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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:DNAJB2-NAP1L1 (FusionGDB2 ID:23426)

Fusion Gene Summary for DNAJB2-NAP1L1

check button Fusion gene summary
Fusion gene informationFusion gene name: DNAJB2-NAP1L1
Fusion gene ID: 23426
HgeneTgene
Gene symbol

DNAJB2

NAP1L1

Gene ID

3300

4673

Gene nameDnaJ heat shock protein family (Hsp40) member B2nucleosome assembly protein 1 like 1
SynonymsCMT2T|DSMA5|HSJ-1|HSJ1|HSPF3NAP1|NAP1L|NRP
Cytomap

2q35

12q21.2

Type of geneprotein-codingprotein-coding
DescriptiondnaJ homolog subfamily B member 2DnaJ (Hsp40) homolog, subfamily B, member 2dnaJ protein homolog 1heat shock 40 kDa protein 3heat shock protein J1heat shock protein, neuronal DNAJ-like 1nucleosome assembly protein 1-like 1HSP22-like protein interacting proteinNAP-1-related protein
Modification date2020032820200322
UniProtAcc

P25686

P55209

Ensembl transtripts involved in fusion geneENST00000463463, ENST00000336576, 
ENST00000392086, 		ENST00000261182, 
ENST00000393263, ENST00000535020, 
ENST00000542344, ENST00000544816, 
ENST00000549596, ENST00000431879, 
ENST00000547773, ENST00000547993, 
ENST00000548044, ENST00000552342, 
Fusion gene scores* DoF score3 X 3 X 2=1818 X 20 X 6=2160
# samples 322
** MAII scorelog2(3/18*10)=0.736965594166206
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(22/2160*10)=-3.29545588352617
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: DNAJB2 [Title/Abstract] AND NAP1L1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointDNAJB2(220146727)-NAP1L1(76453630), # samples:1
Anticipated loss of major functional domain due to fusion event.DNAJB2-NAP1L1 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
DNAJB2-NAP1L1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneDNAJB2

GO:0032091

negative regulation of protein binding

7957263

HgeneDNAJB2

GO:0032781

positive regulation of ATPase activity

7957263|22219199

HgeneDNAJB2

GO:0042026

protein refolding

21231916

HgeneDNAJB2

GO:0090084

negative regulation of inclusion body assembly

21231916

HgeneDNAJB2

GO:1903644

regulation of chaperone-mediated protein folding

7957263


check buttonFusion gene breakpoints across DNAJB2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across NAP1L1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/ABF967465DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-


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Fusion Gene ORF analysis for DNAJB2-NAP1L1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000463463ENST00000261182DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-3CDSENST00000463463ENST00000393263DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-5UTRENST00000463463ENST00000535020DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-5UTRENST00000463463ENST00000542344DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-5UTRENST00000463463ENST00000544816DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-5UTRENST00000463463ENST00000549596DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-intronENST00000463463ENST00000431879DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-intronENST00000463463ENST00000547773DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-intronENST00000463463ENST00000547993DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-intronENST00000463463ENST00000548044DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
3UTR-intronENST00000463463ENST00000552342DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000336576ENST00000535020DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000336576ENST00000542344DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000336576ENST00000544816DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000336576ENST00000549596DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000392086ENST00000535020DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000392086ENST00000542344DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000392086ENST00000544816DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-5UTRENST00000392086ENST00000549596DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000336576ENST00000431879DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000336576ENST00000547773DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000336576ENST00000547993DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000336576ENST00000548044DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000336576ENST00000552342DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000392086ENST00000431879DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000392086ENST00000547773DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000392086ENST00000547993DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000392086ENST00000548044DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
5CDS-intronENST00000392086ENST00000552342DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
Frame-shiftENST00000336576ENST00000261182DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
Frame-shiftENST00000336576ENST00000393263DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
Frame-shiftENST00000392086ENST00000261182DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-
Frame-shiftENST00000392086ENST00000393263DNAJB2chr2

220146727

+NAP1L1chr12

76453630

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for DNAJB2-NAP1L1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for DNAJB2-NAP1L1


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:220146727/:76453630)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DNAJB2

P25686

NAP1L1

P55209

FUNCTION: Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:7957263, PubMed:22219199). In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540). Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695). Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278). {ECO:0000269|PubMed:12754272, ECO:0000269|PubMed:15936278, ECO:0000269|PubMed:20889486, ECO:0000269|PubMed:21625540, ECO:0000269|PubMed:21719532, ECO:0000269|PubMed:22219199, ECO:0000269|PubMed:22396390, ECO:0000269|PubMed:24023695, ECO:0000269|PubMed:7957263}.FUNCTION: Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed:20002496, PubMed:26841755). Participates also in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed:28369616). Stimulates also homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed:24798879). Plays a key role in the regulation of embryonic neurogenesis (By similarity). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity). {ECO:0000250|UniProtKB:P28656, ECO:0000269|PubMed:20002496, ECO:0000269|PubMed:24798879, ECO:0000269|PubMed:26841755, ECO:0000269|PubMed:28369616}.; FUNCTION: (Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression. {ECO:0000269|PubMed:23691099}.; FUNCTION: (Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA. {ECO:0000269|PubMed:27599637}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for DNAJB2-NAP1L1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for DNAJB2-NAP1L1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for DNAJB2-NAP1L1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for DNAJB2-NAP1L1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource