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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:DAPK3-NFATC1 (FusionGDB2 ID:21368) |
Fusion Gene Summary for DAPK3-NFATC1 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: DAPK3-NFATC1 | Fusion gene ID: 21368 | Hgene | Tgene | Gene symbol | DAPK3 | NFATC1 | Gene ID | 1613 | 4772 |
| Gene name | death associated protein kinase 3 | nuclear factor of activated T cells 1 | |
| Synonyms | DLK|ZIP|ZIPK | NF-ATC|NF-ATc1.2|NFAT2|NFATc | |
| Cytomap | 19p13.3 | 18q23 | |
| Type of gene | protein-coding | protein-coding | |
| Description | death-associated protein kinase 3DAP kinase 3DAP-like kinaseMYPT1 kinaseZIP-kinasezipper-interacting protein kinase | nuclear factor of activated T-cells, cytoplasmic 1NFAT transcription complex cytosolic componentnuclear factor of activated T-cells 'c'nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 | |
| Modification date | 20200329 | 20200313 | |
| UniProtAcc | O43293 | O95644 | |
| Ensembl transtripts involved in fusion gene | ENST00000301264, ENST00000545797, | ENST00000253506, ENST00000318065, ENST00000329101, ENST00000397790, ENST00000427363, ENST00000542384, ENST00000545796, ENST00000586434, ENST00000587635, ENST00000590172, ENST00000591814, ENST00000592223, | |
| Fusion gene scores | * DoF score | 1 X 1 X 1=1 | 9 X 9 X 7=567 |
| # samples | 1 | 11 | |
| ** MAII score | log2(1/1*10)=3.32192809488736 | log2(11/567*10)=-2.36584521141757 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: DAPK3 [Title/Abstract] AND NFATC1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | DAPK3(3964370)-NFATC1(77272307), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | DAPK3 | GO:0006468 | protein phosphorylation | 10356987 |
| Hgene | DAPK3 | GO:0006915 | apoptotic process | 10580117 |
| Hgene | DAPK3 | GO:0017148 | negative regulation of translation | 18995835 |
| Hgene | DAPK3 | GO:0035556 | intracellular signal transduction | 10356987 |
| Hgene | DAPK3 | GO:0043065 | positive regulation of apoptotic process | 21487036 |
| Hgene | DAPK3 | GO:0046777 | protein autophosphorylation | 18239682 |
| Hgene | DAPK3 | GO:0051893 | regulation of focal adhesion assembly | 23454120 |
| Hgene | DAPK3 | GO:0071346 | cellular response to interferon-gamma | 18995835 |
| Hgene | DAPK3 | GO:2000249 | regulation of actin cytoskeleton reorganization | 23454120 |
| Tgene | NFATC1 | GO:0033173 | calcineurin-NFAT signaling cascade | 14979875 |
| Tgene | NFATC1 | GO:0035556 | intracellular signal transduction | 14749367 |
| Tgene | NFATC1 | GO:0045893 | positive regulation of transcription, DNA-templated | 14749367|14979875 |
| Tgene | NFATC1 | GO:0045944 | positive regulation of transcription by RNA polymerase II | 14979875 |
| Tgene | NFATC1 | GO:1905064 | negative regulation of vascular smooth muscle cell differentiation | 23853098 |
| Fusion gene breakpoints across DAPK3 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across NFATC1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChiTaRS5.0 | N/A | BI045301 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
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Fusion Gene ORF analysis for DAPK3-NFATC1 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| intron-intron | ENST00000301264 | ENST00000253506 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000318065 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000329101 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000397790 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000427363 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000542384 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000545796 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000586434 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000587635 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000590172 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000591814 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000301264 | ENST00000592223 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000253506 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000318065 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000329101 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000397790 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000427363 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000542384 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000545796 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000586434 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000587635 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000590172 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000591814 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| intron-intron | ENST00000545797 | ENST00000592223 | DAPK3 | chr19 | 3964370 | + | NFATC1 | chr18 | 77272307 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for DAPK3-NFATC1 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for DAPK3-NFATC1 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:3964370/:77272307) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| DAPK3 | NFATC1 |
| FUNCTION: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor. {ECO:0000269|PubMed:10356987, ECO:0000269|PubMed:11384979, ECO:0000269|PubMed:11781833, ECO:0000269|PubMed:12917339, ECO:0000269|PubMed:15096528, ECO:0000269|PubMed:15367680, ECO:0000269|PubMed:16219639, ECO:0000269|PubMed:17126281, ECO:0000269|PubMed:17158456, ECO:0000269|PubMed:18084323, ECO:0000269|PubMed:18995835, ECO:0000269|PubMed:21169990, ECO:0000269|PubMed:21408167, ECO:0000269|PubMed:21454679, ECO:0000269|PubMed:21487036, ECO:0000269|PubMed:23454120}. | FUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed:10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity). {ECO:0000250|UniProtKB:O88942, ECO:0000269|PubMed:10358178}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for DAPK3-NFATC1 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for DAPK3-NFATC1 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for DAPK3-NFATC1 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for DAPK3-NFATC1 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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