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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:ADAMTS5-DLC1 (FusionGDB2 ID:2097) |
Fusion Gene Summary for ADAMTS5-DLC1 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: ADAMTS5-DLC1 | Fusion gene ID: 2097 | Hgene | Tgene | Gene symbol | ADAMTS5 | DLC1 | Gene ID | 11096 | 10395 |
| Gene name | ADAM metallopeptidase with thrombospondin type 1 motif 5 | DLC1 Rho GTPase activating protein | |
| Synonyms | ADAM-TS 11|ADAM-TS 5|ADAM-TS5|ADAMTS-11|ADAMTS-5|ADAMTS11|ADMP-2 | ARHGAP7|HP|STARD12|p122-RhoGAP | |
| Cytomap | 21q21.3 | 8p22 | |
| Type of gene | protein-coding | protein-coding | |
| Description | A disintegrin and metalloproteinase with thrombospondin motifs 5a disintegrin and metalloproteinase with thrombospondin motifs 11a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)aggrecanase-2 | rho GTPase-activating protein 7Rho-GTPase-activating protein 7START domain-containing protein 12StAR-related lipid transfer (START) domain containing 12deleted in liver cancer 1 proteindeleted in liver cancer 1 variant 2deleted in liver cancer varia | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q9UNA0 | Q96QB1 | |
| Ensembl transtripts involved in fusion gene | ENST00000284987, | ENST00000520226, ENST00000316609, ENST00000510318, ENST00000511869, ENST00000276297, ENST00000358919, ENST00000512044, | |
| Fusion gene scores | * DoF score | 3 X 4 X 3=36 | 17 X 14 X 5=1190 |
| # samples | 4 | 19 | |
| ** MAII score | log2(4/36*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(19/1190*10)=-2.64689024986436 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: ADAMTS5 [Title/Abstract] AND DLC1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | ADAMTS5(28337606)-DLC1(12973166), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | ADAMTS5-DLC1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. ADAMTS5-DLC1 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | DLC1 | GO:0006915 | apoptotic process | 17292327 |
| Tgene | DLC1 | GO:0006919 | activation of cysteine-type endopeptidase activity involved in apoptotic process | 17888903 |
| Tgene | DLC1 | GO:0008285 | negative regulation of cell proliferation | 12545165|17932950 |
| Tgene | DLC1 | GO:0030336 | negative regulation of cell migration | 17932950|19158340 |
| Tgene | DLC1 | GO:0035307 | positive regulation of protein dephosphorylation | 17292327 |
| Tgene | DLC1 | GO:0051497 | negative regulation of stress fiber assembly | 17932950 |
| Tgene | DLC1 | GO:0051895 | negative regulation of focal adhesion assembly | 19158340 |
| Tgene | DLC1 | GO:1900119 | positive regulation of execution phase of apoptosis | 17888903 |
| Fusion gene breakpoints across ADAMTS5 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across DLC1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | STAD | TCGA-BR-A4J8 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
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Fusion Gene ORF analysis for ADAMTS5-DLC1 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-5UTR | ENST00000284987 | ENST00000520226 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| 5CDS-intron | ENST00000284987 | ENST00000316609 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| 5CDS-intron | ENST00000284987 | ENST00000510318 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| 5CDS-intron | ENST00000284987 | ENST00000511869 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| Frame-shift | ENST00000284987 | ENST00000276297 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| Frame-shift | ENST00000284987 | ENST00000358919 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| Frame-shift | ENST00000284987 | ENST00000512044 | ADAMTS5 | chr21 | 28337606 | - | DLC1 | chr8 | 12973166 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for ADAMTS5-DLC1 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for ADAMTS5-DLC1 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:28337606/:12973166) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| ADAMTS5 | DLC1 |
| FUNCTION: Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection. {ECO:0000250|UniProtKB:Q9R001, ECO:0000269|PubMed:16133547, ECO:0000269|PubMed:18992360}. | FUNCTION: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. {ECO:0000269|PubMed:18786931, ECO:0000269|PubMed:19170769, ECO:0000269|PubMed:19710422}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for ADAMTS5-DLC1 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for ADAMTS5-DLC1 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for ADAMTS5-DLC1 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for ADAMTS5-DLC1 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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