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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:DRG1-LIMK2 (FusionGDB2 ID:24162) |
Fusion Gene Summary for DRG1-LIMK2 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: DRG1-LIMK2 | Fusion gene ID: 24162 | Hgene | Tgene | Gene symbol | DRG1 | LIMK2 | Gene ID | 51534 | 3985 |
| Gene name | vesicle trafficking 1 | LIM domain kinase 2 | |
| Synonyms | C6orf55|DRG-1|DRG1|HSPC228|LIP5|My012|SBP1 | - | |
| Cytomap | 6q24.1-q24.2 | 22q12.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | vacuolar protein sorting-associated protein VTA1 homologLYST-interacting protein 5SKD1-binding protein 1Vps20-associated 1 homologdopamine-responsive gene 1 proteinhomolog of mouse SKD1-binding protein 1vesicle (multivesicular body) trafficking 1 | LIM domain kinase 2 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q9Y295 | P53671 | |
| Ensembl transtripts involved in fusion gene | ENST00000331457, ENST00000433341, | ENST00000467301, ENST00000331728, ENST00000333611, ENST00000340552, ENST00000406516, ENST00000444929, | |
| Fusion gene scores | * DoF score | 5 X 7 X 5=175 | 9 X 9 X 5=405 |
| # samples | 7 | 9 | |
| ** MAII score | log2(7/175*10)=-1.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(9/405*10)=-2.16992500144231 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: DRG1 [Title/Abstract] AND LIMK2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | DRG1(31819396)-LIMK2(31667122), # samples:1 DRG1(31904362)-LIMK2(31654277), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | DRG1-LIMK2 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. DRG1-LIMK2 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. DRG1-LIMK2 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | LIMK2 | GO:0006468 | protein phosphorylation | 22328514 |
| Tgene | LIMK2 | GO:0030953 | astral microtubule organization | 22328514 |
| Fusion gene breakpoints across DRG1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across LIMK2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | ESCA | TCGA-LN-A5U7 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| ChimerDB4 | LGG | TCGA-CS-6666 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
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Fusion Gene ORF analysis for DRG1-LIMK2 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-intron | ENST00000331457 | ENST00000467301 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| Frame-shift | ENST00000331457 | ENST00000331728 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| Frame-shift | ENST00000331457 | ENST00000333611 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| Frame-shift | ENST00000331457 | ENST00000340552 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| Frame-shift | ENST00000331457 | ENST00000406516 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| Frame-shift | ENST00000331457 | ENST00000444929 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-3CDS | ENST00000433341 | ENST00000331728 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-3CDS | ENST00000433341 | ENST00000333611 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-3CDS | ENST00000433341 | ENST00000340552 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-3CDS | ENST00000433341 | ENST00000406516 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-3CDS | ENST00000433341 | ENST00000444929 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-5UTR | ENST00000331457 | ENST00000406516 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-5UTR | ENST00000433341 | ENST00000406516 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000331457 | ENST00000331728 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000331457 | ENST00000333611 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000331457 | ENST00000340552 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000331457 | ENST00000444929 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000331457 | ENST00000467301 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000433341 | ENST00000331728 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000433341 | ENST00000333611 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000433341 | ENST00000340552 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000433341 | ENST00000444929 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| intron-intron | ENST00000433341 | ENST00000467301 | DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667122 | + |
| intron-intron | ENST00000433341 | ENST00000467301 | DRG1 | chr22 | 31904362 | + | LIMK2 | chr22 | 31654277 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for DRG1-LIMK2 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667121 | + | 3.37E-12 | 1 |
| DRG1 | chr22 | 31819396 | + | LIMK2 | chr22 | 31667121 | + | 3.37E-12 | 1 |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for DRG1-LIMK2 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:31819396/:31667122) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| DRG1 | LIMK2 |
| FUNCTION: Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP (PubMed:29915238, PubMed:23711155). Appears to have an intrinsic GTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions (PubMed:23711155). When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation (PubMed:19819225, PubMed:29915238). Binds to microtubules and promotes microtubule polymerization and stability that are required for mitotic spindle assembly during prophase to anaphase transition. GTPase activity is not necessary for these microtubule-related functions (PubMed:28855639). {ECO:0000269|PubMed:19819225, ECO:0000269|PubMed:23711155, ECO:0000269|PubMed:28855639, ECO:0000269|PubMed:29915238}. | FUNCTION: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865). {ECO:0000269|PubMed:10436159, ECO:0000269|PubMed:11018042, ECO:0000269|PubMed:22328514, ECO:0000269|PubMed:25849865, ECO:0000269|PubMed:8537403}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for DRG1-LIMK2 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for DRG1-LIMK2 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for DRG1-LIMK2 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for DRG1-LIMK2 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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