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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:DBT-WDR45 (FusionGDB2 ID:21467)

Fusion Gene Summary for DBT-WDR45

check button Fusion gene summary
Fusion gene informationFusion gene name: DBT-WDR45
Fusion gene ID: 21467
HgeneTgene
Gene symbol

DBT

WDR45

Gene ID

1629

11152

Gene namedihydrolipoamide branched chain transacylase E2WD repeat domain 45
SynonymsBCATE2|BCKAD-E2|BCKADE2|BCOADC-E2|E2|E2BJM5|NBIA4|NBIA5|WDRX1|WIPI-4|WIPI4
Cytomap

1p21.2

Xp11.23

Type of geneprotein-codingprotein-coding
Descriptionlipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial52 kDa mitochondrial autoantigen of primary biliary cirrhosisBCKAD E2 subunitE2 component of branched chain alpha-keto acid dehydrogenase complexWD repeat domain phosphoinositide-interacting protein 4WD repeat domain, X-linked 1WD repeat-containing protein 45WD45 repeat protein interacting with phosphoinositides 4neurodegeneration with brain iron accumulation 4neurodegeneration with brain iro
Modification date2020031320200313
UniProtAcc

P11182

.
Ensembl transtripts involved in fusion geneENST00000370131, ENST00000370132, 
ENST00000376372, ENST00000322995, 
ENST00000356463, ENST00000376368, 
ENST00000396681, ENST00000465431, 
ENST00000473974, ENST00000485908, 
ENST00000553851, 
Fusion gene scores* DoF score5 X 4 X 4=803 X 3 X 2=18
# samples 54
** MAII scorelog2(5/80*10)=-0.678071905112638
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(4/18*10)=1.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: DBT [Title/Abstract] AND WDR45 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointDBT(100690572)-WDR45(48932299), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneWDR45

GO:0009267

cellular response to starvation

28561066


check buttonFusion gene breakpoints across DBT (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across WDR45 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/AAA079679DBTchr1

100690572

-WDR45chrX

48932299

+


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Fusion Gene ORF analysis for DBT-WDR45

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-3UTRENST00000370131ENST00000376372DBTchr1

100690572

-WDR45chrX

48932299

+
intron-3UTRENST00000370132ENST00000376372DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000322995DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000356463DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000376368DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000396681DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000465431DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000473974DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000485908DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370131ENST00000553851DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000322995DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000356463DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000376368DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000396681DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000465431DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000473974DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000485908DBTchr1

100690572

-WDR45chrX

48932299

+
intron-intronENST00000370132ENST00000553851DBTchr1

100690572

-WDR45chrX

48932299

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for DBT-WDR45


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for DBT-WDR45


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:100690572/:48932299)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DBT

P11182

.
FUNCTION: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for DBT-WDR45


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for DBT-WDR45


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for DBT-WDR45


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for DBT-WDR45


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource