGGTTTATAACATTTTCTCCTGTAAAGATAGGCTAATTTCTGAAATAATAATTAAATTTATAGAAAGCCGAGA
GGAAATTGCTAGTTTATTCCTGGTAGAGGAATTTCTGTATTTGAAAATTCTCCAGAAGGAATAATATAAACTGTGGACTTTGGGTGATAA
TGATATGTAGGTTCGTCAGTTGTTAACAAATGTATCCCTCTGTTGGGGGCTATTGATAATGGGGAAGGCTGTGCATGTGTGGGAGTAGGA

>20673_20673_4_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000344951_SPAG16_chr2_214181943_ENST00000432529_length(amino acids)=549AA_BP=
MSNLSKGTGSRKDTKMRIRAFPVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGC
IRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIE
RVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGK
NPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKD
VFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQ
SATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFN

--------------------------------------------------------------
>20673_20673_5_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409096_SPAG16_chr2_214174783_ENST00000331683_length(transcript)=3716nt_BP=1913nt
GTAGAAAATTATTCACAGAGGACTAAAGACTTCTTGTCCTTTGTATGTAGTAGATGTTAAGAATTCACTTCTTTTCATTTAGTTTTAATT
TGTCTCATGATGTAGTTCTCTCAGTAATCACAGAACCTTCAAGATGTATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTC
TGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGC
ATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCAT
TGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGG
ACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTA
TTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTT
GCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTT
TTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAAC
GAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAG
TAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGC
CAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGA
ATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTA
AACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGA
AAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATG
TATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATCTA
AGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAAT
TCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGT
CAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGAC
AGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTG
TTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATA
ATAGAGAAAAATACACATTTGAGGAACGGAAAACAGTTCTTCCTTCAAAGCATGCAGTACCTGAAGTAATAGAAGACTTTCTCTGCAATT
TCTTGATCAAAATGGGAATGACCAGAACTCTTGATTGCTTTCAGTCTGAATGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAA
CTGTTGGGAATGTTCCAGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAAC
AAGCAGCTGACAAAGCTAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAA
AAAACAAATTAATTAATGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACA
CTTTACTGAAGGAGAAAATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGC
AAAGAGGACATAGTTACCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCACCAGAAGGTCCTACTCAGAAAGGTC
TTCGTGAAGCCAGGGAACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGGATTCAGAATTTCCCATAGATATGCAACCAAATC
CAAACCTGAATGTTTCTAAAGAAAGTCTTTCTCCAGCAAAATTTGACTACAAGCTGAAAAACATTTTTAGACTCCATGAACTTCCAGTGA
GCTGTGTCTCCATGCAACCCCACAAAGACATCCTAGTCTCCTGTGGCGAGGACCGACTCTGGAAGGTGTTGGGCCTTCCAAAATGCAATG
TGCTTCTCACGGGATTTGGCCACACTGACTGGCTTTCAGACTGCTGCTTCCATCCCAGTGGCGACAAATTGGCTACTTCAAGTGGTGACA
CTACAGTTAAATTATGGGATCTATGTAAAGGCGATTGCATTTTGACCTTTGAAGGACACAGCCGCGCAGTGTGGTCCTGCACATGGCACT
CCTGCGGCAATTTTGTGGCTTCCTCCTCACTGGATAAAACTAGCAAAATTTGGGATGTTAATAGTGAAAGATGCAGATGTACTTTGTATG
GACATACAGATTCTGTGAACAGCATTGAGTTTTTTCCTTTCTCCAATACTCTTCTCACAAGCTCTGCAGACAAGACCCTGTCTATATGGG
ATGCAAGAACAGGTATATGTGAGCAGTCACTTTATGGTCACATGCATTCTATCAATGATGCCATTTTTGATCCCAGGGGTCACATGATAG
CATCCTGTGATGCCTGTGGGGTTACAAAGCTGTGGGACTTTCGGAAGCTGTTACCAATTGTGTCCATCGATATAGGTCCAAGTCCTGGCA
ATGAGGTGAATTTTGATTCATCAGGTCGAGTTTTAGCTCAGGCAAGTGGCAATGGTGTTATCCATTTGCTAGATCTTAAATCTGGGGAGA
TTCACAAATTGATGGGCCACGAAAACGAGGCACACACGGTTGTGTTTTCTCACGACGGGGAGATTCTCTTTTCTGGAGGCTCTGACGGCA
CAGTTCGAACGTGGTCTTGACCGTCAGCACATCCCGCTGCAGAGGGCATTCCCTTTAAGGCTTGAAAATGCCTCCTGTAGTCCCAAACCA
GCAAAGAACTGGTTAAATGTGCCAGCAGGTAACATTTACAGTCTGCTTTAAAACATGCTTTGGACATATATTTTAGTGCTCATACTGTTA

>20673_20673_5_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409096_SPAG16_chr2_214174783_ENST00000331683_length(amino acids)=1158AA_BP=618
MLRIHFFSFSFNLSHDVVLSVITEPSRCMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEH
LINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERK
GEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVV
ERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDR
FLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNK
SVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEI
FRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEERKTVLPSKH
AVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAADKAREDLLKIQKERD
FHRMHHKRIVQEKNKLINDLKGLKLHYASYEPTIRVLHEKHHTLLKEKMLTSLERDKVVGQISGLQETLKKLQRGHSYHGPQIKVDHSRE
KENAPEGPTQKGLREAREQNKCKTKMKGNTKDSEFPIDMQPNPNLNVSKESLSPAKFDYKLKNIFRLHELPVSCVSMQPHKDILVSCGED
RLWKVLGLPKCNVLLTGFGHTDWLSDCCFHPSGDKLATSSGDTTVKLWDLCKGDCILTFEGHSRAVWSCTWHSCGNFVASSSLDKTSKIW
DVNSERCRCTLYGHTDSVNSIEFFPFSNTLLTSSADKTLSIWDARTGICEQSLYGHMHSINDAIFDPRGHMIASCDACGVTKLWDFRKLL

--------------------------------------------------------------
>20673_20673_6_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409096_SPAG16_chr2_214181943_ENST00000432529_length(transcript)=2673nt_BP=1913nt
GTAGAAAATTATTCACAGAGGACTAAAGACTTCTTGTCCTTTGTATGTAGTAGATGTTAAGAATTCACTTCTTTTCATTTAGTTTTAATT
TGTCTCATGATGTAGTTCTCTCAGTAATCACAGAACCTTCAAGATGTATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTC
TGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGC
ATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCAT
TGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGG
ACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTA
TTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTT
GCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTT
TTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAAC
GAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAG
TAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGC
CAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGA
ATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTA
AACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGA
AAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATG
TATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATCTA
AGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAAT
TCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGT
CAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGAC
AGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTG
TTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATA
ATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCCAGATGTCTACACC
CAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGAGTATGTTATTTTTTAAATG
ACATTTTCTTCTTTTTCTTTTGGACTAAATAAAAGAGTTGAGTGAAGCTGATATATGTAATATACCAGAGCCTTAATTTTTGAAAACTGA
ATTTTTCTAGTTGTAAAGAATGTGAGAGGCTTCATTAGCAAATTAATTAAACAGATGATCAGAACTATCACAATTATAACTTACCAACAA
GAAGGGAATGCAGGTAGTTGTTTAGGAGATGGTACATTTTTTATATAACATTCACTTCCTTGTGTATTTGATAGTCTTTTCATGGTTTAT
AACATTTTCTCCTGTAAAGATAGGCTAATTTCTGAAATAATAATTAAATTTATAGAAAGCCGAGAGGAAATTGCTAGTTTATTCCTGGTA
GAGGAATTTCTGTATTTGAAAATTCTCCAGAAGGAATAATATAAACTGTGGACTTTGGGTGATAATGATATGTAGGTTCGTCAGTTGTTA
ACAAATGTATCCCTCTGTTGGGGGCTATTGATAATGGGGAAGGCTGTGCATGTGTGGGAGTAGGAGGTGTATGGGACATCTCTGTACCTT

>20673_20673_6_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409096_SPAG16_chr2_214181943_ENST00000432529_length(amino acids)=621AA_BP=
MLRIHFFSFSFNLSHDVVLSVITEPSRCMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEH
LINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERK
GEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVV
ERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDR
FLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNK
SVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEI

--------------------------------------------------------------
>20673_20673_7_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000272898_length(transcript)=3022nt_BP=2376nt
AGGACAGAGTTCTCAGGGTGAAAATGATTTAAATGGCTCTGGAAAAGTAGTATACAAGAGATGTAGGGGAATAATGTTTTCTAAAAGAAA
GCTACGTTATACTGAGGTGCATTTATCATAGTGTTATTCTTAAGGTGCCTTTGTTTTAGCTCATATCTGTATATTTGCTAAGCTTGAAAA
AGCTATATTTCTTTCTGACATTTATTTGATGACTGTCGATGAGCGTTCAAGAGATTCTATCATTGGGCACTTTCCTTTGTTGATGCATGA
GGGATTATACTGTGCTCTGGGCTGTGTTAAATCTACTGACGTTCTCATTTAATCTTCATGACAGCCCTTTGAGATAGATGCTGTTATTGA
ACCCAGGACTTAATTTTCTCAAGGTCACATGGCTTGTGAGTAACAAAGCTGTGGCACAAACCTAAGGCTGTCTGACAGCAAAACCCATGT
TCTCTACCAGTATACTAACTATGTTTACCAAGATAATCAGTGACTGTGATGTTACTTTACCATATTAGCACAATGGAATGTGACATTAGT
CCACTGATTTGGACTAGGTTTTGGGATATATAAGTTCTAATCCCAGCTCTATGATTTTGGATGACCATGGATGAAAAATATGTAAACAGC
ATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATAT
ACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATA
CTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTA
CGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCA
ATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATG
TCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAAT
GAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCAC
ATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTA
TTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAA
GGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT
GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATT
GATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATG
CAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAA
AACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACA
ACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATAT
TGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAA
CACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGA
TCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTA
ATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCC
AGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGACAAAGC
TAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAAAAAACAAATTAATTAA
TGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACACTTTACTGAAGGAGAA
AATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGCAAAGAGGACATAGTTA
CCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCACCAGAAGGTCCTACTCAGAAAGGTCTTCGTGAAGCCAGGGA
ACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGCAGTGGACAGGGAACTCTCAAACTTGGAATAAGACCGTACCTCTGCAGGA

>20673_20673_7_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000272898_length(amino acids)=593AA_BP=
MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDH
QTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE
EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGK
TEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS
RSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEG
MFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNAT

--------------------------------------------------------------
>20673_20673_8_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000331683_length(transcript)=4060nt_BP=2376nt
AGGACAGAGTTCTCAGGGTGAAAATGATTTAAATGGCTCTGGAAAAGTAGTATACAAGAGATGTAGGGGAATAATGTTTTCTAAAAGAAA
GCTACGTTATACTGAGGTGCATTTATCATAGTGTTATTCTTAAGGTGCCTTTGTTTTAGCTCATATCTGTATATTTGCTAAGCTTGAAAA
AGCTATATTTCTTTCTGACATTTATTTGATGACTGTCGATGAGCGTTCAAGAGATTCTATCATTGGGCACTTTCCTTTGTTGATGCATGA
GGGATTATACTGTGCTCTGGGCTGTGTTAAATCTACTGACGTTCTCATTTAATCTTCATGACAGCCCTTTGAGATAGATGCTGTTATTGA
ACCCAGGACTTAATTTTCTCAAGGTCACATGGCTTGTGAGTAACAAAGCTGTGGCACAAACCTAAGGCTGTCTGACAGCAAAACCCATGT
TCTCTACCAGTATACTAACTATGTTTACCAAGATAATCAGTGACTGTGATGTTACTTTACCATATTAGCACAATGGAATGTGACATTAGT
CCACTGATTTGGACTAGGTTTTGGGATATATAAGTTCTAATCCCAGCTCTATGATTTTGGATGACCATGGATGAAAAATATGTAAACAGC
ATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATAT
ACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATA
CTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTA
CGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCA
ATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATG
TCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAAT
GAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCAC
ATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTA
TTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAA
GGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT
GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATT
GATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATG
CAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAA
AACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACA
ACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATAT
TGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAA
CACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGA
TCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTA
ATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCC
AGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGACAAAGC
TAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAAAAAACAAATTAATTAA
TGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACACTTTACTGAAGGAGAA
AATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGCAAAGAGGACATAGTTA
CCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCACCAGAAGGTCCTACTCAGAAAGGTCTTCGTGAAGCCAGGGA
ACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGGATTCAGAATTTCCCATAGATATGCAACCAAATCCAAACCTGAATGTTTC
TAAAGAAAGTCTTTCTCCAGCAAAATTTGACTACAAGCTGAAAAACATTTTTAGACTCCATGAACTTCCAGTGAGCTGTGTCTCCATGCA
ACCCCACAAAGACATCCTAGTCTCCTGTGGCGAGGACCGACTCTGGAAGGTGTTGGGCCTTCCAAAATGCAATGTGCTTCTCACGGGATT
TGGCCACACTGACTGGCTTTCAGACTGCTGCTTCCATCCCAGTGGCGACAAATTGGCTACTTCAAGTGGTGACACTACAGTTAAATTATG
GGATCTATGTAAAGGCGATTGCATTTTGACCTTTGAAGGACACAGCCGCGCAGTGTGGTCCTGCACATGGCACTCCTGCGGCAATTTTGT
GGCTTCCTCCTCACTGGATAAAACTAGCAAAATTTGGGATGTTAATAGTGAAAGATGCAGATGTACTTTGTATGGACATACAGATTCTGT
GAACAGCATTGAGTTTTTTCCTTTCTCCAATACTCTTCTCACAAGCTCTGCAGACAAGACCCTGTCTATATGGGATGCAAGAACAGGTAT
ATGTGAGCAGTCACTTTATGGTCACATGCATTCTATCAATGATGCCATTTTTGATCCCAGGGGTCACATGATAGCATCCTGTGATGCCTG
TGGGGTTACAAAGCTGTGGGACTTTCGGAAGCTGTTACCAATTGTGTCCATCGATATAGGTCCAAGTCCTGGCAATGAGGTGAATTTTGA
TTCATCAGGTCGAGTTTTAGCTCAGGCAAGTGGCAATGGTGTTATCCATTTGCTAGATCTTAAATCTGGGGAGATTCACAAATTGATGGG
CCACGAAAACGAGGCACACACGGTTGTGTTTTCTCACGACGGGGAGATTCTCTTTTCTGGAGGCTCTGACGGCACAGTTCGAACGTGGTC
TTGACCGTCAGCACATCCCGCTGCAGAGGGCATTCCCTTTAAGGCTTGAAAATGCCTCCTGTAGTCCCAAACCAGCAAAGAACTGGTTAA
ATGTGCCAGCAGGTAACATTTACAGTCTGCTTTAAAACATGCTTTGGACATATATTTTAGTGCTCATACTGTTACTAATAAAAAAATAAC

>20673_20673_8_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000331683_length(amino acids)=593AA_BP=
MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDH
QTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE
EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGK
TEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS
RSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEG
MFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNAT

--------------------------------------------------------------
>20673_20673_9_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000374309_length(transcript)=3874nt_BP=2376nt
AGGACAGAGTTCTCAGGGTGAAAATGATTTAAATGGCTCTGGAAAAGTAGTATACAAGAGATGTAGGGGAATAATGTTTTCTAAAAGAAA
GCTACGTTATACTGAGGTGCATTTATCATAGTGTTATTCTTAAGGTGCCTTTGTTTTAGCTCATATCTGTATATTTGCTAAGCTTGAAAA
AGCTATATTTCTTTCTGACATTTATTTGATGACTGTCGATGAGCGTTCAAGAGATTCTATCATTGGGCACTTTCCTTTGTTGATGCATGA
GGGATTATACTGTGCTCTGGGCTGTGTTAAATCTACTGACGTTCTCATTTAATCTTCATGACAGCCCTTTGAGATAGATGCTGTTATTGA
ACCCAGGACTTAATTTTCTCAAGGTCACATGGCTTGTGAGTAACAAAGCTGTGGCACAAACCTAAGGCTGTCTGACAGCAAAACCCATGT
TCTCTACCAGTATACTAACTATGTTTACCAAGATAATCAGTGACTGTGATGTTACTTTACCATATTAGCACAATGGAATGTGACATTAGT
CCACTGATTTGGACTAGGTTTTGGGATATATAAGTTCTAATCCCAGCTCTATGATTTTGGATGACCATGGATGAAAAATATGTAAACAGC
ATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATAT
ACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATA
CTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTA
CGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCA
ATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATG
TCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAAT
GAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCAC
ATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTA
TTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAA
GGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT
GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATT
GATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATG
CAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAA
AACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACA
ACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATAT
TGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAA
CACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGA
TCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTA
ATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCC
AGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGACAAAGC
TAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAAAAAACAAATTAATTAA
TGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACACTTTACTGAAGGAGAA
AATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGCAAAGAGGACATAGTTA
CCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCACCAGAAGGTCCTACTCAGAAAGGTCTTCGTGAAGCCAGGGA
ACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGGATTCAGAATTTCCCATAGATATGCAACCAAATCCAAACCTGAATGTTTC
TAAAGAAAGTCTTTCTCCAGCAAAATTTGACTACAAGCTGAAAAACATTTTTAGACTCCATGAACTTCCAGTGAGCTGTGTCTCCATGCA
ACCCCACAAAGACATCCTAGTCTCCTGTGGCGAGGACCGACTCTGGAAGGTGTTGGGCCTTCCAAAATGCAATGTGCTTCTCACGGGATT
TGGCCACACTGACTGGCTTTCAGACTGCTGCTTCCATCCCAGTGGCGACAAATTGGCTACTTCAAGTGGTGACACTACAGTTAAATTATG
GGATCTATGTAAAGGCGATTGCATTTTGACCTTTGAAGGACACAGCCGCGCAGTGTGGTCCTGCACATGGCACTCCTGCGGCAATTTTGT
GGCTTCCTCCTCACTGGATAAAACTAGCAAAATTTGGGATGTTAATAGTGAAAGATGCAGATGTACTTTGTATGGACATACAGATTCTGT
GAACAGCATTGAGTTTTTTCCTTTCTCCAATACTCTTCTCACAAGCTCTGCAGACAAGACCCTGTCTATATGGGATGCAAGAACAGGTAT
ATGTGAGCAGTCACTTTATGGTCACATGCATTCTATCAATGATGCCATTTTTGATCCCAGGGGTCACATGATAGCATCCTGTGATGCCTG
TGGGGTTACAAAGCTGTGGGACTTTCGGAAGCTGTTACCAATTGTGTCCATCGATATAGGTCCAAGTCCTGGCAATGAGGTGAATTTTGA
TTCATCAGGTCGAGTTTTAGCTCAGGCAAGTGGCAATGGTGTTATCCATTTGCTAGATCTTAAATCTGGGGAGATTCACAAATTGATGGG
CCACGAAAACGAGGCACACACGGTTGTGTTTTCTCACGACGGGGAGATTCTCTTTTCTGGAGGCTCTGACGGCACAGTTCGAACGTGGTC

>20673_20673_9_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000374309_length(amino acids)=593AA_BP=
MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDH
QTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE
EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGK
TEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS
RSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEG
MFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNAT

--------------------------------------------------------------
>20673_20673_10_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000413312_length(transcript)=4081nt_BP=2376nt
AGGACAGAGTTCTCAGGGTGAAAATGATTTAAATGGCTCTGGAAAAGTAGTATACAAGAGATGTAGGGGAATAATGTTTTCTAAAAGAAA
GCTACGTTATACTGAGGTGCATTTATCATAGTGTTATTCTTAAGGTGCCTTTGTTTTAGCTCATATCTGTATATTTGCTAAGCTTGAAAA
AGCTATATTTCTTTCTGACATTTATTTGATGACTGTCGATGAGCGTTCAAGAGATTCTATCATTGGGCACTTTCCTTTGTTGATGCATGA
GGGATTATACTGTGCTCTGGGCTGTGTTAAATCTACTGACGTTCTCATTTAATCTTCATGACAGCCCTTTGAGATAGATGCTGTTATTGA
ACCCAGGACTTAATTTTCTCAAGGTCACATGGCTTGTGAGTAACAAAGCTGTGGCACAAACCTAAGGCTGTCTGACAGCAAAACCCATGT
TCTCTACCAGTATACTAACTATGTTTACCAAGATAATCAGTGACTGTGATGTTACTTTACCATATTAGCACAATGGAATGTGACATTAGT
CCACTGATTTGGACTAGGTTTTGGGATATATAAGTTCTAATCCCAGCTCTATGATTTTGGATGACCATGGATGAAAAATATGTAAACAGC
ATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATAT
ACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATA
CTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTA
CGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCA
ATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATG
TCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAAT
GAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCAC
ATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTA
TTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAA
GGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT
GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATT
GATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATG
CAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAA
AACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACA
ACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATAT
TGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAA
CACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGA
TCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTA
ATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCC
AGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGACAAAGC
TAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAAAAAACAAATTAATTAA
TGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACACTTTACTGAAGGAGAA
AATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGCAAAGAGGACATAGTTA
CCATGGTCCTCAAATTAAAGGTAAATGTAAAATGTGATGAAGCTCTCATTTAATATAGTTTGGTGATTTCTCAACCTTATCAGTGATTAA
TATTCTAGAGATATTTAAAGGTGAGTAAGTGGTGGCTATTTCACAAATTAAAATTTTAATAAAAAGATTAAGAAGAAATATAGATATCAT
ACTGTATGGATTAATTATAGAGAAAAACAAGTCAACTATAATGGAAGGACTTCTGCTTCTGGTCATTATACCTGGTACCAGACTAGCCTT
CTCTGTGTAAATAACTCTAAAGCTAAGCTGTAAGATCAGACAACTACTTTTAGGCAGTAGAAAACAGACAGCTCAAGTAACAGAAGGCAA
TTGACAGAAGGAAAATTTATAAGGTGAGCTCTGCCATCACCATGGCTTTGGGTCTGTGGAAAGTTCCAAACTGAGCTTTGTGGAGATGGA
AGCCAATAAGAGCATGACAATCCTGATAATTGGAAATGTCAGAGACCCTAGTTCACAGCTTTTGAAGCCACTGGAATCTGTAGGGAAAGA
CACCAGAGAAGAGAGCTGCAATGAGGAGTGGCTCAGTTTGTGAGGGAGTTTACTATGAGTCCTTAGCTGAGGCTGCAATACTATACATAA
CAACACTACCTGACACTTACAAGAGAGATACAATTATATAGGATCAAGAGGAGGCCAGAGATTGTAGAAGTTAAGTAGTGATGCGAAACA
TTGCAAATCTGGCCATGCCTGGGTGGAAAGACCTCTTTCACACCTTGTTAACAACACAGTAACATTCAGCATCCCATATCCGCCTTATAA
AACCAGGAACAAAGCTTACACAGCATTCTTAGAGAGGACACAGAATTTGGAGTTTGAGTCTTGCCAAGTTATAGGGCCTTGAGAAACATT
TAGGGCTTTCCATGGATCCACCCTAACGAAGCATAAAATTAAGCCTAGGATTTTAGGGTCATCAGCCAAAAATGGAACTGCCTTCTAGAA
CAAAAAATGACATCCTTTTGAGGAAGACAGTCATCCAGAGTCTTTACAATCTTTTACCCACATTGCCTAGTACATAATTAAACATTTCTA
GATATGAATAGGAACAGGAAAATGTGACCCATAATCAAGACAACAAGCAATAAATGGAAACCTACCCTTAAGTAGCTAAACTGTTGCATT
TAGCAGATAGAAAGTGCTAAAGTAGATACTTTGATTATGTTCAAGGACTTAATGGAAAATACTAATAAGTGAACAGATGGGGAATTTAAG

>20673_20673_10_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000413312_length(amino acids)=593AA_BP=
MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDH
QTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE
EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGK
TEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS
RSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEG
MFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNAT

--------------------------------------------------------------
>20673_20673_11_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000447990_length(transcript)=3305nt_BP=2376nt
AGGACAGAGTTCTCAGGGTGAAAATGATTTAAATGGCTCTGGAAAAGTAGTATACAAGAGATGTAGGGGAATAATGTTTTCTAAAAGAAA
GCTACGTTATACTGAGGTGCATTTATCATAGTGTTATTCTTAAGGTGCCTTTGTTTTAGCTCATATCTGTATATTTGCTAAGCTTGAAAA
AGCTATATTTCTTTCTGACATTTATTTGATGACTGTCGATGAGCGTTCAAGAGATTCTATCATTGGGCACTTTCCTTTGTTGATGCATGA
GGGATTATACTGTGCTCTGGGCTGTGTTAAATCTACTGACGTTCTCATTTAATCTTCATGACAGCCCTTTGAGATAGATGCTGTTATTGA
ACCCAGGACTTAATTTTCTCAAGGTCACATGGCTTGTGAGTAACAAAGCTGTGGCACAAACCTAAGGCTGTCTGACAGCAAAACCCATGT
TCTCTACCAGTATACTAACTATGTTTACCAAGATAATCAGTGACTGTGATGTTACTTTACCATATTAGCACAATGGAATGTGACATTAGT
CCACTGATTTGGACTAGGTTTTGGGATATATAAGTTCTAATCCCAGCTCTATGATTTTGGATGACCATGGATGAAAAATATGTAAACAGC
ATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATAT
ACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATA
CTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTA
CGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCA
ATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATG
TCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAAT
GAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCAC
ATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTA
TTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAA
GGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT
GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATT
GATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATG
CAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAA
AACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACA
ACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATAT
TGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAA
CACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGA
TCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTA
ATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGTATGAGTTAATACAGAAAGGAGTGACTGAACTTAGAACTGTTGGGAATGTTCC
AGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGAACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGACAAAGC
TAGAGAAGATTTGCTGAAAATTCAGAAAGAACGTGATTTTCATCGAATGCATCATAAGCGAATAGTCCAGGAAAAAAACAAATTAATTAA
TGACCTCAAAGGGTTGAAGTTACATTATGCATCTTATGAACCGACTATAAGGGTGTTACATGAGAAACACCACACTTTACTGAAGGAGAA
AATGCTGACCTCCTTGGAAAGAGACAAAGTAGTTGGGCAGATTTCTGGACTTCAAGAAACATTGAAGAAACTGCAAAGAGGACATAGTTA
CCATGGTCCTCAAATTAAAGTTGATCATAGTCGTGAAAAAGAAAATGCACCAGAAGGTCCTACTCAGAAAGGTCTTCGTGAAGCCAGGGA
ACAAAACAAATGTAAAACAAAGATGAAAGGCAATACAAAGGTGATGAGAAGGAGAGAAGAGCTGTGGCCCTTCAGGGAGCCCAGACCTAG
AAGCTCCACGAGCCAGTGCTGTGACACCCTCTTTGGGGCTGTGCAGTTTCAGATGTTTCCAAGCATCCAGACGCCACTGAATTCTCATCC
AGATTTGGGTGCCCACAGCAGAAGTTGCTTGGAGTGCATCTGATCCAGCTCCAGGCTTACACAGATCCAGTGCCTGTGCCAGTGCCTGGA
GCTGCCTGCCCCACTGCAGCAGCTGGCATGCCTGGCTGTGCACAGTGGCTGGACCTGGTTCTCACTTGCTCACACACCCCTCACCTCTCT

>20673_20673_11_CUL3-SPAG16_CUL3_chr2_225360549_ENST00000409777_SPAG16_chr2_214181943_ENST00000447990_length(amino acids)=593AA_BP=
MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDH
QTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE
EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGK
TEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS
RSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEG
MFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNAT

--------------------------------------------------------------

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Fusion Gene PPI Analysis for CUL3-SPAG16


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with
HgeneCUL3chr2:225360549chr2:214174783ENST00000264414-13162_41614.0769.0KLHL18
HgeneCUL3chr2:225360549chr2:214174783ENST00000344951-12152_41548.0703.0KLHL18
HgeneCUL3chr2:225360549chr2:214174783ENST00000409096-13162_41590.0745.0KLHL18
HgeneCUL3chr2:225360549chr2:214174783ENST00000409777-13162_41590.0745.0KLHL18
HgeneCUL3chr2:225360549chr2:214181943ENST00000264414-13162_41614.0769.0KLHL18
HgeneCUL3chr2:225360549chr2:214181943ENST00000344951-12152_41548.0703.0KLHL18
HgeneCUL3chr2:225360549chr2:214181943ENST00000409096-13162_41590.0745.0KLHL18
HgeneCUL3chr2:225360549chr2:214181943ENST00000409777-13162_41590.0745.0KLHL18


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CUL3-SPAG16


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CUL3-SPAG16


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource