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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:DLGAP4-HCK (FusionGDB2 ID:23167)

Fusion Gene Summary for DLGAP4-HCK

check button Fusion gene summary
Fusion gene informationFusion gene name: DLGAP4-HCK
Fusion gene ID: 23167
HgeneTgene
Gene symbol

DLGAP4

HCK

Gene ID

22839

3055

Gene nameDLG associated protein 4HCK proto-oncogene, Src family tyrosine kinase
SynonymsDAP-4|DAP4|DLP4|SAPAP-4|SAPAP4JTK9|p59Hck|p61Hck
Cytomap

20q11.23

20q11.21

Type of geneprotein-codingprotein-coding
Descriptiondisks large-associated protein 4PSD-95/SAP90-binding protein 4SAP90/PSD-95-associated protein 4discs large homolog associated protein 4tyrosine-protein kinase HCKhematopoietic cell kinasehemopoietic cell kinasep59-HCK/p60-HCK
Modification date2020031320200329
UniProtAcc

Q9Y2H0

P08631

Ensembl transtripts involved in fusion geneENST00000475894, ENST00000339266, 
ENST00000340491, ENST00000373907, 
ENST00000373913, ENST00000401952, 
ENST00000534862, ENST00000375852, 
ENST00000375862, ENST00000518730, 
ENST00000520553, ENST00000538448, 
Fusion gene scores* DoF score18 X 16 X 9=259210 X 10 X 7=700
# samples 2314
** MAII scorelog2(23/2592*10)=-3.49435995194025
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(14/700*10)=-2.32192809488736
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: DLGAP4 [Title/Abstract] AND HCK [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointDLGAP4(35089913)-HCK(30659465), # samples:1
DLGAP4(35093770)-HCK(30659465), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneHCK

GO:0071801

regulation of podosome assembly

15998323

TgeneHCK

GO:2000251

positive regulation of actin cytoskeleton reorganization

15998323


check buttonFusion gene breakpoints across DLGAP4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across HCK (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4COADTCGA-AA-3812-01ADLGAP4chr20

35089913

+HCKchr20

30659465

+
ChimerDB4COADTCGA-AA-3812-01ADLGAP4chr20

35093770

+HCKchr20

30659465

+


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Fusion Gene ORF analysis for DLGAP4-HCK

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000475894ENST00000534862DLGAP4chr20

35089913

+HCKchr20

30659465

+
3UTR-5UTRENST00000475894ENST00000375852DLGAP4chr20

35089913

+HCKchr20

30659465

+
3UTR-5UTRENST00000475894ENST00000375862DLGAP4chr20

35089913

+HCKchr20

30659465

+
3UTR-5UTRENST00000475894ENST00000518730DLGAP4chr20

35089913

+HCKchr20

30659465

+
3UTR-5UTRENST00000475894ENST00000520553DLGAP4chr20

35089913

+HCKchr20

30659465

+
3UTR-5UTRENST00000475894ENST00000538448DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-3CDSENST00000339266ENST00000534862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-3CDSENST00000339266ENST00000534862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-3CDSENST00000340491ENST00000534862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-3CDSENST00000340491ENST00000534862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-3CDSENST00000373907ENST00000534862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-3CDSENST00000373907ENST00000534862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-3CDSENST00000373913ENST00000534862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-3CDSENST00000373913ENST00000534862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-3CDSENST00000401952ENST00000534862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-3CDSENST00000401952ENST00000534862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-3CDSENST00000475894ENST00000534862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000375852DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000375852DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000375862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000375862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000518730DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000518730DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000520553DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000520553DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000538448DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000339266ENST00000538448DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000375852DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000375852DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000375862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000375862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000518730DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000518730DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000520553DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000520553DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000538448DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000340491ENST00000538448DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000375852DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000375852DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000375862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000375862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000518730DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000518730DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000520553DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000520553DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000538448DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373907ENST00000538448DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000375852DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000375852DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000375862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000375862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000518730DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000518730DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000520553DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000520553DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000538448DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000373913ENST00000538448DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000375852DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000375852DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000375862DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000375862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000518730DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000518730DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000520553DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000520553DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000538448DLGAP4chr20

35089913

+HCKchr20

30659465

+
intron-5UTRENST00000401952ENST00000538448DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000475894ENST00000375852DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000475894ENST00000375862DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000475894ENST00000518730DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000475894ENST00000520553DLGAP4chr20

35093770

+HCKchr20

30659465

+
intron-5UTRENST00000475894ENST00000538448DLGAP4chr20

35093770

+HCKchr20

30659465

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for DLGAP4-HCK


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
DLGAP4chr2035089913+HCKchr2030659464+0.01183490.98816514
DLGAP4chr2035089913+HCKchr2030659464+0.01183490.98816514

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for DLGAP4-HCK


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:35089913/:30659465)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DLGAP4

Q9Y2H0

HCK

P08631

FUNCTION: May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.FUNCTION: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS. {ECO:0000269|PubMed:10092522, ECO:0000269|PubMed:10779760, ECO:0000269|PubMed:10973280, ECO:0000269|PubMed:11741929, ECO:0000269|PubMed:11896602, ECO:0000269|PubMed:12411494, ECO:0000269|PubMed:15010462, ECO:0000269|PubMed:15952790, ECO:0000269|PubMed:15998323, ECO:0000269|PubMed:17310994, ECO:0000269|PubMed:17535448, ECO:0000269|PubMed:19114024, ECO:0000269|PubMed:19903482, ECO:0000269|PubMed:20452982, ECO:0000269|PubMed:21338576, ECO:0000269|PubMed:7535819, ECO:0000269|PubMed:8132624, ECO:0000269|PubMed:9406996, ECO:0000269|PubMed:9407116}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for DLGAP4-HCK


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for DLGAP4-HCK


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for DLGAP4-HCK


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for DLGAP4-HCK


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource