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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:EIF2S1-RAD51B (FusionGDB2 ID:25731)

Fusion Gene Summary for EIF2S1-RAD51B

check button Fusion gene summary
Fusion gene informationFusion gene name: EIF2S1-RAD51B
Fusion gene ID: 25731
HgeneTgene
Gene symbol

EIF2S1

RAD51B

Gene ID

1965

5890

Gene nameeukaryotic translation initiation factor 2 subunit alphaRAD51 paralog B
SynonymsEIF-2|EIF-2A|EIF-2alpha|EIF2|EIF2AR51H2|RAD51L1|REC2
Cytomap

14q23.3

14q24.1

Type of geneprotein-codingprotein-coding
Descriptioneukaryotic translation initiation factor 2 subunit 1eIF-2-alphaeukaryotic translation initiation factor 2, subunit 1 alpha, 35kDaDNA repair protein RAD51 homolog 2RAD51 homolog BRecA-like proteinrecombination repair protein
Modification date2020031320200313
UniProtAcc

P05198

O15315

Ensembl transtripts involved in fusion geneENST00000256383, ENST00000466499, 
ENST00000469165, ENST00000390683, 
ENST00000471583, ENST00000487270, 
ENST00000487861, ENST00000488612, 
Fusion gene scores* DoF score4 X 5 X 5=10041 X 23 X 12=11316
# samples 856
** MAII scorelog2(8/100*10)=-0.321928094887362
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(56/11316*10)=-4.33679344445129
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: EIF2S1 [Title/Abstract] AND RAD51B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointEIF2S1(67847482)-RAD51B(68758600), # samples:1
EIF2S1(67847482)-RAD51B(68758601), # samples:1
Anticipated loss of major functional domain due to fusion event.EIF2S1-RAD51B seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
EIF2S1-RAD51B seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
EIF2S1-RAD51B seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across EIF2S1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across RAD51B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-CD-5800-01AEIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
ChimerDB4STADTCGA-CD-5800EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+


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Fusion Gene ORF analysis for EIF2S1-RAD51B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000256383ENST00000469165EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
5CDS-3UTRENST00000256383ENST00000469165EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
5CDS-3UTRENST00000466499ENST00000469165EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
5CDS-3UTRENST00000466499ENST00000469165EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000256383ENST00000390683EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000256383ENST00000390683EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000256383ENST00000471583EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000256383ENST00000471583EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000256383ENST00000487270EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000256383ENST00000487270EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000256383ENST00000487861EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000256383ENST00000487861EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000256383ENST00000488612EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000256383ENST00000488612EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000466499ENST00000390683EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000466499ENST00000390683EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000466499ENST00000471583EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000466499ENST00000471583EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000466499ENST00000487270EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000466499ENST00000487270EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000466499ENST00000487861EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000466499ENST00000487861EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+
Frame-shiftENST00000466499ENST00000488612EIF2S1chr14

67847482

+RAD51Bchr14

68758601

+
Frame-shiftENST00000466499ENST00000488612EIF2S1chr14

67847482

+RAD51Bchr14

68758600

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for EIF2S1-RAD51B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
EIF2S1chr1467847482+RAD51Bchr1468758600+0.0020285140.9979715
EIF2S1chr1467847482+RAD51Bchr1468758600+0.0020285140.9979715
EIF2S1chr1467847482+RAD51Bchr1468758600+0.0020285140.9979715
EIF2S1chr1467847482+RAD51Bchr1468758600+0.0020285140.9979715

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for EIF2S1-RAD51B


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:67847482/:68758600)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
EIF2S1

P05198

RAD51B

O15315

FUNCTION: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PubMed:16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex (PubMed:16289705). In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (PubMed:16289705). EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming (PubMed:19131336). {ECO:0000269|PubMed:16289705, ECO:0000269|PubMed:19131336}.FUNCTION: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. {ECO:0000269|PubMed:11751635, ECO:0000269|PubMed:11751636, ECO:0000269|PubMed:11842113, ECO:0000269|PubMed:12441335, ECO:0000269|PubMed:23108668, ECO:0000269|PubMed:23149936}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for EIF2S1-RAD51B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for EIF2S1-RAD51B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for EIF2S1-RAD51B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for EIF2S1-RAD51B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource