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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ADD1-GRK4 (FusionGDB2 ID:2290)

Fusion Gene Summary for ADD1-GRK4

check button Fusion gene summary
Fusion gene informationFusion gene name: ADD1-GRK4
Fusion gene ID: 2290
HgeneTgene
Gene symbol

ADD1

GRK4

Gene ID

118

2868

Gene nameadducin 1G protein-coupled receptor kinase 4
SynonymsADDAGPRK2L|GPRK4|GRK4a|IT11
Cytomap

4p16.3

4p16.3

Type of geneprotein-codingprotein-coding
Descriptionalpha-adducinadducin 1 (alpha)erythrocyte adducin alpha subunitG protein-coupled receptor kinase 4G protein-coupled receptor kinase 2-like
Modification date2020032220200313
UniProtAcc

P35611

P32298

Ensembl transtripts involved in fusion geneENST00000264758, ENST00000355842, 
ENST00000398123, ENST00000398125, 
ENST00000398129, ENST00000446856, 
ENST00000503455, ENST00000513328, 
ENST00000538860, 		ENST00000442472, 
ENST00000503518, ENST00000509545, 
ENST00000345167, ENST00000398051, 
ENST00000398052, ENST00000504933, 
Fusion gene scores* DoF score15 X 13 X 8=15607 X 7 X 4=196
# samples 197
** MAII scorelog2(19/1560*10)=-3.03747470541866
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(7/196*10)=-1.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ADD1 [Title/Abstract] AND GRK4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointADD1(2916767)-GRK4(3021367), # samples:1
GRK4(2988522)-ADD1(2922961), # samples:1
Anticipated loss of major functional domain due to fusion event.ADD1-GRK4 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
ADD1-GRK4 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
ADD1-GRK4 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneADD1

GO:0032092

positive regulation of protein binding

3693401|7642559

HgeneADD1

GO:0051016

barbed-end actin filament capping

8626479

HgeneADD1

GO:0051017

actin filament bundle assembly

3693401

HgeneADD1

GO:0071277

cellular response to calcium ion

25978380

HgeneADD1

GO:1903142

positive regulation of establishment of endothelial barrier

25978380

HgeneADD1

GO:1903393

positive regulation of adherens junction organization

25978380

TgeneGRK4

GO:0031623

receptor internalization

20074556


check buttonFusion gene breakpoints across ADD1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across GRK4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4ESCATCGA-LN-A49LADD1chr4

2916767

+GRK4chr4

3021367

+


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Fusion Gene ORF analysis for ADD1-GRK4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000264758ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000264758ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000264758ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000355842ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000355842ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000355842ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398123ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398123ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398123ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398125ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398125ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398125ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398129ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398129ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000398129ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000446856ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000446856ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000446856ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000503455ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000503455ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000503455ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000513328ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000513328ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
5CDS-intronENST00000513328ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000264758ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000264758ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000264758ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000264758ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000355842ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000355842ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000355842ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000355842ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398123ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398123ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398123ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398123ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398125ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398125ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398125ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398125ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398129ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398129ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398129ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000398129ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000446856ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000446856ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000446856ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000446856ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000503455ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000503455ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000503455ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000503455ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000513328ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000513328ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000513328ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
Frame-shiftENST00000513328ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-3CDSENST00000538860ENST00000345167ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-3CDSENST00000538860ENST00000398051ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-3CDSENST00000538860ENST00000398052ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-3CDSENST00000538860ENST00000504933ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-intronENST00000538860ENST00000442472ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-intronENST00000538860ENST00000503518ADD1chr4

2916767

+GRK4chr4

3021367

+
intron-intronENST00000538860ENST00000509545ADD1chr4

2916767

+GRK4chr4

3021367

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ADD1-GRK4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ADD1chr42916767+GRK4chr43021367+1.07E-050.9999893
ADD1chr42916767+GRK4chr43021367+1.07E-050.9999893

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for ADD1-GRK4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:2916767/:3021367)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ADD1

P35611

GRK4

P32298

FUNCTION: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.FUNCTION: Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. {ECO:0000269|PubMed:19520868, ECO:0000269|PubMed:8626439}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ADD1-GRK4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ADD1-GRK4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ADD1-GRK4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ADD1-GRK4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource